role of th1 and th17 cd4+ t cell subsets in the pathogenesis of ...

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MBP – Myelin basic protein MCP‐1 – Monocyte chemoattractant protein‐1 MCS – Multiple cloning site MHC – Major histocompatibility complex MOG – Myelin oligodendrocyte glycoprotein MRI – Magnetic resonance imaging MS – Multiple clerosis NK – Natural killer NHR – Nuclear hormone receptor NR2F6 – Nuclear orphan receptor subfamily 2, group F, member 6 PCR – polymerase chain reaction PLP – Proteolipid protein PNS – Peripheral nervous system PPAR – Peroxisome proliferator‐activated receptor PE – Phycoerythrin PercP ‐ Peridinin chlorophyll protein RAG – Recombination‐activating gene RAR – Nuclear retinoic acid receptor‐alpha RFP – Red fluorescent protein ROR – Retinoic acid receptor‐related orphan receptor RORE – ROR responsive element SPF – Specific pathogen‐free STAT – Signal transducer and activator of transcription Tbet – T box expressed in T cells TCR – T cell receptor Tg – Transgenic TGF − Transforming growth factor Th – Helper T cell TLRs – Toll‐like receptors TNF – Tumor necrosis factor Tregs – Regulatory T cells VCAM‐1 – Vascular cell adhesion molecule‐1 VLA4 – Very late antigen 4 6
PUBLICATIONS During the development of the PhD thesis, I participated in other group projects, which led to significant publications in international peer‐reviewed journals: - Krishnamoorthy G, Saxena A, Mars LT, Domingues HS, Mentele R, Ben-Nun A, Lassmann H, Dornmair K, Kurschus FC, Liblau R and Wekerle H. (2009). Myelin‐specific T cells also recognize neuronal autoantigen in a transgenic mouse model of multiple sclerosis. Nature Medicine 15: 626‐632. - Pöllinger B, Krishnamoorthy G, Berer K, Lassmann H, Bösl MR, Dunn M, Domingues HS, Holz A, Kurschus FC and Wekerle H (2009). Spontaneous relapsing‐remitting EAE in the SJL/J mouse: MOG‐reactive transgenic T cells recruit endogenous MOG‐specific B cells. Journal of Experimental Medicine 206: 1303‐1316. In addition, the results described in chapters 3 and 4 of this thesis are being prepared for publication submission: - Domingues HS, Mues M, Lassmann H, Krishnamoorthy G and Wekerle H. Differential pathogenic competence of Th1 and Th17 cells in experimental autoimmune encephalomyelitis. 7
Page 1: Helena Sofia de Azevedo Domingues T
Page 4 and 5: Ferreira e Ricardo Marques pela mot
Page 7 and 8: TABLE OF CONTENTS Acknowledgments /
Page 9 and 10: 2.2.13 Quantitative real‐time PCR
Page 11: ABBREVIATIONS aa - Amino acid ADCC
Page 17 and 18: SUMMARY Multiple sclerosis (MS) is
Page 19 and 20: RESUMO A Esclerose Múltipla (EM)
Page 21: OBJECTIVES The main aim of this the
Page 25 and 26: CHAPTER 1 ‐ INTRODUCTION Role of
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Page 59 and 60: 2.1 - MATERIALS 2.1.1 BUFFERS AND R
Page 61 and 62: CHAPTER 2 ‐ METHODS Role of Th1 a
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GM‐CSF Granzyme A Granzyme B IFN
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CHAPTER 2 ‐ METHODS Role of Th1 a
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CHAPTER 3 IN VIVO ANALYSIS OF TH1 A
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Our experiments make several points
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Additionally, IL‐4 and IL‐5 wer
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3.4a, b). In addition, we consisten
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The CNS infiltrating mononuclear ce
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Histology and immunohistochemistry
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and brain stem and cerebellum more
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CHAPTER 3 - IN VIVO ANALYSIS OF TH1
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CHAPTER 4 EVALUATION OF CYTOTOXIC P
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4.1 - SUMMARY CHAPTER 4 - EVALUATIO
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CHAPTER 4 - EVALUATION OF CYTOTOXIC
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CHAPTER 5 MICROARRAY ANALYSIS OF T
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5.1 ‐ SUMMARY CHAPTER 5 -MICROARR
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CHAPTER 5 -MICROARRAY ANALYSIS OF T
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CHAPTER 6 GENERAL DISCUSSION AND MA
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CHAPTER 6 - GENERAL DISCUSSION AND
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CHAPTER 6 - GENERAL DISCUSSION AND
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REFERENCE LIST Abromson‐Leeman S,
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REFERENCE LIST Role of Th1 and Th17
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NOTES
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NOTES Role of Th1 and Th17 CD4 + T
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NOTES Role of Th1 and Th17 CD4 + T
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