Untersuchungen zu familiären und rassespezifischen ...

software (multipoint engine for rapid likelihood inference, version 1.1) (Abecasis et
al., 2002). The Zmean and LOD score test statistics were used to test for the
proportion of alleles shared by affected individuals identical by descent (IBD) for the
considered marker loci (Kong and Cox, 1997; Kruglyak et al., 1996; Whittemore et
al., 1994). The best option of MERLIN was used to estimate haplotypes.
The CASECONTROL procedure of SAS/Genetics, version 9.2 (Statistical Analysis
System, Cary, NC, USA) based on χ 2 -tests for genotypes, alleles and trends of the
alleles was used for association testing. For estimation of allele frequencies and to
test genotype frequencies for Hardy-Weinberg equilibrium the ALLELE procedure of
SAS/Genetics was employed. Moreover, we used the HAPLOTYPE procedure of
SAS/Genetics to test for trait-marker association.
Results and discussion
Neither the c.3G>A SNP found in a kindred of Australian Shepherds nor the c.1113-
1145del mutation described in Giant Schnauzers could be identified in this 17
Norwegian lundehunds. All genotyped lundehunds were homozygous for the wildtype
alleles.
We developed eleven microsatellite markers flanking AMN. Six of these markers
were homozygous for all 17 genotyped lundehunds, and five microsatellites were
polymorphic. The results of the tests for Hardy-Weinberg equilibrium, the observed
levels of heterozygosity (HET), polymorphism information content (PIC) and allelic
diversity for the developed microsatellite markers are shown in Table 4. The non-
parametric multipoint linkage analysis did not show significant Zmeans or LOD
scores.