In-vitro-Maturation porciner Oozyten auf Feederlayer ... - Dragon IVF

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In-vitro-Maturation porciner Oozyten auf Feederlayer ... - Dragon IVF

parameters in those cultures where a feeder layer was not used during the embryonal

culture phase, it may be concluded that within the scope of this research work, feeder

layers have a positive effect on both these phases of oocyte cultivation.

In Experiment 9, in vitro matured oocytes were compared with in vivo matured oocytes

(n = 127-130 oocytes/group). The immature oocytes were matured in vitro on oviduct

feeder layers and then cultivated, while the mature oocytes were collected from

superovulated sows. The success of the different maturation systems were then tested

by embryo transfer. The mature oocytes in each group were fertilised in vitro and the

resulting embryos were implanted in synchronised recipient sows. A viable pregnancy

could not be produced in any of the test groups.

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