art%3A10.1134%2FS1022795412110130

sonamedo1000

EXPRESSION OF HETEROLOGOUS GENES IN PLANT SYSTEMS 1071

Creation

of an expression vector

Isolation of tobacco

mesophyll protoplasts

Direct transformation of tobacco

protoplasts with PEG

K

T

Formation of cell colonies

on a selective medium

Callus formation and morphogenesis

Rooting on a selective medium

Molecular genetic analysis

T 0

T

K

Transplantation into soil, seed production

T 1

Segregation analysis

T 2

Analysis of stable transgene

heritability in generations

T

Morphological and biochemical

3 K T

characteristics

Fig. 2. A schematic diagram of generation of transgenic plants with the use of protoplast transformation. K, control; T, transgene.

RUSSIAN JOURNAL OF GENETICS Vol. 48 No. 11 2012