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BIOCHEMICAL AND HAEMATOLOGICAL PROFILE IN THE
ADVANCED GESTATION PERIOD OF COPPER DEFICIENT HOLSTEIN
AND BROWN SWISS CATTLE
Alina ANTON, Gheorghe SOLCAN, Vasile BOGHIAN, Nicolae HAGIU
Faculty of Veterinary Medicine Iasi
Alley Mihail Sadoveanu nr.8; Iasi – 700489, Romania; antonclaraalina@yahoo.com
10 Holstein and 10 Brown Swiss cattle in advanced gestation period, clinically healthy, from a
farm from Romania were divided into 2 groups according to breed (group 1 = Holstein, group 2 =
Brown Swiss) with the aim of evaluating the copper status and correlating these status with
haematological and biochemical profiles.Ceruloplasmin concentrations (the primary Cu‐
containing component of the blood) have been considered as reliable indicators to copper
status.During the protocol, no statistically significant differences were noted in the hematological
and biochemical parameters in Holstein versus Brown Swiss cattle, remaining within their
physiological adult reference range, except plasma ceruloplasmin.These were below cattle
reference range for all 20 cattles.
In our study, the values of plasma ceruloplasmin were significantly increased (P < 0,05) in Brown
Swiss cattle (54.97 ± 15.62 mg/L) than Holstein cattle (40.60 ± 5.18 mg/L), probably due to
genetic predisposition.
Keywords: ceruloplasmin, copper, haematology, blood biochemistry, cattle
Copper is the rate – limiting element in the synyhesis of ceruloplasmin, a glycoprotein that is
synthetized in the liver. Ceruloplasmin concentrations (the primary Cu‐containing component
of the blood) have been considered as reliable indicators to copper status (Jain, 1993). Close
correlation between plasma ceruloplasmine and copper concentration have been reported in
many animal species in cluding sheep, cattle and horses (Arthington et al., 1996; Cerone et al.,
1998).
The aim of the present study was to determine the copper status and the correlation between
these status and haematological and biochemical profiles.
MATERIAL AND METHODS
The present study was carried out on a private farm from Nord‐East of Romania.
Blood samples were collected from 20 cattle in the advanced gestation period grouped by
breeds (group 1 = Holstein, group 2 = Brown Swiss). The animals were clinically normal and
free from any external, and internal parasites. They were fed with corn silage, concentrates
and hay. The animals were supplemented with 250 g/day of a commercial mineral and vitamin
mix. The basal diet was analyzed to contain 9 mg of copper/kg dry matter intake.
Blood samples were collected from the coccygeal vein into heparinized vacutainers for plasma
and uncoated vacutainers for serum. The samples were analysed for glucose, urea nitrogen
(BUN), β‐hydroxybutyrate (β‐OHB), cholesterol, calcium (Ca), phosphorus (P), magnesium
(Mg), alkaline phosphatase (PA), total protein (Pt), albumin (Alb), globulin (Gb), plasma
ceruloplasmin (Cerlp) and plasma haptoglobin (Hapt). Serum levels analysis and plasma were
performed in an automatic biochemical Cormay Accent 200.
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