art%3A10.1134%2FS1022795412110130

sonamedo1000

EXPRESSION OF HETEROLOGOUS GENES IN PLANT SYSTEMS 1069

Comparative characteristics of stable and transient expression of genes in plants

Stable expression Transient expression

Characteristic

agrobacterial

transformation

Nuclear transformation

bioballistic

transformation

protoplast

transformation

Plastid

transformation

bioballistic

transformation

agroinfiltration viral infection

Dependence on genotype Dependent Less dependent Dependent Less dependent Dependent Dependent

Host plant range Wide Wide Limited Limited Limited Limited

Vectors Complex Simple Simple Complex Complex Complex

Possibility to use linear DNA No Yes Yes Yes No No

Transfer of genes with a size

of over 1000 Kb

Possible Possible Possible Impossible Possible Possible

Copy number (number

of insertions)

Low copy number Multiple T-DNA

integrations and rearrangements

Low copy number Single copy? Without integration Without integration

Posttranslational modifications

of protein products

Possible Possible Possible Impossible Possible Possible

Expression level Medium Low Low High** High High

Transformation efficiency High* Medium Low Very low High High

Specific expensive equipment Not required Required Not required Required Not required Not required

Complexity of procedures Simple Simple Complex Complex Simple Simple

Biosafety Relative*** Relative*** Relative*** High**** High***** High*****

* High transformation efficiency for some species, but special protocols are required.

** Usually from several percent of the total soluble protein to the physiological maximum >50% of the total soluble protein.

*** Special approaches are required to prevent the release of a transgene into the environment.

**** Maternal inheritance of plastids, the pollen is free of plastids.

***** Transgenic plants are not generated. Most recombinant proteins can be produced under controlled conditions.

RUSSIAN JOURNAL OF GENETICS Vol. 48 No. 11 2012