Zellbiologie & Imaging - Laborwelt

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Zellbiologie & Imaging - Laborwelt

Normalized Normalized Cell Index Cell Index

Cells in Real-Time!

5

Cells in Real-Time!

Control

4

12.5 �M Etoposide

5

3

Control 25 �M Etoposide

4

Treatment

12.5 �M Etoposide

2

3

1

Seed

Cells

Treatment

25 �M Etoposide

50 �M Etoposide

2

Seed

0

Cells

1 0 20 40 60

100 �M Etoposide

50 �M Etoposide 80

0

Time (hours)

100 �M Etoposide

0 20 40

Time (hours)

60 80

xCELLigence Real-Time

Cell xCELLigence Analyzer Real-Time System

Cell Analyzer System

RTCA SP InstrumentNEW

RTCA SP InstrumentNEW

Greater Insight, True Understanding

Greater Insight, True Understanding

Figure 1: Real-time monitoring of cytotoxicity

through DNA damage. Etoposide is a DNA damaging

agent Figure which 1: Real-time induces apoptosis monitoring in high of cytotoxicity concentrations,

while through at lower DNA concentrations damage. Etoposide it leads is a to DNA S-Phase damaging and/or

G2 agent arrest. which induces apoptosis in high concentrations,

while at lower concentrations it leads to S-Phase and/or

G2 arrest.

XCELLIGENCE is a trademark of Roche.

© 2008 Roche Diagnostics GmbH. All rights reserved.

XCELLIGENCE is a trademark of Roche.

© 2008 Roche Diagnostics GmbH. All rights reserved.

www.roche-applied-science.com

www.roche-applied-science.com

Experience the power of dynamic, real-time, label-free cellular

analysis with the xCELLigence System from Roche Applied Science.

Experience the power of dynamic, real-time, label-free cellular

Acquire data that end-point analysis could never realize, throughout

analysis with the xCELLigence System from Roche Applied Science.

your entire experiment. Work label-free to ensure physiologically

Acquire data that end-point analysis could never realize, throughout

relevant data, and choose from flexible throughput options to meet

your entire experiment. Work label-free to ensure physiologically

your needs: 24, 96, or 576 (6 x 96) wells simultaneously.

relevant data, and choose from flexible throughput options to meet

your needs: 24, 96, or 576 (6 x 96) wells simultaneously.

� Capture Real-Time data throughout the entire experiment

� Obtain physiologically relevant data without labels and reporters

� Capture Real-Time data throughout the entire experiment

� Analyze cells across a broad dynamic range

� Obtain physiologically relevant data without labels and reporters

� Perform a wide variety of applications

� Analyze cells across a broad dynamic range

(e.g. proliferation and cytotoxicity, Figure 1)

� Perform a wide variety of applications

(e.g. proliferation and cytotoxicity, Figure 1)

For more information, visit www.xcelligence.roche.com or

contact your local Roche representative today!

For more information, visit www.xcelligence.roche.com or

contact your local Roche representative today!

Roche Diagnostics GmbH

Roche Applied Science

68298 Roche Mannheim, Diagnostics Germany GmbH

Roche Applied Science

68298 Mannheim, Germany