Views
3 years ago

Cloned Enzyme Donor Immunoassay (CEDIA ... - Clinical Chemistry

Cloned Enzyme Donor Immunoassay (CEDIA ... - Clinical Chemistry

Cloned Enzyme Donor Immunoassay (CEDIA ... - Clinical

CLIN. CHEM. 41/1, 92-98 (1995) #{149} Drug Monitoring and Toxicology Cloned Enzyme Donor Immunoassay (CEDIA) for Drugs-of-Abuse Screening David A. Armbruster,’ Edward C. Hubster, Melvin S. Kaufman, and Monica K. Ramon Large numbersofspecimens(5000-18 000) were screened for amphetamines,barbiturates, cocaine,manjuana,opiates, and phencyclidine by RIA (Roche),EmitII(Syva), and a new immunoassay,CEDIA (clonedenzyme donorimmunoassay, Microgenics). Allimmunoassaysperformedequivalently for cocaine, opiates, and phencydidine. All immunoassaysdetected the same amphetamine/methamphetamine-positive specimens,but all also detected numerousspecimenscontainingcross-reacting sympathomimetic amines.CEDL detected 100%, Emit1193%, and RIA 82% ofthebarbituratepositive specimens. EmitIIand CEDIA detected86-88% of the specimensfound by RIA to be manjuana positive. A subset of specimens was additionally screened by OnUne (Roche) and TDx (Abbott) for amphetamines, cocaine, and marijuana. OnLine arid TDx also detected all of the amphetamine-positive specimens and numerous specimens containing cross-reacting sympathomimetic amines. All immunoassays performed equivalently for cocaine, and the four nonisotopic tests detected 86-89% ofthe marijuana positives foundby AlA. Interfering sympathomimetic amine drug compounds can be eliminated by using an oxidizing agent, thus decreasing the number of unconfirmable amphetamine presumptive positives. The CEDIAs for all of the major drugs of abuse are reliable and effective for large-volume urine screening programs. Indexing Terms: marijuana/cocaine/opiates/amphe famines/barbit urates/phencyclidine/intermethod comparison/urine Testing urine specimens for drugs of abuse is routinely performed by private industry in workplace drug-testing programs; such testing is mandatory for federal employees. A two-tiered approach is used: All specimens are screened with an immunoassay and the screen-presumptive positives are confirmed by gas chromatography-mass spectrometry (GC-MS).2 The screening test detects the presence of drug and (or) drug metabolite(s) at or above a stated administrative cutoff concentration, and the GC-MS procedures, using separate cutoff values, specifically confirm the presence of the drug. Screening immunoassays are ideally simple, rapid, inexpensive, and capable of automation for large-volume testing. Immunoassays must be accurate Armstrong Laboratory Drug Testing Division, Human Systems Center (AFMC), 2601 West Road, Suite 1, Brooks AFB, TX 78235- 3219. 1 Corresponding author. Fax 210-536-3219. 2Nonstandard abbreviations: GC-MS, gas chromatographymass spectrometry; DOD, Department of Defense; EIA, enzyme immunoassay; and CEDIA, cloned enzyme donor immunoassay. The opinions expressed herein are those of the authors alone and do not necessarily reflect the views of the Department of Defense or of the US Air Force. Received May 31, 1994; accepted October 7, 1994. 92 CLINICALCHEMISTRY,Vol.41, No. 1, 1995 and precise to detect the majority of positive specimens and be reliable to serve as an effective deterrent of drug abuse. We previously evaluated the most commonly used screening tests, including the most recent enzyme immunoassay (EIA) from Syva, Emit II, and a new family of screening tests, Roche’s OnLine assays, based on the kinetic interaction of microparticles in solution (1-3); our purpose was to determine whether these nonisotopic assays would be suitable replacements for the RIA tests currently required by the Department of Defense (DOD) for the military drug-testing program. Another family of immunoassays-based on the cloned enzyme donor iimnunoassay (CEDJA) methodolo-has recently been developed by Microgenics Corporation, and we report here our experience with them. We screened many thousands of specimens for amphetamines, barbiturates, cocaine, marijuana, opiates, and phencycidine, using our routine RJA tests, Emit II, and CEDIA. All specimens that gave positive results by these assays (“presumptive positive”) were tested by GC-MS for confirmation. Most of the specimens screening positive for marijuana, cocaine, and amphetamines were also tested with the OnLine tests and the Abbott TDx fluorescence polarization immunoassay. Materials and Methods Immunoassay Screen Procedure Batches of 200 urine specimens submitted to our laboratory from Air Force units in this country and around the world were tested by routine RIA procedures in accordance with the DOD drug-testing program. Each batch was analyzed for marijuana, cocaine, and a third drug-either amphetamines, barbiturates, opiates, or phencycidine, the selection rotating monthly. After allquots were released from the forensic chain of custody, they were analyzed for the same drugs with a Hitachi 717 analyzer (Boehringer Mannheim, Indianapolis, IN) and Emit II and CEDIA reagents. Aliquots were tested for the same drugs with both types of nonisotopic tests at the same time. Presumptive positives detected by any immunoassay were submitted for GC-MS confirmation. Because of the impracticality of screening all specimens by five different immunoassays, only the presumptive positives detected by RIA, Emit II, or CEDIA were tested with OnLine and TDx assays. DOD screening immunoassay and GC-MS cutoff values are listed in Table 1. Note that some DOD screen cutoffs differ from those mandated by the Department of Health and Human Services National Laboratory Certification Program.

PDF Practical Clinical Hypnosis: Technique and Applications (Scientific Foundations of Clinical Counseling and Psychology) FREE BOOK ONLINE
normand.kemp
PDF Clinical Repertory to the Dictonary of Materia Medica BOOK ONLINE
ivor.sylvester
FREE PDF Clinical Aromatherapy: Essential Oils in Practice, Second Edition BOOK ONLINE
grier123
Best book Chemistry (DK Eyewitness, 79) BOOK ONLINE
nachmen93
liberty books Chemistry For Every Kid (Turtleback School Library Binding Edition) BOOK ONLINE
tionni34
liberty book Chemistry For Every Kid (Turtleback School Library Binding Edition) [DOWNLOAD] ONLINE
tionni34
GET PDFbook Janice VanCleave s Chemistry for Every Kid: 101 Easy Experiments that Really Work READ ONLINE
makhi5
Free [PDF] Downlaod International Handbook of Clinical Hypnosis READ ONLINE
dex.upton
Free [PDF] Downlaod Clinical Materia Medica FREE BOOK ONLINE
marcus.trafford
Free [PDF] Downlaod Becoming a Reflective Practitioner: A Reflective and Holistic Approach to Clinical Nursing, Practice Develment and Clinical Supervision FREE BOOK ONLINE
yorick.tayle
DOWNLOAD NOW Trancework: An Introduction to Clinical Hypnosis READ ONLINE
addison.dolph
DOWNLOAD NOW Autogenic Training: A Clinical Guide DOWNLOAD ONLINE
addison.dolph
DOWNLOAD NOW Lectures on Clinical Materia Medica in Family Order BOOK ONLINE
kole.percival
DOWNLOAD NOW Compresses and other Therapeutic Applications: A Handbook from the Ita Wegman Clinic READ ONLINE
german.dennyss
Free [PDF] Downlaod Integrative Hypnotherapy: Complementary approaches in clinical care, 1e READ ONLINE
norwood.irwin
PDF A Compend of the Principles of Homeopathy as Taught by Hahnemann and Verified by a Century of Clinical Application FREE BOOK ONLINE
timothy.will
FREE PDF A Compend of the Principles of Homeopathy as Taught by Hahnemann and Verified by a Century of Clinical Application BOOK ONLINE
timothy.will
DOWNLOAD NOW A Compend of the Principles of Homeopathy as Taught by Hahnemann and Verified by a Century of Clinical Application BOOK ONLINE
timothy.will
PDF Ethics in Psychology and the Mental Health Professions: Standards and Cases (Oxford Textbooks in Clinical Psychology) DOWNLOAD ONLINE
yispuyes
DOWNLOAD NOW Nutrient Power: Heal Your Biochemistry and Heal Your Brain FREE BOOK ONLINE
cletis.grover
PDF Candida Albicans: Natural Remedies for Yeast Infection BOOK ONLINE
sean.roderick
Enzyme ImmunoassayMethod for ... - Clinical Chemistry
clinchem.org
Thin-layer Chromatography and Enzyme ... - Clinical Chemistry
clinchem.org
Development and validation of a sensitive enzyme immunoassay for ...
weihenstephan.de
Mitochondrial Enzymes in Human Serum ... - Clinical Chemistry
clinchem.org
PROLACTIN ENZYME IMMUNOASSAY TEST KIT Enzyme ... - Linear
linear.es
A Rapid Enzyme Immunoassay for the Detection of IgM to ...
simoco.dk
TRIIODOTHYRONINE (T3) ENZYME IMMUNOASSAY TEST KIT ...
linear.es
Comparison of three enzyme immunoassays for measuring 17beta ...
dairy.ifas.ufl.edu