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Gene Cloning and DNA Analysis: An Introduction, Sixth Edition ...

Gene Cloning and DNA Analysis: An Introduction, Sixth Edition ...

Gene Cloning and DNA Analysis: An Introduction, Sixth Edition

  • Page 4: GENE CLONING AND DNA ANALYSIS
  • Page 10: This edition first published 2010,
  • Page 16: Contents CONTENTS Preface to the Si
  • Page 20: Contents ix 4.3 Ligation—joining
  • Page 24: Contents xi 8 How to Obtain a Clone
  • Page 28: Contents xiii 11.3 Identifying and
  • Page 32: Contents xv 15.1.2 Herbicide resist
  • Page 36: PART I The Basic Principles of Gene
  • Page 42: 4 Part I The Basic Principles of Ge
  • Page 46: 6 1.4 What is PCR? Figure 1.2 The b
  • Page 50: 8 Figure 1.3 Cloning allows individ
  • Page 54:

    10 Figure 1.5 Gene isolation by PCR

  • Page 58:

    12 Further reading FURTHER READING

  • Page 62:

    14 Figure 2.1 Plasmids: independent

  • Page 66:

    16 Figure 2.4 Plasmid transfer by c

  • Page 70:

    18 Figure 2.6 Capsid components Pha

  • Page 74:

    20 Figure 2.7 λ phage particle att

  • Page 78:

    22 (a) The linear form of the λ DN

  • Page 82:

    24 Part I The Basic Principles of G

  • Page 86:

    26 Bacterial culture Table 3.1 Cent

  • Page 90:

    28 Bacterial culture Figure 3.3 Har

  • Page 94:

    30 Figure 3.6 Removal of protein co

  • Page 98:

    32 Figure 3.8 Collecting DNA by eth

  • Page 102:

    34 Figure 3.10 DNA purification by

  • Page 106:

    36 Figure 3.12 Two conformations of

  • Page 110:

    38 Figure 3.15 Partial unwinding of

  • Page 114:

    40 Figure 3.18 Preparation of a pha

  • Page 118:

    42 Figure 3.21 Collection of phage

  • Page 122:

    44 Further reading FURTHER READING

  • Page 126:

    46 Part I The Basic Principles of G

  • Page 130:

    48 Figure 4.3 The reactions catalyz

  • Page 134:

    50 Figure 4.6 (a) Alkaline phosphat

  • Page 138:

    52 Figure 4.8 (a) Restriction of ph

  • Page 142:

    54 Figure 4.9 (a) Production of blu

  • Page 146:

    56 Table 4.2 A 10 × buffer suitabl

  • Page 150:

    58 Figure 4.13 Visualizing DNA band

  • Page 154:

    60 Figure 4.15 Using a restriction

  • Page 158:

    62 Figure 4.17 The influence of DNA

  • Page 162:

    64 Figure 4.20 (a) Ligating blunt e

  • Page 166:

    66 Figure 4.23 Adaptors and the pot

  • Page 170:

    68 Figure 4.25 The use of adaptors:

  • Page 174:

    70 Figure 4.28 (a) The ends of the

  • Page 178:

    72 Chapter 5 Introduction of DNA in

  • Page 182:

    74 Part I The Basic Principles of G

  • Page 186:

    76 Figure 5.4 Normal E. coli cell (

  • Page 190:

    78 Figure 5.8 (b) Replica plating W

  • Page 194:

    80 Figure 5.10 (a) The role of the

  • Page 198:

    82 Figure 5.11 (a) A single-strain

  • Page 202:

    84 Figure 5.13 Strategies for the s

  • Page 206:

    86 Figure 5.14 Figure 5.15 (a) Prec

  • Page 210:

    88 Chapter 6 Cloning Vectors for E.

  • Page 214:

    90 during purification. pBR322 is 4

  • Page 218:

    92 (a) pUC8 (b) Restriction sites i

  • Page 222:

    94 Figure 6.5 The M13 genome, showi

  • Page 226:

    96 not totally disrupt the lacZ′

  • Page 230:

    98 Figure 6.10 The λ genetic map,

  • Page 234:

    100 (a) Cloning with a λ replaceme

  • Page 238:

    102 Figure 6.15 (a) A typical cosmi

  • Page 242:

    104 capsid structure. Cosmid-type v

  • Page 246:

    106 Figure 7.1 The yeast 2 µm plas

  • Page 250:

    108 Figure 7.4 Cloning with an E. c

  • Page 254:

    110 Figure 7.7 Chromosome structure

  • Page 258:

    112 for instance to examine the seg

  • Page 262:

    114 Figure 7.10 The Ti plasmid and

  • Page 266:

    116 (a) Wound infection by recombin

  • Page 270:

    118 Figure 7.15 Direct gene transfe

  • Page 274:

    120 Caulimovirus vectors Although o

  • Page 278:

    122 Figure 7.18 Cloning in Drosophi

  • Page 282:

    124 cause the death of the mouse ce

  • Page 286:

    126 Chapter 8 How to Obtain a Clone

  • Page 290:

    128 Figure 8.2 The basic strategies

  • Page 294:

    130 Figure 8.4 (a) Construction of

  • Page 298:

    132 Figure 8.5 Preparation of a gen

  • Page 302:

    134 Figure 8.7 One possible scheme

  • Page 306:

    136 Figure 8.10 The structure of α

  • Page 310:

    138 Figure 8.12 Two methods for the

  • Page 314:

    140 Figure 8.15 A simplified scheme

  • Page 318:

    142 Figure 8.17 Heterologous probin

  • Page 322:

    144 skills (Figure 8.19b). The same

  • Page 326:

    146 The problem of gene expression

  • Page 330:

    148 Figure 9.1 Hybridization of the

  • Page 334:

    150 Figure 9.3 Figure 9.4 5’ 3’

  • Page 338:

    152 Figure 9.7 A typical temperatur

  • Page 342:

    154 Figure 9.9 Calculating the T m

  • Page 346:

    156 Figure 9.13 5’ 3’ A G A C T

  • Page 350:

    158 Figure 9.16 Double-stranded PCR

  • Page 354:

    160 Figure 9.18 Hybridization of a

  • Page 360:

    PART II The Applications of Gene Cl

  • Page 366:

    166 termination sequencing has gain

  • Page 370:

    168 Figure 10.3 Reading the sequenc

  • Page 374:

    170 Figure 10.5 The basis to therma

  • Page 378:

    172 Figure 10.7 Pyrosequencing. Fig

  • Page 382:

    174 Figure 10.9 Part II The Applica

  • Page 386:

    176 Figure 10.11 Using oligonucleot

  • Page 390:

    178 Figure 10.13 Chromosome walking

  • Page 394:

    180 Figure 10.16 Part II The Applic

  • Page 398:

    182 Figure 10.20 Fluorescent in sit

  • Page 402:

    184 s Part II The Applications of G

  • Page 406:

    186 Figure 11.1 Some fundamentals o

  • Page 410:

    188 Figure 11.3 The 5′ end of an

  • Page 414:

    190 Figure 11.6 5' 3' RNA transcrip

  • Page 418:

    192 Figure 11.8 Possible positions

  • Page 422:

    194 Figure 11.11 A bound protein pr

  • Page 426:

    196 Figure 11.14 A modification int

  • Page 430:

    198 Table 11.1 Figure 11.16 A repor

  • Page 434:

    200 Pure mRNA Labelled translation

  • Page 438:

    202 Figure 11.22 (a) Restriction fr

  • Page 442:

    204 Part II The Applications of Gen

  • Page 446:

    206 Part II The Applications of Gen

  • Page 450:

    208 as molecular biology in silico,

  • Page 454:

    210 Figure 12.4 Part II The Applica

  • Page 458:

    212 Figure 12.7 Using comparisons b

  • Page 462:

    214 Figure 12.10 The use of a yeast

  • Page 466:

    216 Figure 12.11 Serial analysis of

  • Page 470:

    218 Load the protein sample Figure

  • Page 474:

    220 Figure 12.16 Analyzing two prot

  • Page 478:

    222 Part II The Applications of Gen

  • Page 484:

    Chapter 13 Production of Protein fr

  • Page 488:

    Chapter 13 Production of Protein fr

  • Page 492:

    Chapter 13 Production of Protein fr

  • Page 496:

    Chapter 13 Production of Protein fr

  • Page 500:

    Chapter 13 Production of Protein fr

  • Page 504:

    Chapter 13 Production of Protein fr

  • Page 508:

    Chapter 13 Production of Protein fr

  • Page 512:

    Chapter 13 Production of Protein fr

  • Page 516:

    Chapter 13 Production of Protein fr

  • Page 520:

    Chapter 13 Production of Protein fr

  • Page 524:

    Chapter 14 Gene Cloning and DNA Ana

  • Page 528:

    Chapter 14 Gene Cloning and DNA Ana

  • Page 532:

    Chapter 14 Gene Cloning and DNA Ana

  • Page 536:

    Chapter 14 Gene Cloning and DNA Ana

  • Page 540:

    Chapter 14 Gene Cloning and DNA Ana

  • Page 544:

    Chapter 14 Gene Cloning and DNA Ana

  • Page 548:

    Chapter 14 Gene Cloning and DNA Ana

  • Page 552:

    Chapter 14 Gene Cloning and DNA Ana

  • Page 556:

    Chapter 14 Gene Cloning and DNA Ana

  • Page 560:

    Chapter 14 Gene Cloning and DNA Ana

  • Page 564:

    Chapter 15 Gene Cloning and DNA Ana

  • Page 568:

    Chapter 15 Gene Cloning and DNA Ana

  • Page 572:

    Chapter 15 Gene Cloning and DNA Ana

  • Page 576:

    Chapter 15 Gene Cloning and DNA Ana

  • Page 580:

    Chapter 15 Gene Cloning and DNA Ana

  • Page 584:

    Chapter 15 Gene Cloning and DNA Ana

  • Page 588:

    Chapter 15 Gene Cloning and DNA Ana

  • Page 592:

    Chapter 15 Gene Cloning and DNA Ana

  • Page 596:

    Chapter 15 Gene Cloning and DNA Ana

  • Page 600:

    Chapter 16 Gene Cloning and DNA Ana

  • Page 604:

    Chapter 16 Gene Cloning and DNA Ana

  • Page 608:

    Chapter 16 Gene Cloning and DNA Ana

  • Page 612:

    Chapter 16 Gene Cloning and DNA Ana

  • Page 616:

    Chapter 16 Gene Cloning and DNA Ana

  • Page 620:

    Chapter 16 Gene Cloning and DNA Ana

  • Page 624:

    Chapter 16 Gene Cloning and DNA Ana

  • Page 628:

    Chapter 16 Gene Cloning and DNA Ana

  • Page 632:

    Glossary 299 Bacteriophage or phage

  • Page 636:

    Glossary 301 CpG island A GC-rich D

  • Page 640:

    Glossary 303 Genetic map A genome m

  • Page 644:

    Glossary 305 Liposome A lipid vesic

  • Page 648:

    Glossary 307 Pharming Genetic modif

  • Page 652:

    Glossary 309 Restriction analysis D

  • Page 656:

    Glossary 311 Transgenic Referring t

  • Page 660:

    Index 313 linear and circular forms

  • Page 664:

    Index 315 bacteriophages, 19-23 clo

  • Page 668:

    Index 317 Mendel, G., 3 messenger R

  • Page 672:

    Index 319 ribosome inactivating pro

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