In-vitro-Maturation porciner Oozyten auf Feederlayer ... - Dragon IVF
In-vitro-Maturation porciner Oozyten auf Feederlayer ... - Dragon IVF
In-vitro-Maturation porciner Oozyten auf Feederlayer ... - Dragon IVF
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parameters in those cultures where a feeder layer was not used during the embryonal<br />
culture phase, it may be concluded that within the scope of this research work, feeder<br />
layers have a positive effect on both these phases of oocyte cultivation.<br />
<strong>In</strong> Experiment 9, in <strong>vitro</strong> matured oocytes were compared with in vivo matured oocytes<br />
(n = 127-130 oocytes/group). The immature oocytes were matured in <strong>vitro</strong> on oviduct<br />
feeder layers and then cultivated, while the mature oocytes were collected from<br />
superovulated sows. The success of the different maturation systems were then tested<br />
by embryo transfer. The mature oocytes in each group were fertilised in <strong>vitro</strong> and the<br />
resulting embryos were implanted in synchronised recipient sows. A viable pregnancy<br />
could not be produced in any of the test groups.<br />
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