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PROGRAMME
MAP<br />
EBC DEFINITION<br />
EBC is <strong>the</strong> scientifi c and technical arm of The Brewers of Europe<br />
EBC VISION<br />
Leadership through science and technology<br />
EBC MISSION STATEMENT<br />
To facilitate knowledge creation and transfer, and collaboration, between industrial and academic organisations,<br />
<strong>for</strong> <strong>the</strong> benefi t of <strong>the</strong> brewing sector, consumers and <strong>the</strong> community<br />
programme as of 22 April 2013, subject to change<br />
2<br />
Copyright MDI
INDEX<br />
The Brewers of Europe and EBC ............................................................................................................................. 04<br />
Committees ............................................................................................................................................................. 05<br />
Welcome by <strong>the</strong> EBC President ................................................................................................................................ 07<br />
Social Programme / Technical Tours ....................................................................................................................... 08<br />
General In<strong>for</strong>mation ................................................................................................................................................ 10<br />
Some Suggestions <strong>for</strong> … .......................................................................................................................................... 11<br />
Scientifi c Programme .............................................................................................................................................. 12<br />
Poster List / Poster Session ..................................................................................................................................... 18<br />
Lecture Abstracts .................................................................................................................................................... 24<br />
Poster Abstracts ...................................................................................................................................................... 44<br />
Guide to Beers and Breweries ................................................................................................................................ 82<br />
List of Exhibitors and Sponsors .............................................................................................................................. 84<br />
Exhibition Plan ......................................................................................................................................................... 85<br />
Exhibitor / Sponsor Guide ......................................................................................................................................... 87<br />
Evening Programme ................................................................................................................................................ 95<br />
Congress App ........................................................................................................................................................... 95<br />
3 34 TH EBC CONGRESS · LUXEMBOURG 26 – 30 MAY 2013
THE EUROPEAN BREWERY<br />
CONVENTION (EBC) IN 2013<br />
At <strong>the</strong> end of 2007, EBC merged with The Brewers of Europe, <strong>the</strong><br />
confederation <strong>for</strong> <strong>the</strong> brewing sector in Europe. EBC defi nes itself as<br />
<strong>the</strong> scientifi c and technical arm of The Brewers of Europe. EBC is<br />
responsible <strong>for</strong> <strong>the</strong> congress, <strong>the</strong> committees and groups, technical<br />
symposia and <strong>for</strong> its own budget within <strong>the</strong> organisational framework<br />
of The Brewers of Europe. For more on EBC’s history and activities log<br />
on to http://en.wikipedia.org/wiki/<strong>European</strong>_<strong>Brewery</strong>_Convention<br />
After <strong>the</strong> congress 2009 in Hamburg, EBC embarked on a strategy<br />
review. In short, <strong>the</strong> three pillars of EBC’s existence under <strong>the</strong> roof of<br />
The Brewers of Europe may be summarised as follows:<br />
To plan and execute world-class EBC congresses and symposia<br />
To facilitate knowledge creation and transfer through effective<br />
structures and governance<br />
To grow <strong>the</strong> impact of The Brewers of Europe through leveraging<br />
of EBC<br />
The Analysis Committee defi nes itself precisely in terms of <strong>the</strong> compendium<br />
of laboratory methods, known as Analytica. Methods are<br />
updated, revised, archived or newly drafted according to <strong>the</strong> needs<br />
of science in industry. Recently, <strong>the</strong> committee has started meeting<br />
again regularly; time was required in <strong>the</strong> interim to set out clear<br />
tasks and goals translating into modern methods needed by brewers<br />
globally.<br />
The Brewing Science Group hosted ano<strong>the</strong>r very successful meeting<br />
in Copenhagen, Denmark, immediately after an equally successful<br />
and well-attended EBC “From Chiller to Filler” Symposium in September<br />
2012. An unprecedented number of eight full professors of<br />
brewing science attended this event hosted courtesy of Carlsberg,<br />
toge<strong>the</strong>r with <strong>the</strong> Scandinavian School of Brewing. Membership<br />
numbers of <strong>the</strong> BSG are healthy, on <strong>the</strong> increase and have surpassed<br />
<strong>the</strong> mark of 60.<br />
The <strong>European</strong> Barley Variety Network, co-founded with our sister<br />
organisation Euromalt, is engaged in a review exercise on resourcing<br />
this important service to <strong>the</strong> beer production chain. More developments<br />
are expected <strong>for</strong> after <strong>the</strong> EBC congress.<br />
Strategical direction: Over and above its technical services, EBC has<br />
been proactive in shaping <strong>the</strong> future structure of The Brewers of Europe<br />
via <strong>the</strong> (Agility Strategy) emulated by both organisations.<br />
With <strong>the</strong> <strong>34th</strong> EBC congress in Luxembourg, <strong>the</strong> relationship between<br />
<strong>the</strong> two organisations has been fur<strong>the</strong>r cemented by linking<br />
<strong>the</strong> venue and timing of <strong>the</strong> congress with <strong>the</strong> statutory meetings of<br />
The Brewers of Europe.<br />
Membership: The one-envelope principle governing joint membership<br />
of EBC and The Brewers of Europe has now been fully harmonised:<br />
Croatia (a country that had been a member of EBC since 1999) joined<br />
The Brewers in 2011. Slovenia applied to <strong>for</strong>mally join <strong>the</strong> family only in<br />
January this year. They had been members of EBC since 1995.<br />
4<br />
COMPOSITION OF THE EBC<br />
EXECUTIVE COMMITTEE 2013<br />
The current number of members of <strong>the</strong> EBC Executive Committee<br />
stands at 12, according to <strong>the</strong> governance structure outlined below.<br />
Members are nominated by <strong>the</strong> national trade associations and major<br />
brewers. Final approval of its composition rests with <strong>the</strong> <strong>the</strong> EBC<br />
President (and indirectly with <strong>the</strong> Board of The Brewers of Europe).<br />
The EBC President acts as <strong>the</strong> chairman of <strong>the</strong> committee, assisted<br />
by John M. Brauer, <strong>the</strong> EBC Executive Offi cer.<br />
EBC Executive Officer:<br />
John Brauer<br />
The EBC Executive Committee<br />
National Trade Association<br />
representation:<br />
WEST: Hedwig Neven<br />
(Duvel-Moortgat, BE)<br />
SOUTH: Benet Fité Luis (Mahou-San Miguel, ES)<br />
NORTH: Bo Ranta (Sinebrychoff, FI)<br />
CENTRAL: Martin Ketterer<br />
(Brau. Schützengarten, CH)<br />
EAST: currently vacant due to retirement of<br />
Jan Vesely (Czech Beer & Malt Assoc., CZ)<br />
EBC President<br />
Stefan Lustig<br />
(Brau Holding Internat., DE)<br />
The Brewers<br />
of<br />
Europe<br />
BOARD<br />
Regulatory Affairs Director:<br />
Anna-Maria De Smet<br />
Major and Non-Major Brewer<br />
representation:<br />
HEINEKEN: Willem van Waesberghe<br />
CARLSBERG: Stefan Kreisz (Vice-President)<br />
AB-INBEV: Carsten Eger<br />
SABMILLER: Simon Wade<br />
Guinness Diageo:<br />
Gearoid Cahill<br />
Wells & Young: Jim Robertson<br />
THE EBC LOCAL ORGANISING<br />
COMMITTEE FOR THE LUXEMBOURG<br />
CONGRESS<br />
Chairman: Georges M. Lentz supported by Thierry Krombach,<br />
Brasserie Nationale SA (Boffering), Bascharage (Luxembourg)<br />
Allied Traders Representation: André Thix supported by Nathalie<br />
Proess, Micro-Matic, Troisvierges (Luxembourg)<br />
The PCO management team, led by Astrid Remnitz, INTERPLAN AG,<br />
Hamburg, and Oliver Heinke, Munich (Germany)<br />
The scientifi c and technical contributions <strong>for</strong> <strong>the</strong> EBC congress programme<br />
refl ect <strong>the</strong> collective inputs of members of <strong>the</strong> selection<br />
committee. Members of this committee are drawn from <strong>the</strong> Executive<br />
Committee, <strong>the</strong> chairmen of <strong>the</strong> EBC Analysis Committee, <strong>the</strong><br />
<strong>European</strong> Barley Variety Network, and of <strong>the</strong> EBC Brewing Science<br />
Group.
THE BREWERS OF EUROPE<br />
EVENTS FOR 2013<br />
Faces of Brewing<br />
An exciting photography contest on beer and brewing organised by<br />
The Brewers of Europe with high quality photography equipment<br />
to be won. This third edition has a focus on beer in <strong>the</strong> hospitality<br />
trade: bars, pubs, cafés, beer gardens, beer halls, festivals etc.<br />
Photography, beer and brewing enthusiasts are encouraged to enter.<br />
Nominees will also have <strong>the</strong>ir photographs displayed at a Brussels<br />
exhibition and shown at Beer Serves Europe 2013. The competition<br />
runs throughout <strong>the</strong> summer and more details are available on<br />
www.facesofbrewing.eu<br />
The Brewers of Europe’s mission is: “To equip Europe’s brewers with <strong>the</strong><br />
tools to freely, cost-effectively and responsibly brew and market beer.”<br />
For more in<strong>for</strong>mation, log onto www.brewersofeurope.org.<br />
Beer Serves Europe IV<br />
Gala evening taking place in Brussels on 4 December 2013. This<br />
annual conference and food-pairing beer reception will once again<br />
tackle <strong>the</strong> key issues facing brewers across Europe, looking in detail<br />
at <strong>the</strong> important role played by brewing in Europe’s society and economy.<br />
Registrations will open later in <strong>the</strong> year as <strong>the</strong> full line-up <strong>for</strong><br />
<strong>the</strong> panel discussion and debate unfolds. Keep up to date with <strong>the</strong><br />
latest developments at www.beerserveseurope.eu<br />
EBC EVENTS 2014 & 2015<br />
EBC Symposium 2014<br />
7 – 9 September 2014<br />
Venue: Vienna (Austria). Provisional topic allocation: Flavour and<br />
Sensory of Beer and Flavoured Beers<br />
EBC Brewing Science Group 10th Technical Meeting<br />
10 – 11 September 2014<br />
Venue: Vienna (Austria)<br />
35th EBC Congress<br />
24 – 28 May 2015<br />
to be held at <strong>the</strong> Centro de Congressos da Alfândega do Porto, Portugal.<br />
See also page 81 of this programme.<br />
The seat of EBC at The Brewers of Europe House in Rue Caroly in Brussels<br />
5 34 TH EBC CONGRESS · LUXEMBOURG 26 – 30 MAY 2013
See you in Luxembourg<br />
at <strong>the</strong> EBC Congress!<br />
Fachverlag Hans Carl GmbH<br />
Andernacher Straße 33 a<br />
90411 Nürnberg<br />
Deutschland / Germany<br />
Access to knowledge - 24 hours / 7 days<br />
Print - Newsletter - Online<br />
Visit us at our stand # 46 and get<br />
more and detailed in<strong>for</strong>mation about<br />
“Analytica-EBC online”.<br />
We are looking <strong>for</strong>ward to seeing you.<br />
Tel.: ++49 (0)911 9 52 85 - 0<br />
Fax: ++49 (0)911 9 52 85 - 81 42<br />
E-Mail: info@hanscarl.com<br />
www.hanscarl.com
WELCOME BY THE EBC PRESIDENT<br />
On behalf of <strong>the</strong> <strong>European</strong> <strong>Brewery</strong> Convention it is a pleasure to welcome you to visit Luxembourg<br />
<strong>for</strong> <strong>the</strong> 34 th EBC congress. Our congress will be held at <strong>the</strong> Lux Congrès facilities overlooking <strong>the</strong><br />
historical city of Luxembourg on <strong>the</strong> plateau de Kirchberg, surrounded by <strong>the</strong> <strong>European</strong> Court of<br />
Justice and o<strong>the</strong>r important EU institutions. Historic decisions concerning <strong>the</strong> <strong>European</strong> Union have<br />
been taken in <strong>the</strong>se venues which provides an excellent setting <strong>for</strong> our technical, technological and<br />
scientifi c lectures and presentations. In light of our increasingly integrated part within The Brewers<br />
of Europe, it is a venue that refl ects both our status and relationship within <strong>the</strong> <strong>European</strong> framework<br />
of industry associations.<br />
Hardly any o<strong>the</strong>r <strong>European</strong> capital city serves up such an impressive array of contrasts as Luxembourg.<br />
In <strong>the</strong> course of its history, spanning more than a thousand years, <strong>the</strong> city has grown from<br />
“Lucilinburhuc”, <strong>the</strong> seat of Siegfried, <strong>the</strong> fi rst Count of Luxembourg, to <strong>the</strong> prosperous metropolis<br />
it is today. In between lie centuries of turbulent history, refl ected in <strong>the</strong> city’s silhouette that towers<br />
above <strong>the</strong> impressive remains of <strong>the</strong> historic <strong>for</strong>tress. Its population is polyglot, cosmopolitan and<br />
most welcoming.<br />
I look <strong>for</strong>ward to meet scientists, brewers, maltsters and colleagues from <strong>the</strong> supplying industry<br />
and have a fruitful exchange of experiences, knowledge and passion <strong>for</strong> our product.<br />
With my best wishes <strong>for</strong> a superb congress,<br />
See you,<br />
Bis bald,<br />
A bientôt,<br />
Nos vemos,<br />
Dr. Stefan Lustig<br />
COO Brau Holding International GmbH & Co. KGaA<br />
CONGRESS VENUE<br />
Nouveau Centre de Conférences<br />
4 Place de l’ Europe<br />
L-1499 Luxembourg<br />
www.luxcongress.de<br />
Source Luxemburg Congrès<br />
7 34 TH EBC CONGRESS · LUXEMBOURG 26 – 30 MAY 2013
SOCIAL PROGRAMME<br />
SUNDAY, 26 MAY 2013, 13:30 – 15:30 h<br />
City-tour on foot<br />
Start: Place de la Constitution (Golden Lady) in Luxembourg-City<br />
Price per person: € 10.00<br />
Incl. guide<br />
SUNDAY, 26 MAY 2013, 15:00 – 17:00 h<br />
City-tour by bus<br />
Start: NCCK – Nouveau Centre de Conférences Kirchberg<br />
Price per person: € 10.00<br />
Incl. bus, guide<br />
TECHNICAL TOURS ON THURSDAY, 30 MAY 2013<br />
All tours start and fi nish at NCCK - Nouveau Centre de Conférences Kirchberg.<br />
TOUR 1: Bitburger Braugruppe GmbH<br />
in Bitburg / Germany<br />
Price per person: € 55.00 (including lunch package)<br />
8:30 - 14:00 h (arrival at Luxembourg Airport: approx. 13:30 h)<br />
(arrival at NCCK: approx. 13:45 h)<br />
(arrival at Main Station: approx. 14:00 h)<br />
TOUR 2A: Brasserie d‘Orval<br />
in Florenville / Belgium<br />
Price per person: € 60.00 (including lunch package)<br />
8:00 - 14:00 h (arrival at Luxembourg Airport: approx. 13:30 h)<br />
(arrival at NCCK: approx. 13:45 h)<br />
(arrival at Main Station: approx. 14:00 h)<br />
TOUR 2B: Brasserie d‘Orval<br />
in Florenville / Belgium<br />
Price per person: € 45.00<br />
13:00 - 19:00 h (arrival at Luxembourg Airport: approx. 18:30 h)<br />
(arrival at NCCK: approx. 18:45 h)<br />
(arrival at Main Station: approx. 19:00 h)<br />
TOUR 3: Caves Bernard-Massard<br />
in Grevenmacher / Luxembourg<br />
Price per person: € 25.00<br />
8:45 - 12:00 h (arrival at Luxembourg Airport: approx. 11:30 h)<br />
(arrival at NCCK: approx. 11:45 h)<br />
(arrival at Main Station: approx. 12:00 h)<br />
TOUR 4A: Brasserie Nationale<br />
in Bascharage (Nidderkäerjeng) / Luxembourg<br />
Price per person: € 25.00<br />
8:15 - 12:00 h (arrival at Luxembourg Airport: approx. 11:30 h)<br />
(arrival at NCCK: approx. 11:45 h)<br />
(arrival at Main Station: approx. 12:00 h)<br />
8<br />
MONDAY, 27 MAY 2013, 09:00 – 18:00 h<br />
Three-Country-Tour: Luxembourg – Metz – Trier<br />
Start: NCCK – Nouveau Centre de Conférences Kirchberg<br />
Price per person: Incl. in partner’s registration fee<br />
For normal registered participants price per person € 80.00<br />
Incl. bus, guide, lunch<br />
TOUR 4B: Brasserie Nationale<br />
in Bascharage (Nidderkäerjeng) / Luxembourg<br />
Price per person: € 25.00<br />
13:45 - 17:30 h (arrival at Luxembourg Airport: approx. 17:00 h)<br />
(arrival at NCCK: approx. 17:15 h)<br />
(arrival at Main Station: approx. 17:30 h)<br />
TOUR 5: Malteurop<br />
in Metz / France<br />
Price per person: € 55.00 (including lunch package)<br />
8:30 - 14:00 h (arrival at Luxembourg Airport: approx. 13:30 h)<br />
(arrival at NCCK: approx. 13:45 h)<br />
(arrival at Main Station: approx. 14:00 h)<br />
TOUR 6A: IFBM-Qualtech<br />
in France<br />
Price per person: € 55.00 (including lunch)<br />
8:00 - 17:00 h (arrival at Luxembourg Airport: approx. 16:30 h)<br />
(arrival at NCCK: approx. 16:45 h)<br />
(arrival at Main Station: approx. 17:00 h)<br />
TOUR 6B: Champigneulles & IFBM-Qualtech<br />
in France<br />
Price per person: € 55.00 (including lunch)<br />
8:00 - 17:00 h (arrival at Luxembourg Airport: approx. 16:30 h)<br />
(arrival at NCCK: approx. 16:45 h)<br />
(arrival at Main Station: approx. 17:00 h)
Always <strong>the</strong> same<br />
taste – worldwide.
GENERAL INFORMATION<br />
REGISTRATION DESK<br />
The registration desk in <strong>the</strong> NCCK - Noveau Centre de Conférences<br />
Kirchberg will be open as follows:<br />
Sunday, 26 May 2013: 13:00 – 20:00<br />
Monday, 27 May 2013: 07:30 – 18:00<br />
Tuesday, 28 May 2013: 08:00 – 18:00<br />
Wednesday, 29 May 2013: 08:00 – 16:30<br />
EXHIBITION<br />
The exhibition will be located in <strong>the</strong> foyer of <strong>the</strong> lecture halls. The coffee<br />
breaks and lunch breaks will also take place in this area. Detailed<br />
in<strong>for</strong>mation, including an interactive fl oor plan, can be found on <strong>the</strong><br />
congress website.<br />
The exhibition opening hours are currently under review following<br />
suggestions from <strong>the</strong> technical suppliers and exhibitor:<br />
Sunday, 26 May 2013: 18:00 – 23:00<br />
Monday, 27 May 2013: 10:00 – 16:30<br />
Tuesday, 28 May 2013: 12:00 – 20:00<br />
(Micro-Matic Congress Sundowner 16:30 – 20:00)<br />
Wednesday, 29 May 2013: 10:00 – 16:30<br />
CONGRESS LANGUAGE<br />
The congress language is English. Simultaneous translations will<br />
not be provided.<br />
DRESS CODE<br />
The dress code is smart casual <strong>for</strong> <strong>the</strong> evening events.<br />
INSURANCE<br />
Registration fees do not include insurance of any kind. It is strongly<br />
recommended that all delegates take out <strong>the</strong>ir own travel and medical<br />
insurance prior to coming to <strong>the</strong> congress. The organisers will<br />
not take any responsibility <strong>for</strong> any participant failing to insure.<br />
10<br />
PASSPORT AND VISA REQUIREMENTS<br />
EU residents and visitors from US, Canada, Australia and New Zealand<br />
do not need visas <strong>for</strong> stays up to 3 months; only a valid passport<br />
is required. All o<strong>the</strong>r visitors are requested to check <strong>the</strong> specifi c travel<br />
requirements with <strong>the</strong>ir own diplomatic mission.<br />
NOTICE BOARD<br />
Congress announcements and messages <strong>for</strong> delegates will be posted<br />
on a notice board near <strong>the</strong> registration desk. No announcements<br />
will be made during <strong>the</strong> sessions unless absolutely necessary.<br />
Please check <strong>the</strong> board regularly.<br />
LUNCH / REFRESHMENT<br />
Lunches will be served to all delegates from Monday through Wednesday<br />
in <strong>the</strong> exhibition area. Lunches <strong>for</strong> partners are not <strong>for</strong>eseen;<br />
<strong>the</strong>se are included in <strong>the</strong> tours w<strong>here</strong> indicated. By courtesy of <strong>the</strong><br />
hosts, beer and mineral water are available throughout <strong>the</strong> congress<br />
in <strong>the</strong> exhibition area as well.<br />
POSTERS<br />
Please note that <strong>the</strong> poster presentations will not taking place during<br />
lunch this year. T<strong>here</strong> will be a special time only <strong>for</strong> <strong>the</strong> poster<br />
presentations on Tuesday, 28 May 2013 at 5.00 pm. During this so<br />
called “Poster Session”, <strong>the</strong> poster presenters will be available at<br />
<strong>the</strong>ir poster <strong>for</strong> questions and discussions.<br />
WLAN / WIFI<br />
WLAN / WiFi is available <strong>for</strong> all participants throughout <strong>the</strong> NCCK.<br />
Network: EBC Congress<br />
Password: EBC13LUX
SOME SUGGESTIONS FOR …<br />
Restaurants in Luxembourg<br />
Restaurant „Am Tiirmchen“<br />
The restaurant „Am Tiirmchen” serves French and Luxembourgish<br />
dishes from Monday til Saturday in an medieval building in <strong>the</strong> city<br />
of Luxembourg.<br />
32 rue de l’eau, 1449 Luxembourg<br />
phone: +352 26 27 07 33<br />
www.amtiirmschen.lu<br />
Wainstuff-Restaurnt „Kniddelkinnek“<br />
Enjoy typical Luxembourgish menus in <strong>the</strong> Wainstuff-Restaurant<br />
„Kniddelkinnek“.<br />
4 Rue de la Loge, 1945 Luxembourg<br />
phone: +352 27 47 80 59<br />
www.Kniddelkinnek.lu<br />
Brasserie Guillaume<br />
The Brasserie Guillaume is well known <strong>for</strong> excellent fi sh menus<br />
located in <strong>the</strong> opposite of <strong>the</strong> town hall.<br />
12 – 14 Place de Guillaume II, 1648 Luxembourg<br />
phone: +352 26 20 20 20<br />
www.brasserieguillaume.lu<br />
Restaurante Notaro<br />
Italian food is served in <strong>the</strong> Restaurante Notaro.<br />
149 Rue de la Tour Jacob, 1831 Luxembourg<br />
phone: +352 42 30 70<br />
www.notaro.lu<br />
Bars und Pubs<br />
Palais<br />
Centrally located in <strong>the</strong> heart of Luxembourg, <strong>the</strong> Palais has set<br />
<strong>the</strong> standard of excellence that many new venues strive to emulate.<br />
Within its stylized ambience, you can ei<strong>the</strong>r relax with friends in an<br />
environment of sophisticated privacy or blend in with <strong>the</strong> crowd dancing<br />
to soundscapes created by Luxembourg‘s leading DJs.<br />
13 rue de Marché-aux-Herbes, 1728 Luxembourg<br />
phone: +352 26 26 24 90<br />
www.lepalais.lu<br />
Go Ten bar & café<br />
Starting from it‘s Japan-inspired design through a personalized service<br />
and products not to be found regularly in Luxembourg town, everything<br />
is set to welcome you in a cosy and relaxing atmosp<strong>here</strong> in<br />
<strong>the</strong> heart of <strong>the</strong> city.<br />
10 rue de Marché-aux-Herbes, 1728 Luxembourg<br />
phone: +352 26 20 36 52<br />
www.gotencafe.com<br />
11 34 TH EBC CONGRESS · LUXEMBOURG 26 – 30 MAY 2013
SCIENTIFIC PROGRAMME<br />
Sunday, 26 May 2013<br />
13.00 - 20.00 Registration<br />
18.00 - 23.00 Opening Ceremony in <strong>the</strong> Hémicycle and Welcome Party in <strong>the</strong> exhibition area in <strong>the</strong> NCCK in <strong>the</strong> presence of<br />
His Royal Highness Grand Duke Henri I. Attending dignitaries include Dr. Stefan Lustig, <strong>the</strong> current EBC President,<br />
Mr. Christian von der Heide (immediate past president) and Prof. Dr. Ludwig Narziss, honorary member of<br />
<strong>the</strong> EBC. “Brewers <strong>for</strong> Brass” is an ad-hoc ensemble composed of members active in <strong>the</strong> brewing sector under<br />
<strong>the</strong> baton of Dr. Karl-Ulrich Heyse. The audience is requested to be seated by 17.50 hrs.<br />
Monday, 27 May 2013 Salle Hémicycle PLENARY SESSION<br />
07.30 - 18.00 Registration<br />
10.00 - 16.30 Exhibition<br />
OPENING SESSION<br />
Chair: Stefan Lustig, EBC President, Brau Holding Internat. GmbH & Co. KGaA, Germany<br />
08.30 - 09.00 Opening address by <strong>the</strong> CEO of <strong>the</strong> Brasserie Nationale, Georges M. Lentz jr.:<br />
An overview of beer brewing in Luxembourg.<br />
09.00 - 09.30 L1 Key-note 1: Brewing’s Got Talent – The Impact of Brewing on Science<br />
Smart K., SABMiller plc, United Kingdom<br />
09.30 - 10.00 L2 Key-note 2: EBC and EBCU: Science and <strong>the</strong> beer consumer. A win-win relationship?<br />
Reuchlin, H., Chairman of <strong>the</strong> <strong>European</strong> Beer Consumers Union (EBCU), United Kingdom<br />
10.00 - 10.30 COFFEE BREAK<br />
GENERAL REVIEW PAPERS<br />
Chair: Stefan Kreisz, Carlsberg A/S, Denmark<br />
10.30 - 11.00 L3 The environmental per<strong>for</strong>mance of <strong>the</strong> <strong>European</strong> brewing sector.<br />
Koop, J., KWA Bedrijfsadviseurs BV, The Ne<strong>the</strong>rlands<br />
11.00 - 11.30 L4 Worldwide energy and water effi ciency benchmark.<br />
Juriaanse, T., Dutch Brewers Association, The Ne<strong>the</strong>rlands<br />
11.30 - 12.00 L5 Advances in hop aroma research - how much do we know today?<br />
Schönberger, C., Barth Innovations, Germany<br />
12.00 - 12.30 L6 Craft brewing: The new global <strong>for</strong>ce and its implication <strong>for</strong> <strong>the</strong> brewing industry.<br />
Nelson, L., Advantage Publishing Ltd, United Kingdom<br />
12.30 - 14.00 LUNCH & EXHIBITION<br />
Monday, 27 May 2013 Salle Hémicycle PARALLEL SESSION 1<br />
BREWING & ENVIRONMENT<br />
Chair: Gert De Rouck, KAHO St.-Lieven / KUL Association, Belgium<br />
14.00 - 14.30 L7 Energy management isn‘t about data, it‘s about translating in<strong>for</strong>mation into action.<br />
Wilson, N., Scanenergi Solutions A/S, Denmark<br />
14.30 - 15.00 L8 A novel water quality management strategy in breweries.<br />
Blomenhofer, V., University of Erlangen-Nuremberg, Germany<br />
15.00 - 15.30 L9 Optimising <strong>the</strong> pre-treatment of Brewers‘ Grains to liberate sugars <strong>for</strong> bioethanol production.<br />
Cook, D., University of Nottingham, United Kingdom<br />
15.30 - 16.00 L10 Is it time to brew without boiling?<br />
Feilner, R., Krones AG, Germany<br />
16.00 - 16.30 COFFEE BREAK<br />
16.30 - 17.00 L11 Bio-based alternatives <strong>for</strong> polyester in packaging.<br />
Harlin, A., VTT, Finland<br />
17.00 - 17.30 L12 Energy consumption and possibilities to reduce heat impact during hop kilning.<br />
Hofmann, R., Versuchs- und Lehranstalt für Brauerei in Berlin (VLB) e.V., Germany<br />
12
Monday, 27 May 2013 Salle C PARALLEL SESSION 2<br />
BEER & FLAVOUR I<br />
Chair: Frank-Jürgen Methner, TU Berlin, Germany<br />
14.00 - 14.30 L13 Principal components analysis of aroma compounds in beer.<br />
Irie, T., Asahi Breweries Ltd., Japan<br />
14.30 - 15.00 L14 A new stable isotope dilution approach <strong>for</strong> <strong>the</strong> sensitive quantitation of 3-methyl-2-buten-1-thiol (MBT) and its<br />
application to study sunstruck-fl avour development in beer and its application to study sunstruck-fl avour development<br />
in beer.<br />
Stingl, S., German Research Centre <strong>for</strong> Food Chemistry, Germany<br />
15.00 - 15.30 L15 A kinetic framework <strong>for</strong> <strong>the</strong> modeling of beer fl avour instability.<br />
Hughes, P., International Centre <strong>for</strong> Brewing and Distilling, United Kingdom<br />
15.30 - 16.00 L16 Amino acid oxidation - an unrevealed pathway leading to <strong>the</strong> <strong>for</strong>mation of staling aldehydes in bottled beer?<br />
Wietstock, P., Technische Universität Berlin, Germany<br />
16.00 - 16.30 COFFEE BREAK<br />
16.30 - 17.00 L17 Detoxifi cation of off-fl avor unsaturated carbonyl compounds: Purifi cation and characterization of enone reductases<br />
from yeast with new catalytic activities.<br />
Garbe, L-A., TUB / VLB, Germany<br />
17.00 - 17.30 L18 Quantitation of desirable aroma-active and undesirable toxicologically relevant vinyl aromatic compounds in<br />
wheat beer.<br />
Langos, D., Deutsche Forschungsanstalt für Lebensmittelchemie, Germany<br />
GlobalMalt Pub Night<br />
13 34 TH EBC CONGRESS · LUXEMBOURG 26 – 30 MAY 2013
Tuesday, 28 May 2013 Salle Hémicycle PARALLEL SESSION 1<br />
08.00 - 18.00 Registration<br />
12.30 - 20.00 Exhibition<br />
GUSHING<br />
Chair: Hedwig Neven, Brouwerij Duvel-Moortgat, Belgium<br />
08.30 - 09.00 L19 Infl uence of selected hop and beer substances on gushing.<br />
Titze, J., National University of Ireland, Ireland<br />
09.00 - 09.30 L20 Hop Lipids - A comprehensive overview with regard to beer foam, fl avour (in)stability and gushing.<br />
Rettberg, N., VLB Berlin / TU Berlin, Germany<br />
09.30 - 10.00 L21 Tool <strong>for</strong> predicting <strong>the</strong> risk of early gushing from harvest to come.<br />
Boivin, P., IFBM, France<br />
10.00 - 10.30 COFFEE BREAK<br />
MALT PRODUCTION & QUALITY<br />
Chair: Dietrich Mönch, GlobalMalt, Germany<br />
10.30 - 11.00 L22 Malting at low moisture to save energy.<br />
Schmitt, M., IFBM, France<br />
11.00 - 11.30 L23 The infl uence of <strong>the</strong> kilning process on <strong>the</strong> gelatinization temperature of barley malt.<br />
Rittenauer, M., TUM Weihenstephan, Germany<br />
11.30 - 12.00 L24 A novel approach to malt fl avour characterization.<br />
Voigt, J., University of Applied Sciences Trier, Germany<br />
12.00 - 12.30 L25 Iso<strong>the</strong>rmal and non-iso<strong>the</strong>rmal decomposition kinetics of s-methylmethionin.<br />
Huang, Y., Technische Universität München, Germany<br />
12.30 - 14.00 LUNCH & EXHIBITION<br />
13.00 - 13.45 Business Meeting of <strong>the</strong> EBC Brewing Science Group (Note: members only) – Briefi ng Room IT<br />
RAW MATERIALS MIXED SESSION<br />
Chair: Jean Titze, National University of Ireland, Ireland<br />
14.00 - 14.30 L33 MALDI-TOF MS imaging of barley seeds during malting.<br />
Gorzolka, K., Bielefeld University, Germany<br />
14.30 - 15.00 L34 Brewing properties of new German hop varieties.<br />
Forster, A., HVG e.G., Germany<br />
15.00 - 15.30 L35 Fusarium and mycotoxins in barley from monitoring to risk management in France.<br />
Froment, A., Syngenta, France<br />
15.30 - 16.00 L36 Development of a rapid and sensitive multi-residue method to determine pesticides in hops and<br />
hop products.<br />
Biendl, M., Hopsteiner HHV GmbH, Germany<br />
16.00 - 16.30 L37 Effi cient processing methods <strong>for</strong> increasing yield when using hop products <strong>for</strong> wort production.<br />
Scheller, L., GEA <strong>Brewery</strong> Systems GmbH, Germany<br />
16.30 - 20.00 Micro-Matic Congress Sundowner in <strong>the</strong> exhibition and poster areas<br />
17.00 - 17.30 Poster Session<br />
Poster presenters to be available <strong>for</strong> questions / discussions.<br />
14
Tuesday, 28 May 2013 Salle C PARALLEL SESSION 2<br />
08.00 - 18.00 Registration<br />
12.30 - 20.00 Exhibition<br />
BEER & FLAVOUR II<br />
Chair: Caroline Walker, Campden-BRI, United Kingdom<br />
08.30 - 09.00 L26 Fermentable and non-fermentable carbohydrates addition during brewing - effects on palate fullness, oxidative<br />
processes and <strong>for</strong>mation of specifi c aging compounds.<br />
Brandt, N., Technische Universität Berlin, Germany<br />
09.00 - 09.30 L27 Control of cereal fl avor of beer by changing <strong>the</strong> pH and amino acid and reducing sugar composition in wort<br />
during boiling.<br />
Inaba, S., Asahi Breweries Ltd., Japan<br />
09.30 - 10.00 L28 Infl uence of matrix composition on body and mouthfeel - maltodextrins <strong>the</strong> body builders!<br />
Gastl, M., Technische Universität München, Germany<br />
10.00 - 10.30 COFFEE BREAK<br />
YEAST & FERMENTATION<br />
Chair: Ka<strong>the</strong>rine Smart, SABMiller plc, United Kingdom<br />
10.30 - 11.00 L29 RNAseq analysis of Saccharomyces cerevisiae‘s response to contamination by Lactobacillus plantarum.<br />
Phister, T., University of Nottingham, United Kingdom<br />
11.00 - 11.30 L30 The effect of successive use of lager yeast starter culture on sugar uptake dynamics from wort.<br />
Kočar, N., Pivovarna Laško d.d., Slovenia<br />
11.30 - 12.00 L31 A comparison of fermentation by-products to determine beer maturation regardless of style and processing<br />
parameters.<br />
Wellhoener, U., The Boston Beer Company, United States<br />
12.00 - 12.30 L32 Use and maintenance of synchronous yeast cultures.<br />
Sheppard, J., North Carolina State University, United States<br />
12.30 - 14.00 LUNCH & EXHIBITION<br />
13.00 - 13.45 Business Meeting of <strong>the</strong> EBC Brewing Science Group (Note: members only) – Briefi ng Room IT<br />
FILTRATION & PACKAGING<br />
Chair: Roland Pahl, VLB Berlin, Germany<br />
14.00 - 14.30 L38 Classifi cation of renewable fi lter aids - an opportunity to control <strong>the</strong> permeability of <strong>the</strong> fi lter cake.<br />
Scheidel, A., TU München, Germany<br />
14.30 - 15.00 L39 Validity of <strong>the</strong> laboratory fi ltration as a prediction of <strong>the</strong> fi lterability of beer.<br />
Kupetz, M., TUM Weihenstephan, Germany<br />
15.00 - 15.30 L40 Permeation and migration behavior of polyethylene terephthalate polymer, PET/PA polymer blends and SiOx<br />
coated PET bottles.<br />
Sängerlaub, S., Fraunhofer Institute <strong>for</strong> Process Engineering and Packaging IVV, Germany<br />
15.30 - 16.00 L41 Establishment of a new beer canning process based on dew-point temperature fi lling technology.<br />
Otake, I., Asahi Breweries, Ltd., Japan<br />
16.00 - 16.30 L42 Time-Temperature Integrator <strong>for</strong> <strong>the</strong> fl ash pasteurization.<br />
Schneider, J., University of Applied Sciences Ostwestfalen-Lippe, Germany<br />
16.30 - 20.00 Micro-Matic Congress Sundowner in <strong>the</strong> exhibition and poster areas<br />
17.00 - 17.30 Poster Session<br />
Poster presenters to be available <strong>for</strong> questions / discussions.<br />
15 34 TH EBC CONGRESS · LUXEMBOURG 26 – 30 MAY 2013
Wednesday, 29 May 2013 Salle Hémicycle PARALLEL SESSION 1<br />
08.00 - 16.30 Registration<br />
10.00 - 16.30 Exhibition<br />
NEW PRODUCT DEVELOPMENT<br />
Chair: Steve Livens, British Beer & Pub Association, United Kingdom<br />
08.30 - 09.00 L43 Study of different rice varieties and malting parameters <strong>for</strong> <strong>the</strong> production of a saccharifying rice malt.<br />
Perretti, G., University of Perugia, Italy<br />
09.00 - 09.30 L44 Creating novel raw materials <strong>for</strong> beer production - development of LOX-less barley and <strong>the</strong> future strategy.<br />
Kuroda, H., Sapporo Breweries Ltd., Japan<br />
09.30 - 10.00 L45 New opportunities <strong>for</strong> high attenuated beers demonstrated by a <strong>the</strong>rmostable glycoamylase from<br />
Penicillium oxalicum.<br />
Eklöf, J., Novozymes A/S, Denmark<br />
10.00 - 10.30 COFFEE BREAK<br />
WORT PRODUCTION<br />
Chair: Tiago Monteiro Brandão, Unicer Bebidas SA, Portugal<br />
10.30 - 11.00 L46 Mashing with direct steam heating and without mechanical agitation.<br />
Kern, M., Technische Universität München, Germany<br />
11.00 - 11.30 L47 High mashing-off temperature and acidifi cation of sparging water extending fl avour stability of beer.<br />
De Rouck, G., KAHO Sint-Lieven / KUL Association, Belgium<br />
11.30 - 12.00 L48 Infl uence of starch sources and mashing procedures on <strong>the</strong> range of Molecular Weight Distribution (MWD)<br />
and <strong>the</strong> intensity of palate fullness of beer.<br />
Rübsam, H., Technische Universität München (TUM), Germany<br />
12.00 - 12.30 L49 Changes in protein profi le and enzymatic activity during mashing when using different grist compositions of<br />
barley and wheat malt and <strong>the</strong>ir infl uence to beer quality.<br />
Faltermaier, A. University College Cork, Ireland<br />
12.30 - 13.00 L50 Procedural considerations to <strong>the</strong> starch conversion during mashing.<br />
Henke, S., TU München, Germany<br />
13.00 - 14.30 LUNCH & EXHIBITION<br />
Wednesday, 29 May 2013 Salle Hémicycle PLENARY SESSION<br />
GENERALIST SESSION<br />
Chair: Gearoid Cahill, Guinness (Diageo), Ireland<br />
14.30 - 15.00 L59 Microbiological analysis of a shipwreck beer.<br />
Juvonen, R., VTT Technical Research, Finland<br />
15.00 - 15.30 L60 Total process optimization via KAIZEN-ASAHI way: Technological innovations spreading system.<br />
Ito, Y., Asahi Breweries Ltd., Japan<br />
15.30 - 16.00 L61 Bitter substance yield during <strong>the</strong> brewing process - Infl uencing factors and possibilities of recovery<br />
Methner, F.-J., Technische Universität Berlin, Germany<br />
16.00 - 16.30 COFFEE BREAK<br />
Chair: Stefan Lustig, EBC President, and John M. Brauer, EBC Executive Offi cer (The Brewers of Europe)<br />
16.30 - 18.00 Closing Session & Panel Discussion on “Role of research, development and technology in <strong>the</strong> brewing<br />
industry and expected deliverables” to be moderated by Larry Nelson (Brewers‘ Guardian)<br />
Invitation <strong>for</strong> <strong>the</strong> 35th EBC congress to be held from 24-28 May 2015 in Porto. The Secretary-General of <strong>the</strong><br />
Portuguese Brewers’ Association (ACPV), Francisco Girio, will introduce <strong>the</strong> host association and venue.<br />
Also see page 81.<br />
19.00 - 23.00 Congress Farewell Party at <strong>the</strong> Place Guillaume II in <strong>the</strong> city centre<br />
Offi cial opening by Mayor Xavier Bettel at 19.45 hrs<br />
16
Wednesday, 29 May 2013 Salle C PARALLEL SESSION 2<br />
08.00 - 16.30 Registration<br />
10.00 - 16.30 Exhibition<br />
BEER & FLAVOUR III<br />
Chair: Benet Fité Luis, Grupo Mahou San Miguel, Spain<br />
08.30 - 09.00 L51 Development of methods to measure real-time fl avour profi les.<br />
Parker, D., Campden BRI, United Kingdom<br />
09.00 - 09.30 L52 Relationships between <strong>the</strong> chemical-physical composition, <strong>the</strong> sensory descriptive analysis and consumer<br />
acceptance of German wheat beer .<br />
Schmelzle, A., Hochschule Geisenheim University, Germany<br />
09.30 - 10.00 L53 Comparison of two techniques <strong>for</strong> maturation of beer using oak.<br />
Poleto, L., University of São Paulo, Brazil<br />
10.00 - 10.30 COFFEE BREAK<br />
MICROBIOLOGY & BIOCHEMISTRY<br />
Chair: Stefan Kreisz, Carlsberg A/S, Denmark<br />
10.30 - 11.00 L54 Biogeography, population genomics and technol. properties of <strong>the</strong> lager-brewing ancestor, S. eubayanus.<br />
Libkind, D., INIBIOMA (CONICET-UNComahue), Argentina<br />
11.00 - 11.30 L55 A new maltose/maltotriose transporter (Saccharomyces eubayanus-type Agt1) in lager yeast and its<br />
relevance to fermentation per<strong>for</strong>mance.<br />
Vidgren, V., VTT Technical Research Centre of Finland, Finland<br />
11.30 - 12.00 L56 Development of <strong>the</strong> direct DNA extraction method <strong>for</strong> rapid detection of low concentrations of beer-spoilage<br />
lactic acid bacteria.<br />
Shimokawa, M., Asahi Breweries Ltd., Japan<br />
12.00 - 12.30 L57 Evaluation of biofi lm composition and maturation using realtime-PCR.<br />
Hutzler, M., TUM Weihenstephan, Germany<br />
12.30 - 13.00 L58 Diversity of xylanolytic bacteria and xylanase genes during industrial malting.<br />
Malfl iet, S., KAHO St.-Lieven, Belgium<br />
13.00 - 14.30 LUNCH & EXHIBITION<br />
PLENARY SESSION – see programme page 16<br />
Farewell Party in <strong>the</strong> heart of <strong>the</strong> city<br />
This year’s Farewell Party will take place in <strong>the</strong> middle of Luxembourg City. A tent will be erected on <strong>the</strong> famous Place Guillaume II in front<br />
of <strong>the</strong> town hall, food and drinks will be served. This is a perfect opportunity to also meet members of national brewing trade associations<br />
who have come to Luxembourg <strong>for</strong> <strong>the</strong> statutory meetings of The Brewers of Europe.<br />
17 34 TH EBC CONGRESS · LUXEMBOURG 26 – 30 MAY 2013
POSTER LIST / POSTER SESSION<br />
Poster Session on Tuesday, 28 May 2013 at 5.00 pm<br />
ABSTRACT TITLE FIRST AUTHOR INSTITUTE / COMPANY<br />
Session name / topic: Analysis (brewing raw materials, process, product, safety)<br />
P001 Soluble dietary fi ber as food ingredients from brewers‘ spent<br />
yeast polysaccharides<br />
P002 Si 3N 4-microsieves <strong>for</strong> a rapid detection of microbiological trace<br />
contaminations in beverages<br />
P003 A real-time next generation diagnostic tool <strong>for</strong> <strong>the</strong> malting and<br />
brewing industry: Selected ion fl ow tube mass spectrometry<br />
P004 Potential of near-infrared spectroscopy (NIR) in beer food chain:<br />
Applications in malting<br />
18<br />
Tiago Brandão<br />
Roland Novy<br />
Jessika De<br />
Clippeleer<br />
Paolo Fantozzi<br />
Unicer Bebidas, SA, Porto, Portugal<br />
Technische Universität München, Lehrstuhl für Brau-<br />
und Getränketechnologie, Freising, Germany<br />
KAHO St.-Lieven, Laboratory of Enzyme, Fermentation,<br />
and Brewing Technology (EFBT), Department Chemistry/<br />
Biochemistry - LFoRCe, Department M2S, KU Leuven,<br />
Gent, Belgium<br />
University of Perugia, Italian Brewing Research Centre<br />
(CERB), Perugia, Italy<br />
P005 Quantitative cleaning investigations using falling fi lm Enrico Fuchs Technische Universität Dresden, Faculty of Mechanical<br />
Engineering, Institute of Processing Machines and Mobile<br />
Machines, Dresden, Germany<br />
P006 Predicting <strong>the</strong> fl avor stability of beer using redox potential Dario Cotterchio Technische Universität München (TUM), Forschungszentrum<br />
Weihenstephan für Brau- und Lebensmittelqualität<br />
(BLQ), Freising, Germany<br />
P007 Classifi cation of malting barley according to harvest year and<br />
quality by using mid infrared spectroscopy and multivariate<br />
analysis<br />
P008 Microwave superheated water extraction of brewers´ spent<br />
grain arabinoxylans<br />
P009 Effect of brewing process on phenolic compounds and <strong>the</strong>ir<br />
corresponding antioxidant activities<br />
P010 Potential plant protecting substances from hops in dry-hopped<br />
beer<br />
Budour Ajib<br />
Tiago Brandão<br />
Celine Clayeux<br />
Annika Lagemann<br />
Lorraine University, Vandoeuvre les Nancy, France<br />
Unicer Bebidas, SA, Porto, Portugal<br />
TWISTAROMA, Colmar, France<br />
Bitburger Braugruppe GmbH, Bitburg, Germany<br />
P011 Protein-thiols in beer and throughout <strong>the</strong> brewing process Signe Hoff Copenhagen University, Food Science, Frederiksberg,<br />
Denmark<br />
P012 Analysis of fl avor active carbonyls in beer by derivatization with<br />
2,4-dinitrophenylhydrazine and HPLC-ESI-MS/MS<br />
P013 Evaluation of chemical physical stability of craft beers through<br />
unconventional methods<br />
P014 System and method <strong>for</strong> on-line monitoring of beer primary<br />
fermentation based on UV-VIS-SWNIR spectroscopy<br />
P015 Comparing optical versus traditional measurement technology<br />
in <strong>the</strong> brewery<br />
Nina Baumjohann<br />
Stefano Bertoli<br />
Pedro Rodrigues<br />
Daniel L Gore<br />
P016 Monitoring of <strong>the</strong> antioxidant potential of beer Edyta Kordialik-<br />
Bogacka<br />
VLB Berlin e.V., Central Laboratory, Berlin, Germany<br />
University of Udine, Department of Food Science, Udine,<br />
Italy<br />
Unicer Bebidas, SA, S. Mamede Infesta, Portugal<br />
Anton Paar GmbH, Process Instrumentation, Graz, Austria<br />
Lodz University of Technology, Institute of Fermentation<br />
Technology and Microbiology, Lodz, Poland<br />
P16a Radical scavenging ability of protein and peptide thiols in beer Marianne Lund University of Copenhagen, Department of Food Science,<br />
Frederiksberg C, Denmark<br />
Session name / topic: Beer quality: Foam & haze<br />
P017 The use of confocal laser scanning microscope (CLSM) <strong>for</strong> determination<br />
of fi ltration inhibiting substances in kieselguhr and<br />
membrane fi ltration<br />
Michael Kupetz<br />
TUM Weihenstephan, Lehrstuhl für Brau- und Getränketechnologie,<br />
Freising, Germany<br />
P018 Beta-glucan hazes - what's <strong>the</strong> problem? Cecilia Damiani Campden BRI, Nutfi eld, United Kingdom<br />
P019 Changes of metal concentration in beer production and <strong>the</strong>ir<br />
Infl uence on <strong>the</strong> mechanism <strong>for</strong> haze <strong>for</strong>mation<br />
Annika Burmeister<br />
TU Braunschweig, Institute of Food Chemistry, Braunschweig,<br />
Germany
ABSTRACT TITLE FIRST AUTHOR INSTITUTE / COMPANY<br />
P020 Improving your draught beer quality Johannes Tippmann Technische Universität München, Lehrstuhl für Brau-<br />
und Getränketechnologie, Freising, Germany<br />
P021 Slow and fast CO 2 escape from beer in relation to gushing Jan Savel Budejovicky Budvar, n.p., Research&Development,<br />
C.Budejovice, Czech Republic<br />
P022 Confounding expectations: Decoupling visual and fl avour properties<br />
of beer<br />
P023 Beverage Antioxidative IndeX (BAX) - a benefi cial index number<br />
<strong>for</strong> prognosis of <strong>the</strong> oxidative fl avour stability<br />
Paul Hughes<br />
Christian Müller<br />
Heriot-Watt University, School of Life Sciences,<br />
Edinburgh, United Kingdom<br />
Technische Universität Berlin, Department of Biotechnology,<br />
Lab of Brewing Science, Berlin, Germany<br />
P024 New aspects of beer glass infl uences on beer taste Petr Kosin Budeweiser Budvar, N.C., R&D, Ceske Budejovice, Czech<br />
Republic<br />
P025 Development and validation of an assay method <strong>for</strong> volatile<br />
fl avour compounds in GMP beer fl avour standards<br />
Boris Gadzov<br />
P027 A scientifi c look at <strong>the</strong> fl avour evolution of specialty beers Barbara Jaskula-<br />
Goiris<br />
P028 Volatile compounds screening of 14 commercial Alsatian beers<br />
by SBSE-LD-GC-MS<br />
P029 Addition of fermentable and non fermentable carbohydrates -<br />
impact on <strong>the</strong> yeast metabolism, sweetness, palate fullness and<br />
SO 2-content in beer<br />
P030 Investigation of <strong>the</strong> infl uence of a permease on diacetyl content<br />
during beer fermentation<br />
P031 A new insight <strong>for</strong> controlling <strong>the</strong> hop aroma using hydrophobicity<br />
of yeast cell surface<br />
Damien Steyer<br />
Torsten Seewald<br />
Nisha James<br />
Toru Kishimoto<br />
FlavorActiV Limited, Chinnor, United Kingdom<br />
KAHO St.-Lieven, Laboratory of Enzyme, Fermentation,<br />
and Brewing Technology (EFBT), Department Chemistry/<br />
Biochemistry - LFoRCe, Department M2S, KU Leuven,<br />
Gent, Belgium<br />
TWISTAROMA, Colmar, France<br />
Technische Universität Berlin, Lab of Brewing Science,<br />
Berlin, Germany<br />
Research and Teaching Institute <strong>for</strong> Brewing in Berlin<br />
(VLB), Department of Microbiology, Berlin, Germany<br />
ASAHI Breweries, Ltd., Research Laboratories <strong>for</strong> Brewing,<br />
Moriya-shi, Ibaraki, Japan<br />
P032 The perfect beer taster? Eleanor K Woods Campden BRI, Flavour and Innovation, Nutfi eld, United<br />
Kingdom<br />
P033 New hop technologies <strong>for</strong> special beer with unique fl avors Yoko Noro Kirin Company, Limited, Research & Development<br />
Division, Yokohama, Japan<br />
P034 Yeast biodiversity as a tool <strong>for</strong> fl avor improvement in beer Catrienus de Jong NIZO food research, Ede, The Ne<strong>the</strong>rlands<br />
P035 Characterisation of fresh and aged single-hop beers via hop oil<br />
constituents fi ngerprinting and sensory assessment<br />
P036 From wort to beer: The evolution of hoppy aroma of single hopped<br />
beers produced by early kettle hopping, late kettle hopping<br />
and dry hopping<br />
Filip Van Opstaele<br />
Michael Dresel<br />
KAHO Sint-Lieven University College, KU Leuven<br />
Association, Ghent, Belgium<br />
KAHO Sint-Lieven University College, KU Leuven Association,<br />
Laboratory of Enzyme, Fermentation and Brewing<br />
Technology, Ghent, Belgium<br />
P037 A simple concept to predict beer aging by use of statistical tools Annika Lagemann Bitburger Braugruppe GmbH, Bitburg, Germany<br />
P038 Different infl uences on generation of aging compounds and <strong>the</strong><br />
improvement of <strong>the</strong> oxidative beer fl avour stability using active<br />
packing material versus SO 2-additon<br />
P039 Re-inventing <strong>the</strong> wheel: sensory evaluation and <strong>the</strong> key to<br />
drinkability<br />
P39a Flavour and hop aroma stability of craft beers packed in different<br />
PET bottles<br />
Constanze Ruff<br />
Alex G Barlow<br />
Roland Folz<br />
Technische Universität Berlin, Lab of Brewing Science,<br />
Berlin, Germany<br />
ALL BEER, Sheffi eld, United Kingdom<br />
Versuchs- und Lehranstalt für Brauerei in Berlin (VLB)<br />
e.V., Berlin, Germany<br />
19 34 TH EBC CONGRESS · LUXEMBOURG 26 – 30 MAY 2013
ABSTRACT TITLE FIRST AUTHOR INSTITUTE / COMPANY<br />
Session name / topic: Brewing and malting microbiology<br />
P040 Microbiology of lambic sour beers processing unraveled with<br />
Matrix-Assisted Laser Desorption/Ionisation Time-of-Flight<br />
Mass Spectrometry (MALDI-TOF MS)<br />
P041 Exploration of matrix-assisted laser desorption/ionization-time<br />
of fl ight mass spectrometry (MALDI-TOF MS) as a fast identifi cation<br />
tool <strong>for</strong> beer spoilage bacteria<br />
P042 Molecular characterisation of lactic acid bacteria community<br />
during malting<br />
20<br />
Freek Spitaels<br />
Anneleen D. Wieme<br />
Sofi e Malfl iet<br />
Ghent University, Department of Biochemistry and<br />
Microbiology, Laboratory of Microbiology (LM-UGent),<br />
Ghent, Belgium<br />
University College Ghent, Faculty of Applied Bioscience<br />
Engineering, Laboratory of Biochemistry and Brewing,<br />
Ghent, Belgium<br />
KAHO St.-Lieven, Laboratory of Enzyme, Fermentation,<br />
and Brewing Technology (EFBT), Department Chemistry/Biochemistry<br />
- LFoRCe, Department M2S, KU<br />
Leuven, Gent, Belgium<br />
P043 Application of a new biological detoxifying method in malting Pedro Oliveira University College Cork, Cork, Ireland<br />
P044 Fast and reliable detection of beer spoilage bacteria <strong>for</strong> routine<br />
analysis- fi eld results<br />
P045 A novel tool to establish volatile molecular biomarkers to evaluate<br />
yeasts per<strong>for</strong>mance through beer fermentation: S. cerevisiae<br />
and S. pastorianus<br />
P046 Preventing <strong>the</strong> quality of high gravity beer from lactic contamination<br />
with lysozyme <strong>for</strong>mulation<br />
Session name / topic: Consumer preference testing<br />
P047 Consumer preference <strong>for</strong> sensory characteristics of commercial<br />
beers. Liking, disliking and appropriateness to different usage<br />
situations<br />
Session name / topic: Flavour chemistry<br />
Jürgen Günter Ziehl<br />
Cátia Martins<br />
Gilles Goemaere<br />
Gianluca Donadini<br />
Pall GmbH, Sales & Marketing, Bad Kreuznach,<br />
Germany<br />
University of Aveiro, Chemistry Department, QOPNA,<br />
Aveiro, Portugal<br />
SPINDAL AEB Group, Beverage Division, Gretz-Armainvilliers,<br />
France<br />
Università Cattolica del Sacro Cuore, Institute of<br />
Oenology and Food Engineering, Piacenza, Italy<br />
P048 A kinetic study of <strong>the</strong> <strong>for</strong>mation of 2- and 3-Methylbutanal Yarong Huang Technische Universität München, Lehrstuhl für Brau-<br />
und Getränketechnologie, Freising, Germany<br />
P049 Origin of <strong>the</strong> fl avour active aldehydes cis- and trans-4,5-Epoxy-2E-decenal<br />
Konrad Neumann<br />
VLB Berlin / TU Berlin, Berlin, Germany<br />
P050 Polyfunctional thiols in aged beer: Focus on <strong>the</strong> ribes off- fl avour Thi Thu Hang Tran Université catholique de Louvain, Unité de brasserie<br />
et des industries alimentaires, Earth and Life Institute<br />
ELIM, Louvain-la-Neuve, Belgium<br />
P051 Release of hop fl avours from cysteine conjugates by apotryptophanase<br />
P052 Odorant phenolic markers in chocolate malts. Fate of <strong>the</strong>m<br />
through beer ageing<br />
Session name / topic: Microbial physiology and genetics<br />
Sonia Collin<br />
Caroline Scholtes<br />
ELI - M (Applied Microbiology) / Université catholique<br />
de Louvain, Laboratoire de brasserie et des industries<br />
alimentaires (INBR), Louvain-la-Neuve, Belgium<br />
Université catholique de Louvain, ELIM - Laboratoire de<br />
brasserie et des industries alimentaires, Louvain-la-<br />
Neuve, Belgium<br />
P053 Sake fermentations: what can brewers learn Daniel Kerruish University of Nottingham, Nottingham, United Kingdom<br />
P054 Unraveling <strong>the</strong> yeast fl occulation mechanism at <strong>the</strong> molecular<br />
level<br />
Session name / topic: Barley breeding & agronomy<br />
P055 Worldwide breeding strategy <strong>for</strong> LOX-less barley and characteristics<br />
of new Australian malting barley variety 'Sou<strong>the</strong>rnStar'<br />
Francesco Ielasi<br />
Makoto Kihara<br />
Vrije Universiteit Brussel, Brussels, Belgium<br />
SAPPORO BREWERIES LTD., Bioresources Research<br />
and Development Department, Gunma, Japan
ABSTRACT TITLE FIRST AUTHOR INSTITUTE / COMPANY<br />
Session name / topic: Brewing and malting co-products<br />
P056 All-malt beer vs. non all-malt: how much corn does it take to<br />
make a difference? (industrial tests)<br />
Aleksander Poreda<br />
Session name / topic: Hops: Hop breeding, agronomy & processing<br />
University of Agriculture in Krakow, Department of<br />
Fermentation Technology and Technical Microbiology,<br />
Krakow, Poland<br />
P057 Hops: New opportunities <strong>for</strong> brewers Philippe Janssens Yakima Chief Inc., YC-Specialties Division, Louvain-la-<br />
Neuve, Belgium<br />
P058 Comparison of hop growing areas José Luis Olmedo Hijos de Rivera, S.A., R&D, La Coruña, Spain<br />
P059 Monitoring <strong>the</strong> appearance and composition of lupulin glands<br />
during maturation up to and beyond harvest<br />
Keith Westwood<br />
Barth Innovations Limited, Tonbridge, United Kingdom<br />
P060 Infl uence of picking date on <strong>the</strong> Hop Storage Index (HSI) Sandro Cocuzza Hopsteiner, Mainburg, Germany<br />
P061 Gushing induced by hop acid components - importance in various<br />
beers and beverages<br />
Session name / topic: Malting: Malt production & quality<br />
Brian Buffi n<br />
P062 Use of <strong>the</strong>rmal characterization during malting process Luciana Machado de<br />
Oliveira<br />
P063 Impact of barley variety and malting process on wort amino acid<br />
profi le and content<br />
P064 A possibility to decrease gluten in barley malt based beverages<br />
by using barley malt with optimized celiac-specifi c peptidase<br />
activity<br />
Marc Schmitt<br />
Roland Kerpes<br />
Kalsec, Inc., Kalamazoo, United States<br />
SENAI, Food Quality, Curitiba, Brazil<br />
IFBM, R&D, Vandoeuvre, France<br />
Lehrstuhl für Brau- und Getränketechnologie,<br />
Technische Universität München, Freising, Germany<br />
P065 Profi ling of metabolites´ and aroma developments during kilning Karin Gorzolka Bielefeld University, Proteome and Metabolome<br />
Research, Bielefeld, Germany<br />
P066 Predicting malt quality from barley gene expression Tommi Suvitaival Aalto University, Espoo, Finland<br />
P067 Accurate, quick and simple - Innovative level measurement of<br />
grain and malt<br />
Session name / topic: Novel raw materials <strong>for</strong> beer production<br />
P068 Impact of unmalted white and red sorghum [Sorghum bicolor<br />
(L.) Moench] on <strong>the</strong> quality and processability of mashes, worts<br />
and beers<br />
P069 Screening of fl avour compounds in beer and brewing materials<br />
using TRP channel assay<br />
Joachim Koelsch<br />
Birgit Schnitzenbaumer<br />
Chizuru Sato<br />
Siemens AG, Karlsruhe, Germany<br />
University College Cork, School of Food and Nutritional<br />
Sciences, Cork, Ireland<br />
SAPPORO BREWERIES LTD., Frontier Laboratories of<br />
Value Creation, Yaizu, Japan<br />
P070 Stabilization of beer with hop by-products Blanka Kotlíková Institute of Chemical Technology, Biotechnology,<br />
Prague, Czech Republic<br />
Session name / topic: End of beer processing, including auxiliary and process aids<br />
P072 Applicability of pectin as a stabilizing and fi ning agent <strong>for</strong> <strong>the</strong><br />
brewing process<br />
P073 Different aliphatic structures are <strong>the</strong> cause and <strong>the</strong> remedy<br />
against primary gushing<br />
Thomas Kunz<br />
Zahra<br />
Shokribousjein<br />
Technische Universität Berlin, Department of Biotechnology,<br />
Lab of Brewing Science, Berlin, Germany<br />
KULeuven, FBIW-M²S-MbS-LIBR/site Arenberg 33,<br />
Leuven (Heverlee), Belgium<br />
P074 Critical view on <strong>the</strong> calculation of pasteurization units in beer Knut Schwarzer University of Applied Sciences Ostwestfalen-Lippe,<br />
Institute of Live Science Technologies NRW, Lemgo,<br />
Germany<br />
P74a Validation possibilities of cleaning and disinfection (C&D) foam<br />
used <strong>for</strong> open point cleaning (OPC) of beverage fi lling lines<br />
Alexander Würtz<br />
VLB Berlin, BBSA, Berlin, Germany<br />
21 34 TH EBC CONGRESS · LUXEMBOURG 26 – 30 MAY 2013
ABSTRACT TITLE FIRST AUTHOR INSTITUTE / COMPANY<br />
Session name / topic: Microbial physiology and genetics<br />
P075 Three-dimensional structural analysis of kieselguhr particles<br />
and fi lter cakes<br />
P076 Impact of kieselguhr-free beer fi ltration on beer (fl avour) stability<br />
- a fi eld report<br />
Session name / topic: New process development<br />
22<br />
Katrin Mathmann<br />
Beatus Schehl<br />
Technische Universität München, Chair <strong>for</strong> Process<br />
Systems Engineering, Freising, Germany<br />
BASF South East Asia Pte Ltd, Nutrition & Health,<br />
Singapore, Singapore<br />
P077 Fine grinding and air classifi cation of barley malt Christian Nied Technische Universität München, Chair of Process<br />
Engineering of Disperse Systems, Freising, Germany<br />
P078 The "trickle-down effect" in fl ash pasteurization: A hydraulic<br />
problem and microbiological risk<br />
P079 Effi ciency of dry hopping procedure <strong>for</strong> improving <strong>the</strong> antioxidant<br />
activity of beer. Comparison with traditional hop boiling<br />
P080 Use of aroma condensates of brewery fermentation gas <strong>for</strong><br />
organoleptic optimization of alcohol-free beers<br />
Rudolf Michel<br />
Marie-Lucie<br />
Kankolongo Cibaka<br />
Thomas Tyrell<br />
P081 New method <strong>for</strong> de-alcoholization of beer Michael Dillenburger<br />
P082 MEURASTREAM: A global concept of wort production with reduced<br />
consumption of energy and process water<br />
P083 Making gluten-free beers with barley malt and a proline-specifi c<br />
endoprotease<br />
P084 Potential of aroma-modifi ed distiller's grains <strong>for</strong> <strong>the</strong> application<br />
in cereal based functional beverages<br />
Session name / topic: Wort production<br />
Ariane Dewulf<br />
Sylvie M Van<br />
Zandycke<br />
Magdalena Rückert<br />
GEA <strong>Brewery</strong> Systems GmbH, Technology, Kitzingen,<br />
Germany<br />
Université catholique de Louvain, Unité de brasserie<br />
et des industries alimentaires, Earth and Life Institute<br />
ELIM, Louvain-la-Neuve, Belgium<br />
VLB Berlin, Berlin, Germany<br />
Dillenburger & Hertel GmbH, Freising, Germany<br />
Meura sa, Research and Development, Louvain-la-<br />
Neuve, Belgium<br />
DSM Food Specialties, Enzyme Solutions, South Bend,<br />
United States<br />
Technische Universität München, Center of Life<br />
Sciences Weihenstephan, Chair of Brewing and<br />
Beverage Technology, Freising, Germany<br />
P085 Simulation of mashing process Simon Weber Technische Universität München, Chair <strong>for</strong> Process<br />
Systems Engineering, Freising, Germany<br />
P086 Investigation of a new prototype mashing system Tim J Hobley Technical University Denmark, Institute <strong>for</strong> Food,<br />
Lyngby, Denmark<br />
P087 Infl uence of <strong>the</strong> mashing regime on <strong>the</strong> phenolic character of<br />
wheat beer<br />
Session name / topic: Yeast and fermentation<br />
P088 Genedata Selector TM - enterprise genome management and<br />
analysis software to optimize fermentation processes<br />
P089 Process Analytical Technology - a paradigm change of systematic<br />
process evaluation and product release<br />
P090 Pilot VHG brewing: pure oxygen, yeast preconditions or higher<br />
temperatures?<br />
P091 Screening of brewing yeast strains <strong>for</strong> development of probiotic<br />
craft beers<br />
Frithjof Thiele<br />
Thomas Hartsch<br />
Stephan Birle<br />
José António<br />
Teixeira<br />
Monique Dillemans<br />
Radeberger Gruppe KG, Frankfurt, Germany<br />
Genedata AG, Basel, Switzerland<br />
Technische Universität München (TUM), Freising,<br />
Germany<br />
Universidade do Minho, Braga, Portugal<br />
Institut Meurice, Brewing Department, Brussels,<br />
Belgium<br />
P092 Reactivation of exhausted yeast physiological activity Mario Novak University of Zagreb, Faculty of Food Technology and<br />
Biotechnology, Department of Biochemical Engineering,<br />
Zagreb, Croatia<br />
P093 Evaluation of Near-Infrared Spectroscopy (NIRS) <strong>for</strong> on-line<br />
determination of critical process parameters <strong>for</strong> fermentation<br />
process control<br />
Lucas Vann<br />
North Carolina State University, Food, Bioprocessing &<br />
Nutrition Sciences, Raleigh, United States
ABSTRACT TITLE FIRST AUTHOR INSTITUTE / COMPANY<br />
P094 A systematic search <strong>for</strong> novel yeast strains with superior fermentation<br />
characteristics<br />
P095 Modeling <strong>the</strong> attenuation of extract during brewing operations:<br />
tracing <strong>the</strong> black box<br />
Jan Steensels<br />
R. Alex Speers<br />
KU Leuven, CMPG/VIB, Leuven, Belgium<br />
Dalhousie University, Process Engineering and Applied<br />
Science, Halifax, Canada<br />
P096 Optimisation of fermentation cooling profi le Elaine Martin Shepherd Neame, Faversham, United Kingdom<br />
P097 Comparison of growth kinetics, major metabolites and sensory<br />
profi les in brewing with non-saccharomyces yeast<br />
Session name / topic: Environmental issues and sustainability<br />
P098 Waste water handling in breweries, optimization and cost saving<br />
opportunities<br />
Panagiotis Tataridis<br />
Holger Schmidt<br />
Technological Educational Institute of A<strong>the</strong>ns,<br />
Department of Enology & Beverage Technology, A<strong>the</strong>ns,<br />
Greece<br />
Endress+Hauser Messtechnik GmbH+Co.KG, Industry<br />
Marketing, Weil am Rhein, Germany<br />
P099 <strong>Brewery</strong> carbon footprint: A case study Gordon Jackson Campden BRI, Environmental, Nutfi eld, United Kingdom<br />
P100 Improved strategies to reduce <strong>the</strong> caustic consumption in<br />
breweries<br />
P101 Holistic heat storage system <strong>for</strong> breweries - interface <strong>for</strong> renewable<br />
energy resources<br />
P102 From waste to renewable energy source - new technical approach<br />
in wet fermentation of brewers spent grains<br />
P104 Characterisation of co-products as a potential value added<br />
product <strong>for</strong> brewing and distilling<br />
P105 Utilization of brewer´s spent grain as medium <strong>for</strong> growing Rhodococcus<br />
erythropolis<br />
Joris de Grooth<br />
Tobias Becher<br />
Benjamin Haeffner<br />
Dawn L Maskell<br />
Enriqueta Martinez-<br />
Rojas<br />
Session name / topic: Management, training, maintenance and fi nancials<br />
Pentair, Research & Development, Enschede, The<br />
Ne<strong>the</strong>rlands<br />
Ziemann International GmbH, Process Technology, R+D,<br />
Ludwigsburg, Germany<br />
Technische Universität München, Center of Life<br />
Sciences Weihenstephan, Chair of Process Engineering<br />
of Disperse Systems, Freising, Germany<br />
Heriot-Watt University, School of Engineering and<br />
Physical Sciences, Edinburgh, United Kingdom<br />
VLB Berlin, Berlin, Germany<br />
P106 Vital role of brewery in-house R&D Adam Broz Budejovicky Budvar, n.p., Ceske Budejovice, Czech<br />
Republic<br />
Session name / topic: Beer, society, health and nutrition<br />
P107 The glycemic index - chance or threat <strong>for</strong> <strong>the</strong> beverage industry? Moritz Krahl Radeberger Gruppe KG, Frankfurt am Main, Germany<br />
P108 Transfer of Nitrate into Beer during Dry-hopping Stefan Hanke Bitburger Braugruppe GmbH, Versuchsbrauerei,<br />
Bitburg, Germany<br />
P109 Beer and cardiovsculare system - immediate respons after<br />
intake<br />
Ida Leskosek-<br />
Cukalovic<br />
Institute <strong>for</strong> Food Technology and Biochemistry, Faculty<br />
of Agriculture, University of Belgrade, Belgrade, Serbia<br />
23 34 TH EBC CONGRESS · LUXEMBOURG 26 – 30 MAY 2013
LECTURE ABSTRACTS<br />
L 01<br />
Brewing’s Got Talent – The Impact<br />
of Brewing on Science<br />
Ka<strong>the</strong>rine Smart 1<br />
1SABMiller plc, Woking, United Kingdom<br />
DESCRIPTION OF TOPIC:<br />
Brewing can be considered to be <strong>the</strong> most interesting<br />
combination of art, skill, technology,<br />
engineering and science. However, brewers<br />
are seldom given credit <strong>for</strong> <strong>the</strong>ir contributions<br />
to novel scientifi c discovery, despite an<br />
enviable heritage in this regard. During this<br />
presentation <strong>the</strong> impact of brewers on some<br />
of <strong>the</strong> most important scientifi c discoveries<br />
will be discussed. Many eminent scientists<br />
have been attracted to and intrigued by key<br />
brewing challenges and this presentation will<br />
explore what drives <strong>the</strong>se individuals towards<br />
our sector. Insights into <strong>the</strong> future of brewing<br />
science and engineering will be discussed.<br />
Ka<strong>the</strong>rine Smart<br />
Ka<strong>the</strong>rine Smart completed a BSc (Hons) in<br />
Biological Sciences at Nottingham University<br />
and was awarded <strong>the</strong> Rainbow Research<br />
Scholarship to complete a PhD in Brewing<br />
Yeast and Fermentation at Bass Brewers,<br />
Burton-on-Trent. This was w<strong>here</strong> her passion<br />
<strong>for</strong> brewing science began. She has held<br />
fellowships and academic positions at <strong>the</strong><br />
University of Cambridge, Ox<strong>for</strong>d Brookes University<br />
and most recently at <strong>the</strong> University<br />
of Nottingham w<strong>here</strong> she was <strong>the</strong> SABMiller<br />
Professor of Brewing Science. Ka<strong>the</strong>rine<br />
cites as her achievements <strong>the</strong> founding of <strong>the</strong><br />
National Brewing Library at Ox<strong>for</strong>d Brookes<br />
University and <strong>the</strong> Bioenergy and Brewing<br />
Science Centre at <strong>the</strong> University of Nottingham.<br />
During her academic career Ka<strong>the</strong>rine<br />
received several awards <strong>for</strong> her research<br />
including <strong>the</strong> Institute of Brewing and Distilling<br />
Cambridge Prize (1999), <strong>the</strong> prestigious<br />
Royal Society Industrial Fellowship (2001–<br />
2003), an Enterprise Fellowship (2002) and<br />
<strong>the</strong> Save British Science Award at <strong>the</strong> Houses<br />
of Parliament in <strong>the</strong> UK (2003). In 2012,<br />
she joined SABMiller as Group Chief Brewer.<br />
She also holds a Professorial role at <strong>the</strong> University<br />
of Nottingham. Ka<strong>the</strong>rine is a Fellow<br />
of <strong>the</strong> Institute of Brewing and Distilling, <strong>the</strong><br />
Royal Society of <strong>the</strong> Arts, Manufacturing and<br />
Commerce and <strong>the</strong> Society of Biology. She<br />
has served as Council and Board member <strong>for</strong><br />
<strong>the</strong> IBD, <strong>the</strong> ASBC and several Microbiology<br />
Societies. She has published extensively.<br />
L 02<br />
EBC and EBCU:<br />
Science and <strong>the</strong> beer consumer.<br />
A win-win relationship?<br />
Henri Reuchlin 1<br />
1Chairman of <strong>the</strong> <strong>European</strong> Beer Consumers Union (EBCU),<br />
St. Albans, United Kingdom<br />
DESCRIPTION OF TOPIC:<br />
EBC and EBCU might only diff er one confusing<br />
letter, but science and consumers are worlds<br />
apart. That is, from a consumers’ point of<br />
view. Most consumers will hardly ever think<br />
about science and beer, or science and food<br />
in general. If we do, it will be seldom in a positive<br />
way. Science is scary. It is opposed to<br />
all emotions surrounding our favourite beer<br />
brands: au<strong>the</strong>nticity, heritage and enjoyment.<br />
But is it really that sad? T<strong>here</strong> might be more<br />
in <strong>the</strong> relationship between science and <strong>the</strong><br />
beer consumer than meets <strong>the</strong> eye.<br />
The <strong>European</strong> Beer Consumers Union (EBCU)<br />
is a federation of thirteen independent national<br />
beer consumer organisations across<br />
Europe, with a combined membership of over<br />
170 000 members which represent <strong>the</strong> views<br />
and interests of beer consumers across Europe.<br />
Henri Reuchlin<br />
Henri Reuchlin has more than fi fteen years<br />
of experience in <strong>the</strong> brewing sector holding<br />
several positions within trade organisations.<br />
Henri has been involved in strategic cooperation<br />
projects in raw materials, logistics,<br />
environmental issues and public relations.<br />
In 2008 Henri founded BIERburo, providing<br />
management, commercial and communication<br />
services to <strong>the</strong> brewing industry in <strong>the</strong><br />
Ne<strong>the</strong>rlands. He is co-founder of <strong>the</strong> Foundation<br />
<strong>for</strong> Dutch Beer Education, Circle of Dutch<br />
Family Brewers and <strong>the</strong> Dutch Beer Week, as<br />
well as author of a book on beer and art. In<br />
November 2012 Henri Reuchlin was elected<br />
chairman of <strong>the</strong> <strong>European</strong> Beer Consumers<br />
Union. Henri holds a master in both biotechnology<br />
and corporate communication.<br />
24<br />
L 03<br />
The environmental per<strong>for</strong>mance of<br />
<strong>the</strong> <strong>European</strong> brewing sector<br />
John Koop 1<br />
1 KWA Bedrijfsadviseurs BV, Amersfoort, The Ne<strong>the</strong>rlands<br />
DESCRIPTION OF TOPIC:<br />
Brewers in Europe consider <strong>the</strong> environment<br />
as very important. They depend highly on <strong>the</strong><br />
environment <strong>for</strong> resources, both in quality<br />
and in quantity. A high level of environmental<br />
awareness is not only important to ensure<br />
that suffi cient resources of high quality are<br />
available, both now and in <strong>the</strong> future, but also<br />
to meet <strong>the</strong> general public‘s environmental<br />
expectations. T<strong>here</strong><strong>for</strong>e, The Brewers of Europe<br />
commissioned a study to describe <strong>the</strong><br />
environmental per<strong>for</strong>mance of <strong>the</strong> brewing<br />
sector in Europe.<br />
This paper presents <strong>the</strong> changes and trends<br />
in environmental per<strong>for</strong>mance over <strong>the</strong> years<br />
2008 to 2010 of 156 <strong>European</strong> breweries, representing<br />
62% of <strong>the</strong> total beer production<br />
volume in Europe. The key areas addressed<br />
are water, energy, greenhouse gases, secondary<br />
products, waste(water) and packaging.<br />
This quantitative data is supported and illustrated<br />
by a selection of prominent case studies.<br />
John Koop<br />
John Koop is a senior consultant watermanagement<br />
at KWA Bedrijfsadviseurs in The<br />
Ne<strong>the</strong>rlands. He assists companies in all water<br />
related issues, from water intake through<br />
use in processes to fi nally discharge or reuse.<br />
He is experienced in international water and<br />
energy saving projects in <strong>the</strong> beer and beverage<br />
industry.
L 04<br />
Worldwide energy and water effi ciency<br />
benchmark<br />
Taco Juriaanse 1, Gordon Jackson 2,<br />
Anastassia Johnson 3, Fons Pennartz 4<br />
1Dutch Brewers Association, Den Haag, The Ne<strong>the</strong>rlands,<br />
2Campden BRI, Brewing Services, Nutfi eld, United Kingdom,<br />
3Campden BRI, Environmental, Nutfi eld, United Kingdom,<br />
4KWA Business Consultants, Amersfoort, The Ne<strong>the</strong>rlands<br />
DESCRIPTION OF TOPIC:<br />
This presentation will include <strong>the</strong> results of<br />
<strong>the</strong> Worldwide Energy and Water Effi ciency<br />
Benchmark of <strong>the</strong> brewing industry based on<br />
<strong>the</strong> data <strong>for</strong> year 2011.<br />
This is <strong>the</strong> 4th energy benchmark and 2nd<br />
water benchmark undertaken by Campden<br />
BRI and KWA Business Consultants. We have<br />
carried out benchmarking projects on energy<br />
and water <strong>for</strong> <strong>the</strong> brewing sector worldwide<br />
in 1999, 2003, 2007 and 2011. More than 140<br />
breweries worldwide participated in <strong>the</strong> 2007<br />
energy and water benchmarking survey .<br />
The presentation will include <strong>the</strong> methodology<br />
of <strong>the</strong> study, main data points and overall<br />
trends of <strong>the</strong> brewing industry per<strong>for</strong>mance<br />
in energy and water use over <strong>the</strong> last 12 years<br />
and will highlight current best practice per<strong>for</strong>mance.<br />
Taco Juriaanse<br />
Sustainability and supply chain manager of<br />
<strong>the</strong> Dutch Brewers Association<br />
L 05<br />
Advances in hop aroma research -<br />
how much do we know today?<br />
Christina Schönberger 1<br />
1Barth Innovations, Nuremberg, Germany<br />
DESCRIPTION OF TOPIC:<br />
To unlock <strong>the</strong> mystery of hop aroma in beer is<br />
a research topic in brewing research <strong>for</strong> more<br />
than 100 years. However we are still not in <strong>the</strong><br />
position to explain what determines <strong>the</strong> intensity<br />
and <strong>the</strong> quality of a hop aroma in beer.<br />
This paper is intended to review <strong>the</strong> most important<br />
past activities in regard to hop aroma<br />
research and to present an comprehensive<br />
overview of <strong>the</strong> current knowledge. The paper<br />
outlines <strong>the</strong> challenges and shortcomings of<br />
<strong>the</strong> combination of very sophisticated analytic<br />
tools with sensory evaluations. A special focus<br />
is on <strong>the</strong> characterization of hop aroma<br />
and fl avours in highly hopped craft beers.<br />
Christina Schönberger<br />
Christina Schönberger obtained her diploma<br />
of brewing technologies in 1999 in Weihenstephan.<br />
After fi nishing her PhD <strong>the</strong>sis<br />
in 2003 she joined <strong>the</strong> German Brewers Association<br />
as a consultant. Since 2005 she is<br />
with Joh. Barth & Sohn as Technical Manager.<br />
Since 2010 she is part of <strong>the</strong> Barth Innovations<br />
Team as Technical Manager. Within her<br />
role she is responsible <strong>for</strong> <strong>the</strong> guidance of research<br />
projects and technical support. She is<br />
also head of <strong>the</strong> Barth Haas Hops Academy,<br />
established in 2011.<br />
L 06<br />
Craft brewing: The new global <strong>for</strong>ce<br />
and its implications <strong>for</strong> <strong>the</strong> brewing<br />
industry<br />
Larry Nelson 1<br />
1Advantage Publishing Ltd, Reigate, United Kingdom<br />
DESCRIPTION OF TOPIC:<br />
Microbrewing is now a global phenomenon.<br />
Craft brewers are increasingly a factor even<br />
in <strong>the</strong> most traditional of markets. How is<br />
<strong>the</strong>ir presence benefi cial to <strong>the</strong> brewing industry<br />
and what, if any, are <strong>the</strong> drawbacks<br />
presented by <strong>the</strong>ir emergence?<br />
What does this mean <strong>for</strong> <strong>the</strong> competitive<br />
structure of <strong>the</strong> industry, and how should established<br />
national and multinational brewers<br />
interact with <strong>the</strong>se thousands of newcomers?<br />
Could it be in <strong>the</strong> long term that multinational<br />
brewers are in dangerof becoming dinosaurs,<br />
or can <strong>the</strong>y adapt to <strong>the</strong>se new commercial<br />
realities?<br />
Larry Nelson<br />
Publisher and previously editor of Brewers‘<br />
Guardian, <strong>the</strong> website reporting on <strong>the</strong> brewing<br />
business, its people, and production and<br />
marketing innovations. Awarded MA Journalism,<br />
University of Westminster, London. A<br />
Canadian who has resided in England <strong>for</strong> <strong>the</strong><br />
past 20 years, living withing walking distance<br />
of several good pubs.<br />
25 34 TH EBC CONGRESS · LUXEMBOURG 26 – 30 MAY 2013
L 07<br />
Energy management isn’t about<br />
data, it’s about translating<br />
in<strong>for</strong>mation into action<br />
Nevil Wilson 1<br />
1Scanenergi Solutions AS, Kolding, Denmark<br />
DESCRIPTION OF TOPIC:<br />
Today utility costs can be <strong>the</strong> second biggest<br />
cost to <strong>the</strong> business. While <strong>the</strong> cost of obtaining<br />
data is low, creating value with it is far<br />
harder. Today’s market is fl ooded with software<br />
tools all claiming to address <strong>the</strong> pain<br />
points associated with data acquisition, validation<br />
and reporting – turning data into in<strong>for</strong>mation<br />
and <strong>the</strong>n action is w<strong>here</strong> <strong>the</strong> savings<br />
are made.<br />
Scanenergi Solutions AS (SeS) has worked on<br />
more than 50 breweries around <strong>the</strong> world in<br />
implementing utility management programmes<br />
addressing people and systems as much<br />
as technology. 30-50% of total savings on SeS<br />
utility management projects derive from behavioural<br />
change underpinned by data management,<br />
reporting and accountability. SeS<br />
will share <strong>the</strong>ir experiences and learning to<br />
assist o<strong>the</strong>rs in reducing <strong>the</strong> time to savings?<br />
What is new:<br />
Energy per<strong>for</strong>mance is a major infl uencer to<br />
<strong>the</strong> overall sustainability per<strong>for</strong>mance of a<br />
company. Knowing what you want from a utility<br />
management system from <strong>the</strong> outset will<br />
ensure you invest in <strong>the</strong> needs of today and<br />
tomorrow.<br />
Nevil Wilson<br />
Over 20 years’ experience in industrial energy<br />
effi ciency and energy management in a wide<br />
variety of sectors including brewing, food and<br />
drinks, tobacco, pulp and paper. Expert on<br />
energy management in<strong>for</strong>mation systems –<br />
joint author of guide on subject published by<br />
NRCan. Project responsibilities <strong>for</strong> people and<br />
systems changes needed to embed energy<br />
management into day to day business practices.<br />
Qualifi cations: BSc(Hons) Chem Eng,<br />
MBA, CEng, MEI, CPEnMS-Industrial<br />
L 08<br />
A novel water quality management<br />
strategy in breweries<br />
Verena Blomenhofer 1, Frauke Groß 1,<br />
Antonio Delgado 1, Tobias Becher 2<br />
1University of Erlangen-Nuremberg, Chair of Fluid Mechanics,<br />
Erlangen, Germany, 2Ziemann International GmbH,<br />
Ludwigsburg, Germany<br />
DESCRIPTION OF TOPIC:<br />
In this “Water Quality Management Project” a<br />
novel strategy of partial process water treatment<br />
is shown. Based on own investigations,<br />
water savings of 10-20% of <strong>the</strong> entire water<br />
consumption is possible with regard to displacement<br />
and rinsing water. Diff erent water<br />
qualities exist in breweries which can be separated<br />
in real-time, via <strong>the</strong> “Water Switch”,<br />
whose measurement techniques (IR-sensor,<br />
pH-value, temperature etc.) are presented.<br />
The water switch‘s recognition of <strong>the</strong> water<br />
quality is organized into three pollution levels.<br />
Each level has its own favorised treatment<br />
afterwards to gain an optimum in anaerobic<br />
wastewater treatment and reuse water quality.<br />
In combination with <strong>the</strong> water switch, <strong>the</strong><br />
generation of drinking water quality (reuse) is<br />
assured by an ultra-fi ltration membrane. The<br />
combination of <strong>the</strong> measurement signals is<br />
provided by a hybrid methodology that consists<br />
of a rule-based fuzzy-logic system and<br />
expert knowledge.<br />
Verena Blomenhofer<br />
In 2009 Verena Blomenhofer graduated as diploma<br />
engineer of Chemical- und Bioengineering<br />
at <strong>the</strong> University of Erlangen-Nuremberg.<br />
Since that time she works as a PhD student<br />
in a co-operation project between <strong>the</strong> Chair<br />
of Fluid Mechanics/University of Erlangen-<br />
Nuremberg and Ziemann International GmbH<br />
in <strong>the</strong> fi eld of research and development. Her<br />
main task is <strong>the</strong> development of a novel water<br />
quality management strategy in breweries by<br />
hybrid automation.<br />
26<br />
L 09<br />
Optimising <strong>the</strong> pre-treatment of<br />
Brewers‘ Grains to liberate sugars<br />
<strong>for</strong> bioethanol production<br />
David J Cook 1, Stuart Wilkinson 1,<br />
Francis Bealin-Kelly 2, Ka<strong>the</strong>rine A. Smart 2<br />
1University of Nottingham, Division of Food Sciences,<br />
Loughborough, United Kingdom, 2SABMiller plc, Woking,<br />
United Kingdom<br />
DESCRIPTION OF TOPIC:<br />
Brewers‘ Grains are a co-product of <strong>the</strong><br />
Brewing process <strong>for</strong> which value-added applications<br />
are sought. Being rich in cellulosic<br />
and hemicellulosic sugars, one option is to<br />
fractionate Brewer‘s grains into protein-rich<br />
(animal feed) and fi bre-rich fractions. The latter<br />
may be digested enzymically to liberate<br />
sugars as a substrate <strong>for</strong> bioethanol production.<br />
However, any pre-treatment processes<br />
required to overcome <strong>the</strong> structural integrity<br />
of Brewer‘s Grains must minimise energy or<br />
physico-chemical inputs in order to retain a<br />
net positive energy and cost balance on <strong>the</strong><br />
overall process.<br />
Here we present a comparison of pre-treatment<br />
strategies <strong>for</strong> Brewer‘s Grains (varying<br />
principally with regard to pH and temperature)<br />
in terms of <strong>the</strong> specifi c sugar yields<br />
attainable balanced against <strong>the</strong>ir required<br />
inputs. Treatment of Brewer‘s Grains with<br />
caustic or alkaline peroxide at ambient or<br />
moderate temperatures, were found to off er<br />
an eff ective, potentially cost-effi cient, pretreatment<br />
strategy.<br />
David J Cook<br />
David Cook is Lecturer in Brewing Science<br />
at <strong>the</strong> University of Nottingham, UK and is<br />
Course Director <strong>for</strong> <strong>the</strong>ir innovative e-learning<br />
based courses <strong>for</strong> Brewers. He is engaged<br />
in research across <strong>the</strong> malting and brewing<br />
fi elds, specialising in malting science, fl avour<br />
<strong>for</strong>mation, stability and perception. O<strong>the</strong>r current<br />
projects focus on biorefi ning and <strong>the</strong> use<br />
of lignocellulosic waste <strong>for</strong> bioethanol fermentations;<br />
links between crop husbandry,<br />
barley microbiology and <strong>the</strong> functionality of<br />
malts.
L 10<br />
Is it time to brew without boiling?<br />
Roland Feilner 1, Thomas Kunz 2,<br />
Frank-Jürgen Methner 2, Dirk Rehmann 3<br />
1Krones AG, Neutraubling, Germany, 2T.U. Berlin, Berlin,<br />
Germany, 3F.H. Weihenstephan, Weihenstephan, Germany<br />
DESCRIPTION OF TOPIC:<br />
Wort boiling has been continually optimised<br />
in terms of process engineering and energyeconomy.<br />
The latest research results, coupled with<br />
fundamental principles of <strong>the</strong>rmodynamics,<br />
now open up interesting approaches <strong>for</strong> very<br />
substantially reducing or completely eliminating<br />
this traditional boiling function, by using<br />
desorption, without requiring any additional<br />
energy. The presentation provides answers<br />
regarding <strong>the</strong> benefi ts, <strong>the</strong> advantages and<br />
<strong>the</strong> applications <strong>for</strong> a progressive reduction in<br />
total evaporation in <strong>the</strong> context of a brewery‘s<br />
overall energy concept. It presents <strong>the</strong> current<br />
limiting factors on <strong>the</strong> tasks involved in wort<br />
boiling, and <strong>the</strong> diff erent systems used. The<br />
focus <strong>here</strong> is on examining <strong>the</strong> evaporation<br />
behaviour of unwanted aromatics like DMSfree<br />
and <strong>the</strong> carbonyls so vital <strong>for</strong> <strong>the</strong> beer‘s<br />
stability. The eff ects of oxidative impairment<br />
on long-term stability are also addressed, in a<br />
comparison between boiled worts and beers,<br />
and those produced by means of desorption.<br />
Roland Feilner<br />
Roland Feilner (born in 1981), fi nished his<br />
apprenticeship as brewer and maltster. After<br />
that he studied food science technology<br />
in Weihenstephan and graduated in 2006 as<br />
an engineer. Currently he is responsible <strong>for</strong><br />
<strong>the</strong> new developments in wort treatment and<br />
process technology as a technical specialist<br />
in <strong>the</strong> Krones R&D division. Fur<strong>the</strong>rmore he‘s<br />
a Ph.D student at <strong>the</strong> T. U. of Berlin. His area of<br />
research is <strong>the</strong> reduction of unwanted fl avors<br />
in wort, with a desorption based stripping<br />
process.<br />
L 11<br />
Bio-based alternatives <strong>for</strong> polyester<br />
in packaging<br />
Ali Harlin 1, Thomas Gädda 1<br />
1VTT, Espoo, Finland<br />
DESCRIPTION OF TOPIC:<br />
Increasing concern of greenhouse gases and<br />
oil prizes are driving <strong>the</strong> industry even if fossil<br />
raw-material is available <strong>for</strong> plastics, which<br />
is a risk on PET markets. As well <strong>the</strong> environment<br />
issues and customer driven needs<br />
towards sustainability are increasing interest<br />
towards <strong>the</strong> bio based materials.<br />
General purpose plastics are hard to replace,<br />
because of have high-per<strong>for</strong>mance compared<br />
to price. PLA is used <strong>for</strong> beverage bottles.<br />
Barrier properties can be improved with polygycolic<br />
acid. VTT has proprietary technology<br />
in microbiological production of GA monomer<br />
and novel polymerization process of PGA by<br />
VTT.<br />
Drop-in bio-based PET has next step is to fi nd<br />
a bioreplacement <strong>for</strong> para tereftalic acid PTA,<br />
like <strong>the</strong> conversion of isobutanol into para-xylene.<br />
Furan dicarboxylic acid FDCA has been<br />
established biotechnical route enabling per<strong>for</strong>ment<br />
polyethylene furanadiacid PEF. Here<br />
VTT is developing own biotechnical routes to<br />
tereftalic acid FDCA.<br />
Ali Harlin<br />
Professor Harlin is professor <strong>for</strong> Bio-based<br />
materials in VTT, <strong>the</strong> Technical Research<br />
Centre of Finland and heading Bio-economy<br />
spearhead program in <strong>the</strong> fi eld of per<strong>for</strong>mance<br />
chemicals and industrial materials.<br />
He is also tutor of Finish Academy, Centre of<br />
Excellence – White Biochemistry and Green<br />
Chemistry in <strong>the</strong> fi eld of biomass-based monomers<br />
and polymers. He is aiming to create<br />
sustainable added value-chains and integrate<br />
<strong>the</strong>se into existing bio-refi neries.<br />
L 12<br />
Energy consumption and possibilities<br />
to reduce heat impact during<br />
hop kilning<br />
Ruslan Hofmann 1<br />
1Versuchs- und Lehranstalt für Brauerei in Berlin (VLB)<br />
e.V., Brewing & Beverage Science and Applications, Berlin,<br />
Germany<br />
DESCRIPTION OF TOPIC:<br />
After harvest green hops show water contents<br />
of 70 to 80% which must be reduced<br />
to approximately 10%. During <strong>the</strong> hop kilning<br />
process fresh air is heated to approximately<br />
65°C. A research project was started to decrease<br />
<strong>the</strong> consumption of fossil resources<br />
<strong>for</strong> heating and to recover volatile hop components<br />
from <strong>the</strong> exhaust air of <strong>the</strong> hop kiln. In a<br />
second stage <strong>the</strong> air fl ow should be optimized<br />
to fur<strong>the</strong>r improve <strong>the</strong> drying process.<br />
The collected materials were analysed using<br />
extraction and subsequent GC/MS. For quantifi<br />
cation of <strong>the</strong> results an isotope dilution assay<br />
was used. In <strong>the</strong> exhaust air <strong>the</strong> quantifi ed<br />
hop oils summed up to at least 0.25 mg hop<br />
oils per h and m3. In <strong>the</strong> mean, fresh air was<br />
heated up 9K. The heat recovery resulted in<br />
an effi ciency > 50%. In <strong>the</strong> new designed pilot<br />
dryer <strong>the</strong> complete air fl ow was used <strong>for</strong><br />
heat recovery.The new design should lead to<br />
fur<strong>the</strong>r energy savings. The use of several<br />
temperature and humidity probes was used<br />
to monitor <strong>the</strong> drying process.<br />
Ruslan Hofmann<br />
Ruslan Hofmann started his brewing career<br />
at <strong>the</strong> Berliner Bürgerbäu Brauerei. In 2008<br />
he received his diploma degree in brewing<br />
technology at <strong>the</strong> Technische Universität Berlin<br />
(TUB). In <strong>the</strong> same year he started working<br />
<strong>for</strong> <strong>the</strong> VLB Berlin e.V. w<strong>here</strong> he specialized<br />
in packaging fi rst. In 2010 he moved to <strong>the</strong><br />
Department Brewing & Beverage Science and<br />
Applications. Main scope of his work are research<br />
projects on fl avour stability and hop<br />
quality from harvest to beer.<br />
27 34 TH EBC CONGRESS · LUXEMBOURG 26 – 30 MAY 2013
L 13<br />
Principal components analysis of<br />
aroma compounds in beer<br />
Taku Irie 1,Yuichi Nakamura 1, Yoshinori Ito 1<br />
1Asahi Breweries Ltd., Tokyo, Japan<br />
DESCRIPTION OF TOPIC:<br />
In this study, we examined <strong>the</strong> possibility of<br />
principal component analysis (PCA) to control<br />
quality of beer fl avour, through <strong>the</strong> interpretation<br />
of <strong>the</strong> results about a number of aroma<br />
compounds which are syn<strong>the</strong>sized by yeast<br />
metabolism.<br />
We made PCA of 6 compounds (acetate esters<br />
and higher alcohols) of a certain kind of our<br />
product beer (n = 322). Then we assumed <strong>the</strong><br />
interpretation, by analyzing <strong>the</strong> correlation<br />
between <strong>the</strong> PC scores and o<strong>the</strong>r parameters<br />
and by test brews under various amounts and<br />
timings of wort aeration which aff ect <strong>the</strong> ester<br />
<strong>for</strong>mation.<br />
As a result <strong>the</strong> data are abridged into two<br />
components, which are assumed to indicate<br />
each of two steps of ester <strong>for</strong>mation during<br />
fermentation. And <strong>the</strong> results of test brews<br />
suggest that this method could be possible to<br />
describe and evaluate <strong>the</strong> confl icting eff ects<br />
of oxygen on <strong>the</strong> each step, that is, acceleration<br />
by <strong>the</strong> increase of <strong>the</strong> metabolic substances<br />
and deceleration by <strong>the</strong> inhibition to<br />
alcohol acetyltransferase.<br />
Taku Irie<br />
He received a M.S. degree in engineering from<br />
<strong>the</strong> University of Tokyo in 2000 and began<br />
working <strong>for</strong> Asahi Breweries, Ltd.. After he<br />
had worked in <strong>the</strong> packaging section at some<br />
breweries to 2007, he started his career as<br />
an engineer of technological development of<br />
brewing at <strong>the</strong> Ibaraki R&D Promotion Offi ce.<br />
And since May in 2012, he has been working<br />
as a guest researcher at <strong>the</strong> Chair of Brewing<br />
and Beverage Technology, Center of Life and<br />
Food Sciences Weihenstephan.<br />
L 14<br />
A new stable isotope dilution approach<br />
<strong>for</strong> <strong>the</strong> sensitive quantitation<br />
of 3-methyl-2-buten-1-thiol (MBT)<br />
and its application to study sunstruck-fl<br />
avour development in beer<br />
and its application to study sunstruck-fl<br />
avour development in beer<br />
Susanne Stingl 1, Peter Schieberle 1,2<br />
1German Research Centre <strong>for</strong> Food Chemistry, Freising, Germany,<br />
2Technical University of Munich, Chair Food Chemistry,<br />
Freising, Germany<br />
DESCRIPTION OF TOPIC:<br />
Beer, when exposed to sunlight, quickly develops<br />
an unpleasant aroma, commonly referred<br />
to as <strong>the</strong> “sunstruck-fl avour”. This off -<br />
fl avour is predominantly caused by skunky<br />
smelling 3-methyl-2-buten-1-thiol (MBT), <strong>for</strong>med<br />
in <strong>the</strong> course of a light induced reaction<br />
involving isohumulone, ribofl avin, and cysteine.<br />
Due to its extremely low odour threshold,<br />
even minimum amounts of MBT may have a<br />
detrimental eff ect on <strong>the</strong> aroma of beer.<br />
In order to get a deeper insight into <strong>the</strong> parameters<br />
infl uencing MBT <strong>for</strong>mation in beer<br />
and o<strong>the</strong>r hop based beverages, we developed<br />
a new method <strong>for</strong> its sensitive and reliable<br />
quantitation. Using 4-vinylpyridine, MBT<br />
was converted to its pyridinylethyl derivative,<br />
which was fi nally analysed by LC-MS/MS. Following<br />
<strong>the</strong> stable isotope dilution approach,<br />
deuterium labeled MBT was used as <strong>the</strong> internal<br />
standard. The method was <strong>the</strong>n applied<br />
to study <strong>the</strong> MBT <strong>for</strong>mation in model systems,<br />
depending on hop product, sulphur source,<br />
and <strong>the</strong> presence of antioxidants.<br />
Susanne Stingl<br />
Born in 1984. Studied Food Chemistry at <strong>the</strong><br />
Technische Universität München (Technical<br />
University of Munich) in Garching, Germany.<br />
She graduated in 2008. Currently, she works<br />
as a Research Scientist at <strong>the</strong> Deutsche Forschungsanstalt<br />
für Lebensmittelchemie (German<br />
Research Centre <strong>for</strong> Food Chemistry),<br />
Freising-Weihenstephan, in <strong>the</strong> group of Prof.<br />
Dr. Peter Schieberle. At <strong>the</strong> moment she is<br />
about to fi nish her PhD studies on compounds<br />
contributing to <strong>the</strong> sunstruck off -fl avour in<br />
beer.<br />
28<br />
L 15<br />
A kinetic framework <strong>for</strong> <strong>the</strong> modelling<br />
of beer fl avour instability<br />
Paul Hughes 1<br />
1International Centre <strong>for</strong> Brewing and Distilling, Edinburgh,<br />
United Kingdom<br />
DESCRIPTION OF TOPIC:<br />
Many problems of fl avour quality have not effectively<br />
yielded to a solution, despite many<br />
man years of eff ort. In part this is due to <strong>the</strong><br />
compositional complexity of beer, so that research<br />
has necessarily focused on one or a<br />
small number of issues. This is particularly<br />
pertinent <strong>for</strong> beer fl avour instability, w<strong>here</strong><br />
eff ort variously focuses on oxygen, sulphite,<br />
lipid oxidation, aldehydes, Strecker degradation<br />
etc. Here we propose a framework <strong>for</strong><br />
addressing <strong>the</strong> connectivity of <strong>the</strong>se various<br />
elements at <strong>the</strong> chemical level, and identify<br />
knowledge gaps that, when fi lled will enable<br />
<strong>the</strong> prediction of <strong>the</strong> trajectories of beer fl avour<br />
deterioration with time. As an example<br />
we will look in detail at <strong>the</strong> various routes of<br />
sulphite loss during beer ageing and indicate<br />
what would be required to minimise <strong>the</strong> rate<br />
of sulphite loss.<br />
Paul Hughes<br />
Paul is a trained chemist and is currently Professor<br />
of Brewing and Distilling at Heriot-Watt<br />
University. He specialises in fi nal beer quality,<br />
sensory research/methodology, whisky maturation,<br />
hop chemistry and business planning<br />
<strong>for</strong> innovation. Paul consults regularly<br />
<strong>for</strong> <strong>the</strong> food and drinks industries and has<br />
published widely.
L 16<br />
Amino acid oxidation - an unrevealed<br />
pathway leading to <strong>the</strong><br />
<strong>for</strong>mation of staling aldehydes in<br />
bottled beer?<br />
Philip C Wietstock 1, Thomas Kunz 1,<br />
Constanze Ruff 1, Frank-Jürgen Methner 1<br />
1Technische Universität Berlin, Lab of Brewing Science,<br />
Berlin, Germany<br />
DESCRIPTION OF TOPIC:<br />
Off -fl avor in lager beer is often associated<br />
with <strong>the</strong> appearance of staling aldehydes (SA).<br />
Previous storage experiments suggest that<br />
Fe 2+ and O 2 promote <strong>the</strong> rate of beer staling.<br />
Known mechanisms, such as Strecker degradation<br />
of amino acids (AA), give no explanation<br />
<strong>for</strong> this phenomenon. Additional storage<br />
trials were conducted to fur<strong>the</strong>r assess this<br />
observation. A commercially available lager<br />
beer was dosed w/ AAs and Fe 2+, and stored<br />
<strong>for</strong> 6 weeks (dark, 28 °C, w/ and w/o O 2 in<br />
headspace). Bottles w/o additions served as<br />
references. AA and SA levels were measured<br />
during storage and <strong>the</strong> oxidative beer stability<br />
was monitored using electron spin resonance<br />
(ESR) spectroscopy. The experiment confi rmed<br />
that SA levels signifi cantly correlate with<br />
both, AA and Fe levels, and are increased by<br />
presence of O 2.<br />
Trials using model solutions revealed that<br />
direct oxidation of leucine via Fe-H 2O 2 yields<br />
3-methylbutanal. An unrevealed staling mechanism<br />
via oxidation of AAs in bottled beer<br />
is proposed.<br />
Philip C Wietstock<br />
Philip Wietstock is a scientifi c assistant at <strong>the</strong><br />
Technische Universität Berlin, Germany. After<br />
graduating his biotechnology studies with<br />
qualifi cation as a Dipl.-Ing. at <strong>the</strong> Technische<br />
Universität Berlin (2009), he was working <strong>for</strong><br />
one year at <strong>the</strong> Department of Food Science<br />
and Technology at <strong>the</strong> Oregon State University,<br />
Corvallis, USA. In 2011, he transferred to<br />
his present profession w<strong>here</strong> he is working<br />
on his degree dissertation about brewing parameters<br />
infl uencing oxidative beer stability.<br />
L 17<br />
Detoxifi cation of off -fl avor unsaturated<br />
carbonyl compounds:<br />
Purifi cation and characterization of<br />
enone reductases from yeast with<br />
new catalytic activities<br />
Leif A Garbe 1, Enriqueta Martinez-Rojas 1<br />
1TUB / VLB, Bioanalytics, Berlin, Germany<br />
DESCRIPTION OF TOPIC:<br />
Two NAD(P)H dependent enone reductases<br />
(ER) from brewing yeast were purifi ed and<br />
characterized. They reduce e.g. 2E-nonenal<br />
into nonanal which is exhibiting a higher fl avor<br />
threshold. Extreme fl avor active 4,5-epoxy-2E-decenal<br />
(metallic fl avor) is reduced<br />
into 4,5-epoxydecanal by ER. In addition, we<br />
found a second brewing relevant mode of action.<br />
ER is able to trans<strong>for</strong>m α/β-unsaturated<br />
alcohols into saturated ketones. The peroxidation<br />
/ reduction product of linoleic acid is<br />
13S-hydroxy-9Z,11E-octadecadienoic acid<br />
(13-HODE) which has been reported as off -<br />
fl avor and tri-hydroxy fatty acid precursor.<br />
The latter reduces foam stability. When 13-<br />
HODE is incubated with pure ER, 13-oxo-9Zoctadecenoic<br />
acid was analyzed as product.<br />
Saturated ketones can be reduced into alcohols<br />
or directly metabolized. In consequence,<br />
ER from yeast can detoxify off fl avor relevant<br />
unsaturated carbonyls and also act as key enzymes<br />
in <strong>the</strong> metabolism of linoleic acid hydroxides<br />
resulting from lipid oxidation.<br />
Leif A Garbe<br />
Leif-Alexander Garbe is Professor <strong>for</strong> Biochemical-<br />
and Technical Analysis at <strong>the</strong> TU Berlin<br />
and also chairs <strong>the</strong> Special Analyses at VLB<br />
Berlin. Leif graduated with a diploma in chemistry.<br />
He fi nished his Ph.D. in 2002 on <strong>the</strong><br />
Metabolism of Hydroxy-fatty Acids in Yeasts<br />
and his habilitation in 2009 on The Biochemistry<br />
of Oxidized Lipids: Analysis of Bioactive<br />
Metabolites. His research interest focuses<br />
on biotrans<strong>for</strong>mation and trace analysis with<br />
isotope dilution techniques and MS.<br />
L 18<br />
Quantitation of desirable aroma-active<br />
and undesirable toxicologically<br />
relevant vinyl aromatic compounds<br />
in wheat beer<br />
Daniel Langos 1, Michael Granvogl 2,<br />
Peter Schieberle 1<br />
1Deutsche Forschungsanstalt für Lebensmittelchemie,<br />
Freising, Germany, 2Technische Universität München,<br />
Freising, Germany<br />
DESCRIPTION OF TOPIC:<br />
2-Methoxy-4-vinylphenol and 4-vinylphenol<br />
are important odorants in wheat beer, <strong>for</strong>med<br />
from ferulic and p-coumaric acid. But, by this<br />
way, also <strong>the</strong> toxicologically relevant styrene<br />
is generated from cinnamic acid. T<strong>here</strong><strong>for</strong>e,<br />
breweries have <strong>the</strong> challenging task to produce<br />
beers with reduced styrene content but<br />
without changing <strong>the</strong> aroma. Thus, <strong>the</strong> aims<br />
of <strong>the</strong> study were<br />
(i) to analyse <strong>the</strong> most important odorants of<br />
wheat beer and<br />
(ii) to correlate <strong>the</strong> amounts of vinyl aromatics<br />
and <strong>the</strong>ir corresponding phenolic acids<br />
(precursor) in all process steps of beer production.<br />
Quantitation via stable isotope dilution analysis<br />
and calculation of odour activity values<br />
(OAV) revealed 2-methoxy-4-vinylphenol (OAV<br />
= 20) and 4-vinylphenol (11) as important aroma<br />
compounds. Analysis of process intermediates<br />
showed that decarboxylation of phenolic<br />
acids mainly occurs during fermentation<br />
and <strong>for</strong>mation of 2-methoxy-4-vinylphenol<br />
and 4-vinylphenol is always accompanied by<br />
styrene <strong>for</strong>mation.<br />
Daniel Langos<br />
2004-2009: Study of food chemistry at <strong>the</strong><br />
Technical University of Munich since 2009:<br />
PhD student in <strong>the</strong> working group of Prof. Dr.<br />
Peter Schieberle<br />
29 34 TH EBC CONGRESS · LUXEMBOURG 26 – 30 MAY 2013
L 19<br />
Infl uence of selected hop and beer<br />
substances on gushing<br />
Jean Titze 1, Antonie Herrmann 2, Zahra<br />
Shokribousjein 3, Sylvie Deckers 3, Christina<br />
Schönberger 4, Guy Derdelinckx 3, Vladimír<br />
Ilberg 5<br />
1National University of Ireland, University College Cork, Cork,<br />
Ireland, 2University of Applied Science Weihenstephan-<br />
Triesdorf, Institute of Food Technology, Freising, Germany,<br />
3KU Leuven, Department of Microbial and Molecular Systems,<br />
Leuven, Belgium, 4Barth-Haas Group, Barth Innovations, Joh.<br />
Barth und Sohn, Nuremberg, Germany, 5University of Applied<br />
Science Weihenstephan-Triesdorf, Faculty of Gardening and<br />
Food Technology, Freising, Germany<br />
DESCRIPTION OF TOPIC:<br />
As gushing is still a problem <strong>for</strong> <strong>the</strong> brewing<br />
industry, it is worldwide in <strong>the</strong> technological<br />
and scientifi c fi eld of interest. In this context<br />
<strong>the</strong> question was raised: Which specifi c<br />
substances in beer and especially in hop are<br />
responsible <strong>for</strong> gushing reduction or suppression?<br />
Phenolic and fatty acids as well as constituent<br />
parts of <strong>the</strong> essential hop oils were<br />
examined. It turned out, that some substances<br />
exist which can cause gushing itself. Two<br />
reasons are possible:<br />
(1) Due to interaction between phenolic acids<br />
via hydrogen bonds, larger molecules are <strong>for</strong>med<br />
w<strong>here</strong> CO 2 can be released.<br />
(2) According to <strong>the</strong> multi-functional groups<br />
CO 2 can directly interact via hydrogen bonds<br />
with phenolic acids.<br />
The gushing positive eff ect of phenolic and<br />
fatty acids could be compensated by <strong>the</strong> addition<br />
of constituent parts of <strong>the</strong> essential hop<br />
oils, w<strong>here</strong> some monoterpenes are characterized<br />
by suppressing and/or reducing gushing.<br />
Besides, o<strong>the</strong>r monoterpenes have no<br />
relevant eff ect on <strong>the</strong> reduction.<br />
Jean Titze<br />
Dr. Jean Titze studied Technology and Biotechnology<br />
of Food at <strong>the</strong> Technical University<br />
of Munich as well as <strong>European</strong> and national<br />
Food & Feed Law at <strong>the</strong> Academy of Food<br />
Law, Philipps-University of Marburg. Having<br />
worked several years as a <strong>Brewery</strong> Consultant,<br />
he joined Deloitte & Touche as a Senior<br />
Consultant, focusing on <strong>the</strong> food and beverage<br />
industry. In <strong>the</strong> last two years he lived<br />
in Ireland working as a Senior Research Scientist<br />
and brewery manager <strong>for</strong> <strong>the</strong> National<br />
University of Ireland at <strong>the</strong> University College<br />
Cork. He is also a lecturer <strong>for</strong> food law at <strong>the</strong><br />
University of Applied Science Weihenstephan-<br />
Triesdorf.<br />
L20<br />
Hop lipids - A comprehensive<br />
overview with regard to beer foam,<br />
fl avour (in)stability and gushing<br />
Nils Rettberg 1, Leif-Alexander Garbe 1<br />
1VLB Berlin / TU Berlin, Research Institute <strong>for</strong> Special<br />
Analysis / Chair of Bioanalytics, Berlin, Germany<br />
DESCRIPTION OF TOPIC:<br />
For decades lipids and <strong>the</strong>ir oxidation products<br />
have attracted brewer‘s attention. They<br />
strongly infl uence beer foam and fermentation,<br />
are closely linked to beer fl avour (in)stability<br />
and may promote gushing. In <strong>the</strong> past,<br />
lipid analysis in brewing focused on adjuncts,<br />
malt, wort, yeast, and beer. Lipid analysis<br />
from hops is particularly challenging, thus in<strong>for</strong>mation<br />
and data on hop lipids is rare. Also<br />
<strong>the</strong>ir role in brewing, especially in terms of<br />
currently popular dry and late hopping techniques,<br />
is unclear.<br />
The current paper gives a fi rst comprehensive<br />
overview on <strong>the</strong> total lipid content and<br />
lipid composition of several hop varieties and<br />
products. The distribution and concentration<br />
of short and long chained fatty acids, as well<br />
as <strong>the</strong> occurrence of prominent oxidation products<br />
is described. In addition to analytical<br />
techniques and experimental data, <strong>the</strong> relevance<br />
of hop lipids <strong>for</strong> <strong>the</strong> fi nal product is<br />
marked.<br />
Nils Rettberg<br />
Nils is a trained brewer holding a diploma<br />
in biotechnology from <strong>the</strong> Berlin Institute of<br />
Technology (TUB). Since 2011 he is a Ph.D.<br />
student at <strong>the</strong> TUB chair of bioanalytics and<br />
employed at <strong>the</strong> “department <strong>for</strong> special<br />
analyses” at VLB Berlin. Nils’ work includes<br />
several student courses ranging from basic<br />
chemical-technical analysis to sophisticated<br />
(bio) analytical techniques. His research focuses<br />
on brewing relevant special analyses<br />
using mass spectrometry and stable isotope<br />
dilution technique.<br />
30<br />
L 21<br />
Tool <strong>for</strong> predicting <strong>the</strong> risk of early<br />
gushing from harvest to come<br />
Patrick Boivin 2, Régis Fournier 1<br />
1IFBM, Biology Molecular, Vandoeuvre, France, 2IFBM, Vandoeuvre,<br />
France<br />
DESCRIPTION OF TOPIC:<br />
The gushing phenomenon is a problem <strong>for</strong> <strong>the</strong><br />
brewing and occurred more often in <strong>the</strong> last<br />
years than be<strong>for</strong>e. The occurrence of gushing<br />
is mainly attributed to <strong>the</strong> malt due to fungal<br />
infection of barley. The biggest challenge of<br />
malting industry is to produce malt with no<br />
tendency to gush. To produce a n gushing negative<br />
malt, maltsters had to know <strong>the</strong> gushing<br />
potential of barley lot and even to know<br />
<strong>the</strong> risk from harvest to come from diff erent<br />
malting barley production zones. In this project,<br />
we identifi ed <strong>the</strong> gushing potential of<br />
each Fusarium species that infected malting<br />
barley in <strong>the</strong> fi eld, developed a QPCR tool to<br />
quantify <strong>the</strong>m in during <strong>the</strong> vegetation period<br />
and in <strong>the</strong> fi nal grain. With this QPCR tool, data<br />
obtained from diff erent years, we are able to<br />
predict after <strong>the</strong> fl owering period, <strong>the</strong> risk of<br />
gushing of malting barley cultivated in diff erent<br />
sites.<br />
Patrick Boivin<br />
He is Scientifi c Director at IFBM. He received<br />
PhD in Microbiology, Enzymology and Bioconversion<br />
from Compiègne University (1987). He<br />
was Post-Doctoral Fellow at Baylor University,<br />
Texas, U.S.A., 1987-1989. He received Master<br />
in Business and Administration in 1998 from<br />
French Institute of Management. Since 1989<br />
he has been working at IFBM. He is a member<br />
of <strong>the</strong> EBC Brewing Science Group and<br />
a member of <strong>the</strong> French Barley-Malt-Beer<br />
Committee. He has published several papers,<br />
reviews and patents
L 22<br />
Malting at low moisture to save<br />
energy<br />
Marc Schmitt 1, Patrick Boivin 1<br />
1IFBM, Vandoeuvre, France<br />
DESCRIPTION OF TOPIC:<br />
Malt is <strong>the</strong> main raw material and strategic<br />
<strong>for</strong> <strong>the</strong> brewer to produce a nice beer without<br />
process problem. To get good malt with new<br />
environmental constraints which mean using<br />
less energy and water is <strong>the</strong> biggest challenge<br />
of malting industry.<br />
To be usable by brewers, barley has to be<br />
trans<strong>for</strong>med in malt. Kilning is a drying step<br />
that uses a large amount of energy (500 to<br />
1000 KWh/ton and 90% of energy used in<br />
malting process). The main objective of <strong>the</strong><br />
project was to produce high quality malt with<br />
barley containing less water during germination.<br />
To achieve this objective, a better understanding<br />
of key physico-chemical and biochemical<br />
phases were necessary. Validation of<br />
research at semi-industrial scale showed that<br />
malting at low moisture was possible with a<br />
production of good beer.<br />
Marc Schmitt<br />
Marc SCHMITT is raw material and biochemistry<br />
Manager at IFBM. He joined Qualtech at<br />
<strong>the</strong> beginning of 2002 as manager of <strong>the</strong> Malting<br />
& Brewing Laboratory. He has been lecturer<br />
in biochemistry and biosciences engineering at<br />
Nancy University from which he received his<br />
PhD in food biochemistry which was awarded<br />
by Nancy University (1996). He is a member of<br />
several working groups of French Association of<br />
standardization (AFNOR). Member of <strong>the</strong> Analysis<br />
Committee of <strong>the</strong> EBC since May 2004.<br />
L 23<br />
The infl uence of <strong>the</strong> kilning process<br />
on <strong>the</strong> gelatinization temperature of<br />
barley malt<br />
Michael Rittenauer 1, Martina Gastl 2, Thomas<br />
Becker 2<br />
1TUM Weihenstephan, AG Raw Materials, Freising, Germany,<br />
2Technische Universität München, AG Raw Materials, Freising,<br />
Germany<br />
DESCRIPTION OF TOPIC:<br />
The appearance of high gelatinization temperatures<br />
(GT) in malt is an unknown, periodic<br />
eff ect which causes problems during <strong>the</strong> entire<br />
brewing process. The GT must be realized<br />
during mashing <strong>for</strong> <strong>the</strong> enzymatic conversion.<br />
High mashing temperatures however, harm<br />
<strong>the</strong> amylolytic enzymes. This double bind<br />
leads to insuffi cient degradation of starch,<br />
lower fi nal attenuation and fur<strong>the</strong>r related<br />
problems. The GT of raw materials itself cannot<br />
be infl uenced by brewers or maltsters.<br />
Results from diff erent authors describe an<br />
increase during <strong>the</strong> malting process but <strong>the</strong><br />
reason t<strong>here</strong><strong>for</strong>e is unclear. An increase of GT<br />
due to a <strong>the</strong>rmal treatment below <strong>the</strong> GT is<br />
called annealing.<br />
To verify whe<strong>the</strong>r this eff ect occurs during <strong>the</strong><br />
killning process, samples of green malt were<br />
dryed at standard- and annealing en<strong>for</strong>cing<br />
conditions. The GT was determined be<strong>for</strong>e<br />
and after <strong>the</strong> <strong>the</strong>rmal treatment. The results<br />
show <strong>the</strong> contiguity of <strong>the</strong> process parameters<br />
during killinig and <strong>the</strong>ir infl uence to <strong>the</strong><br />
GT.<br />
Michael Rittenauer<br />
In July 2010 Michael Rittenauer received his<br />
Diploma Engineering Degree from <strong>the</strong> Technical<br />
University of Munich in Brewing Science<br />
and Beverage Technology. Since he is working<br />
as a scientist at <strong>the</strong> chair of Brewing- and<br />
Beverage Technology in Weihenstephan. The<br />
topic of his PhD Thesis is <strong>the</strong> optimization of<br />
<strong>the</strong> killning process.<br />
L 24<br />
A novel approach to malt fl avour<br />
characterization<br />
Jens Voigt 1, Thomas Kraus-Weyermann 2,<br />
Andreas Richter 2<br />
1University of Applied Sciences Trier, Food Technology, Trier,<br />
Germany, 2Weyermann Specialty Malting, Bamberg, Germany<br />
DESCRIPTION OF TOPIC:<br />
Malt characterization is typically done only by<br />
<strong>the</strong> analysis of certain chemical or technical<br />
parameters. This does enable <strong>the</strong> brewer or<br />
malt user to assume certain behaviour in <strong>the</strong><br />
process and also some typical sensorial attributes<br />
in <strong>the</strong> fi nal product. Due to <strong>the</strong> wide<br />
range of specialty malt products it was seen<br />
necessary to develop a method to describe<br />
<strong>the</strong> fl avour characteristics of <strong>the</strong> malt be<strong>for</strong>e<br />
it actually enters <strong>the</strong> process. In a complex<br />
method a fl avour and taste description was<br />
developed describing <strong>the</strong> typically expected<br />
fl avours of malts of higher colour and o<strong>the</strong>r<br />
specifi c malts like acidifi ed, smoked or melanoidin<br />
malts. As a result a groups of diff erent<br />
fl avours were described. The fi nally ends up<br />
in <strong>the</strong> characterization in a wheel of fl avours.<br />
The methods of <strong>the</strong> tasting panel is described<br />
in this work. The overall result of this work is<br />
a tool <strong>for</strong> brewers and malt processing industries<br />
to describe sensorial attributes of certain<br />
malts and specialities.<br />
Jens Voigt<br />
Dipl.-Ing. Brewing/Beverage Technology Weihenstephan<br />
1985 PhD Weihenstephan1993<br />
Industry 1985-2004 Science TUM Weihenstephan<br />
2004-2012 Prof. Beverage Technology<br />
University Trier 2012 Publicly certifi ed Expert<br />
Brewing and Beverage Technology<br />
31 34 TH EBC CONGRESS · LUXEMBOURG 26 – 30 MAY 2013
L 25<br />
Iso<strong>the</strong>rmal and non-iso<strong>the</strong>rmal<br />
decomposition kinetics of s-methylmethionin<br />
Yarong Huang 1, Johannes Tippmann 1,<br />
Thomas Becker 1<br />
1Technische Universität München, Lehrstuhl für Brau- und<br />
Getränketechnologie, Freising, Germany<br />
DESCRIPTION OF TOPIC:<br />
Dimethylsulfi d, which has a characteristic<br />
taste and aroma of cooked celery, is one of<br />
<strong>the</strong> most studied off -fl avors in wort and beer.<br />
Although t<strong>here</strong> have been many kinetic studies<br />
conducted with diff erent wort boiling<br />
systems under iso<strong>the</strong>rmal conditions, <strong>the</strong><br />
results are diffi cult to apply to <strong>the</strong> heatingup<br />
process, in which <strong>the</strong> decomposition of smethylmethionin<br />
(SMM) also take place. And<br />
it is not easy to defi ne t=0 under iso<strong>the</strong>rmal<br />
conditions. In this study <strong>the</strong> kinetics of <strong>the</strong>rmal<br />
decomposition of SMM was studied using<br />
multiple dynamic measurements under iso<strong>the</strong>rmal<br />
and non-iso<strong>the</strong>rmal conditions. For<br />
both methods, <strong>the</strong> reaction mechanism (g(x))<br />
follows fi rst-order kinetics. The kinetic triple<br />
(Ea, A, g(x)) obtained from <strong>the</strong> non-iso<strong>the</strong>rmal<br />
method is compared with <strong>the</strong> results from<br />
iso<strong>the</strong>rmal method. In order to predict <strong>the</strong><br />
<strong>for</strong>mation of DMS and provide a better control<br />
during brewing process, a kinetic model was<br />
developed, which is applied to <strong>the</strong> heating-up<br />
process and boiling process.<br />
Yarong Huang<br />
She studied Brewing Science and Beverage<br />
Technology and graduated with a Dipl.-Eng.<br />
Degree in brewing and beverage technology<br />
from <strong>the</strong> Technical University Munich (TUM) in<br />
<strong>the</strong> year 2009. Since December 2009 she has<br />
been employed as a PhD student in <strong>the</strong> fi eld<br />
of brewing research to investigate <strong>the</strong> chemical<br />
reactions of important process markers<br />
during wort boiling processes at <strong>the</strong> TUM in<br />
Freising-Weihenstephan, chair of Brewing<br />
and Beverage Technology (Prof. Dr. Thomas<br />
Becker).<br />
L 26<br />
Fermentable and non-fermentable<br />
carbohydrates addition during<br />
brewing - eff ects on palate fullness,<br />
oxidative processes and <strong>for</strong>mation<br />
of specifi c aging compounds<br />
Niklas Ole Brandt 1, Thomas Kunz 1,<br />
Frank-Jürgen Methner 1<br />
1Technische Universität Berlin, Lab of Brewing Science,<br />
Berlin, Germany<br />
DESCRIPTION OF TOPIC:<br />
Prior researches have shown unexpected reducing<br />
potentials of diff erent carbohydrates<br />
at low pH of wort and beer. Additional investigations<br />
were carried out to get a deeper inside<br />
in <strong>the</strong> behaviour of sugars on oxidative<br />
processes during brewing and beer storage.<br />
In correlation to <strong>the</strong> reduction potentials,<br />
sugars show an opposite eff ect on oxidative<br />
processes in wort and beer. In dependence<br />
to <strong>the</strong> sugar type an acceleration of radical<br />
generation and <strong>for</strong>mation of specifi c aging<br />
compounds is detectable. The mechanisms<br />
based on <strong>the</strong> generation of specifi c intermediate<br />
Maillard products with reductone/endiol<br />
structure and <strong>the</strong>ir strong reduction capacity<br />
against metallic ions resulting in an acceleration<br />
of oxidative processes caused by Fenton<br />
reaction.<br />
In case of commonly used unfermentable sugars<br />
to get palate fullness heat stress during<br />
wort boiling is disadvantageous. To avoid heat<br />
eff ects and to use <strong>the</strong> positive eff ect on SO2<br />
<strong>for</strong>mation <strong>the</strong> addition prior fermentation is<br />
suggested.<br />
Niklas Ole Brandt<br />
Niklas Brandt has started his brewing career<br />
with an internship in a craft brewery. In 2007<br />
he began an apprenticeship as a brewer and<br />
maltster at <strong>the</strong> Brauerei Beck & Co. Bremen<br />
which he successfully completed in December<br />
2009. Since October 2009, Niklas Brandt<br />
studies Brewing and Beverage Technology at<br />
<strong>the</strong> Technische Universität Berlin, which he<br />
will fi nish in spring of 2013. Beside <strong>the</strong> study<br />
he works as a student research assistant<br />
at <strong>the</strong> Department of Biotechnology, Lab of<br />
Brewing Science.<br />
32<br />
L 27<br />
Control of cereal fl avor of beer by<br />
changing <strong>the</strong> pH and amino acid<br />
and reducing sugar composition in<br />
wort during boiling<br />
Shoko Inaba 1, Minoru Kobayashi 2,<br />
Kazuhiko Uemura 1, Masayuki Aizawa 1<br />
1Asahi Breweries Ltd., Department of Brewing Technology,<br />
Moriya-Shi Ibaraki, Japan, 2Asahi Breweries Ltd., Department<br />
of Flavor and Chemical Analysis, Moriya-Shi Ibaraki, Japan<br />
DESCRIPTION OF TOPIC:<br />
An overabundance of cereal fl avor can reduce<br />
<strong>the</strong> drinkability of high-malt beer. In contrast,<br />
<strong>the</strong> quality of low-malt beer is often reduced<br />
by an insuffi ciency of cereal fl avor. How ever,<br />
o<strong>the</strong>r than changing <strong>the</strong> quantity of malt<br />
used in <strong>the</strong> brewing, t<strong>here</strong> is little knowledge<br />
about <strong>the</strong> factors controlling <strong>the</strong> cereal fl avor.<br />
We found that cereal fl avor is controlled by<br />
changing <strong>the</strong> wort boiling condition aff ecting<br />
Maillard reaction. Beer produced using wort<br />
boiled at pH 4.0 had less cereal fl avor than<br />
that of beer produced by boiling <strong>the</strong> wort at<br />
pH 5.2. More cereal fl avor and higher levels of<br />
fl avor-active aldehydes were present in <strong>the</strong> fi -<br />
nished beer boiled at a higher pH. We also demonstrated<br />
that <strong>the</strong> characteristic cereal-like<br />
fl avor of beer was aff ected by changing <strong>the</strong><br />
composition of amino acids or reducing sugars,<br />
<strong>the</strong> Maillard reaction substrate, in wort.<br />
We conclude that <strong>the</strong> cereal fl avor in beer can<br />
be controlled by altering <strong>the</strong> pH and <strong>the</strong> composition<br />
of <strong>the</strong> wort during boiling.<br />
Shoko Inaba<br />
Received a MS degree in Agricultural Science<br />
from Tohoku University, Japan, in 2006, w<strong>here</strong><br />
she majored in Animal Microbiology. She joined<br />
Asahi Breweries, Ltd. in April 2006. Since<br />
January 2009, she has been working at department<br />
of Brewing Technology, Reserch Laboratories<br />
<strong>for</strong> Brewing.
L28<br />
Infl uence of matrix composition on<br />
body and mouthfeel - maltodextrins<br />
<strong>the</strong> body builders!<br />
Martina Gastl 1, Heinrich Rübsam 1,<br />
Georg Krebs 1, Thomas Becker 1<br />
1Technische Universität München, Freising, Germany<br />
DESCRIPTION OF TOPIC:<br />
Drinkability means a specifi c harmony and<br />
balance of a beverage. Especially body and<br />
mouthfeel aff ect <strong>the</strong> Drinkability. Mouthfeel is<br />
a product‘s physical and chemical interaction<br />
in <strong>the</strong> mouth; body is <strong>the</strong> sensation of palate<br />
fullness, including density and viscosity. It is<br />
often a challenge in beverage and beer production<br />
to create full-bodied beverages, e. g.<br />
with less alcohol or raw-material-based, without<br />
adding color or fl avor.<br />
This study documents that <strong>the</strong> intensity of<br />
body and characteristic of mouthfeel can be<br />
infl uenced signifi cant depending on <strong>the</strong> range<br />
of molecular weight distribution (MWD)<br />
of maltodextrins. T<strong>here</strong><strong>for</strong>e diff erent matrix<br />
compositions were per<strong>for</strong>med by variation<br />
of <strong>the</strong> range of MWD (spiking trials). A human<br />
taste panel was trained to describe <strong>the</strong>ir sensations<br />
with standardised terminology and a<br />
uni<strong>for</strong>m developed taste schema. The eff ects<br />
were tested and confi rmed using diff erent<br />
beer types (alcohol free, low-alcohol, raw material<br />
based and all-malt beer).<br />
Martina Gastl<br />
Martina Gastl apprenticed as a brewer and<br />
maltster from 1994 to 1996 in Klosterbrauerei<br />
Andechs, Germany. She studied brewing<br />
and beverage technology at <strong>the</strong> Technische<br />
Universität München-Weihenstephan, Germany<br />
and competed her ph. D. from 2002 to<br />
2006; since 2010 she works on her postdoctoral<br />
lecture qualifi cation.She is currently assistant<br />
professor and head of <strong>the</strong> laboratory<br />
as well as <strong>the</strong> raw material and beverage design<br />
research group at <strong>the</strong> TUM Lehrstuhl für<br />
Brau- und Getränketechnologie.<br />
L 29<br />
RNAseq analysis of Saccharomyces<br />
cerevisiae‘s response to contamination<br />
by Lactobacillus plantarum<br />
Trevor G Phister 1, Vanessa Pittet 2,<br />
Barry Ziola 2<br />
1University of Nottingham, Loughborough, United Kingdom,<br />
2University of Saskatchewan, Department of Pathology and<br />
Laboratory Medicine, Saskatoon, Canada<br />
DESCRIPTION OF TOPIC:<br />
Saccharomyces and lactobacilli are often<br />
found toge<strong>the</strong>r in alcoholic fermentations<br />
including beer, whisky, and wine. Lactobacilli<br />
are often considered contaminants in brewing<br />
fermentations, brought in through <strong>the</strong> use of<br />
a contaminated starter culture or serial repitching<br />
of yeasts. On one hand, lactobacilli can<br />
contribute positively to wine and whisky fermentations<br />
by improving <strong>the</strong> fl avour profi le<br />
of <strong>the</strong> fi nal product. They can also be problematic,<br />
however, by creating stuck or sluggish<br />
fermentations, as well as off fl avours.<br />
In this study, we examined <strong>the</strong> eff ects of <strong>the</strong><br />
presence of lactobacilli on gene expression in<br />
Saccharomyces. RNA-sequencing was per<strong>for</strong>med<br />
on a mixed S. cerevisiae and L. plantarum<br />
culture at exponential growth and gene<br />
expression was compared to that of unmixed<br />
cultures at <strong>the</strong> same stage of growth. In our<br />
model system, we found that that Saccharomyces<br />
up-regulated xenobiotic transporter<br />
activity, w<strong>here</strong>as pathways involved in purine<br />
syn<strong>the</strong>sis were down regulated.<br />
Trevor G Phister<br />
Dr. Phister is a Senior Research Fellow in <strong>the</strong><br />
brewing science program at <strong>the</strong> University of<br />
Nottingham. Previously he worked in <strong>the</strong> food<br />
science department at North Carolina State<br />
University. At Nottingham, he is currently<br />
working on stress tolerance in ethanol producing<br />
yeasts. His research interest include,<br />
detection and identifi cation of spoilage microbes<br />
in fermented foods, <strong>the</strong> role of yeasts and<br />
bacteria in fl avour <strong>for</strong>mation and <strong>the</strong> interaction<br />
between yeasts and bacteria in fermentations.<br />
L30<br />
The eff ect of successive use of<br />
lager yeast starter culture on sugar<br />
uptake dynamics from wort<br />
Nataša Kočar 1, Matej Oset 1, Iztok J Košir 2,<br />
Peter Raspor 3<br />
1Pivovarna Laško d.d., Laško, Slovenia, 2Slovenian Institute<br />
of Hop Research and Brewing, Žalec, Slovenia, 3Biotechnical<br />
Faculty of Ljubljana, Departement of Biotechnology, Microbiology<br />
and Food Safety, Ljubljana, Slovenia<br />
DESCRIPTION OF TOPIC:<br />
The fermentation per<strong>for</strong>mance of brewing<br />
yeast has a strong impact on beer quality.<br />
Because of successive use of single yeast<br />
starter culture and <strong>the</strong> nature of beer production<br />
process, yeasts are exposed to stress<br />
factors. In this study we monitored fermentation<br />
process during twelve beer productions.<br />
The work was done at industrial scale, with<br />
constant environmental conditions. Monitoring<br />
starter culture viability during successive<br />
fermentation we concluded that <strong>the</strong><br />
viability and fi tness of yeast is not reduced.<br />
Since we focused our study on <strong>the</strong> impact of<br />
successive exploitation of a single yeast starter<br />
culture on sugar uptake dynamics from<br />
wort, we can observe interesting dynamics of<br />
sugar uptake, which is improved during <strong>the</strong><br />
time course in particular in uptake of DP2 and<br />
DP3 sugars. The conclusions of our study also<br />
suggests that we should consider <strong>the</strong> possibility<br />
that fur<strong>the</strong>r exploitation can be continued<br />
since <strong>the</strong> yeast potential is fully developed after<br />
twelve successive ba<strong>the</strong>s.<br />
Nataša Kočar<br />
Nataša Kočar is working in a brewery Pivovarna<br />
Laško d.d., as head of quality control department.<br />
After she graduated from microbiology<br />
at Univerity of Ljubljana, she continued<br />
master‘s degree study in biotechnology at <strong>the</strong><br />
Biotechnical Faculty, University of Ljubljana,<br />
which she completed by <strong>the</strong> presentation of<br />
master <strong>the</strong>sis: The imapct of serial repitching<br />
of S. pastorianus on sugar uptake dymanics<br />
from wort. At <strong>the</strong> moment she is PhD student<br />
in biosciences, in <strong>the</strong> fi eld of food processing.<br />
33 34 TH EBC CONGRESS · LUXEMBOURG 26 – 30 MAY 2013
L 31<br />
A comparison of fermentation<br />
by-products to determine beer<br />
maturation regardless of style and<br />
processing parameters<br />
Urs Wellhoener 1<br />
1The Boston Beer Company, Breinigsville, United States<br />
DESCRIPTION OF TOPIC:<br />
Over 20+ styles were investigated in 2 breweries<br />
<strong>for</strong> <strong>the</strong>ir behavior during storage. The<br />
study included beers that were krausened,<br />
non-krausened, centrifuged, non-centrifuged,<br />
dry hopped, and non-dry hopped. Additionally<br />
<strong>the</strong> beer underwent various storage conditions,<br />
like temperature and tank geometry.<br />
Samples were analyzed by an external lab <strong>for</strong><br />
23 diff erent fermentation by-products. The<br />
goal was to identify <strong>the</strong> key makers <strong>for</strong> maturation.<br />
Finding <strong>the</strong> right maturation times<br />
is not only crucial <strong>for</strong> capacity related questions,<br />
but <strong>for</strong> beer quality as well with regard<br />
to overaging. The results were investigated<br />
independent of style and technology to evaluate<br />
<strong>for</strong> common maturation parameter. Deviations<br />
from commonality were investigated.<br />
Not only did this study identify key parameters<br />
among various beer styles <strong>for</strong> maturation.<br />
The results also helped to fi nding <strong>the</strong> right<br />
lab equipment to determine when a beer is<br />
mature.<br />
Urs Wellhoener<br />
Urs Wellhoener is <strong>the</strong> corporate manager<br />
<strong>for</strong> Yeast&Fermentation <strong>for</strong> <strong>the</strong> Boston Beer<br />
Company since 2007. After graduation as brewer<br />
and maltster in 1993 he received a Dipl.-<br />
Eng. degree from <strong>the</strong> Technische Universität<br />
München-Weihenstephan (TUM) in 1999. Between<br />
2000 and 2007 Wellhoener was a scientifi<br />
c assistant and doctorate at <strong>the</strong> Chair of<br />
Brewing Technology II at <strong>the</strong> Weihenstephan<br />
Center of Food and Life Sciences, Technische<br />
Universität München-Weihenstephan (TUM).<br />
L 32<br />
Use and maintenance of synchronous<br />
yeast cultures<br />
John D Sheppard 1, Johnathon B Layfi eld 1,<br />
Lucas R Vann 1<br />
1North Carolina State University, Food, Bioprocessing &<br />
Nutrition Sciences, Raleigh, United States<br />
DESCRIPTION OF TOPIC:<br />
Batch to batch variability in <strong>the</strong> fermentation<br />
per<strong>for</strong>mance of yeast cultures is a common<br />
problem in breweries. One cause of this variability<br />
can be a change in <strong>the</strong> characteristics of<br />
<strong>the</strong> yeast population, as a result of metabolic<br />
adaptation, stress responses and <strong>the</strong> natural<br />
aging process. A wide distribution of phenotypic<br />
responses within <strong>the</strong> yeast population can<br />
lead to increased variability in <strong>the</strong> observed<br />
rate of wort attenuation and fl avour profi le.<br />
Thus, a yeast management strategy that is<br />
designed to narrow <strong>the</strong> distribution of responses<br />
within <strong>the</strong> population should lead to<br />
increased uni<strong>for</strong>mity in fermentation per<strong>for</strong>mance.<br />
A system <strong>for</strong> developing and maintaining<br />
synchronous yeast populations has been<br />
developed, which has been shown to provide<br />
a high level of metabolic control and results in<br />
an increase in <strong>the</strong> consistency of consecutive<br />
fermentation batches.<br />
John D Sheppard<br />
Professor of Food, Bioprocessing and Nutrition<br />
Sciences at North Carolina State University<br />
since 2006, previously a professor at McGill<br />
University in Montreal, Canada. Engineer and<br />
scientist that specializes in research into fermentation<br />
and bioprocess control, utilizing a<br />
2.5 bbl research brewery.<br />
34<br />
L 33<br />
MALDI-TOF MS imaging of barley<br />
seeds during malting<br />
Karin Gorzolka 1, Karsten Niehaus 1<br />
1Bielefeld University, Proteome and Metabolome Research,<br />
Bielefeld, Germany<br />
DESCRIPTION OF TOPIC:<br />
MALDI mass spectrometry imaging (MSI) is<br />
a new approach to localize a wide range of<br />
compounds in a sample section. We applied<br />
MALDI MSI on barley during malting in two<br />
seed orientations: longitudial and transversal.<br />
More than 100 signals (e.g. fatty acids, oligosaccharides)<br />
were detected in <strong>the</strong> global<br />
spectrum. The power of this approach was<br />
<strong>the</strong> description of unique compounds in even<br />
small seed tissues (e.g. aleuron layer) and<br />
fur<strong>the</strong>r <strong>the</strong> development of compound distribution<br />
and detection of new signals during<br />
<strong>the</strong> ongoing malting process.<br />
MALDI imaging was supplemented by <strong>the</strong><br />
analysis of hydrophilic compounds (gas chromatography<br />
- mass spectrometry) and raster<br />
electron microscopy.<br />
This unique study provides a new background<br />
<strong>for</strong> <strong>the</strong> understanding of <strong>the</strong> malting process.<br />
The enhanced knowledge of compound localization<br />
and <strong>the</strong> analysis of changes in <strong>the</strong><br />
seeds during germination will certainly serve<br />
to optimize process management and t<strong>here</strong>by<br />
to enhance malt quality.<br />
Karin Gorzolka<br />
Karin Gorzolka studied Biology in Bielefeld,<br />
Germany, from 2002 to 2009 with special focus<br />
on molecular biology. After receiving her<br />
Diploma degree she started working on her<br />
PhD <strong>the</strong>sis about <strong>the</strong> characterization of <strong>the</strong><br />
barley malting process by protein and metabolite<br />
analysis, which she will fi nish in 2013.
L 34<br />
Brewing properties of new German<br />
hop varieties<br />
Adrian Forster 1<br />
1HVG e.G., Wolnzach, Germany<br />
DESCRIPTION OF TOPIC:<br />
After registration, new hop varieties are<br />
mostly evaluated favourably regarding <strong>the</strong>ir<br />
brewing properties although systematic trials<br />
are missing. This is also <strong>the</strong> case <strong>for</strong> <strong>the</strong> four<br />
new German breedings, <strong>the</strong> so called “fl avour<br />
hops”.<br />
Crop 2012 is <strong>the</strong> fi rst crop to provide new hop<br />
varieties from mature plants. These can be<br />
used to conduct brewing trials against established<br />
varieties with brews of 2 hl and include<br />
<strong>the</strong> following areas:<br />
1. General brewing properties are analysed,<br />
particularly <strong>the</strong> infl uence on bitterness and<br />
taste of <strong>the</strong> beers.<br />
2. Late dosages during wort boiling are intended<br />
to show <strong>the</strong> qualifi cations of <strong>the</strong> varieties<br />
<strong>for</strong> a late-hop-aroma.<br />
3. The characteristics of varieties in dry-hopped<br />
beers are to be evaluated as well.<br />
Apart from analytical characterisation of all<br />
hop-relevant ingredients in beers, a sensorial<br />
evaluation will be done, too. Here, a focus<br />
will also be on <strong>the</strong> stability of late and dry hop<br />
aroma.<br />
Adrian Forster<br />
Adrian Forster (born in 1942) attended <strong>the</strong><br />
Technical University Munich / Weihenstephan<br />
(1966 brewing engineer) and obtained a Ph.D.<br />
degree in brewing science in 1972. 1969 -<br />
1973 scientist 1973 - 2003 managing director<br />
of <strong>the</strong> world‘s leading hop extraction and hop<br />
pellet plant with responsibilities in research.<br />
Forster has published hop-related topics extensively<br />
and is currently a hop consultant.<br />
L 35<br />
Fusarium and mycotoxins in barley<br />
from monitoring to risk management<br />
in France<br />
Alain Froment 1<br />
1Syngenta, Guyancourt, France<br />
DESCRIPTION OF TOPIC:<br />
Because of possible harmfull eff ects mycotoxins<br />
are more and more monitored and regulated<br />
all around <strong>the</strong> world. Fusarium may<br />
be toxins producer but also impacting <strong>the</strong><br />
grain quality. In order to estimate <strong>the</strong> level of<br />
contamination in barley, Syngenta organized<br />
in France a large fi eld survey since 2005. Agronomic<br />
practices and climatic data of more<br />
than 2500 farmer fi elds were listed. Mycotoxins<br />
were analysed by HPLC on grain samples<br />
representative of each fi eld. Main types of<br />
Fusarium were quantifi ed on some samples<br />
of grain. This database allowed to assess <strong>the</strong><br />
agronomic risk management since 2008. A<br />
<strong>for</strong>ecasting model including climatic data is<br />
under development to predict <strong>the</strong> risk of <strong>the</strong><br />
year <strong>for</strong> T2-HT2 toxins be<strong>for</strong>e harvesting.<br />
Alain Froment<br />
Agronomist Food Chain Manager.Syngenta<br />
France<br />
L 36<br />
Development of a rapid and sensitive<br />
multi-residue method to determine<br />
pesticides in hops and hop<br />
products<br />
Martin Biendl 1<br />
1Hopsteiner HHV GmbH, Mainburg, Germany<br />
DESCRIPTION OF TOPIC:<br />
Hop growers carefully balance between combating<br />
pests and pathogens and producing a<br />
crop that can pass <strong>the</strong> import tolerances or<br />
maximum residue limits (MRLs) established<br />
by <strong>the</strong> various countries to which hops are exported.<br />
To control MRL´s <strong>the</strong> offi cial standard<br />
methods <strong>for</strong> analysing diff erent crops cannot<br />
be applied to hops due to <strong>the</strong> more complex<br />
matrix of this plant (e.g. high content of resins).<br />
Modifi ed residue methods <strong>for</strong> hops are<br />
rarely published. In <strong>the</strong> current study, a suitable<br />
rapid method was developed to determine<br />
<strong>the</strong> residue levels of more than 40 pesticides<br />
in hop cones and hop products (pellets, extracts).<br />
This method utilizes liquid extraction,<br />
solid phase extraction, and fi nally gas chromatography<br />
(GC) or liquid chromatography<br />
(LC) each combined with mass spectrometry<br />
(MS). In hops and hop pellets a limit of quantifi<br />
cation (LOQ) of 0.1 mg/kg (ppm) could be<br />
achieved <strong>for</strong> all active ingredients. In hop extracts<br />
<strong>the</strong> LOQ´s are a factor of 2-5 higher.<br />
Martin Biendl<br />
Martin Biendl received a Ph.D. degree in organic<br />
chemistry from Regensburg University<br />
in 1990. He is head of <strong>the</strong> R&D/Analytical<br />
Department at <strong>the</strong> German branch of <strong>the</strong><br />
Hopsteiner group. His research experience is<br />
in <strong>the</strong> fi eld of hop-related needs <strong>for</strong> <strong>the</strong> brewing<br />
industry and beyond. Since 2001 he is <strong>the</strong><br />
chairman of <strong>the</strong> Hops Subcommittee in <strong>the</strong><br />
EBC Analysis Committee. As EBC representative<br />
he is also co-chairman of <strong>the</strong> International<br />
Hop Standards Committee.<br />
35 34 TH EBC CONGRESS · LUXEMBOURG 26 – 30 MAY 2013
L 37<br />
Effi cient processing methods <strong>for</strong><br />
increasing yield when using hop<br />
products <strong>for</strong> wort production<br />
Ludwig Scheller 1<br />
1GEA <strong>Brewery</strong> Systems GmbH, Technology, R&D, Kitzingen,<br />
Germany<br />
DESCRIPTION OF TOPIC:<br />
Alpha acids are <strong>the</strong> valuable constituents<br />
contained in <strong>the</strong> yellow lupulin glandsof <strong>the</strong><br />
hop umbel. During wort boiling <strong>the</strong> ring structure<br />
of <strong>the</strong> alpha acids will be changed from<br />
hexagonal to pentagonal and <strong>the</strong> bitter acids<br />
can be solved in <strong>the</strong> wort. This is a precondition<br />
to create <strong>the</strong> bitterness profi le in <strong>the</strong> beer<br />
desired by brewers.<br />
The project objective is <strong>the</strong> increasing of <strong>the</strong><br />
alpha acid conversion rate during wort boiling<br />
when hop extract is used. One possibility to<br />
realize a better conversion rate is <strong>the</strong> reduction<br />
of <strong>the</strong> droplet size of hop extract. The applied<br />
technology was tested in laboratory and<br />
pilot plant scale be<strong>for</strong>e doing trials in industrial<br />
scale. The results of <strong>the</strong>se trails are promissing.<br />
The technology improves <strong>the</strong> yield<br />
of bitter substances signifi cantly by dosing<br />
<strong>the</strong> homogenized and isomerized hop extract<br />
suspension into <strong>the</strong> hot wort.<br />
The savings can be summarized in reduced<br />
raw material cost and lower energy cost by<br />
shorter boiling time.<br />
Ludwig Scheller<br />
Dr. Ing. Ludwig Scheller, team member of <strong>the</strong><br />
department Technology and R&D of GEA <strong>Brewery</strong><br />
Systems GmbH<br />
L 38<br />
Classifi cation of renewable fi lter<br />
aids - an opportunity to control <strong>the</strong><br />
permeability of <strong>the</strong> fi lter cake<br />
Alexander Scheidel 1, Jens Voigt 2, Petra<br />
Först 1, Karl Sommer 1<br />
1TU München, Chair of Process Engineering of Disperse<br />
Systems, Freising, Germany, 2University of Applied Science,<br />
Trier, Germany<br />
DESCRIPTION OF TOPIC:<br />
Precoat fi ltration with fi lter aids is <strong>the</strong> most<br />
common process to eliminate haze causing<br />
materials out of <strong>the</strong> maturated beer. For a satisfactory<br />
use of renewable fi lter aids it is important<br />
to be able to change <strong>the</strong> composition<br />
and <strong>the</strong> permeability of <strong>the</strong> fi lter cake. A separation<br />
process with a hydrocyclone classifi<br />
er was developed to receive diff erent coarse<br />
grades of fi lter aid fractions with diff erent fi lter<br />
characteristics. The parameters of <strong>the</strong> separation<br />
process can be changed <strong>for</strong> diff erent<br />
requirements on <strong>the</strong> fi lter cake permeability.<br />
This process enables <strong>the</strong> brewery to change<br />
<strong>the</strong> mixture of <strong>the</strong> fi lter aid fractions in a desired<br />
range. A variable use of precoating and<br />
body feed of renewable fi lter aids is necessary<br />
<strong>for</strong> diff erent beer types or during fi ltration<br />
problems. The classifi cation by hydrocyclones<br />
is a possible process to substitute kieselguhr<br />
with renewable fi lter aids in existing plants<br />
and <strong>for</strong> a sustainable fi ltration process.<br />
Alexander Scheidel<br />
Alexander Scheidel graduated in 2011 with<br />
a diploma degree in brewing and beverage<br />
technology at TU München in Weihenstephan.<br />
The focus of his studies was on process engineering<br />
and brewing technology. Since 2011<br />
he works as PhD student at <strong>the</strong> chair of process<br />
engineering of disperse systems in Weihenstephan.<br />
36<br />
L 39<br />
Validity of <strong>the</strong> laboratory fi ltration<br />
as a prediction of <strong>the</strong> fi lterability of<br />
beer<br />
Michael Kupetz 1, Martin Zarnkow 1,<br />
Thomas Becker 1<br />
1TUM Weihenstephan, Lehrstuhl für Brau- und Getränketechnologie,<br />
Freising, Germany<br />
DESCRIPTION OF TOPIC:<br />
A prediction of <strong>the</strong> fi lterability of beer using<br />
analytical methods is still not possible. The<br />
ingredients contained in <strong>the</strong> rough beer cause<br />
reversible and irreversible blockages of fi lters<br />
and fouling of <strong>the</strong> surface. A characterization<br />
of <strong>the</strong> fi lter media is essential <strong>for</strong> predicting<br />
<strong>the</strong> fi lterability. Interactions between <strong>the</strong> fi lter<br />
media and <strong>the</strong> suspension to be fi ltered have<br />
a great impact on <strong>the</strong> clarifi cation. Common<br />
methods <strong>for</strong> prognosis are membrane or precoat<br />
laboratory fi ltration. On an automated<br />
system, both membrane- (e.g. Essertest), as<br />
well as precoat fi ltration (Raibletest) can be<br />
per<strong>for</strong>med. By varying <strong>the</strong> fi lter aid fi ltration<br />
problems can be detected quickly and easily.<br />
A conversion can provide an approximate<br />
prediction <strong>for</strong> <strong>the</strong> industrial scale. So, fi ltration<br />
processes can be characterized with <strong>the</strong><br />
help of simple tests.<br />
Michael Kupetz<br />
Michael Kupetz graduated from Technische<br />
Univeristät Müchnen in 2011 as a degreed<br />
engineer <strong>for</strong> brewing sciences and beverage<br />
technology. In 2012 he started his doctoral<br />
<strong>the</strong>sis with Prof. Thomas Becker at <strong>the</strong> institute<br />
of brewing science and beverage technology.<br />
His research project is <strong>the</strong> ‚Comparative<br />
identifi cation of inhibitory substances in <strong>the</strong><br />
membrane and diatomaceous earth fi ltration<br />
of beer‘
L 40<br />
Permeation and migration behavior<br />
of polyethylene terephthalate polymer,<br />
PET/PA polymer blends and<br />
SiOx coated PET bottles<br />
Sven Sängerlaub 1, Kajetan Müller 1,<br />
Frank Welle 1<br />
1Fraunhofer Institute <strong>for</strong> Process Engineering and Packaging<br />
IVV, Freising, Germany<br />
DESCRIPTION OF TOPIC:<br />
Polyethylene terephthalate (PET) bottles are<br />
widely used <strong>for</strong> beverages. Oxygen-sensitive<br />
beverages, however, often require <strong>the</strong> use of<br />
additional barrier materials or coatings or<br />
oxygen-scavenging additives incorporated<br />
into <strong>the</strong> PET material. The proposed presentation<br />
will summarize <strong>the</strong> results of recent<br />
research activities about <strong>the</strong> permeation and<br />
sorption behaviour of diff erent functional<br />
barriers materials <strong>for</strong> PET bottles against<br />
oxygen, carbon dioxide, acetaldehyde and different<br />
organic compounds. The content of <strong>the</strong><br />
presentation includes <strong>the</strong> following issues:<br />
a) Barrier per<strong>for</strong>mance of inorganic vs polymeric<br />
vs active parrier layers,<br />
b) Temperature dependence on mass transport,<br />
c) Diff usion and sorption behaviour of diff erent<br />
gases and vapours,<br />
d) Recyclability of PET bottles with additional<br />
barrier.<br />
Sven Sängerlaub<br />
Sven Sängerlaub, born in 1978, studied Packaging<br />
Technology at <strong>the</strong> Leipzig University<br />
of Applied Sciences (HTWK). Since 2004, he is<br />
working at Fraunhofer IVV in <strong>the</strong> fi eld of active<br />
and intelligent packaging, with focus on oxygen<br />
scavengers, humidity regulation,<br />
barrier testing and humidity indicators. He is<br />
actively involved in preparing project proposals<br />
and seeks investment & co-investment<br />
by industry and funding agencies. He is chairman<br />
of <strong>the</strong> DIN committee „Active Packaging/<br />
Oxygen Scavengers“.<br />
L 41<br />
Establishment of a new beer canning<br />
process based on dew-point<br />
temperature fi lling technology<br />
Isamu Otake 1, Tatsuhiko Sato 2<br />
1Asahi Breweries, Ltd., Production Technology Center R&D<br />
Promotion Offi ce Ibaraki <strong>Brewery</strong>, Moriya Ibaraki, Japan,<br />
2Asahi Breweries, Ltd., Production Technology Center R&D<br />
Promotion Offi ce Suita <strong>Brewery</strong>, Suita Osaka, Japan<br />
DESCRIPTION OF TOPIC:<br />
Our packaging process of draft can beer is<br />
as follows: <strong>the</strong> product is sent to a fi ller, and<br />
cans are fi lled and sealed maintaining a low<br />
temperature of about 1°C. Next, <strong>the</strong> temperature<br />
is raised to ≥20°C in a warmer, and <strong>the</strong><br />
cans are packed. The warmer is a machine<br />
that showers <strong>the</strong> product with warm water to<br />
prevent dew condensation. But, <strong>the</strong> warmer<br />
has problems, including a large <strong>the</strong>rmal load<br />
because of low heat exchange effi ciency, and<br />
requiring much space. We aimed to develop a<br />
new canning process that producing a draft<br />
beer without warmer. We examined two measures:<br />
raising <strong>the</strong> temperature of <strong>the</strong> beer<br />
using a heat exchanger be<strong>for</strong>e fi lling, and<br />
fi lling <strong>the</strong> beer at <strong>the</strong> dew-point temperature.<br />
Using a heat exchanger improved <strong>the</strong> heat<br />
transfer effi ciency. We controlled <strong>the</strong> fi lling<br />
speed to achieve foaming inhibition and now<br />
we can fi ll beer at temperature ranging from<br />
0 to 22°C. With <strong>the</strong>se methods, it has become<br />
possible to design new canning draft beer lines<br />
that save energy and space.<br />
Isamu Otake<br />
Isamu Otake joined Asahi Breweries Ltd.<br />
in 2000. 2001 to 2004 he worked in <strong>the</strong> packaging<br />
section at Nagoya <strong>Brewery</strong> as a stuff<br />
member of <strong>the</strong> can lines, focusing on quality<br />
management and operational effi ciency. 2005<br />
to 2007 he worked in <strong>the</strong> packaging section<br />
at Ibaraki <strong>Brewery</strong> as a line manager of bottle<br />
lines and keg lines. Currently, He is project<br />
engineer <strong>for</strong> promotion of new packaging<br />
technology at Production Technology Center,<br />
in Ibaraki.<br />
L 42<br />
Time-Temperature Integrator <strong>for</strong><br />
<strong>the</strong> fl ash pasteurization<br />
Jan Schneider 1, Anna Dammann 1, Knut<br />
Schwarzer 1, Ulrich Müller 1<br />
1University of Applied Sciences Ostwestfalen-Lippe, Institute<br />
of Food Technology NRW, Lemgo, Germany<br />
DESCRIPTION OF TOPIC:<br />
A chemical Time-Temperature Integrator (TTI)<br />
is expected to measure more accurate, cheaper<br />
and faster than conventional microbiological<br />
count reduction tests <strong>for</strong> <strong>the</strong> validation<br />
of fl ash pasteurizes. The acidic hydrolysis of<br />
sucrose was investigated as a TTI. The calibration<br />
of <strong>the</strong> chemical reaction was carried<br />
out order to fi nd <strong>the</strong> suitable parameters<br />
(acid and sucrose concentrations) with two<br />
methods, iso<strong>the</strong>rmal and non-iso<strong>the</strong>rmal. As<br />
long as <strong>the</strong> process temperature is constant<br />
- as nearly in pasteurizers holding tubes -<br />
t<strong>here</strong> is no obstacle <strong>for</strong> <strong>the</strong> transfer of <strong>the</strong> TTI<br />
results into microbiological eff ects even if <strong>the</strong><br />
activation energies of <strong>the</strong> <strong>the</strong>rmal death kinetics<br />
of microorganism are higher than those<br />
chemical reactions. With <strong>the</strong> residence time<br />
distributions in <strong>the</strong> holding tubes even <strong>the</strong> PU<br />
can be derived from <strong>the</strong> TTI. The verifi cation<br />
with temperature measurements and a comparison<br />
with a microbiological count reduction<br />
test in a fl ash pasteurizer is part of <strong>the</strong><br />
investigation.<br />
Jan Schneider<br />
Studies: TU Munich Weihenstephan (1991-<br />
1996), phD-Student / assistant TU Munich<br />
(1997-2001). Industry: Pall corp. (2001-<br />
2002), Novartis, Austria (2002-2003), Head<br />
of <strong>the</strong> research institute of plant equipment<br />
and packaging technology at <strong>the</strong> VLB, Berlin<br />
(2003-2006). University Lec-tureship: TU<br />
Munich (1997-2001), TU Berlin (2003-2005)<br />
Professorship: University of Applied Sciences<br />
Ostwestfalen-Lippe (since 2006). Co-founder<br />
and board member of <strong>the</strong> Institute of Food<br />
Technology ILT.NRW since 2011.<br />
37 34 TH EBC CONGRESS · LUXEMBOURG 26 – 30 MAY 2013
L 43<br />
Study of diff erent rice varieties and<br />
malting parameters <strong>for</strong> <strong>the</strong> production<br />
of a saccharifying rice malt<br />
Giuseppe Perretti 1, Ombretta Marconi 1,<br />
Gian Franco Regnicoli 1, Heidi Mayer 1,<br />
Mauro Fanari 1, Paolo Fantozzi 1<br />
1University of Perugia, Italian Brewing Research Centre<br />
(CERB), Perugia, Italy<br />
DESCRIPTION OF TOPIC:<br />
Aims: Rice is a gluten free raw material suitable<br />
<strong>for</strong> <strong>the</strong> production of food, beer and cereal<br />
based beverages. The aims of this work were<br />
to obtain a saccharifying rice malt and to investigate<br />
its quality.<br />
Methods: The malting attitude of nine Italian<br />
rice varieties was investigated using a micromalting<br />
system. Rices and rice malts quality<br />
was evaluated by <strong>the</strong> A-EBC methods.<br />
Results: A malting program able to produce a<br />
saccharifying rice malt was successfully developed.<br />
The process includes a low temperature<br />
and long time kilning. To evaluate <strong>the</strong><br />
quality of <strong>the</strong> rice malt, a specifi c congress<br />
wort was optimized, and <strong>the</strong> rice malt with or<br />
without acrospires and rootlets was characterized.<br />
Conclusions: The results highlight <strong>the</strong> role<br />
of mild kilning conditions to preserve <strong>the</strong><br />
rice enzymes. The rice malt congress wort<br />
saccharifi ed completely. The optimized rice<br />
malt can be used as main ingredient <strong>for</strong> <strong>the</strong><br />
production of a high quality and gluten free<br />
product, as beer, without enzyme adjuncts.<br />
Giuseppe Perretti<br />
Born in Brindisi (Italy) in 1968. Master-degree<br />
in Agriculture (1994) and Doctorate in Food<br />
Biotechnology (2003). Since 2004 Assistant<br />
Professor at Faculty of Agriculture, Department<br />
of Economic and Food Sciences. Since<br />
2007 Vice-Director and Scientifi c Responsable<br />
of <strong>the</strong> Italian Brewing Research Centre<br />
(CERB). His main research activities deal with:<br />
brewing science and technology; mild technologies;<br />
quality control and management<br />
systems. He is author of about 100 scientifi c<br />
publications.<br />
L 44<br />
Creating novel raw materials <strong>for</strong><br />
beer production - development<br />
of LOX-less barley and <strong>the</strong> future<br />
strategy<br />
Hisao Kuroda 1, Chizuru Sato 1, Tatsuro<br />
Shigyo 1<br />
1Sapporo Breweries Ltd., Frontier Laboratories of Value<br />
Creation, Yaizu, Japan<br />
DESCRIPTION OF TOPIC:<br />
One of <strong>the</strong> most important issues in modern<br />
brewing is to control fl avor- and foam-stability<br />
of beer. Trans-2-nonenal and trihydroxyoctadecenoic<br />
acids, oxidized lipids produced by<br />
barley lipoxygenase (LOX), reduce <strong>the</strong>se quality.<br />
To overcome this issue, we developed LOXless<br />
barley and proved that beer brewed with<br />
this barley had less cardboard fl avor, improved<br />
beer foam and less astringency.<br />
In beer, t<strong>here</strong> are much more fl avor compounds<br />
to be studied. Recent progress of <strong>the</strong><br />
science of taste enabled us to identify fl avor<br />
compounds by taste receptors. If we can apply<br />
this strategy to screen raw materials, we<br />
may produce beer with superior quality having<br />
more or less of target fl avors. We are<br />
focusing on one of receptors, TRP channel,<br />
which expresses on tongue, mouth and throat<br />
and perceives sensation produced by food<br />
compounds. In this lecture, we will show TRP<br />
channel agonists in beer raw materials, which<br />
is expected to have eff ects on <strong>the</strong> smoothness,<br />
palate-fullness or body of beer.<br />
Hisao Kuroda<br />
Hisao Kuroda received M. Sc. and Ph. D. in<br />
science from <strong>the</strong> Graduate School of Science,<br />
Nagoya University in 1989 and 2006, respectively.<br />
He joined Plant Bioengineering Research<br />
Laboratories, SAPPORO BREWERIES<br />
LTD. as a Research Scientist in 1989, and now<br />
he is working on analysis of fl avour compounds<br />
in beer and beer raw materials using<br />
taste receptor assays as a Chief Biochemist in<br />
Frontier Laboratories of Value Creation, SAP-<br />
PORO BREWERIES LTD.<br />
38<br />
L 45<br />
New opportunities <strong>for</strong> high attenuated<br />
beers demonstrated by a<br />
<strong>the</strong>rmostable glycoamylase from<br />
Penicillium oxalicum<br />
Jens Eklöf 1, Mette Bhatia Frederiksen 1,<br />
Hans Peter Heldt-Hansen 1, Niels Elvig 1,<br />
Chee- Leong Soong 1, Shiro Fukuyama 2,<br />
Keiichi Ayabe 2<br />
1Novozymes A/S, Brewing Application Department, R&D,<br />
Bagsvaerd, Denmark, 2Novozymes A/S, Biotechnology R&D,<br />
Chiba-Shi, Japan<br />
DESCRIPTION OF TOPIC:<br />
The light lager beer style (high attenuated<br />
beer, >75% RDF) is commonly made using<br />
malt combined with a corn or rice adjunct<br />
source (liquid or solid). Solid adjunct is being<br />
processed in a cereal cooker prior to blending<br />
with <strong>the</strong> malt mash followed by saccharifi cation<br />
at 64-66°C. This paper will describe <strong>the</strong><br />
benefi ts obtained by a <strong>the</strong>rmostable glycoamylase<br />
from Penicillium oxalicum (Po-AG)<br />
with optimum at 70°C and ~80% residual activity<br />
at 78°C (pH 6). The traditional Aspergillus<br />
niger glycoamylase <strong>for</strong> high attenuated beer<br />
has optimum at 65°C with only ~5% residual<br />
activity at 78°C. Laboratory mashing trials<br />
with Po-AG enabled I) continued saccharifi cation<br />
during late mashing steps and lautering<br />
leading to mash time reduction, II) optimized<br />
wort sugar profi les benefi cial <strong>for</strong> certain<br />
yeast strains, III) faster wort recovery and IV)<br />
avoiding cereal cooking by infusion mashing.<br />
Po-AG provides unique benefi ts <strong>for</strong> high attenuated<br />
beers which can be of great benefi t<br />
to <strong>the</strong> brewing industry.<br />
Jens Eklöf<br />
Name: Jens Eklöf Contact details: jeeq@novozymes.com<br />
- Current and relevant previous<br />
experience:2012-present, Scientist at Novozymes<br />
A/S-Food and Brewing R&D department.<br />
2011-2012, Post-doctoral researcher at<br />
<strong>the</strong> University of British Columbia-Carbohydrate<br />
enzymology. 2011, Ph.D. student at <strong>the</strong><br />
Royal Institute of Technology-Ph.D. student<br />
position with Prof. H. Brumer at <strong>the</strong> Division<br />
of Glycoscience, Royal Institute of Technology,<br />
Stockholm, Sweden. 2006, Master of Science<br />
in Engineering at <strong>the</strong> Royal Institute of Technology.
L 46<br />
Mashing with direct steam heating<br />
and without mechanical agitation<br />
Matthias Kern 1, Johannes Tippmann 1,<br />
Thomas Becker 1<br />
1Technische Universität München, Freising, Germany<br />
DESCRIPTION OF TOPIC:<br />
To produce high quality beer, <strong>the</strong> brewhouse<br />
operations are important intermediate steps.<br />
A closer look on <strong>the</strong> mashing systems shows,<br />
that nearly all modern systems have various<br />
disadvantages, such as oxygen pick-up,<br />
poor heat transfer rates and increased shear<br />
<strong>for</strong>ces.<br />
Next to <strong>the</strong> problems mentioned above, aims<br />
of <strong>the</strong> study are <strong>the</strong> reduction of mechanical<br />
components (e.g. stirrer), energy saving, easy<br />
retrofi tting in existing plants and saving of<br />
time.<br />
Direct steam injection is used to heat <strong>the</strong><br />
mash; <strong>the</strong> additional injection of inert gas is<br />
used to achieve an adequate homogenisation.<br />
The presentation will show results, which describe<br />
heat transfer rates and <strong>the</strong> distribution<br />
of temperature inside <strong>the</strong> plant. Mash quality<br />
parameters are compared to classical mash<br />
tuns. Additional <strong>the</strong> DMS evaporation during<br />
<strong>the</strong> mashing process under diff erent conditions<br />
are monitored.<br />
Based on <strong>the</strong>se results, a combined mash and<br />
lauter tun without mechanical devices is <strong>the</strong><br />
aim of this research.<br />
Matthias Kern<br />
Matthias Kern, born in 1984, graduated from<br />
Technische Universität München in 2011 as<br />
a degreed engineer <strong>for</strong> brewing sciences<br />
and beverage technology. The same year he<br />
started his doctoral <strong>the</strong>sis with Prof. Thomas<br />
Becker at <strong>the</strong> institute of brewing and beverage<br />
technology. His research project is <strong>the</strong><br />
‚Development of a new mash and lauter technology‘.<br />
L 47<br />
High mashing-off temperature and<br />
acidifi cation of sparging water extending<br />
fl avour stability of beer<br />
Gert De Rouck 1, Guido Aerts 1<br />
1KaHo St.-Lieven, Industrial Engineer, Gent, Belgium<br />
DESCRIPTION OF TOPIC:<br />
Boiling during brewing is unavoidable <strong>for</strong><br />
many reasons. However, it requires high<br />
energy input and results in decreased fi nal<br />
beer quality and stability. A new approach<br />
was proposed in which high mashing-off<br />
temperature was applied in combination with<br />
acidifi ed sparging water and in-line steam<br />
injections. This resulted in suffi cient <strong>for</strong>mation<br />
and stripping of DMS, early fl occulation<br />
of haze sensitive protein, limited extraction<br />
of polyphenols, and higher utilization yield<br />
of hop α-acids. Acidifi ed sparging resulted<br />
in higher levels of free aldehydes in pitching<br />
wort. Never<strong>the</strong>less, <strong>the</strong> amount of free aldehydes<br />
in beer was low. However, upon <strong>for</strong>ced<br />
ageing, <strong>the</strong> increase in aldehydes was lower<br />
compared with traditional brewing. The overall<br />
ageing score was very low. Presumably,<br />
aldehydes were released from a bound state<br />
during wort production by acidifi ed sparging.<br />
Subsequent reduction during fermentation<br />
leads to an extended beer fl avour stability,<br />
combined with cost effi cient brewing.<br />
Gert De Rouck<br />
Born in 1974. Studies: Academic degree in<br />
Industrial Engineering Biochemistry at KaHo<br />
St.- Lieven, Gent, Belgium (1996). Appointments:<br />
1996-2000: Product development at<br />
<strong>the</strong> proef brewery. 2000 present: Assistant<br />
Professor at KaHo SL. Master brewer of pilot<br />
brewery (2-5 hl); courses in malting and<br />
brewing technology; assistant scientist in<br />
Malting and Brewing Technology at EFBT,<br />
KaHo SL. Research topics: fl avour stability,<br />
mixed fermentations. PhD study (since 2008):<br />
cost effi cient brewing.<br />
L 48<br />
Infl uence of starch sources and<br />
mashing procedures on <strong>the</strong> range<br />
of Molecular Weight Distribution<br />
(MWD) and <strong>the</strong> intensity of palate<br />
fullness of beer<br />
Heinrich Rübsam 1, Martin Krottenthaler 2,<br />
Martina Gastl 1, Thomas Becker 1<br />
1Technische Universität München (TUM), Chair of Brewing<br />
and Beverage Technology, Freising, Germany, 2Hochschule<br />
Weihenstephan-Triesdorf, Freising, Germany<br />
DESCRIPTION OF TOPIC:<br />
The MWD (AF4/MALLS/RI) and intensity of<br />
palate fullness (Sensory analysis: DIN 10952)<br />
were compared among beers produced varying<br />
mashing procedures (infusion/decoction,<br />
initial temperature) and starch sources<br />
(maize, spelt, malted barley). T<strong>here</strong> were no<br />
diff erences on <strong>the</strong> MWD using infusion or decoction<br />
w<strong>here</strong>as signifi cant diff erences (p<<br />
0.05) were observed according to <strong>the</strong> initial<br />
temperature conditions. Slight diff erences on<br />
<strong>the</strong> MWD were appreciated using maize 30%<br />
(without enzymes addition) compared to <strong>the</strong><br />
observed using malted barley (41% Kolbach<br />
Index (KI)). The use of non malted spelt (30%)<br />
caused higher MWD (p< 0.005) than <strong>the</strong> use<br />
of maize (30%) or malted barley. Lower (p<<br />
0.005) intensities of palate fullness were appreciated<br />
in beers produced with maize 30%<br />
and malted barley (41% KI) compared to those<br />
with malted barley (36% KI) and non malted<br />
spelt (30%). T<strong>here</strong><strong>for</strong>e starch sources and <strong>the</strong><br />
gradient of mashing temperature infl uenced<br />
<strong>the</strong> MWD and <strong>the</strong> palate fullness of <strong>the</strong> beer.<br />
Heinrich Rübsam<br />
Heinrich Rübsam completed his Dipl.-Eng.<br />
degree in chemical engineering at <strong>the</strong> Metropolitan<br />
University, Caracas, Venezuela, in<br />
July 2004. Later, he studied brewing and beverage<br />
technology at <strong>the</strong> Technische Universität<br />
München in Germany and obtained his<br />
M.S. degree in 2009. Currently he is pursuing<br />
his Ph.D. at <strong>the</strong> Chair of Brewing and Beverage<br />
Technology, TUM-Weihenstephan. His<br />
research focus is on <strong>the</strong> Infl uence of starch<br />
degradation during mashing on <strong>the</strong> intensity<br />
of palate fullness of beer<br />
39 34 TH EBC CONGRESS · LUXEMBOURG 26 – 30 MAY 2013
L 49<br />
Changes in protein profi le and enzymatic<br />
activity during mashing when<br />
using diff erent grist compositions<br />
of barley and wheat malt and <strong>the</strong>ir<br />
infl uence to beer quality<br />
Andrea Faltermaier 1, Thomas Becker 2,<br />
Elke K Arendt 1, Martina Gastl 3<br />
1University College Cork, School of Food and Nutritional<br />
Sciences, Cork, Ireland, 2Technische Universität München,<br />
Center of Life Sciences Weihenstephan, Lehrstuhl für<br />
Brau und Getränketechnologie, Freising, Germany, 3TUM<br />
Weihenstephan, Lehrstuhl für Brau und Getränketechnologie,<br />
Freising, Germany<br />
DESCRIPTION OF TOPIC:<br />
A fundamental study on protein pofi le and<br />
enzymatic activity during mashing using different<br />
grist compositions of barley and wheat<br />
malt was carried out. Samples were analyzed<br />
of every single mashing step to get a deeper<br />
insight in breakdown processes which take<br />
place during mashing. Changes in content<br />
and molecular fractions were investigated<br />
during <strong>the</strong>se steps.<br />
In this study variations in protein profi le and<br />
enzyme activity depending to <strong>the</strong> grist ratio<br />
(barley:wheat) are shown. The mashes were<br />
analyzed according to MEBAK and EBC methods.<br />
Protein fractions were analysed using<br />
a Lab-on-a-Chip technique, and OFFGEL, thus,<br />
providing a deeper insight into <strong>the</strong> proteolysis<br />
associated with mashing. All results were<br />
compared to <strong>the</strong> changes taking place during<br />
<strong>the</strong> mashing of barley. With those results possible<br />
optimization of fi nal beer quality depending<br />
to <strong>the</strong> grist ratio and optimal mashing<br />
process of <strong>the</strong> used malts are shown.<br />
Andrea Faltermaier<br />
Andrea Faltermaier studied Food Technology<br />
at <strong>the</strong> Technische Universität München,<br />
Weihenstephan. She carried out her diploma<br />
<strong>the</strong>sis at <strong>the</strong> TUM-Weihenstephan. Since<br />
2009 Andrea Faltermaier is a PhD student at<br />
<strong>the</strong> University College Cork, and she got <strong>the</strong><br />
InBev-Baillet Latour Scholarship in Brewing<br />
and Malting. Her PhD project, a cooperation<br />
between UCC and TUM, deals with studies on<br />
<strong>the</strong> application of wheat in brewing and functional<br />
beverages.<br />
L 50<br />
Procedural considerations to <strong>the</strong><br />
starch conversion during mashing<br />
Simon Henke 1, Julian Cuperus 1, Karl<br />
Sommer 1<br />
1TU München, Chair of Process Engineering of Disperse<br />
Systems, Freising, Germany<br />
DESCRIPTION OF TOPIC:<br />
Water uptake, swelling and fi nally gelatinization<br />
of starch are evident <strong>for</strong> its enzymatic<br />
degradation. These mechanisms are accompanied<br />
with particulate changes. During<br />
mashing <strong>the</strong> granule size development overlaps<br />
with grist agglomerate disintegration<br />
and enzymatic reactions, especially at higher<br />
temperatures. For <strong>the</strong>se reasons an unequivocally<br />
assignment which process contributes<br />
in which way to <strong>the</strong> particle size change,<br />
leaves much room <strong>for</strong> misinterpretations.<br />
The processes of swelling, water uptake and<br />
enzymatic reactions were investigated separately<br />
to clear up with <strong>the</strong> mentioned problems.<br />
Particle sizes and its changes were<br />
quantifi ed via laser diff raction and microscope.<br />
Fur<strong>the</strong>rmore <strong>the</strong> speed of each reaction<br />
was determined. The results show how<br />
<strong>the</strong> enzymatic attack of α- and ß-amylase is<br />
aff ected by <strong>the</strong> reaction of starch with water<br />
and its temperature dependency. The achieved<br />
results deliver a deeper understanding of<br />
<strong>the</strong> mechanisms of starch degradation during<br />
mashing.<br />
Simon Henke<br />
Simon Henke graduated from Technical University<br />
Munich in 2009 as a degreed engineer<br />
<strong>for</strong> brewing sciences and beverage technology.<br />
In 2010 he started his work at <strong>the</strong> Chair<br />
of Process Engineering of Disperse Systems,<br />
Technische Universität München as a<br />
research associate. His fi elds of activity are<br />
mass transport phenomena and procedural<br />
aspects of <strong>the</strong> mashing process. Simon is involved<br />
in <strong>the</strong> students education in <strong>the</strong>rmal<br />
and mechanical process engineering. He is<br />
responsible <strong>for</strong> <strong>the</strong> pilot plant brewery at <strong>the</strong><br />
Chair of Process Engineering as well as <strong>for</strong><br />
<strong>the</strong> laboratory <strong>for</strong> wet and dry particle size<br />
analysis.<br />
40<br />
L 51<br />
Development of methods to measure<br />
real-time fl avour profi les<br />
Deborah Parker 1, Eleanor Woods 1<br />
1Campden BRI, Brewing Services, Nutfi eld, United Kingdom<br />
DESCRIPTION OF TOPIC:<br />
Brewers carefully select <strong>the</strong> best raw materials<br />
to provide <strong>the</strong> ideal fl avour profi le <strong>for</strong><br />
<strong>the</strong>ir beers. But how can <strong>the</strong> brewer be sure<br />
that <strong>the</strong> raw materials <strong>the</strong>y have selected will<br />
provide <strong>the</strong> intensity and length of fl avour that<br />
<strong>the</strong>y desire?<br />
It is already known that <strong>the</strong> release of fl avour<br />
volatiles during consumption can be measured<br />
analytically, but this can also be achieved<br />
with more meaningful eff ect by using a trained<br />
panel of tasters. This provides a model of <strong>the</strong><br />
‚real-time‘ drinking fl avour profi le that customers<br />
are experiencing. This study details <strong>the</strong><br />
employment of novel sensory methods, such<br />
as Temporal Dominance of Sensations (TDS)<br />
to measure <strong>the</strong> real-time perception of fl avour<br />
from a range of beer styles. In addition,<br />
<strong>the</strong> study will involve <strong>the</strong> measurement of key<br />
fl avour volatiles and non-volatile compounds<br />
in order to correlate sensory and analytical<br />
data and identify <strong>the</strong> compounds essential <strong>for</strong><br />
<strong>the</strong> deliverance of dominant and lasting fl avours<br />
in beer.<br />
Deborah Parker<br />
Deborah is a Sensory Scientist with an Honours<br />
Degree in Biochemistry, a Post Graduate<br />
Certifi cate in Sensory Science and a<br />
Doctorate in Brewing Science. She manages<br />
and trains <strong>the</strong> BRI Expert, Technical and QDA<br />
panels. Deborah is an accredited trainer, having<br />
lectured at <strong>the</strong> Siebels Institute in Chicago<br />
and EBC Conventions. She is a member of<br />
EBC Analysis Sensory Sub-group committee<br />
and <strong>the</strong> IBD Hop Industry Committee.
L 52<br />
Relationships between <strong>the</strong> chemical-physical<br />
composition, <strong>the</strong><br />
sensory descriptive analysis and<br />
consumer acceptance of German<br />
wheat beer<br />
Annette Schmelzle 1, Bernd Lindemann 1,<br />
Frank-Jürgen Methner 2<br />
1Hochschule Geisenheim University, Geisenheim, Germany,<br />
2Technische Universität Berlin, Chair of Brewing Sciences,<br />
Berlin, Germany<br />
DESCRIPTION OF TOPIC:<br />
In order to determine which components contribute<br />
in any quantity to a high level of user<br />
acceptance, sensory descriptive analysis and<br />
<strong>the</strong> consumer acceptance of 16 wheat beers<br />
is conducted in addition to instrumental analyses.<br />
By linking <strong>the</strong> profi le test and <strong>the</strong> acceptance<br />
values, signifi cant attributes that<br />
are responsible <strong>for</strong> acceptance can be detected.<br />
The content of phenols, esters and higher<br />
aliphatic alcohols are compared against <strong>the</strong><br />
sensory descriptive characteristics.<br />
The high acceptance of a banana-like odour<br />
and a creamy and tingling mouthfeel can be<br />
confi rmed. The preferred banana-like smelling<br />
beers show isophentyl acetate levels<br />
between 5.5 to 6.0 mg/l and thus exceed <strong>the</strong><br />
recommended value of < 4.5 mg/l. That <strong>the</strong><br />
odour of wheat beer with more than 3 mg/l<br />
2-methoxy-4-vinyl phenol is too phenolic,<br />
could not be confi rmed. Wheat beers that<br />
have a 2-methoxy-4-vinyl phenol content of ><br />
3 mg/l are also preferred if <strong>the</strong>y have additionally<br />
a fruity odour and a creamy mouthfeel.<br />
Annette Schmelzle<br />
Annette Schmelzle is an experienced engineer<br />
of beverage technology in sensory evaluation<br />
of beer, cola, wine and mineral water.<br />
In 2009 she completed her studies at <strong>the</strong><br />
Hochschule Geisenheim University in Germany.<br />
Currently she works at <strong>the</strong> Hochschule<br />
Geisenheim University as research fellow<br />
and prepares her dissertation in ‚Sensory descriptive<br />
analysis and consumer acceptance<br />
of diff erent beer types‘ at <strong>the</strong> TU Berlin<br />
L 53<br />
Comparison of two techniques <strong>for</strong><br />
maturation of beer using oak<br />
Luis Henrique Poleto Angeloni 1, Patricia<br />
Wyler 1, Aline Bortoletto 1<br />
1University of São Paulo, Agro-Food Industry and Nutrition,<br />
Piracicaba, Brazil<br />
DESCRIPTION OF TOPIC:<br />
Aims: Identify <strong>the</strong> best technique to extract<br />
aromatics compounds into beers matured in<br />
oak barrel or by oak cubes and verify <strong>the</strong> infl<br />
uence of toasting on <strong>the</strong> extraction by cubes.<br />
Methods: We quantifi ed aromatics compounds<br />
(gallic, vanilic, and syringic acids;<br />
siringaldehyde; coniferaldehyde; sinapaldehyde;<br />
vanillin; furfural and 5-hydroxymethylfurfural)<br />
by High Per<strong>for</strong>mance Liquid Chromatography<br />
in lager beer matured <strong>for</strong> three<br />
months at 0 °C in barrel or by cubes with<br />
three diff erent levels of toasting(light, medium<br />
or high).<br />
Results: After one and two months <strong>the</strong> beer<br />
matured with medium and high toasting cubes<br />
extracted <strong>the</strong> greatest amount of <strong>the</strong><br />
compounds. After three months <strong>the</strong> beer<br />
matured by high toasting cubes presented<br />
<strong>the</strong> highest concentration of total maturation<br />
compounds(28 mg/L), followed by <strong>the</strong> beer<br />
matured in barrel(23 mg/L).<br />
Conclusions: The use of oak cubes is an<br />
effi cient technique to extract aromatic compounds<br />
into beer and toasting has positive<br />
infl uence on maturation.<br />
Luis Henrique Poleto Angeloni<br />
Biologist with Masters degree in Biotechnology<br />
alcoholic fermentation, currently a doctoral<br />
student in food science and technology.<br />
He worked as a brewer at Leuven brewing -<br />
Brazil. Acting on <strong>the</strong> following <strong>the</strong>mes: yeast,<br />
alcoholic fermentation, toxicity, fermentation<br />
of beverages, biotechnology, applied microbiology<br />
and biotechnological processes <strong>for</strong><br />
beer production.<br />
L 54<br />
Biogeography, population genomics<br />
and technological properties of <strong>the</strong><br />
lager-brewing ancestor Saccharomyces<br />
eubayanus<br />
Diego Libkind 1, Martin Bontrager 2, Jose<br />
Paulo Sampaio 3, Chris T Hittinger 2<br />
1INIBIOMA (CONICET-UNComahue), Bariloche, Argentina,<br />
2University of Wisconsin, Madison, United States,<br />
3Universidade Nova de Lisboa, Caparica, Portugal<br />
DESCRIPTION OF TOPIC:<br />
It has been long known that <strong>the</strong> lager-brewing<br />
yeast Saccharomyces pastorianus resulted<br />
from <strong>the</strong> fusion of S. cerevisiae and a coldadapted<br />
species belonging to <strong>the</strong> S. bayanus/<br />
uvarum species complex. We discovered in<br />
Patagonia <strong>the</strong> missing contributor of lager<br />
yeast: S. eubayanus. To understand its biogeographic<br />
distribution and population genetics,<br />
we collected over 200 additional strains<br />
in <strong>the</strong> argentinean Andes along a 1,500 km<br />
longitudinal transect. Genomic analysis<br />
showed that S. eubayanus in Patagonia is<br />
more diverse than S. cerevisiae and that highly<br />
divergent strains can be found within small<br />
geographic areas. In contrast, <strong>the</strong> analysis of<br />
genome sequences of several Lager strains<br />
confi rmed <strong>the</strong> low genetic divergence of <strong>the</strong><br />
criotolerant genome portion of this group.<br />
Preliminary data on <strong>the</strong> brewing experience<br />
with S. eubayanus will be addressed, as well<br />
as <strong>the</strong> available in<strong>for</strong>mation relevant to <strong>the</strong><br />
discussion on how and when <strong>the</strong> Lager hybrid<br />
might have been originated.<br />
Diego Libkind<br />
Licenciate in Biological Sciences, 2001,<br />
UNComahue. PhD in Biochemistry, 2006,<br />
UNTucuman. CONICET Researcher of <strong>the</strong><br />
Biodiversity and Environmental Research Institute<br />
(INIBIOMA), Bariloche, Argentina. Member<br />
of:International Commision on Yeasts, and<br />
ASBC Craft Brewing Committee.<br />
41 34 TH EBC CONGRESS · LUXEMBOURG 26 – 30 MAY 2013
L 55<br />
A new maltose/maltotriose transporter<br />
[Saccharomyces eubayanustype<br />
Agt1] in lager yeast and its<br />
relevance to fermentation per<strong>for</strong>mance<br />
Virve Vidgren 1, Jari Rautio 2, Laura Mattinen 2,<br />
Brian Gibson 1, John Londesborough 1<br />
1VTT Technical Research Centre of Finland, Espoo, Finland,<br />
2Plexpress Oy, Helsinki, Finland<br />
DESCRIPTION OF TOPIC:<br />
Fermentation per<strong>for</strong>mance of lager yeast is<br />
determined by uptake of fermentable sugar<br />
in a low temperature environment. A new putative<br />
maltose/maltotriose transporter gene<br />
was identifi ed from lager strain WS34/70 and<br />
suggested to be <strong>the</strong> Saccharomyces eubayanus<br />
(Se) version of <strong>the</strong> Agt1 transmembrane<br />
transporter in <strong>the</strong> hybrid lager yeast S. pastorianus<br />
(S. cerevisiae x S. eubayanus). In <strong>the</strong><br />
present work, <strong>the</strong> transporter coded by this<br />
putative AGT1 gene was characterized and<br />
its relative activity at low temperature assessed.<br />
Results showed that SeAGT1 encodes<br />
a functional α-glucoside transporter with a<br />
wide substrate range, including maltose and<br />
maltotriose. The Se <strong>for</strong>m of <strong>the</strong> transporter<br />
derived from <strong>the</strong> cryotolerant S. eubayanus<br />
species was found to be less sensitive to low<br />
temperature conditions than <strong>the</strong> S. cerevisiae<br />
<strong>for</strong>m. Thus, SeAGT1 may have contributed to<br />
<strong>the</strong> superior per<strong>for</strong>mance of lager yeasts in<br />
low temperature fermentations compared to<br />
ale yeasts.<br />
Virve Vidgren<br />
Virve Vidgren (PhD) is research scientist<br />
working in <strong>the</strong> fi eld of Yeast Physiology and<br />
Fermentations within VTT Bio- and chemical<br />
processes. She has broad experience in yeast<br />
genetics and physiology. One focus of her studies<br />
has been transporters involved in uptake<br />
of sugars during wort fermentations.<br />
L 56<br />
Development of <strong>the</strong> direct DNA extraction<br />
method <strong>for</strong> rapid detection<br />
of low concentrations of beer-spoilage<br />
lactic acid bacteria<br />
Masaki Shimokawa 1, Satomi Naito 1,<br />
Kazumaru Iijima 2, Yasuo Motoyama 3,<br />
Tomomichi oga 1, Koichi Harayama 1<br />
1Asahi Breweries Ltd., Quality Control Center, Moriya, Japan,<br />
2Asahi Breweries Ltd., Research and Development Laboratories<br />
<strong>for</strong> Brewing, Moriya, Japan, 3Asahi Breweries Ltd.,<br />
Nagoya <strong>Brewery</strong>, Nagoya, Japan<br />
DESCRIPTION OF TOPIC:<br />
We aimed to develop a rapid and highly sensitive<br />
method <strong>for</strong> <strong>the</strong> detection of beer-spoilage<br />
lactic acid bacteria (LAB) in beer, based on<br />
polymerase chain reaction combined with <strong>the</strong><br />
direct DNA extraction method from <strong>the</strong> trapped<br />
cells within <strong>the</strong> cellulose membrane by<br />
using Barocycler. Barocycler is <strong>the</strong> device<br />
making cycles of extreme high and ordinary<br />
pressure conditions in a reaction vessel. These<br />
pressure cycles are expected to enhance<br />
<strong>the</strong> penetration of DNA extracting solution<br />
into <strong>the</strong> matrix of membrane and disrupt<br />
cells eff ectively. The detection limits of our<br />
developed method was about 10^0-1 cells/<br />
membrane, when Lactobacillus brevis was<br />
suspended in from 300 ml up to 3000 ml of<br />
beers. These results indicate that our direct<br />
DNA extraction approach makes it possible to<br />
apply larger sample volume than previous reports<br />
without compromising recovery of DNA.<br />
Taken toge<strong>the</strong>r, our method was indicated to<br />
be applicable to beers contaminated with low<br />
concentrations of beer-spoilage LAB.<br />
Masaki Shimokawa<br />
Received a ME degree in Environmental Engineering<br />
from Hokkaido University, Japan,<br />
in 2008, w<strong>here</strong> he majored in environmental<br />
microbiology. He joined Asahi Breweries,<br />
Ltd. in April 2008. Since September 2011, he<br />
has been working on microbiological quality<br />
assurance in breweries and developing <strong>the</strong><br />
detection technology of beer spoilage microorganisms<br />
in Quality Control Center.<br />
42<br />
L 57<br />
Evaluation of biofi lm composition<br />
and maturation using realtime-PCR<br />
Mathias Hutzler 1, Robert Riedl 1, Jennifer<br />
Koob 1, Fritz Jacob 1<br />
1TUM Weihenstephan, Research Center Weihenstephan,<br />
Freising, Germany<br />
DESCRIPTION OF TOPIC:<br />
Biofi lms are a major source of microbial contamination<br />
and hygienic risks within <strong>the</strong> brewing<br />
environment. Biofi lms consist of diff erent<br />
microbial fractions. Amongst o<strong>the</strong>r fractions<br />
<strong>the</strong>y can contain biofi lm starters, slime/EPS<br />
<strong>for</strong>ming bacteria, gram-positive beer spoiling<br />
bacteria, strictly anaerobic gram-negative<br />
beer spoiling bacteria and diff erent species of<br />
yeast. In this study a modular PCR-Screening<br />
assay was designed and evaluated to detect a<br />
wide spectrum of bacteria and yeasts, which<br />
are involved in <strong>the</strong> growth of biofi lms. The<br />
quantitative evaluation of <strong>the</strong> diff erent microbial<br />
fractions of <strong>the</strong> biofi lm enabled a classifi<br />
cation of biofi lms into diff erent levels of maturation<br />
and contamination risk. The identities<br />
were linked with data about <strong>the</strong> organisms,<br />
containing metabolic products, product risk<br />
and typical locations. Additionally typical indicator<br />
organisms <strong>for</strong> <strong>the</strong> biofi lm maturation<br />
level and <strong>for</strong> subsequent product risk were<br />
identifi ed.<br />
Mathias Hutzler<br />
Born in 1978 in Regensburg, Germany.<br />
1999-2004 studies and graduation in Food<br />
Technology and Biotechnology, TU München.<br />
2004-2009 Scientifi c Assistant at <strong>the</strong> Chair<br />
<strong>for</strong> Brewing Technology II, TU München. Topic<br />
of doctoral <strong>the</strong>sis: Diff erentiation of industrial<br />
and spoilage yeasts based on novel rapid<br />
methods. Since 2009 head of <strong>the</strong> yeast center<br />
and <strong>the</strong> accredited laboratory <strong>for</strong> brewing/<br />
beverage microbiology at <strong>the</strong> Research Center<br />
Weihenstephan <strong>for</strong> Beer and Food Quality,<br />
TU München.
L 58<br />
Diversity of xylanolytic bacteria and<br />
xylanase genes during industrial<br />
malting<br />
Sofi e Malfl iet 1, Annelies Justé 2, Bart<br />
Lievens 2, Luc De Cooman 1, Guido Aerts 1<br />
1KAHO St.-Lieven, Laboratory of Enzyme, Fermentation, and<br />
Brewing Technology (EFBT), Department Chemistry/Biochemistry<br />
- LFoRCe, Department M2S, KU Leuven, Gent, Belgium,<br />
2Lessius Hogeschool, Laboratory <strong>for</strong> Process Microbial Ecology<br />
and Bioinspirational Management (MPE&BIM) - LFoRCe,<br />
Department M2S, KU Leuven, Sint-Katelijne-Waver, Belgium<br />
DESCRIPTION OF TOPIC:<br />
The presence of microorganisms producing<br />
cell wall hydrolysing enzymes such as xylanases<br />
during malting can improve mash fi ltration<br />
behaviour and consequently have potential<br />
<strong>for</strong> more effi cient wort production and<br />
enhanced beer fl avour quality and stability.<br />
T<strong>here</strong><strong>for</strong>e, arabinoxylan-degrading bacteria<br />
occurring on <strong>the</strong> grains during malting were<br />
isolated, screened <strong>for</strong> xylanase activity, and<br />
identifi ed by 16S rRNA gene sequencing. In<br />
this way, xylanolytic bacteria such as Sphingobacterium<br />
multivorum, Stenotrophomonas<br />
maltophilia and Aeromonas salmonicida were<br />
identifi ed. In addition, as especially microbial<br />
xylanases belonging to <strong>the</strong> glycosyl hydrolase<br />
(GH) family 10 are highly interesting <strong>for</strong> malting<br />
process optimisation, we also assessed<br />
<strong>the</strong> diversity and distribution of <strong>the</strong>se xylanase<br />
genes by 454 pyrosequencing during malting,<br />
i.e. from barley up to kilned malt. Results<br />
provide a deep insight into <strong>the</strong> occurrence<br />
and diversity of xylanase sequences of microbial<br />
origin during malting.<br />
Sofi e Malfl iet<br />
In 2004, Sofi e Malfl iet obtained her MSc in<br />
Industrial Sciences in Biochemistry at KAHO<br />
St.-Lieven in Ghent, Belgium. After graduation,<br />
she started working as a Research Assistant<br />
at <strong>the</strong> Laboratory of Enzyme, Fermentation<br />
and Brewing Technology of KAHO St.-Lieven<br />
on fl avour quality and fl avour stability of pilsner<br />
and light beers. She is currently fi nishing<br />
her PhD on microfl ora management during<br />
malting, in particular on xylanase-producing<br />
bacteria and <strong>the</strong>ir infl uence on wort fi ltration<br />
rate.<br />
L 59<br />
Microbiological analysis of a shipwreck<br />
beer<br />
Riikka Juvonen 1, Erna Storgårds 1, Mari<br />
Raulio 1, Thomas Hofmann 2, Atte Mikkelson 1,<br />
Annika Wilhelmson 1, Michael Dresel 2, Brian<br />
Gibson 1, John Londesborough 1<br />
1VTT Technical Research Centre of Finland, Bio- and Process<br />
Technology, Espoo, Finland, 2Technische Universtität<br />
München, Chair of Food Chemistry and Molecular Sensory<br />
Science, Freising, Germany<br />
DESCRIPTION OF TOPIC:<br />
In 2010 <strong>the</strong> wreck of a schooner was discovered<br />
in <strong>the</strong> Baltic Sea. The schooner is<br />
believed to have sunk during <strong>the</strong> 1840´s.<br />
Five bottles of beer were raised from it. We<br />
attempted to isolate and characterize living<br />
microbes from two intact bottles.Many diff erent<br />
microbes were observed by electron microscopy,<br />
including dead yeast cells. DNA was<br />
amplifi ed using primers targeting phylogenetic<br />
markers. Yeast DNA was detected, but no<br />
viable yeast cells were recovered. Remarkably<br />
stable non-sporulating lactic acid bacteria<br />
were found alive in both beers, following cultivation<br />
in various media. The isolates were<br />
characterised. Four species of Lactobacillus<br />
and Pediococcus and many genotypes were<br />
identifi ed. Their physiological properties indicate<br />
good adaptation to brewing conditions.<br />
The ability <strong>for</strong> long survival is not linked to<br />
a certain species or genus. The live bacteria<br />
are interesting models <strong>for</strong> study of long-term<br />
survival mechanisms, and may provide insight<br />
with practical applications.<br />
Riikka Juvonen<br />
Riikka Juvonen, DSc in microbiology, has<br />
more than 10 years of experience in characterization,<br />
detection and management of microbial<br />
communities in industrial processes,<br />
especially in <strong>the</strong> fi eld of brewing and baking.<br />
Her recent research activities have focused<br />
on bioprocessing of plant-based materials <strong>for</strong><br />
<strong>the</strong>ir technological and nutritional upgrading,<br />
and on management of microbial risks in<br />
novel types of non-beer beverages. Riikka<br />
is currently working at VTT Bio and Process<br />
Technology as a Senior Scientist.<br />
L 60<br />
Total process optimization via<br />
KAIZEN-ASAHI WAY: Technological<br />
innovations spreading system.<br />
Yoshinori Ito 1, Tomohisa Achioku 2<br />
1Asahi Breweries Ltd., Production Technology Center, Ibaragi,<br />
Japan, 2Asahi Breweries Ltd., Production Strategy Department,<br />
Tokyo, Japan<br />
DESCRIPTION OF TOPIC:<br />
We reported “Constructing an Improvement<br />
Initiative via a “Visualized” System” at 33rd<br />
<strong>European</strong> <strong>Brewery</strong> Convention Congress in<br />
2010. We have been refi ning “KAIZEN-ASAHI<br />
WAY” through <strong>the</strong> common improvement issue.<br />
We minimized <strong>the</strong> variation in quality of<br />
beer fl avor and improved <strong>the</strong> tasting score of<br />
beer. In addition we have achieved a reduction<br />
of production cost by more than €10,000,000<br />
in three years. At fi rst professional development<br />
team is working on technology innovation<br />
with top runner brewery, and <strong>the</strong>se innovative<br />
technologies are packaged in standard<br />
equipment and standard operation. Then we<br />
could easily expand <strong>the</strong>se fruits to o<strong>the</strong>r breweries.<br />
The Asahi- improvement-initiatives<br />
model will be presented through some actual<br />
cases.<br />
Yoshinori Ito<br />
In 1989 I joined Asahi Breweries. After joining<br />
<strong>the</strong> company, I was in charge of a new product<br />
evelopment and put some new product<br />
in market. Nagoya <strong>Brewery</strong>, Brewing section<br />
in 1996. Development Laboratories <strong>for</strong> Alcoholic<br />
Beverages, Department of Beer Product<br />
Development, Manager in 1999. Marketing<br />
Headquarters in 2000. Deputy General Manager<br />
of Ibaragi <strong>Brewery</strong> in 2006. Produciton<br />
Technology Center, General Manager in 2009.<br />
43 34 TH EBC CONGRESS · LUXEMBOURG 26 – 30 MAY 2013
L 61<br />
Bitter substance yield during <strong>the</strong><br />
brewing process - Infl uencing factors<br />
and possibilities of recovery<br />
Frank-Jürgen Methner 1, Thomas Kunz 1,<br />
Philip C. Wietstock 1, Mario Marinoff 1, Matthias<br />
Gaulke 1<br />
1Technische Universität Berlin, Lab of Brewing Science,<br />
Berlin, Germany<br />
DESCRIPTION OF TOPIC:<br />
Bitter substance utilization during conventional<br />
beer production is considerably low and<br />
still a major problem <strong>for</strong> <strong>the</strong> brewing industry.<br />
Brewing trials were conducted to evaluate <strong>the</strong><br />
eff ect of pH and Fe on bitter substance yield.<br />
In addition to fermentation trials, hopped and<br />
unhopped wort w/ and w/o Fe addition was<br />
adjusted to diff erent pH values. The bitter<br />
substance yield was signifi cantly infl uenced<br />
by both, pH and Fe concentration. Acidifying<br />
<strong>the</strong> system yielded reduced bitter units, hop<br />
acid and Fe concentrations. Regardless of<br />
initial pH, bringing <strong>the</strong> pH to alkaline conditions<br />
again produced <strong>the</strong> highest results. The<br />
Fe content in unhopped wort was unaff ected<br />
by <strong>the</strong> pH adjustments. Taken toge<strong>the</strong>r, <strong>the</strong>se<br />
results suggest that reversible, pH dependent<br />
hop acid-Fe-complexes are <strong>for</strong>med and<br />
mainly responsible <strong>for</strong> <strong>the</strong> loss of BU during<br />
fermentation.<br />
Additional trials are presented which demonstrate<br />
that outcomes from this research<br />
can be used to e.g. recover hop acids from hot<br />
trub.<br />
Frank-Jürgen Methner<br />
From 1975 to 1981 studying Brewing Science<br />
at TU Berlin. fi nishing with a degree Diplom-<br />
Ingenieur (Dipl-Ing.) From 1982 to 1986 Scientifi<br />
c Assistant at Research Institute <strong>for</strong><br />
Brewing and Malting Technology of <strong>the</strong> VLB<br />
in Berlin. From 1987 till 2004 Director at <strong>the</strong><br />
Bitburger Brauerei, Bitburg, Germany, with<br />
responsibilities in fi elds of technology and<br />
quality assurance. Since winter-semester<br />
2004/2005 taking over <strong>the</strong> chair of Brewing<br />
Science at TU Berlin.<br />
POSTER ABSTRACTS<br />
P 001<br />
Soluble dietary fi ber as food ingredients<br />
from brewers‘ spent yeast<br />
polysaccharides<br />
Tiago Brandão 2, Elisabete Coelho 1,<br />
Mariana Pinto 1, Tiago Brandão 2,<br />
Manuel A. Coimbra 1<br />
1Universidade de Aveiro, Department of Chemistry, Aveiro,<br />
Portugal,<br />
2Unicer Bebidas, SA, Porto, Portugal<br />
DESCRIPTION OF TOPIC:<br />
Brewers‘ spent yeast (BSY) is <strong>the</strong> second<br />
major by-product of brewery industry. These<br />
residues comprise polysaccharides, namelyglucans<br />
and mannoproteins, which should be<br />
recovered due to <strong>the</strong>ir bioactive properties.<br />
In order to solubilize all <strong>the</strong>se polysaccharides,<br />
a sequential extraction with hot water<br />
and alkali solutions from 0.1M to 8M of KOH,<br />
followed by a partial acid hydrolysis was per<strong>for</strong>med.<br />
The 4M KOH solubilized 6% of <strong>the</strong> carbohydrates,<br />
corresponding to 26% of <strong>the</strong> total mannoproteins<br />
and 1% of glucans. The 8M KOH<br />
solubilized 18% of <strong>the</strong> carbohydrates, corresponding<br />
to 1% of total mannoproteins and<br />
22% of glucans. The glucans present in <strong>the</strong><br />
residue left were solubilized by partial acid<br />
hydrolysis. Using this procedure, BSY can be<br />
a source of cold water soluble polysaccharides<br />
able to be used as food ingredients to be<br />
incorporated in aqueous matrices as dietary<br />
fi ber.<br />
We thank FCT <strong>for</strong> fi nancial support of Research<br />
Unit 62/94-QOPNA and post doctoral<br />
grant SFRH/BPD/70589/2010.<br />
Tiago Brandão<br />
Tiago Brandao: Education: MSc Biotechnology<br />
– Leicester University, Biochemistry – Porto<br />
University, Brewmaster – Scandinavian<br />
School of Brewing. Current Positions: Innovation,<br />
Research & NPD Manager at Unicer Bebidas,<br />
SA since 2007, Executive Board member<br />
at Maltibérica, SA since 2007<br />
44<br />
P 002<br />
Si 3N 4-microsieves <strong>for</strong> a rapid<br />
detection of microbiological trace<br />
contaminations in beverages<br />
Roland Novy 1, Johannes Tippmann 1,<br />
Thomas Becker 1<br />
1Technische Universität München, Lehrstuhl für Brau- und<br />
Getränketechnologie, Freising, Germany<br />
DESCRIPTION OF TOPIC:<br />
In beverage industries, <strong>the</strong> state of <strong>the</strong> art <strong>for</strong><br />
<strong>the</strong> control on trace contaminations is still <strong>the</strong><br />
CFU (colony <strong>for</strong>ming unit), a combination of<br />
membrane fi ltration followed by an incubation<br />
on nutrient media. This method needs up<br />
to 7 days of incubation and contains possibilities<br />
of analysis errors and misinterpretation.<br />
The scope of this project is <strong>the</strong> development<br />
of a rapid detection method without incubation<br />
<strong>for</strong> microbiological analysis, based on<br />
cross fl ow microfi ltration with silicon nitride<br />
microsieves. The advantages of <strong>the</strong>se microsieves<br />
are low fi lter resistance, chemical<br />
inertness, <strong>the</strong>rmal stability, and <strong>the</strong>ir defi ned<br />
and narrow pore size distribution. The integration<br />
of <strong>the</strong>se microsieves into an automated<br />
microfi ltration system and <strong>the</strong> evaluation of<br />
<strong>the</strong> following microbiological analysis are <strong>the</strong><br />
main parts of this research. Due to <strong>the</strong> pure<br />
separation on <strong>the</strong> membrane surface, rapid<br />
analyses with optical fl uorescent techniques<br />
immediately after fi ltration are possible.<br />
Roland Novy<br />
Roland Novy graduated from Technische Univeristät<br />
Müchnen in 2010 as a degreed engineer<br />
<strong>for</strong> brewing sciences and beverage technology.<br />
The same year he started his doctoral<br />
<strong>the</strong>sis with Prof. Thomas Becker at <strong>the</strong> institute<br />
of brewing science and beverage technology.<br />
His research project is <strong>the</strong> ‚Deveploment<br />
of a rapid microbiological detection method<br />
based on silicon nitride microsieves‘
P 003<br />
A real-time next generation diagnostic<br />
tool <strong>for</strong> <strong>the</strong> malting and<br />
brewing industry: Selected ion fl ow<br />
tube mass spectrometry<br />
Jessika De Clippeleer 1, Filip Van Opstaele 1,<br />
Luc De Cooman 1, Guido Aerts 1<br />
1KAHO St.-Lieven, Laboratory of Enzyme, Fermentation, and<br />
Brewing Technology (EFBT), Department Chemistry/Biochemistry<br />
- LFoRCe, Department M2S, KU Leuven, Gent, Belgium<br />
DESCRIPTION OF TOPIC:<br />
Analytical measurement of beer fl avour related<br />
volatiles requires specifi c extraction and<br />
detection techniques to obtain <strong>the</strong> necessary<br />
sensitivity and selectivity. Current techniques<br />
hamper fast evaluation of intermediate samples<br />
during <strong>the</strong> brewing process, which is of<br />
paramount importance <strong>for</strong> process monitoring<br />
and improved control. Our data demonstrate<br />
that quantitative real-time measurement<br />
of target compounds by Selected Ion Flow<br />
Tube-Mass Spectrometry (SIFT-MS) is of high<br />
added value <strong>for</strong> ongoing malting and brewing<br />
research, and <strong>for</strong> subsequent applications<br />
in <strong>the</strong> brewing industry, e.g. quality control<br />
of raw materials (malt, hops), monitoring<br />
fermentation, fast evaluation of wort, fresh<br />
and aged beer quality parameters. W<strong>here</strong>as<br />
GC-MS is <strong>the</strong> technique of choice <strong>for</strong> detailed<br />
analytical volatile fi ngerprinting, it is not suited<br />
<strong>for</strong> real-time quantitative monitoring. In<br />
this respect, <strong>the</strong> SIFT-MS technology is fully<br />
complementary to GC-MS <strong>for</strong> quality control<br />
and process optimisation.<br />
Jessika De Clippeleer<br />
Jessika De Clippeleer is MSc in Engineering<br />
Technology, Biochemistry (KAHO Sint-Lieven,<br />
Gent) and MSc in Food Chemistry (Wageningen<br />
University). In 2013 she obtained <strong>the</strong> degree<br />
of Doctor in Bioscience Engineering (KU<br />
Leuven). She is Biochemistry lecturer, and comanager<br />
of Flavour+ (analytical centre of <strong>the</strong><br />
Enzyme, Fermentation, and Brewing Technology<br />
lab of KAHO Sint-Lieven), with research<br />
experience in fl avour (bio)chemistry, beer fl avour<br />
stability, hopping technology, and mass<br />
spectrometry.<br />
P 004<br />
Potential of near-infrared spectroscopy<br />
(NIR) in beer food chain:<br />
Applications in malting<br />
Paolo Fantozzi 1,<br />
Giuseppe Perretti 1, Valeria Sileoni 1,<br />
Ombretta Marconi 1,<br />
1University of Perugia, Italian Brewing Research Centre<br />
(CERB), Perugia, Italy<br />
DESCRIPTION OF TOPIC:<br />
Aims: This research aims to novel applications<br />
of NIR in beer food chain. Beyond <strong>the</strong><br />
mature utilizations of NIR <strong>for</strong> barley and malt<br />
quality assessment, <strong>the</strong> purpose was <strong>the</strong> development<br />
of new methods to monitor <strong>the</strong><br />
malting process and to predict <strong>the</strong> suitability<br />
of barley <strong>for</strong> beer production be<strong>for</strong>e malting.<br />
Methods: Barley were malted using micromalting<br />
pilot plant and <strong>the</strong>n analysed by<br />
standard A-EBC methods. The NIR absorption<br />
spectra were collected on barley, green malt<br />
and fi nal malt and correlated with <strong>the</strong> analytical<br />
data.<br />
Results: Fast, reliable and easy NIR calibration<br />
methods were developed to measure:<br />
• barley and malt quality parameters<br />
• malt quality parameters directly on barley<br />
as potential <strong>for</strong> malt features<br />
• malt parameters on green malt at-line during<br />
malting process.<br />
Conclusions: The use of NIR to monitor malting<br />
process and to predict malt parameters<br />
directly on barley can be extremely useful <strong>for</strong><br />
maltsters to sort it and handle it during malting,<br />
saving money and time.<br />
Paolo Fantozzi<br />
Born in Rome (Italy) in 1945. Master-degree<br />
in Agricultural Science (1967) and Doctorate<br />
in Biologie Appliquée Appliquée à la Nutrition<br />
et l‘Alimentation (Dijon, 1971). Since 1980<br />
Full Professor of Food Technology, Faculty of<br />
Agriculture. Since 2002 Director of <strong>the</strong> Italian<br />
Brewing Research Centre (CERB). His main<br />
research activities deal with brewing science<br />
and technology, mild technologies, quality<br />
control and management systems. Author<br />
of 230 papers on national and international<br />
Journals.<br />
P 005<br />
Quantitative cleaning investigations<br />
using falling fi lm<br />
Enrico Fuchs 1, Manuel Helbig 1, André Boye 2,<br />
Marc Mauermann 2, Jens-Peter Majschak 1,2<br />
1Technische Universität Dresden, Faculty of Mechanical<br />
Engineering, Institute of Processing Machines and Mobile<br />
Machines, Dresden, Germany, 2The Fraunhofer Application<br />
Center <strong>for</strong> Processing Machinery and Packaging Technology,<br />
Dresden, Germany<br />
DESCRIPTION OF TOPIC:<br />
Cleaning of machinery is crucial due to <strong>the</strong> required<br />
product safety and increasing production<br />
costs. Often a large quantity of cleaning<br />
fl uid is used to remove <strong>the</strong> deposits. This<br />
results in high costs <strong>for</strong> fresh- and wastewater.<br />
In non-immerged systems (e.g. vessels)<br />
t<strong>here</strong> are diff erent fl ow types providing <strong>the</strong><br />
cleaning eff ect (impinging jet, fi lm). Spray<br />
balls are often used <strong>for</strong> cleaning, w<strong>here</strong> <strong>the</strong><br />
main cleaning is provided by falling fi lm. An<br />
approach to reduce cleaning time and fl uid<br />
consumption is to optimize <strong>the</strong> cleaning by<br />
draining fi lm. To determine <strong>the</strong> cleaning rate,<br />
a quantitative optical method is used.<br />
In this paper<br />
(i) a reproducible soiling method and <strong>the</strong><br />
(ii) minimization of optical errors is shown.<br />
Also, <strong>the</strong><br />
(iii) calculation of <strong>the</strong> local distribution of<br />
soiling weights on samples is explained.<br />
Fur<strong>the</strong>rmore, <strong>the</strong><br />
(iv) validation of <strong>the</strong> method by cleaning with<br />
falling fi lms and its application is shown.<br />
The cleaning curves can be used to optimize<br />
cleaning of falling fi lms.<br />
Enrico Fuchs<br />
10/2004 12/2009: Diploma in Mechanical<br />
Engineering, Technische Universität Dresden<br />
since 06/2010: scientifi c assistant at Technische<br />
Universität Dresden<br />
45 34 TH EBC CONGRESS · LUXEMBOURG 26 – 30 MAY 2013
P 006<br />
Predicting <strong>the</strong> fl avor stability of<br />
beer using redox potential<br />
Dario Cotterchio 1<br />
1Technische Universität München (TUM), Forschungszentrum<br />
Weihenstephan für Brau- und Lebensmittelqualität (BLQ),<br />
Freising, Germany<br />
DESCRIPTION OF TOPIC:<br />
Changes in <strong>the</strong> freshness of a beer‘s fl avor<br />
are contingent on <strong>the</strong> free reaction enthalpy<br />
of all active redox pairs. In order to determine<br />
<strong>the</strong> enthalpy, an inert electrode is placed in a<br />
redox solution w<strong>here</strong> it <strong>the</strong>n accumulates a<br />
charge over a set period of time after which<br />
<strong>the</strong> corresponding voltage is measured. This<br />
reveals <strong>the</strong> scope of all <strong>the</strong> chemical processes<br />
occurring in <strong>the</strong> beer that involve <strong>the</strong><br />
exchange of electrons and serves as an indicator<br />
<strong>for</strong> <strong>the</strong> extent of <strong>the</strong> interplay between<br />
compounds, responsible <strong>for</strong> <strong>the</strong> development<br />
of perceptible sensory characteristics associated<br />
with aging. Experiments have shown<br />
that it is not <strong>the</strong> level of <strong>the</strong> redox potential<br />
but ra<strong>the</strong>r <strong>the</strong> change in <strong>the</strong> value while <strong>the</strong><br />
sample ages that correlates with <strong>the</strong> <strong>for</strong>mation<br />
of a perceptible level of aging compounds<br />
in beer. Various beer styles were tested and<br />
a distinctive electrochemical signature was<br />
established <strong>for</strong> each one, which allows <strong>for</strong> a<br />
reliable prediction of <strong>the</strong> fl avor stability to be<br />
made.<br />
Dario Cotterchio<br />
Dario Cotterchio was born in 1982 in Eschsur-Alzette,<br />
Luxembourg. He studied brewing<br />
and beverage technology at <strong>the</strong> Technische<br />
Universität München and graduated with <strong>the</strong><br />
degree Dipl.-Ing. in 2008. After graduation he<br />
worked in <strong>the</strong> engineering and biogas industry.<br />
Since April 2009 he is scientifi c assistant<br />
and a consulting engineer at <strong>the</strong> Research<br />
Center Weihenstephan <strong>for</strong> Brewing and Food<br />
Technology. He is working on <strong>the</strong> fi eld of development<br />
of <strong>the</strong> measure redox potential (ORP)<br />
in breweries.<br />
P 007<br />
Classifi cation of malting barley according<br />
to harvest year and quality<br />
by using mid infrared spectroscopy<br />
and multivariate analysis<br />
Budour Ajib 1, Frantz Fournier 1, Patrick<br />
Poivin 2, Marc Schmitt 2, Michel Fick 1<br />
1Lorraine University, Vandoeuvre les Nancy, France, 2IFBM,<br />
Vandoeuvre les Nancy, France<br />
DESCRIPTION OF TOPIC:<br />
To realize successful malting process yielding<br />
satisfactory malt, it is necessary to adapt <strong>the</strong><br />
malting conditions to <strong>the</strong> changes in <strong>the</strong> quality<br />
of raw material. This adaptation is very<br />
time and money consuming and must be repeated<br />
every year as climate conditions and<br />
site infl uence barley quality.<br />
In this project, <strong>the</strong> quality of malt has been assessed<br />
using Mid-InfraRed spectroscopy. The<br />
quality of a wide variety of barley has been<br />
studied 394 samples, including 3 harvest<br />
years, winter and spring barley, 77 barley<br />
varieties, collected on 16 cultivation places.<br />
Conventional barley quality index (Moisture<br />
content, Protein, Betaglucan) was determined.<br />
The database thus depicts a large and<br />
unique range of barley quality.<br />
The results indicate that MIR spectrometry<br />
could be a very usefull and rapid analytical<br />
tool to assess malting barley quality. It allows<br />
barley classifi cation according to harvest<br />
year and quality.<br />
Budour Ajib<br />
AJIB Budour<br />
Mail: Budour.ajib@univ.lorraine.fr.<br />
Phone : 0033383595769<br />
Université de Lorraine (UDL)/ École nationale<br />
supérieure d‘agronomie et des industries alimentaires<br />
(ENSAIA) / Laboratoire Réactions<br />
Génie des Procédés (LRGP-UMR 7274) / CNRS<br />
Equipe Bioprocédés - Biomolécules 2, avenue<br />
de la <strong>for</strong>êt de Haye - TSA 40602, 54518 - VAN-<br />
DOEUVRE CEDEX. PhD student in Biotechnologies<br />
Process. Thesis subject :( studying and<br />
modeling barley during malting process)<br />
46<br />
P 008<br />
Microwave superheated water<br />
extraction of brewers´ spent grain<br />
arabinoxylans<br />
Tiago Brandão 2, Elisabete Coelho 1,<br />
Mariana Pinto 1, Tiago Brandão 2,<br />
Manuel A. Coimbra 1<br />
1Universidade de Aveiro, Department of Chemistry, Aveiro,<br />
Portugal,<br />
2Unicer Bebidas, SA, Porto, Portugal<br />
DESCRIPTION OF TOPIC:<br />
Brewers‘ spent yeast (BSY) is <strong>the</strong> second<br />
major by-product of brewery industry. These<br />
residues comprise polysaccharides, namelyglucans<br />
and mannoproteins, which should be<br />
recovered due to <strong>the</strong>ir bioactive properties.<br />
In order to solubilize all <strong>the</strong>se polysaccharides,<br />
a sequential extraction with hot water<br />
and alkali solutions from 0.1M to 8M of KOH,<br />
followed by a partial acid hydrolysis was per<strong>for</strong>med.<br />
The 4M KOH solubilized 6% of <strong>the</strong> carbohydrates,<br />
corresponding to 26% of <strong>the</strong> total mannoproteins<br />
and 1% of glucans. The 8M KOH<br />
solubilized 18% of <strong>the</strong> carbohydrates, corresponding<br />
to 1% of total mannoproteins and<br />
22% of glucans. The glucans present in <strong>the</strong><br />
residue left were solubilized by partial acid<br />
hydrolysis. Using this procedure, BSY can be<br />
a source of cold water soluble polysaccharides<br />
able to be used as food ingredients to be<br />
incorporated in aqueous matrices as dietary<br />
fi ber.<br />
We thank FCT <strong>for</strong> fi nancial support of Research<br />
Unit 62/94-QOPNA and post doctoral<br />
grant SFRH/BPD/70589/2010.<br />
Tiago Brandão<br />
Tiago Brandao: Education: MSc Biotechnology<br />
– Leicester University, Biochemistry – Porto<br />
University, Brewmaster – Scandinavian<br />
School of Brewing. Current Positions: Innovation,<br />
Research & NPD Manager at Unicer Bebidas,<br />
SA since 2007, Executive Board member<br />
at Maltibérica, SA since 2007
P 009<br />
Eff ect of brewing process on phenolic<br />
compounds and <strong>the</strong>ir corresponding<br />
antioxidant activities<br />
Celine Clayeux 1, Damien Steyer 1,<br />
Luc Didierjean 2, Saïd Ennahar 3<br />
1TWISTAROMA, Colmar, France, 2Carlsberg Group, Strasbourg,<br />
France, 3Université de Strasbourg, Strasbourg, France<br />
DESCRIPTION OF TOPIC:<br />
Phenolic content of extracts and <strong>the</strong>ir corresponding<br />
antioxidant activities at diff erent<br />
stages of beer processing were investigated<br />
using a liquid chromatography online antioxidant<br />
detection method.<br />
Ethyl acetate extracts of barley, malt, wort<br />
and beer were separated using reverse phase<br />
HPLC and compounds eluting from <strong>the</strong><br />
column were submitted to two UV-visible detections:<br />
one <strong>for</strong> <strong>the</strong> phenolic compounds; and<br />
<strong>the</strong> o<strong>the</strong>r <strong>for</strong> <strong>the</strong> reduced <strong>for</strong>m of <strong>the</strong> radical<br />
cation ABTS.+ (CAS 30931-67-0).<br />
Prodelphinidin B3, procyanidin B3 and catechin<br />
were identifi ed as three major contributors<br />
in <strong>the</strong> antioxidant activity of barley.<br />
Malting had a dramatic impact resulting in a<br />
decrease in <strong>the</strong>ir amounts and <strong>the</strong>ir associated<br />
antioxidant activities. However, <strong>the</strong>ir contents<br />
increased during <strong>the</strong> brewing step and<br />
<strong>the</strong>n slightly decreased during hopping and<br />
fermentation.<br />
This study allowed <strong>the</strong> comparison of <strong>the</strong><br />
phenolic contents and <strong>the</strong> corresponding<br />
antioxidant profi les of barley, malt, wort and<br />
beer.<br />
Celine Clayeux<br />
Head of research and innovation at Twistaroma<br />
(2011-) - PhD in fi eld of analytical<br />
chemistry at University of Strasbourg and<br />
Carlsberg Group, Brasseries Kronenbourg<br />
(2008-2011)<br />
P 010<br />
Potential plant protecting substances<br />
from hops in dry-hopped beer<br />
Annika Lagemann 1, Stefan Hanke 1,<br />
Matthias Kippenberger 1, Georg Stettner 1<br />
1Bitburger Braugruppe GmbH, Bitburg, Germany<br />
DESCRIPTION OF TOPIC:<br />
Since 2012, <strong>the</strong> traditionally established<br />
method of dry-hopping has been offi cially<br />
approved <strong>for</strong> beer brewing in Germany. This<br />
possibility led to a quick increase of <strong>the</strong> percentage<br />
of strongly hopped beer varieties on<br />
<strong>the</strong> german market. The aim of dry-hopping<br />
is to transfer particular aroma compounds<br />
from <strong>the</strong> hop to <strong>the</strong> fi nished brew. So far t<strong>here</strong><br />
seem to have been no investigations on unwanted<br />
hop-specifi c substances which could<br />
be additionally transfered. T<strong>here</strong><strong>for</strong>e we investigated<br />
a possible contamination of beer<br />
with low-MRL pesticides used in hop cultivation,<br />
with particular attention being paid to<br />
myclobutanil and quinoxyfen. For our study,<br />
beers were produced with diff erent hop additions<br />
(200 to 650 g/hl). Finally, residues of different<br />
pesticides were measured in <strong>the</strong> fi nal<br />
product. It could be shown that myclobutanil<br />
migrate into <strong>the</strong> beer and reach values close<br />
to <strong>the</strong> legal limit. This suggests that pesticide<br />
levels in strongly hopped kinds of beer should<br />
be closely monitored.<br />
Annika Lagemann<br />
Laboratory manager, Bitburger Braugruppe<br />
GmbH, Bitburg, Germany (July 2011 to present)<br />
Flavour scientist, Bitburger Braugruppe<br />
GmbH, Bitburg, Germany (September 2008 to<br />
June 2011) Scientifi c assistant, Chair of Food<br />
Chemistry and Molecular Sensory Science,<br />
TUM, Freising, Germany (Oktober 2007 to July<br />
2008) Scientifi c assistant, Institute of Food<br />
Chemistry, WWU, Münster, Germany (May<br />
2005 to September 2007) Study of Food Chemistry,<br />
University of Bonn and CVUA Münster<br />
(1998-2005)<br />
P 011<br />
Protein-thiols in beer and throughout<br />
<strong>the</strong> brewing process<br />
Signe Hoff 1, Marianne N Lund 1,<br />
Bir<strong>the</strong> M Jespersen 1, Mogens L Andersen 1<br />
1Copenhagen University, Food Science, Frederiksberg,<br />
Denmark<br />
DESCRIPTION OF TOPIC:<br />
Protein-thiols have been suggested to be important<br />
antioxidants infl uencing <strong>the</strong> oxidative<br />
stability of beer. A method <strong>for</strong> quantifi cation<br />
of total soluble protein-derived thiols in wort<br />
and beer has been developed based on HPLC<br />
separation and detection of fl uorescent adducts<br />
with <strong>the</strong> maleimide compound<br />
ThioGlo 1.<br />
The amounts of protein-thiols varied between<br />
barley varieties. Increased thiol oxidation<br />
were linked to a lowering of wort fi ltration<br />
rates. Sweet wort was found to contain compounds<br />
able to oxidize protein-thiols. These<br />
thiol-oxidizing compounds decreased in activity<br />
through increased malt roasting; and<br />
wort boiling eliminated <strong>the</strong>ir activity, suggesting<br />
an enzymatic origin of <strong>the</strong> eff ect.<br />
Thiols have been quantifi ed in beer in various<br />
concentrations and correlated to <strong>the</strong> oxidative<br />
stability of <strong>the</strong> beers. The levels of thiols were<br />
found to decease during storage, suggesting<br />
that thiols are intermediates in redox reactions<br />
during beer staling.<br />
Signe Hoff<br />
Currently a PhD student at University of<br />
Copenhagen with focus on oxidative stability<br />
of wort and beer. Bsc in Food Engineering<br />
(2006) and Msc in Food Science and Technology<br />
(2010).<br />
47 34 TH EBC CONGRESS · LUXEMBOURG 26 – 30 MAY 2013
P 012<br />
Analysis of fl avor active carbonyls<br />
in beer by derivatization with 2,4-dinitrophenylhydrazine<br />
and HPLC-<br />
ESI-MS/MS<br />
Nina Baumjohann 1, Diedrich Harms 1<br />
1VLB Berlin e.V., Central Laboratory, Berlin, Germany<br />
DESCRIPTION OF TOPIC:<br />
During beer aging plenty of fl avor active<br />
carbonyls are <strong>for</strong>med by diff erent degradation<br />
processes. Due to <strong>the</strong>ir very low concentrations<br />
aff ecting <strong>the</strong> quality of beer (e.g.<br />
2-(E)-nonenal) a sensitive analysis method is<br />
needed. The aim of this work is to develop a<br />
robust option <strong>for</strong> <strong>the</strong> determination of fl avor<br />
active carbonyls as addition or alternative to<br />
laborious GC-MS routine analysis methods.<br />
After a preliminary matrix separation, a selective<br />
derivatization of carbonyls is eff ected<br />
by <strong>the</strong> use of <strong>the</strong> reagent 2,4-Dinitrophenylhydrazine<br />
(2,4-DNPH). For a sensitive determination<br />
of <strong>the</strong> <strong>for</strong>med derivatives high per<strong>for</strong>mance<br />
liquid chromatography coupled with<br />
electrospray-ionization and tandem mass<br />
spectrometry (HPLC-ESI-MS/MS) is used. By<br />
applying <strong>the</strong> multiple reaction monitoring<br />
(MRM) <strong>the</strong> selectivity is increased. Additionally,<br />
unexpected or unknown carbonyls can<br />
be discovered by <strong>the</strong> use of diff erent mass<br />
spectrometric experiments.<br />
Nina Baumjohann<br />
Nina Baumjohann studied food chemistry<br />
at <strong>the</strong> University of Münster and graduated<br />
as a certifi ed food chemist. Since 2010 she<br />
has been working as a scientifi c assistant in<br />
<strong>the</strong> Central Laboratory of <strong>the</strong> Research and<br />
Teaching Institut <strong>for</strong> Brewing in Berlin.<br />
P 013<br />
Evaluation of chemical physical<br />
stability of craft beers through<br />
unconventional methods<br />
Stefano Bertoli 1, Stefano Buiatti 1,<br />
Paolo Passaghe 1<br />
1University of Udine, Department of Food Science, Udine, Italy<br />
DESCRIPTION OF TOPIC:<br />
Aims: The aim of this work was to evaluate<br />
<strong>the</strong> colloidal stability of beers correlating EBC<br />
methods (sensitive proteins and Chapon test)<br />
to gluten content and antioxidant activity (AA).<br />
Methods: Three beers with diff erent <strong>for</strong>mulations<br />
were produced: <strong>the</strong> reference one 100%<br />
malted barley, <strong>the</strong> o<strong>the</strong>r two with increasing<br />
amounts (20 and 40%) of buckwheat. Chemical<br />
physical stability was evaluated using four<br />
indices: sensitive proteins, alcohol chill haze<br />
test, gluten content and antioxidant activity. Ttest<br />
was applied <strong>for</strong> statistical analysis.<br />
Results: Both gluten analysis and antioxidant<br />
activity measurements showed a correlation<br />
with data obtained with EBC methods.<br />
Conclusions: The analysis of gluten and AA<br />
may be used as qualitative and predicitive<br />
indexes of stability being more specifi c than<br />
sensitive proteins and Chapon test. The use of<br />
gluten free adjuncts could lead to more stable<br />
fi nal products with a gluten content less than<br />
100 mg/L.<br />
Stefano Bertoli<br />
Stefano Bertoli was born in Udine in 1982,<br />
he got <strong>the</strong> degree in Food Science and Technology<br />
at <strong>the</strong> University of Udine, Italy. After<br />
some experience as a brewer, gained in Italy<br />
and abroad, he is now working with Prof.<br />
Buiatti at <strong>the</strong> Department of Food Science as<br />
brewmaster and coordinator of <strong>the</strong> activities<br />
of beer production and on <strong>the</strong> optimization of<br />
production techniques of craft beer and evaluation<br />
of chemical and sensory properties of<br />
fi nished product. In addition, he serves as brewery<br />
manager at <strong>the</strong> Birrifi cio Udinese.<br />
48<br />
P 014<br />
System and method <strong>for</strong> on-line<br />
monitoring of beer primary fermentation<br />
based on UV-VIS-SWNIR<br />
spectroscopy<br />
Pedro Rodrigues 1, Tiago Brandão 1, Rui M.C.<br />
Martins 2,3, José A. Teixeira 3, Eurico Seabra 4,<br />
Luis F.F. Silva 4, Antonio A. Vicente 3<br />
1Unicer Bebidas, SA, S. Mamede Infesta, Portugal, 2Universidade<br />
do Minho, CBMA - Molecular Biology and Environmental<br />
Research Center, Braga, Portugal, 3Universidade do Minho,<br />
IBB - Institute <strong>for</strong> Biotechnology and Bioengineering, Centre<br />
of Biological Engineering, Braga, Portugal, 4Universidade do<br />
Minho, CT2M - Centre <strong>for</strong> Mechanical and Materials Technologies,<br />
Guimarães, Portugal<br />
DESCRIPTION OF TOPIC:<br />
Aim: This work aims at demonstrating a new<br />
system and method <strong>for</strong> on-line monitoring of<br />
beer primary fermentation.<br />
Methods: The system is based on UV-VIS-<br />
SWNIR spectroscopy. It includes: 1) a fi beroptic<br />
probe and a universal probe adaptor<br />
specially designed <strong>for</strong> industrial fermenters;<br />
2) a mini-spectrometer; 3) software specially<br />
developed <strong>for</strong> multivariate calculation of fermentation<br />
parameters; 4) graphical interface.<br />
Results: Calibrations were developed <strong>for</strong> biomass<br />
(CFU/mL), Brix, pH, ethanol (%), color,<br />
apparent and real extract (%), RDF (%), ADF<br />
(%), n-propanol (mg/L), iso-butanol (mg/L), total<br />
amyl alcohols (mg/l), ethyl acetate (mg/L),<br />
amyl acetate (mg/L), acetaldehyde (mg/L),<br />
dimethyl-sulfate (mg/L); diacetyl (mg/L).<br />
Correlation factors were always above 0.92.<br />
On-line calculation of <strong>the</strong>se parameters was<br />
per<strong>for</strong>med.<br />
Conclusions: The system is able to per<strong>for</strong>m<br />
real-time and predictive control and diagnostics<br />
of beer fermentations, allowing preventive<br />
or corrective measures.<br />
Pedro Rodrigues<br />
Pedro Rodrigues was born at Oporto, in 1973.<br />
In 1996 took <strong>the</strong> degree in Biochemistry and<br />
in 2003 a PhD. in Chemistry, both in Oporto<br />
University. From 1996 to 2004, works in research<br />
within Oporto University, and in 2001<br />
at BRI, w<strong>here</strong> builds up his passion <strong>for</strong> beer.<br />
Since 2004 works <strong>for</strong> Unicer, passing through<br />
diff erent positions in Quality & Innovation<br />
Dept. as manager, fi rst in <strong>the</strong> central lab, in<br />
central technical services and fi nally in industrial<br />
quality. In 2011 was appointed as production<br />
director.
P 015<br />
Comparing optical versus traditional<br />
measurement technology in <strong>the</strong><br />
brewery<br />
Daniel L Gore 1, Keyvan Ghanaviztchi 1<br />
1Anton Paar GmbH, Process Instrumentation, Graz, Austria<br />
DESCRIPTION OF TOPIC:<br />
Optical measurement technology is becoming<br />
more and more common in process environments<br />
and off ers many benefi ts, such as ease<br />
of installation and cleaning, compact size and<br />
reduced maintenance, but does it also meet<br />
<strong>the</strong> high expectations of quality personnel in<br />
terms of accuracy, repeatability and stability?<br />
This poster will show <strong>the</strong> results of a head to<br />
head test comparison <strong>for</strong>: ease of use, measurement<br />
per<strong>for</strong>mance (accuracy and repeatability)<br />
compared to <strong>the</strong> lab reference, per<strong>for</strong>mance<br />
during product changes and start<br />
up and response time. The test location was<br />
a 60.000hl brewery with 14 diff erent beers<br />
and multiple, daily product changes. The test<br />
candidates were an optical, mid-infrared ATR<br />
(attenuated total refl ectance) spectrometer<br />
and <strong>the</strong> classical beer monitor combining<br />
density and sound velocity with a separate<br />
CO 2 measurement using <strong>the</strong> Volume Expansion<br />
Method.<br />
Daniel L Gore<br />
Daniel Gore received his B.A from <strong>the</strong> University<br />
of Maryland in 1995. After graduating he<br />
began an apprenticeship as brewer and malter<br />
in Germany and worked at <strong>the</strong> Lammbrauerei<br />
Hilsenbeck, Uerige Obergärige Hausbrauerei<br />
and Quenzer Bräu and as Head Brewer at<br />
<strong>the</strong> Long Trail Brewing Company. In 2006 he<br />
moved to Anton Paar USA and in 2010 moved<br />
to Austria as Anton Paar‘’s Application Specialist<br />
to support existing applications in <strong>the</strong><br />
beverage industry and develop new applications<br />
and technologies.<br />
P 016<br />
Monitoring of <strong>the</strong> antioxidant potential<br />
of beer<br />
Edyta Kordialik-Bogacka 1, Anna Diowksz 1<br />
1Lodz University of Technology, Institute of Fermentation<br />
Technology and Microbiology, Lodz, Poland<br />
DESCRIPTION OF TOPIC:<br />
The quality of a beer brand should be consistent,<br />
despite its production in diff erent localizations.<br />
The antioxidant potential of <strong>the</strong><br />
beer brand produced in diff erent breweries<br />
was evaluated. Since this brand was produced<br />
as pasteurized and non-pasteurized beer,<br />
<strong>the</strong> infl uence of pasteurization process on<br />
<strong>the</strong> antioxidant activity of beer was also assessed.<br />
Moreover, antioxidant properties of<br />
<strong>the</strong> products after <strong>the</strong>ir storage at 22°C <strong>for</strong> 4<br />
weeks were examined. The antioxidant capacity<br />
of beer was measured both by <strong>the</strong> ferric<br />
reducing antioxidant power (FRAP) assay and<br />
<strong>the</strong> radical scavenging assay (DPPH). The antioxidant<br />
activity of <strong>the</strong> beer brand showed<br />
signifi cant diff erences depending on its origin.<br />
When DPPH assay was used a signifi cant<br />
decrease in <strong>the</strong> antioxidant activity of nonpasteurized<br />
beer was observed after storage.<br />
Fur<strong>the</strong>rmore, <strong>the</strong> changes in antioxidant properties<br />
of oat and barley beer were compared.<br />
A lower antioxidant potential was measured<br />
in <strong>the</strong> product from oat.<br />
Edyta Kordialik-Bogacka<br />
EDYTA KORDIALIK-BOGACKA has worked at<br />
<strong>the</strong> Institute of Fermentation Technology and<br />
Microbiology of Lodz University of Technology<br />
since 1995. She obtained her PhD degree, studying<br />
foaming ability of brewing yeast Saccharomyces<br />
cerevisiae. Meanwhile she spent<br />
nine months at Heriot-Watt University. Her recent<br />
research has been devoted towards lowalcoholic<br />
oat beverages, physiological activity<br />
of yeast and yeast immobilization. She is a<br />
member of <strong>the</strong> EBC Brewing Science Group.<br />
P 016a<br />
Radical scavenging ability of protein<br />
and peptide thiols in beer<br />
Marianne Lund 1, Natália E.C. de Almeida 2,<br />
Daniel R Cardoso 2, Mogens L Andersen 1<br />
1University of Copenhagen, Department of Food Science, Frederiksberg<br />
C, Denmark, 2University of São Paulo, Chemistry<br />
Institute, São Carlos, Brazil<br />
DESCRIPTION OF TOPIC:<br />
The antioxidative capacity of thiol-containing<br />
peptides and proteins (P-SH) in beer was evaluated<br />
by a kinetic study of <strong>the</strong>ir ability to scavenge<br />
<strong>the</strong> 1-hydroxyethyl radical (HER), which<br />
is known to be <strong>the</strong> main radical species <strong>for</strong>med<br />
during beer aging processes. A number<br />
of P-SHs was investigated, among which were<br />
glutathione and LTP1 (Lipid Transfer Protein<br />
1), an abundant beer protein. The reactivity of<br />
<strong>the</strong> P-SHs towards HER was evaluated using<br />
a competitive kinetic approach, employing <strong>the</strong><br />
spin-trap POBN as a probe and by using Electron<br />
Paramagnetic Resonance (EPR) to detect<br />
<strong>the</strong> HER-POBN spin adduct.<br />
All investigated P-SHs were very reactive towards<br />
HER with apparent second-order rate<br />
constants close to <strong>the</strong> diff usion limit and ranging<br />
from 0.5 to 6.1 109 L mol-1 s-1 with LTP1<br />
having <strong>the</strong> highest rate constant. The rates of<br />
scavenging HER by P-SH are competitive with<br />
<strong>the</strong> degradation of hops bitter acids in beer<br />
and likely to protect against oxidation mediated<br />
by HER.<br />
Marianne Lund<br />
Marianne Lund, PhD, Associate professor. She<br />
received a MSc in food science and technology<br />
in 2003 from University of Copenhagen<br />
(UCPH) and a PhD degree in 2007 based on<br />
studies of protein oxidation in meat. In 2008<br />
she began as a post doc working with oxidation<br />
in beer and <strong>the</strong> infl uence of thiol groups<br />
and was appointed associate professor in<br />
2012. She now works in collaboration with<br />
Novozymes on fl avor stability of beer. She has<br />
published 23 peer-reviewed scientifi c papers.<br />
49 34 TH EBC CONGRESS · LUXEMBOURG 26 – 30 MAY 2013
P 017<br />
The use of confocal laser scanning<br />
microscope (CLSM) <strong>for</strong> determination<br />
of fi ltration inhibiting substances<br />
in kieselguhr and membrane<br />
fi ltration<br />
Michael Kupetz 1, Martin Zarnkow 1,<br />
Thomas Becker 1<br />
1TUM Weihenstephan, Lehrstuhl für Brau- und Getränketechnologie,<br />
Freising, Germany<br />
DESCRIPTION OF TOPIC:<br />
The fi ltration process of beer is enormously<br />
infl uenced by reversible and irreversible blockages.<br />
The aim of this research is to stain<br />
and identify <strong>the</strong>se components by CLSM. Staining<br />
components were used which only react<br />
with specifi c beer ingredients. The fi ltration<br />
was per<strong>for</strong>med on an automated laboratory<br />
membrane, as well as kieselguhr precoat fi lter.<br />
A combination of <strong>the</strong> dyes propidiumiodide,<br />
fuchsine-acid and schiff ´s reagent made<br />
it possible to distinguish polysaccharides<br />
(α-/β-glucans), proteins and yeast cells. The<br />
staining was per<strong>for</strong>med by fi ltration of <strong>the</strong><br />
dyes through <strong>the</strong> sample. Conclusions of <strong>the</strong><br />
particles could take on <strong>the</strong> basis of <strong>the</strong> available<br />
wavelengths of <strong>the</strong> lasers. These allowed<br />
identifying <strong>the</strong> blockages of <strong>the</strong> surface layer<br />
and <strong>the</strong> interior of <strong>the</strong> fi lter medium.<br />
Michael Kupetz<br />
Michael Kupetz graduated from Technische<br />
Univeristät Müchnen in 2011 as a degreed<br />
engineer <strong>for</strong> brewing sciences and beverage<br />
technology. In 2012 he started his doctoral<br />
<strong>the</strong>sis with Prof. Thomas Becker at <strong>the</strong> institute<br />
of brewing science and beverage technology.<br />
His research project is <strong>the</strong> ‚Comparative<br />
identifi cation of inhibitory substances in <strong>the</strong><br />
membrane and diatomaceous earth fi ltration<br />
of beer‘<br />
P 018<br />
Beta-glucan hazes - what‘s <strong>the</strong><br />
problem?<br />
Cecilia Damiani 1, Karin Pawlowsky 1,<br />
Giuseppe Perretti 2<br />
1Campden BRI, Nutfi eld, United Kingdom, 2Università di<br />
Perugia, Perugia, Italy<br />
DESCRIPTION OF TOPIC:<br />
In some instances beer haze is caused by<br />
beta-glucan molecules. Although this carbohydrate<br />
haze has commonly been associated<br />
with poor malt quality or mashing problems,<br />
it can also originate from <strong>the</strong> yeast cell wall.<br />
Indeed, with <strong>the</strong> increased use of centrifuges<br />
<strong>for</strong> yeast separation and high gravity brewing,<br />
yeast cells can be exposed to stresses<br />
resulting in damage to cell walls and release<br />
of beta-glucans. Until recently, t<strong>here</strong> was no<br />
simple test to distinguish between a yeast<br />
and malt beta-glucan haze, which has made<br />
trouble shooting <strong>the</strong> haze problem diffi cult.<br />
However, we have now solved this technical<br />
challenge and describe <strong>here</strong> a novel protocol<br />
<strong>for</strong> <strong>the</strong> isolation/examination of beta-glucan<br />
hazes and determination of whe<strong>the</strong>r <strong>the</strong>y originate<br />
from yeast or barley.<br />
Cecilia Damiani<br />
Cecilia Damiani graduated at <strong>the</strong> University<br />
of Perugia (Italy) in 2012, having obtained a<br />
B.Sc. in Food Science and Technology and a<br />
M.Sc. in Food Technology and Biotechnology.<br />
During her studies, she completed her Master<br />
<strong>the</strong>sis at Campden BRI (UK) working on beta-glucan<br />
haze in beer. After defending her<br />
<strong>the</strong>sis at <strong>the</strong> University of Perugia, she has<br />
been working as a scientist at Campden BRI<br />
in <strong>the</strong> Analytical department since August<br />
2012, carrying out chemical analyses on beer.<br />
50<br />
P 019<br />
Changes of metal concentration in<br />
beer production and <strong>the</strong>ir Infl uence<br />
on <strong>the</strong> mechanism <strong>for</strong> haze <strong>for</strong>mation<br />
Annika Burmeister 1, Adelina Calean 2,<br />
Thomas Kunz 3, Frank-Jürgen Methner 3,<br />
Peter Winterhalter 1, Peter Fleischmann 1<br />
1TU Braunschweig, Institute of Food Chemistry, Braunschweig,<br />
Germany, 2TU Braunschweig, Institute Geoecology,<br />
Braunschweig, Germany, 3TU Berlin, Institute of<br />
Biotechnology, Berlin, Germany<br />
DESCRIPTION OF TOPIC:<br />
Chill haze in beer causes an economical loss<br />
<strong>for</strong> <strong>the</strong> brewing industry. Due to <strong>the</strong> precipitation<br />
of protein-polyphenol-metal complexes<br />
under inadequate storage conditions, consumers<br />
confound <strong>the</strong>se hazes with mold and its<br />
associated health risk.<br />
This work deals with <strong>the</strong> development of<br />
concentrations of Mn, Cu, Zn and Fe in beer<br />
raw materials, beers and <strong>the</strong>ir fi nal concentrations<br />
in isolated beer hazes (analyzed via<br />
ICP-MS and ICP-OES). Independent from <strong>the</strong><br />
raw materials used <strong>the</strong> concentrations of Fe,<br />
Mn, Cu and Zn in beers strongly depend on<br />
<strong>the</strong> steps of brewing process. Metal concentrations<br />
in <strong>the</strong> resulting hazes are 17 fold higher<br />
<strong>for</strong> Fe, 4 fold higher <strong>for</strong> Cu, 6 fold higher<br />
<strong>for</strong> Zn and 30 fold higher <strong>for</strong> Mn than in <strong>the</strong><br />
original beers.<br />
Annika Burmeister<br />
*10/2002 - 11/2007 Studies of Food Chemistry<br />
at Goe<strong>the</strong> Universtiy Frankfurt/main,<br />
Germany and Wilhelms University, Muenster,<br />
Germany *12/2007 - 6/2009 Scientifi c<br />
Researcher, Institute of Food Chemistry, Research<br />
Group of Prof. Dr. Humpf, Wilhelms<br />
University, Muenster,Germany *since 7/2009<br />
PhD Student, Institute of Food Chemistry, Research<br />
Group of Prof. Dr. Winterhalter and Dr.<br />
Fleischmann, TU Braunschweig, Germany
P 020<br />
Improving your draught beer quality<br />
Johannes Tippmann 1, Simon Henke 2,<br />
Heinz Dauth 3, Thomas Becker 1<br />
1Technische Universität München, Lehrstuhl für Brau- und<br />
Getränketechnologie, Freising, Germany, 2Technische Universität<br />
München, Lehrstuhl für Verfahrenstechnik Disperser<br />
Systeme, Freising, Germany, 3Hochschule Ansbach, Fakultät<br />
Ingenieurwissenschaften, Ansbach, Germany<br />
DESCRIPTION OF TOPIC:<br />
For years, people talk about <strong>the</strong> quality of<br />
draught beer and <strong>the</strong>ir potential <strong>for</strong> improvement.<br />
Developments in dispensing systems<br />
are, however, in most cases, just to improve<br />
<strong>the</strong> appearance of <strong>the</strong> equipment. The most<br />
important aspect <strong>for</strong> a brewer - hygiene - is<br />
often neglected. As a consequence, <strong>the</strong> quality<br />
of beer is destroyed in <strong>the</strong> last few meters,<br />
just be<strong>for</strong>e <strong>the</strong> consumer enjoys it.<br />
We carried out extensive studies on construction,<br />
cleaning and maintenance of dispensing<br />
equipment. It was found <strong>here</strong> that <strong>the</strong> microbial<br />
load in draught beer could be reduced by<br />
good hygiene practices in 6 weeks by more<br />
than 90%. The investigation of design aspects<br />
also brought strong improvements. Here also<br />
immense hygienic improvements were observed.<br />
The presentation shows <strong>the</strong>se fi ndings and<br />
gives advice, such as fast recontaminations<br />
can be avoided and <strong>the</strong> draught beer quality<br />
can be improved. The presentation also will<br />
show <strong>the</strong> growth and sensorial infl uence of<br />
microbial infections in draught beer.<br />
Johannes Tippmann<br />
Johannes Tippmann graduated from university<br />
in 2004 as a diploma engineer <strong>for</strong> brewing<br />
sciences and beverage technology. In 2005 he<br />
started his PhD <strong>the</strong>sis with Prof. Sommer at<br />
<strong>the</strong> Lehrstuhl für Verfahrenstechnik disperser<br />
Syteme, TU München, about solid handling<br />
in <strong>the</strong> brewhouse. In 2012 he changed his<br />
affi liation and is now working as group leader<br />
<strong>for</strong> <strong>the</strong> work group “brew house processing<br />
and dispense systems“ at <strong>the</strong> Lehrstuhl für<br />
Brau- und Getränketechnologie, TU München.<br />
P 021<br />
Slow and fast CO 2 escape from beer<br />
in relation to gushing<br />
Jan Savel 1, Petr Kosin 1, Adam Broz 2<br />
1Budejovicky Budvar, n.p., Research&Development,<br />
C.Budejovice, Czech Republic, 2Budejovicky Budvar, n.p.,<br />
Production Director, C.Budejovice, Czech Republic<br />
DESCRIPTION OF TOPIC:<br />
Gas volume (GV) of CO 2 liberated from beer<br />
was measured with simple buoyant gasometer.<br />
The beer volume displaced (DV) above<br />
<strong>the</strong> bottle rim was also measured using <strong>the</strong><br />
syringe with graduated scale. Gas volume of<br />
CO 2 escaping from <strong>the</strong> open bottle after its<br />
opening depended on beverage temperature,<br />
kind of beer, its shelf time and <strong>the</strong> presence of<br />
ano<strong>the</strong>r gas e.g air. GV and DV strongly increased<br />
after <strong>the</strong> addition of dry porous materials<br />
such as diatomaceous earth, boiling stone,<br />
active charcoal, cellulose powder and both a<br />
little decreased by <strong>the</strong>ir wetting. Spontaneous<br />
liberation of carbon dioxide from beer and<br />
saturated water depended on kind of solid<br />
particles and gases bound on <strong>the</strong>ir surfaces.<br />
The addition of small particles of cellulose to<br />
carbonated liquids increased GV and DV from<br />
beer and carbonated water although saturated<br />
water did not contain any hydrophobins or<br />
surfactants. T<strong>here</strong> is a speculation that cellulose<br />
particles could originate from grain wall<br />
destroyed by fungi.<br />
Jan Savel<br />
Jan Savel studied at <strong>the</strong> Institute of Chemical<br />
Technology, Prague, graduating with a Ph.D.<br />
degree. Currently, Dr. Savel is an external associate<br />
professor at <strong>the</strong> Institute of Chemical<br />
Technology, Prague, as well as head of <strong>the</strong><br />
Research Department at Budejovicky Budvar<br />
<strong>Brewery</strong>, N.C. Dr. Savel has been a member of<br />
<strong>the</strong> EBC Brewing Science Group since 1994.<br />
He has published more than 100 articles in<br />
Czech and <strong>for</strong>eign professional magazines,<br />
as well as a monograph dealing with brewing<br />
microbiology.<br />
P 022<br />
Confounding expectations:<br />
Decoupling visual and fl avour<br />
properties of beer<br />
Paul Hughes 1<br />
1Heriot-Watt University, School of Life Sciences, Edinburgh,<br />
United Kingdom<br />
DESCRIPTION OF TOPIC:<br />
Traditional beer brewing raw materials and<br />
operations typically aff ects more than one<br />
property of beer. Thus brewing with speciality<br />
malts confers both colour and specifi c<br />
fl avours to <strong>the</strong> fi nal product. Such coupling<br />
has been solved with <strong>the</strong> availability of malt<br />
fl avours and colours, permitting independent<br />
manipulation of malt-derived fl avour<br />
and colour. Here, we report <strong>the</strong> <strong>the</strong> infl uence<br />
of colour perception on fl avour expectations.<br />
Aqueous extracts of speciality malts were ultrafi<br />
ltered to create colour and fl avour fractions.<br />
A range of beers were treated with both<br />
fractions at diff erent concentrations. Tasters<br />
assessed <strong>the</strong>m blind (blue glass) and in clear<br />
glass. T<strong>here</strong> was a clear impact of <strong>the</strong> colour<br />
on perceived fl avour profi le, but <strong>the</strong> presence<br />
of roasted fl avour in lager beers seemed independent<br />
of whe<strong>the</strong>r <strong>the</strong> colour was visible<br />
or not. Whilst this seemed to indicate broad<br />
acceptance of fl avour attributes in lager<br />
beers, t<strong>here</strong> was an optimum beyond which<br />
preferences were aff ected.<br />
Paul Hughes<br />
A trained chemist, Paul is currently Professor<br />
of Brewing and Distilling at Heriot-Watt University.<br />
He specialises in whisky maturation,<br />
all aspects of fi nal product quality, hop chemistry,<br />
sensory methodology/modelling and<br />
business planning <strong>for</strong> innovation. Paul consults<br />
widely in <strong>the</strong> food and drink sectors and<br />
has published widely.<br />
51 34 TH EBC CONGRESS · LUXEMBOURG 26 – 30 MAY 2013
P 023<br />
Beverage Antioxidative IndeX (BAX)<br />
- a benefi cial index number <strong>for</strong><br />
prognosis of <strong>the</strong> oxidative fl avour<br />
stability<br />
Christian Müller 1, Thomas Kunz 1,<br />
Frank-Jürgen Methner 1<br />
1Technische Universität Berlin, Department of Biotechnology,<br />
Lab of Brewing Science, Berlin, Germany<br />
DESCRIPTION OF TOPIC:<br />
A beer matrix dependent, linear correlation<br />
between SO 2 and endogenous antioxidative<br />
potential (EAP) measured by EPR off ers a<br />
new index number <strong>for</strong> prognosis of oxidative<br />
fl avour stability. The Beverage Antioxidative<br />
indeX provides additional in<strong>for</strong>mation about<br />
anti- and pro-oxidative properties of <strong>the</strong> beer<br />
matrix independent on SO 2. The BAX is aff ected<br />
by beer ingredients and gives in<strong>for</strong>mation<br />
about consumption rate of EAP and <strong>for</strong>mation<br />
of aging compounds during storage.<br />
Low pH improves oxidative stability caused<br />
by increasing iron-complex bonding power<br />
and refl ected in lower oxygen activation, radical<br />
generation and higher EAP/BAX-values.<br />
Iron entry by raw materials and fi ltration can<br />
deteriorate oxidative stability. Hop ingredients<br />
like -acids can reduce oxidative processes<br />
acting as chelating agent. Maillard intermediates<br />
with reductone/enediol structure <strong>for</strong>med<br />
during kilning and boiling decrease oxidative<br />
stability by acceleration of <strong>the</strong> Fenton system.<br />
Hence barley can improve BAX.<br />
Christian Müller<br />
Christian Mueller did his brewer’s apprenticeship<br />
from 1998, followed by an occupation as<br />
a brewer and assistant in <strong>the</strong> quality control<br />
at <strong>the</strong> Tuborg/Carlsberg <strong>Brewery</strong>, Mönchengladbach,<br />
Germany. Afterwards, he studied<br />
brewing technology at <strong>the</strong> Technische Universität<br />
Berlin from 2002 to 2008 including student<br />
jobs in several research projects. After<br />
qualifying his engineer degree he has been<br />
working as a scientifi c assistant at <strong>the</strong> department<br />
of brewing science at <strong>the</strong> TU Berlin.<br />
P 024<br />
New aspects of beer glass<br />
infl uences on beer taste<br />
Petr Kosin 1, Jan Savel 1, Adam Broz 2<br />
1Budeweiser Budvar, N.C., R&D, Ceske Budejovice, Czech<br />
Republic, 2Budeweiser Budvar, N.C., Production, Ceske Budejovice,<br />
Czech Republic<br />
DESCRIPTION OF TOPIC:<br />
It is generally accepted that each beer brand<br />
should have its own specifi c glass, which fi ts<br />
to its marketing positioning and which supports<br />
perceived quality of beer brand. It is<br />
known that specifi c shapes of glass rim infl uence<br />
<strong>the</strong> rate and turbulences of beer fl ow to<br />
oral cavity and can support or suppress <strong>the</strong><br />
sensation of fl avor compounds. However <strong>the</strong><br />
infl uence of glass shape on <strong>the</strong> CO 2 release at<br />
dispensing has not been studied yet. We have<br />
shown in our previous papers that up to one<br />
half of <strong>the</strong> beer CO 2 content can be released<br />
at dispensing, which infl uences not only <strong>the</strong><br />
sparkling taste of beer, but also its drinkability,<br />
<strong>the</strong> perception of bitterness character and<br />
intensity and <strong>the</strong> perception of beer aromatic<br />
compounds. In this paper we will discuss <strong>the</strong><br />
connection of <strong>the</strong> shape of glass bottom and<br />
glass proportions with <strong>the</strong> CO 2 release at dispensing.<br />
These results can be useful <strong>for</strong> highlighting<br />
of beer specifi c sensory properties by<br />
proper designing of beer glass.<br />
Petr Kosin<br />
Petr Kosin received an engineering and Ph.D.<br />
degree in brewing and malting at <strong>the</strong> Institute<br />
of Chemical Technology Prague. He worked<br />
on both of his <strong>the</strong>ses “Application of Modern<br />
Methods <strong>for</strong> Yeast Activity Control in <strong>Brewery</strong>“<br />
and “Consumer perception of beer qualitative<br />
characteristics“ at Budweiser Budvar, N.C.<br />
in Ceske Budejovice. He has been working in<br />
research and development at Budweiser Budvar,<br />
N.C. since his graduation. He has been a<br />
member of EBC Brewing Science Group since<br />
2011.<br />
52<br />
P 025<br />
Development and validation of an<br />
assay method <strong>for</strong> volatile fl avour<br />
compounds in GMP beer fl avour<br />
standards<br />
Boris Gadzov 1, Mark Powell 2,<br />
Duncan Johnstone 2, Javier Gomez-Lopez 1,<br />
Dale Smith 1<br />
1FlavorActiV Limited, Chinnor, United Kingdom, 2Quay Pharmaceuticals<br />
Limited, Deeside, United Kingdom<br />
DESCRIPTION OF TOPIC:<br />
Flavour standards are a well-established means<br />
of training professional beverage sensory<br />
panels. The use of cyclodextrin-encapsulated<br />
fl avours provides a more representative and<br />
consistent sensory experience than raw fl avour<br />
material. Data from instrumental methods<br />
of analysis aff ord a more traceable and<br />
less subjective means of assuring fl avour<br />
standard quality and batch-to-batch consistency<br />
along with sensory evaluation. This<br />
study describes <strong>the</strong> development and validation<br />
of an assay method <strong>for</strong> six fl avour compounds<br />
(acetaldehyde, benzaldehyde, diacetyl,<br />
cis-3-hexenol, 2-methylpropionaldehyde<br />
and nonanal) encapsulated as beer fl avour<br />
standards. A headspace-GC-FID method was<br />
developed that was capable of extracting and<br />
separating all six compounds. The method‘s<br />
per<strong>for</strong>mance is considered to be more than<br />
adequate to control <strong>the</strong>se six compounds at<br />
concentrations relevant to <strong>the</strong>ir use as fl avour<br />
standards.<br />
Boris Gadzov<br />
Dr Boris Gadzov is Director of Global Sensory<br />
at FlavorActiV Limited in <strong>the</strong> UK. Dr Gadzov<br />
provides professional sensory training to<br />
taste panels throughout <strong>the</strong> world <strong>for</strong> FlavorActiV<br />
to many of <strong>the</strong> biggest brewers and<br />
beverage producers. Dr Gadzov holds a PhD in<br />
Food Molecular Microbiology and is a Doctor<br />
in Veterinary Medicine. A talented linguist, Dr<br />
Gadzov is also fl uent in a number of languages.
P 027<br />
A scientifi c look at <strong>the</strong> fl avour<br />
evolution of specialty beers<br />
Barbara Jaskula-Goiris 1, Brecht De<br />
Causmaecker 1, Tom Mertens 2, Luc De<br />
Cooman 1, Freddy Delvaux 2, Guido Aerts 1<br />
1KAHO St.-Lieven, Laboratory of Enzyme, Fermentation,<br />
and Brewing Technology (EFBT), Department Chemistry/<br />
Biochemistry - LFoRCe, Department M2S, KU Leuven, Gent,<br />
Belgium, 2KULeuven, Centre <strong>for</strong> Malting and Brewing Science,<br />
Department M2S, KU Leuven, Heverlee, Belgium<br />
DESCRIPTION OF TOPIC:<br />
The fl avour of beer alters upon storage. Due<br />
to <strong>the</strong> complexity of both malt and beer production<br />
as well as <strong>the</strong> intricate composition<br />
of <strong>the</strong> beer matrix, a multitude of parameters<br />
may have an eff ect on <strong>the</strong> fl avour stability of<br />
<strong>the</strong> fi nished product. Here we compared fl avour<br />
instability of pilsner beers with a wide<br />
variety of specialty beers. An increase in colour,<br />
haze and aldehydes content was observed<br />
independent of <strong>the</strong> beer style. With regard<br />
to <strong>the</strong> aldehyde content in <strong>the</strong> fresh and aged<br />
beers, great variations were observed in <strong>the</strong><br />
speciality beers, strongly depending on <strong>the</strong><br />
type and quality of <strong>the</strong> malt used. Pilsner<br />
beers show relatively low aldehyde contents<br />
in both fresh and aged beers when compared<br />
to specialty beers. However, despite of <strong>the</strong><br />
low aldehyde contents and somewhat better<br />
bitterness stability, pilsner beers were more<br />
prone to stale fl avour perception due to much<br />
lower masking eff ects of fermentation and<br />
hop derived fl avours.<br />
Barbara Jaskula-Goiris<br />
Barbara Jaskula-Goiris obtained her PhD in<br />
Biotechnology from Wroclaw University of<br />
Technology, Poland. Since 2008 she is working<br />
as scientist at <strong>the</strong> Laboratory of Enzyme, Fermentation<br />
and Brewing Technology of KAHO<br />
St.-Lieven in Gent, Belgium. The main fi elds of<br />
<strong>the</strong> research are beer fl avour stability, hop alpha-acids<br />
isomerisation, hop chemistry, and<br />
liquid chromatographic techniques.<br />
P 028<br />
Volatile compounds screening of<br />
14 commercial Alsatian beers by<br />
SBSE-LD-GC-MS<br />
Damien Steyer 1,2<br />
1TWISTAROMA, Colmar, France, 2INRA Colmar, Colmar, France<br />
DESCRIPTION OF TOPIC:<br />
Characterization of <strong>the</strong> volatile profi le of commercial<br />
beer is of interest <strong>for</strong> brewers. It allows<br />
to understand what diff erentiates <strong>the</strong>ir<br />
products from competitor. This can also be<br />
used as a tool <strong>for</strong> product development w<strong>here</strong><br />
compounds of interest could be measured<br />
and with appropriate modeling, <strong>the</strong>ir concentration<br />
enhanced during <strong>the</strong> production<br />
phases. Fourteen commercial beers from 3<br />
diff erent breweries (Kronenbourg, Meteor<br />
and Heineken) from Alsace were analyzed in<br />
duplicate by Stir Bar Sorptive Extraction Liquid<br />
Desorption-GC-MS. Statistical analysis of<br />
43 semi-quantifi ed volatile compounds enabled<br />
<strong>the</strong> separation of <strong>the</strong> beers into distinct<br />
groups. White beers was <strong>the</strong> most complex<br />
group with high level of β-damascenone and<br />
linalol, geraniol, citronellol w<strong>here</strong>as ales contained<br />
high level of ethyl caproate, ethyl phenylacetate<br />
as well as 4-vinylguaicol.<br />
Lagers could be separated in 2 groups<br />
according to <strong>the</strong> amounts of hop volatile<br />
compounds (linalol, α/β eudesmol, methyl<br />
geranate).<br />
Damien Steyer<br />
-Founder-Director of TWISTAROMA -PhD at<br />
<strong>the</strong> INRA de Colmar on <strong>the</strong> infl uence of yeast<br />
strain on <strong>the</strong> aromatic profi le of wine -Engineer<br />
in biotechnologie at <strong>the</strong> Ecole Supérieure<br />
de Biotechnologie de Strasbourg (ESBS)<br />
P 029<br />
Addition of fermentable and non<br />
fermentable carbohydrates - impact<br />
on <strong>the</strong> yeast metabolism, sweetness,<br />
palate fullness and SO 2-content<br />
in beer<br />
Torsten Seewald 1, Christof Reinhardt 1,<br />
Thomas Kunz 1, Frank-Jürgen Methner 1<br />
1Technische Universität Berlin, Lab of Brewing Science,<br />
Berlin, Germany<br />
DESCRIPTION OF TOPIC:<br />
Aim of this study was to investigate infl uences<br />
of fermentable and non-fermentable carbohydrate<br />
addition into brewing process prior<br />
fermentation to get a better insight in <strong>the</strong> infl<br />
uence on <strong>the</strong> yeast metabolism, sweetness,<br />
palate fullness and SO 2-<strong>for</strong>mation.<br />
Compared to <strong>the</strong> standard wort, <strong>the</strong> results<br />
demonstrate a general carbohydrate dependent<br />
increase in SO 2-<strong>for</strong>mation during<br />
fermentation. The highest increase in SO 2<br />
results from <strong>the</strong> fermentable sugars glucose<br />
and sucrose followed by <strong>the</strong> non-fermentable<br />
sugar isomaltulose and polydextrose. In correlation<br />
to <strong>the</strong> non-fermentable carbohydrate<br />
addition <strong>the</strong> additional SO 2-<strong>for</strong>mation mainly<br />
based on <strong>the</strong> increase in osmotic pressure is<br />
limited by <strong>the</strong> infl uences on <strong>the</strong> methionine<br />
pathway, <strong>the</strong> acetaldehyde production and <strong>the</strong><br />
activation of <strong>the</strong> sulphate-reductase.<br />
Dependent to <strong>the</strong> used sugar a sensory panel<br />
detected a reversal point w<strong>here</strong> <strong>the</strong> sweetness<br />
is coming to <strong>the</strong> <strong>for</strong>e and gets much<br />
stronger in comparison to <strong>the</strong> palate fullness.<br />
Torsten Seewald<br />
Torsten Seewald started his studies in food<br />
technology at <strong>the</strong> Technische Universität<br />
Berlin (TU Berlin) in October 2004. Since<br />
July 2009, he worked as a student assistant<br />
at <strong>the</strong> TU Berlin, Lab of Brewing Science and<br />
completed industrial placements at WILD and<br />
Herbstreith & Fox. After graduating in April<br />
2012, he is employed as a chartered engineer<br />
at <strong>the</strong> TU Berlin, Lab of Brewing Science. His<br />
research focus lies in analysing reaction mechanisms<br />
of carbohydrates and oxidative processes<br />
in beer.<br />
53 34 TH EBC CONGRESS · LUXEMBOURG 26 – 30 MAY 2013
P 030<br />
Investigation of <strong>the</strong> infl uence of<br />
a permease on diacetyl content<br />
during beer fermentation<br />
Nisha James 1, Christine Lang 2, Ulf Stahl 1<br />
1Research and Teaching Institute <strong>for</strong> Brewing in Berlin (VLB),<br />
Department of Microbiology, Berlin, Germany, 2TU Berlin,<br />
Berlin, Germany<br />
DESCRIPTION OF TOPIC:<br />
Yeasts secrete diacetyl during fermentation<br />
imparting an undesirable buttery fl avour to<br />
beer. Gene targets <strong>for</strong> reducing diacetyl production<br />
were identifi ed using transcriptome<br />
analysis. We identifi ed GAP1 as a potential<br />
candidate in several genes. Gap1p functions<br />
as a transporter <strong>for</strong> amino acids and previous<br />
studies have shown that <strong>the</strong> availability<br />
of amino acids <strong>for</strong> <strong>the</strong> cell infl uences diacetyl<br />
production. We verifi ed <strong>the</strong> transcriptome<br />
data by immunoblot analysis as Gap1p. We<br />
proposed <strong>the</strong> hypo<strong>the</strong>sis that t<strong>here</strong> is a positive<br />
correlation between amounts of Gap1p<br />
and diacetyl. To verify this we analysed diacetyl<br />
production under varying Gap1p levels in<br />
WT, Δgap1 and Gap1p overexpression strains<br />
of S. cerevisiae. We also show a positive correlation<br />
between Gap1p expression level<br />
and diacetyl levels under brewing conditions<br />
using industrial yeast strains. Our results indicate<br />
that infl uencing Gap1p levels indeed<br />
leads to changes in diacetyl levels.<br />
Nisha James<br />
Has a Masters in Biotechnolgy from TU-Hamburg.<br />
Currently pursuing PhD in <strong>the</strong> department<br />
Microbiology and Genetics, TU-Berlin.<br />
Has hands-on experience in various Molecular<br />
and Microbiology techniques, cloning and<br />
overexpression of biotechnologically relevant<br />
genes, bioin<strong>for</strong>matics, beer fermentation and<br />
analysis of various products using GC-MS,<br />
spectrophotometry.<br />
P 031<br />
A new insight <strong>for</strong> controlling <strong>the</strong><br />
hop aroma using hydrophobicity of<br />
yeast cell surface<br />
Toru Kishimoto 1, Kazuhiko Uemura 1,<br />
Masayuki Aizawa 1<br />
1ASAHI Breweries, Ltd., Research Laboratories <strong>for</strong> Brewing,<br />
Moriya-shi, Ibaraki, Japan<br />
DESCRIPTION OF TOPIC:<br />
The eff ects of cell surface hydrophobicity<br />
(CSH) of yeasts on <strong>the</strong> content of hop-derived<br />
hydrophobic compounds were investigated.<br />
The yeasts with high CSH have high fl occulation<br />
characteristics and are easily removed<br />
from <strong>the</strong> bottom of tank after fermentation.<br />
When <strong>the</strong> high CSH yeasts with high fl occulation<br />
characteristics are used in <strong>the</strong> fermentation<br />
process, <strong>the</strong> possibilities are that hydrophobic<br />
fl avor compounds are adsorbed onto<br />
<strong>the</strong> high hydrophobic cell surface and easily<br />
removed with yeasts from beer, and that will<br />
result in <strong>the</strong> light taste beer, while low CSH<br />
with low fl occulation characteristics will leave<br />
hop-derived hydrophobic compounds in<br />
beer with „Hefebittere“. Our results, using 5<br />
L scale fermentation, indicated that <strong>the</strong> concentrations<br />
of highly hydrophobic fl avor compounds,<br />
as myrcene, humulene, caryophyllene,<br />
farnecene, non-isomerized humulone<br />
analogue tend to be infl uenced by CSH degree<br />
and accumulate on <strong>the</strong> hydrophobic cell surface,<br />
and <strong>the</strong>n easily removed from beer.<br />
Toru Kishimoto<br />
Dr. Kishimoto got his PhD from Kyoto University<br />
in 2008, and received an Incentive<br />
Award in 2012 from <strong>the</strong> Brewing Society of<br />
Japan, <strong>for</strong> his research entitled Hop-Derived<br />
Odorants Contributing to <strong>the</strong> Aroma Characteristics<br />
of Beer. He graduated from <strong>the</strong> Graduate<br />
School of Agriculture, Kyoto University<br />
(Japan), and he joined <strong>the</strong> R&D Laboratory of<br />
Asahi Breweries Ltd. in 1999.<br />
54<br />
P 032<br />
The perfect beer taster?<br />
Eleanor K Woods 1<br />
1Campden BRI, Flavour and Innovation, Nutfi eld, United<br />
Kingdom<br />
DESCRIPTION OF TOPIC:<br />
Do men or women make better beer tasters?<br />
Existing studies from <strong>the</strong> food industry have<br />
suggested that women out-per<strong>for</strong>m men in<br />
a range of sensory methods - but does this<br />
hold true <strong>for</strong> beer tasting? In this study we<br />
have investigated diff erences in per<strong>for</strong>mance<br />
between male and female panellists in stimulus<br />
detection thresholds and in <strong>the</strong> ability to<br />
recognise and describe compounds from <strong>the</strong><br />
beer fl avour wheel. The study has included<br />
data from expert and untrained panellists to<br />
additionally highlight diff erences between<br />
how male and female tasters respond to training.<br />
The results from this study will allow<br />
us to predict whe<strong>the</strong>r gender balance in <strong>the</strong><br />
panel will infl uence <strong>the</strong> results of beer tasting<br />
in breweries.<br />
Eleanor K Woods<br />
After graduating from <strong>the</strong> University of Manchester<br />
with a BSc in Biology Eleanor joined<br />
<strong>the</strong> Sensory team at Campden BRI‘s Brewing<br />
Division. Since <strong>the</strong>n she has also been working<br />
with <strong>the</strong> University of Nottingham to<br />
achieve a Post-Graduate certifi cate in Sensory<br />
Science. Eleanor has worked with micro<br />
and multinational breweries alike to employ<br />
sensory methodologies to projects with aims<br />
such as QA/QC, benchmarking, taint identifi -<br />
cation and new product development.
P 033<br />
New hop technologies <strong>for</strong> special<br />
beer with unique fl avors<br />
Yoko Noro 1, Atsushi Murakami 1, Toru Gamo 2,<br />
Tomohiko Ichii 1<br />
1Kirin Company, Limited, Research & Development Division,<br />
Yokohama, Japan, 2Kirin <strong>Brewery</strong> Company, Limited, Marketing<br />
Department, Tokyo, Japan<br />
DESCRIPTION OF TOPIC:<br />
Kirin has succeeded in products with new hop<br />
technologies. We will introduce <strong>the</strong> technologies<br />
and <strong>the</strong>ir unique hop aroma components<br />
in beer.<br />
1. Non dried, freshly frozen hops: With frozen<br />
hops (frozen soon after picking), we developed<br />
an extremely unique beer fl avor. Frozen<br />
hops impart fresh and pure fl owery aroma.<br />
Content of linalool along with o<strong>the</strong>r terpene<br />
alcohols was high, and terpene oxides were<br />
low, possibly due to non-heat treatment.<br />
2. Dip Hopping: Dry-hopping emphasizes not<br />
only pleasant but also unpleasant hop aroma.<br />
To minimize <strong>the</strong> latter, we developed dip-hopping,<br />
which hops are added to a fermentation<br />
tank with yeast (diff er from dry-hopping). Dip<br />
hopped beer was high in linalool and low in<br />
myrcene compared to dry hopped beer. We<br />
assume that high linalool was due to low<br />
temperature extraction, and low myrcene was<br />
due to elimination by yeast. Also, low temperature<br />
prevented isomerization of alpha acids<br />
which enables control of hop aroma and bitterness<br />
individually.<br />
Yoko Noro<br />
Yoko Noro worked <strong>for</strong> Kirin <strong>Brewery</strong> Company<br />
Limited after receiving a master‘s degree<br />
in Agricultural science from Kyoto University<br />
in 2009. She worked in <strong>the</strong> Brewing Department<br />
of <strong>the</strong> Sendai brewery <strong>for</strong> three years<br />
as an assistant manager and <strong>the</strong>n entered<br />
<strong>the</strong> Brewing Technology Development Center<br />
w<strong>here</strong> she conducted research on hop aroma.<br />
From January 2013, owing to reorganization,<br />
she has been working <strong>for</strong> Research and Development<br />
Division in Kirin Company, Limited<br />
with <strong>the</strong> same hop <strong>the</strong>me.<br />
P 034<br />
Yeast biodiversity as a tool <strong>for</strong><br />
fl avor improvement in beer<br />
Catrienus de Jong 1, Amparo Gamero1,<br />
Michael Katz 2, Zoran Gojkovic 2<br />
1NIZO food research, Ede, The Ne<strong>the</strong>rlands, 2Carlsberg Group,<br />
Copenhagen, Denmark<br />
DESCRIPTION OF TOPIC:<br />
During <strong>the</strong> brewing process, Saccharomyces<br />
species contribute to beer aroma development,<br />
but hundreds of o<strong>the</strong>r poorly studied<br />
yeast species exist, being an underutilized resource<br />
with potential <strong>for</strong> product innovation.<br />
MicroBeer is a miniaturized brewing process<br />
(5 ml) highly useful <strong>for</strong> screening purposes.<br />
In this study, twelve strains presenting high<br />
potential <strong>for</strong> aroma <strong>for</strong>mation were tested<br />
in pure and mixed culture at 12°C. The results<br />
of <strong>the</strong> MicroBeer pointed out problems<br />
in maltose utilization and masked aromas in<br />
mixed cultures. These problems were solved<br />
in <strong>the</strong> lab-scale fermentations employing<br />
enzyme-treated wort and lower proportions<br />
of Saccharomyces yeast. This was a clear example<br />
of <strong>the</strong> usefulness of <strong>the</strong> microsystems<br />
to screen diff erent conditions as a tool <strong>for</strong><br />
improvement. In addition, some yeasts were<br />
able to produce high amount of esters, which<br />
could mean a potential increase in beer fruitiness<br />
and some yeast combinations result to<br />
be useful <strong>for</strong> fl avor improvement.<br />
Catrienus de Jong<br />
After fi nishing a study <strong>for</strong> analytical chemist,<br />
Catrienus de Jong was involved with <strong>the</strong> foundation<br />
of <strong>the</strong> fl avour research department at<br />
NIZO food research. Now 30 years later he<br />
is still active as senior scientist and project<br />
manager at NIZO food research and manages<br />
fl avour related research projects <strong>for</strong> customers<br />
all around <strong>the</strong> world. He is also responsible<br />
<strong>for</strong> many scientifi c publications and<br />
contributions at symposia.<br />
P 035<br />
Characterisation of fresh and<br />
aged single-hop beers via hop oil<br />
c onstituents fi ngerprinting and<br />
sensory assessment<br />
Filip Van Opstaele 1, , Ann Van Holle 2, Jan<br />
Van Nieuwenhove 2, Dirk Naudts 2, Denis De<br />
Keukeleire 3, Guido Aerts 1, Luc De Cooman 1<br />
1KAHO Sint-Lieven University College, KU Leuven Association,<br />
Ghent, Belgium, 2De ‚proef‘brouwerij, R&D department,<br />
Lochristi, Belgium, 3Em. Prof., Ghent University, Ghent,<br />
Belgium<br />
DESCRIPTION OF TOPIC:<br />
The focus of this study is on scrupulous investigation<br />
of hop oil constituent fi ngerprints of<br />
fresh and aged single-hop beers aromatised<br />
with particular hop varieties using late and<br />
dry hopping techniques. The results clearly<br />
refl ect analytical and sensorial diff erences<br />
between fresh late and dry-hopped beers<br />
produced with <strong>the</strong> same hop variety on <strong>the</strong><br />
one hand and between single-hop beers aromatised<br />
with diff erent varieties on <strong>the</strong> o<strong>the</strong>r<br />
hand. Upon ageing a signifi cant decrease of<br />
<strong>the</strong> level of hop oil esters and terpene hydrocarbons<br />
was observed <strong>for</strong> all beers. Our<br />
results point to adsorption of volatiles at <strong>the</strong><br />
haze <strong>for</strong>med upon ageing and adsorption of<br />
particular volatiles at <strong>the</strong> crown liners. Hop oil<br />
volatiles belonging to <strong>the</strong> ester and monoterpene<br />
hydrocarbons group always decrease to<br />
a higher extent in dry-hopped beers. Sensorial<br />
data show that <strong>the</strong> fresh fl avour of dry hopped<br />
beers is less stable compared with <strong>the</strong><br />
corresponding late hopped beers investigated<br />
in this study.<br />
Filip Van Opstaele<br />
Filip Van Opstaele obtained an academic degree<br />
in Industrial Engineering in Biochemistry<br />
from KAHO Sint-Lieven (Gent, Belgium) in<br />
1993 and started working at KAHO as scientifi<br />
c researcher. In 2001 he joined <strong>the</strong> Biochemistry<br />
Department as assistant scientist at<br />
EFBT (KAHO Sint-Lieven). In 2011 he obtained<br />
<strong>the</strong> degree of Doctor in Bioscience Engineering.<br />
His research experience is in <strong>the</strong> fi eld of<br />
fl avour (bio)chemistry, hopping technology,<br />
and (hyphenated) mass spectrometric techniques.<br />
55 34 TH EBC CONGRESS · LUXEMBOURG 26 – 30 MAY 2013
P 036<br />
From wort to beer: The evolution<br />
of hoppy aroma of single hopped<br />
beers produced by early kettle<br />
hopping, late kettle hopping and dry<br />
hopping<br />
Michael Dresel 1, Tatiana Praet 1, Filip<br />
Van Opstaele 1, Ann Van Holle 2, Jan Van<br />
Nieuwenhove 2, Dirk Naudts 2, Denis De<br />
Keukeleire 3, Guido Aerts 1, Luc De Cooman 1<br />
1KAHO Sint-Lieven University College, KU Leuven Association,<br />
Laboratory of Enzyme, Fermentation and Brewing<br />
Technology, Ghent, Belgium, 2De ‚proef‘brouwerij, R&D<br />
Department, Lochristi, Belgium, 3Em. Prof., Ghent University,<br />
Ghent, Belgium<br />
DESCRIPTION OF TOPIC:<br />
Knowledge of <strong>the</strong> impact of early kettle, late<br />
kettle and dry hopping on <strong>the</strong> fi nal fl avour<br />
sensation of beers is ra<strong>the</strong>r fragmentary. To<br />
gain insights into <strong>the</strong> way hoppy aroma develops,<br />
samples were taken at diff erent stages<br />
along <strong>the</strong> brewing process of single-hopped<br />
beers and analysed, t<strong>here</strong>by focussing on accurate<br />
determination of <strong>the</strong> full spectrum of<br />
hop oil-derived components. This study pinpoints<br />
analytical and sensory changes induced<br />
by <strong>the</strong> boiling and fermentation process,<br />
lagering, beer clarifi cation and pasteurisation.<br />
Our data shows both qualitative and quantitative<br />
changes of hop oil-derived volatiles at<br />
<strong>the</strong> <strong>for</strong>mer mentioned production stages and<br />
reveals that individual analytes greatly diff er<br />
in <strong>the</strong>ir behaviour, e.g. clarifi cation caused a<br />
crucial decrease of volatiles associated with<br />
<strong>the</strong> so-called ‚noble/spicy‘ aroma. Fur<strong>the</strong>rmore,<br />
<strong>the</strong> choice of <strong>the</strong> hop variety used <strong>for</strong><br />
dry hopping, contributes signifi cantly to <strong>the</strong><br />
overall perceivable aroma by boosting individual<br />
volatiles.<br />
Michael Dresel<br />
Michael Dresel studied Food Chemistry (2003-<br />
2007) and became „State Certifi ed Food Chemist“<br />
after 1 year at <strong>the</strong> bavarian state (2008).<br />
2009-2012, he worked on his PhD <strong>the</strong>sis on<br />
hops hard resin (T. Hofmann, TU Munich) and<br />
completed a 2 month exchange program<br />
under <strong>the</strong> supervision of J.F. Stevens (Linus<br />
Pauling Institute, Oregon State University) in<br />
2012. Since Nov. 2012 he works in Belgium as<br />
a Post-Doc at KAHO Sint-Lieven. His research<br />
focuses on sensory-active compounds of<br />
hops and beer.<br />
P 037<br />
A simple concept to predict beer<br />
aging by use of statistical tools<br />
Annika Lagemann 1, Betina Kessler 2,<br />
Waltraud Kessler 3, Georg Stettner 1<br />
1Bitburger Braugruppe GmbH, Bitburg, Germany, 2Technische<br />
Universität München, Center of Life Sciences Weihenstephan,<br />
Freising, Germany, 3Reutlingen-University, Faculty of Applied<br />
Chemistry, Reutlingen, Germany<br />
DESCRIPTION OF TOPIC:<br />
T<strong>here</strong> are numerous volatile fl avoring substances<br />
whose infl uence on <strong>the</strong> fl avor of<br />
aged beer is widely discussed in literature.<br />
For practical reasons, <strong>the</strong> complete analysis<br />
of all of those compounds could not be incorporated<br />
into everyday laboratory routines. For<br />
this reason, we made a selection of 37 volatile<br />
compounds which were measured in fresh<br />
Pils and beer that had been stored <strong>for</strong> six<br />
months via GC-MS/MS. Measurements were<br />
carried out monthly over a time course of two<br />
years. By use of <strong>the</strong> principal component analysis<br />
on this dataset, we were able to identify<br />
nine key substances which allow a classifi -<br />
cation as ei<strong>the</strong>r fresh or stored beer, with an<br />
accuracy of 90%. The validity of this data was<br />
confi rmed by sensory recombination experiments.<br />
Due to its reliability and <strong>the</strong> largely automated<br />
instrumental detection, this simple<br />
tool marks a noticeable facilitation regarding<br />
prediction capabilities of beer aging, which<br />
o<strong>the</strong>rwise would only be possible by means<br />
of human sensory analysis.<br />
Annika Lagemann<br />
Laboratory manager, Bitburger Braugruppe<br />
GmbH, Bitburg, Germany (July 2011 to present)<br />
Flavour scientist, Bitburger Braugruppe<br />
GmbH, Bitburg, Germany (September 2008 to<br />
June 2011) Scientifi c assistant, Chair of Food<br />
Chemistry and Molecular Sensory Science,<br />
TUM, Freising, Germany (Oktober 2007 to July<br />
2008) Scientifi c assistant, Institute of Food<br />
Chemistry, WWU, Münster, Germany (May<br />
2005 to September 2007) Study of Food Chemistry,<br />
University of Bonn and CVUA Münster<br />
(1998-2005)<br />
56<br />
P 038<br />
Diff erent infl uences on generation<br />
of aging compounds and <strong>the</strong> improvement<br />
of <strong>the</strong> oxidative beer fl avour<br />
stability using active packing material<br />
versus SO 2-additon<br />
Constanze Ruff 1, Thomas Kunz 1,<br />
Frank-Jürgen Methner 1<br />
1Technische Universität Berlin, Lab of Brewing Science,<br />
Berlin, Germany<br />
DESCRIPTION OF TOPIC:<br />
Beer off fl avour caused by oxidation has<br />
been attributed to <strong>the</strong> <strong>for</strong>mation of specifi<br />
c aging compounds. The mechanisms may<br />
diff er but <strong>the</strong>y all involve activated oxygen<br />
species, highlighting <strong>the</strong> importance to avoid<br />
oxygen entry during <strong>the</strong> brewing process<br />
and storage. The technique combination of<br />
EPR-spectroscopy and <strong>the</strong> ascertain of specifi<br />
c aging compounds as oxygen indicator via<br />
GC-MS is predestined to get a deeper inside in<br />
infl uences of SO 2-additon in comparison and<br />
in combination to diff erent crown liners with<br />
O 2-scavenger properties on <strong>the</strong> oxidative beer<br />
stability. As expected beer with SO 2-additon<br />
and diff erent O 2-scavenger exhibited higher<br />
fl avour stability. A remarkable and <strong>for</strong> <strong>the</strong><br />
mechanism important point is that a comparable<br />
increase in oxidative stability with SO 2additon<br />
or O 2-scavenger leads to a diff erent<br />
positive eff ect on <strong>the</strong> <strong>for</strong>mation of aging compounds<br />
during storage. The results off er a<br />
useful knowledge about <strong>the</strong> right proceeding<br />
to increase oxidative beer stability.<br />
Constanze Ruff<br />
Constanze Ruff has started her scientifi c career<br />
with having a chemistry summer school<br />
at <strong>the</strong> FU Berlin. After fi nishing school she joined<br />
an internship at Herbstreith & Fox Company.<br />
She started studying chemistry at <strong>the</strong><br />
Freie Universität Berlin. In March 2011 she<br />
switched to <strong>the</strong> TU Berlin to <strong>the</strong> compartement<br />
of Food Chemistry. At <strong>the</strong> moment she<br />
is working at her pre-Diploma. Her research<br />
work began in Nov. 2010 as a student research<br />
assistant at <strong>the</strong> TU Berlin Lab of Brewing<br />
Science.
P 039<br />
Re-inventing <strong>the</strong> wheel:<br />
sensory evaluation and <strong>the</strong> key to<br />
drinkability<br />
Alex G Barlow 1, Rob S T Lin<strong>for</strong>th 2, Haobin<br />
Wang 2, David Cook 2<br />
1ALL BEER, Sheffi eld, United Kingdom, 2University of<br />
Nottingham, Nottingham, United Kingdom<br />
DESCRIPTION OF TOPIC:<br />
Organoleptic evaluation of beer fl avour is key<br />
to acceptance and popularity amongst consumers.<br />
Whilst researching <strong>the</strong> ALL beer guide<br />
book, <strong>the</strong> author re-invented Mailgaard‘s fl avour<br />
wheel descriptors in <strong>the</strong> <strong>for</strong>mat of a balance<br />
containing sensory characteristics profi<br />
ling aroma, taste and mouthfeel. This ALL<br />
Beer Flavour Notepad (ABFN) was used to<br />
evaluate <strong>the</strong> fl avours of 550 beers spanning<br />
a broad range of families and styles. Over 50<br />
data points were characterised <strong>for</strong> each beer<br />
and consolidated into a database to analyse<br />
and search beers by fl avour characteristics.<br />
Statistical analysis, including PCO, succeeded<br />
in confi rming expected beer family and style<br />
characteristics and <strong>the</strong> ABFN as a method of<br />
compiling fl avour data. ABFN conferred <strong>the</strong><br />
ability to empirically measure beer balance<br />
and fl avour strength (intensity). These factors<br />
toge<strong>the</strong>r with personal preference are key to<br />
beer drinkability, so ABFN links sensory evaluation<br />
with smart methods of fl avour communication<br />
to consumers.<br />
Alex G Barlow<br />
Alex‘s passion <strong>for</strong> beer started with his fi rst<br />
job, as cellar-man at his local pub. With over<br />
25 years international brewing and retail<br />
trade experience, he‘s a qualifi ed Master<br />
Brewer, experienced brewing consultant and<br />
presenter, cicerone and frequent beer judge.<br />
He is author of <strong>the</strong> award winning ALL BEER<br />
Guide and is committed to enhancing peoples<br />
enjoyment of beer through eff ective communication:<br />
Flavour > Discovery > Choice.<br />
P 039a<br />
Flavour and hop aroma stability of<br />
craft beers packed in diff erent PET<br />
bottles<br />
Roland Folz 1, Christina Schönberger 2, Andreas<br />
Gahr 3, Roland Pahl 1, Ruslan Hofmann 1<br />
1Versuchs- und Lehranstalt für Brauerei in Berlin (VLB) e.V.,<br />
Berlin, Germany, 2Barth Innovations, Nuremberg, Germany,<br />
3Hopfenveredlung St. Johann, Train-St. Johann, Germany<br />
DESCRIPTION OF TOPIC:<br />
Craft beers with hop intense fl avours are tremendously<br />
successful currently in various<br />
markets. The fl avour stability characteristics<br />
of Craft beers are scarcely investigated but<br />
will become important with nationwide or international<br />
distribution. PET bottles are due<br />
to <strong>the</strong>ir low weight convenient <strong>for</strong> consumers<br />
and longer transport. The market share <strong>for</strong><br />
PET bottles shows steady growth and with<br />
increasing success of Craft beers this way of<br />
bottling may gain more importance. Two typical<br />
Craft beer types, a Pilsner and an India<br />
Pale Ale, were fi lled in PET bottles with diff erent<br />
barrier properties (Monolayer PET w and<br />
w/o coating). Bottles were stored at 4°C and<br />
23°C to study <strong>the</strong> infl uence of temperature<br />
and barrier per<strong>for</strong>mance on fl avour stability.<br />
It was shown that both factors infl uence <strong>the</strong><br />
fl avour stability and <strong>the</strong> development of typical<br />
stale fl avours. Fur<strong>the</strong>rmore, <strong>the</strong> India Pale<br />
Ale proved to be more stable in sensory context<br />
than <strong>the</strong> Pilsner, independent from <strong>the</strong><br />
PET material used.<br />
Roland Folz<br />
In his function as Head of <strong>the</strong> VLB Department<br />
Brewing & Beverage Science and Applications<br />
(BBSA) Dr. Roland Folz is involved in driving<br />
<strong>for</strong>ward <strong>the</strong> process of <strong>the</strong> VLB`s internationalization.<br />
The VLB is a German independent<br />
research institute and service provider <strong>for</strong><br />
<strong>the</strong> brewing and beverage industry. Under<br />
Roland‘s leadership <strong>the</strong> BBSA has become an<br />
international standing provider <strong>for</strong> research<br />
and solutions regarding technological topics,<br />
global consultancies and international training<br />
courses.<br />
P 040<br />
Microbiology of lambic sour beers<br />
processing unraveled with Matrix-<br />
Assisted Laser Desorption/Ionisation<br />
Time-of-Flight Mass Spectrometry<br />
(MALDI-TOF MS)<br />
Freek Spitaels 1, , Maarten Janssens 2,<br />
Anita Van Landschoot 3, Luc De Vuyst 2,<br />
Peter Vandamme 1<br />
1Ghent University, Department of Biochemistry and Microbiology,<br />
Laboratory of Microbiology (LM-UGent), Ghent, Belgium,<br />
2Vrije Universiteit Brussel, Department of Bio-engineering<br />
Sciences, Research Group of Industrial Microbiology and<br />
Food Biotechnology (IMDO), Elsene, Belgium, 3University<br />
College Ghent, Faculty Applied Bioscience Engineering,<br />
Laboratory of Biochemistry and Brewing, Ghent, Belgium<br />
DESCRIPTION OF TOPIC:<br />
Lambic sour beers are <strong>the</strong> products of spontaneous<br />
fermentation and maturation in<br />
casks that progresses <strong>for</strong> at least two years<br />
and which is hardly characterized from a microbiological<br />
point of view. The present study<br />
aimed to evaluate <strong>the</strong> quality of <strong>the</strong>se beers<br />
by identifying <strong>the</strong> very complex microbiota<br />
using mass spectrometry. First, a MALDI-TOF<br />
mass spectra database was built using 4000<br />
bacterial and yeast isolates from several<br />
batches and stages of lambic fermentations.<br />
This database also included mass spectra<br />
of additional reference strains of <strong>the</strong> same<br />
species, but isolated from o<strong>the</strong>r sources, to<br />
account <strong>for</strong> biological variation. Currently, <strong>the</strong><br />
database includes isolates representing 600<br />
species from 150 genera, <strong>for</strong> instance all species<br />
of <strong>the</strong> genera Pediococcus, Acetobacter<br />
and Gluconobacter. The database is now used<br />
not only <strong>for</strong> <strong>the</strong> identifi cation of novel isolates,<br />
but also <strong>for</strong> direct identifi cation of microorganisms<br />
in samples with a complex microbiota.<br />
Freek Spitaels<br />
Freek Spitaels obtained his degree as Master<br />
in Biochemistry and Biotechnology (major<br />
microbiology) at Ghent University (Belgium)<br />
in 2009. The same year, he started working<br />
as PhD student at <strong>the</strong> Laboratory of Microbiology<br />
(Ghent University). His study focuses<br />
on <strong>the</strong> microbiological composition in <strong>the</strong><br />
various stages of lambic sour beers fermentation,<br />
using primarily Matrix-Assisted Laser<br />
Desorption/Ionisation Time-Of-Flight Mass<br />
Spectrometry (MALDI-TOF MS).<br />
57 34 TH EBC CONGRESS · LUXEMBOURG 26 – 30 MAY 2013
P 041<br />
Exploration of matrix-assisted laser<br />
desorption/ionization-time of fl ight<br />
mass spectrometry (MALDI-TOF<br />
MS) as a fast identifi cation tool <strong>for</strong><br />
beer spoilage bacteria<br />
Anneleen D. Wieme 1, Anita Van Landschoot 1,<br />
Peter Vandamme 2<br />
1University College Ghent, Faculty of Applied Bioscience<br />
Engineering, Laboratory of Biochemistry and Brewing, Ghent,<br />
Belgium, 2Ghent University, Department of Biochemistry and<br />
Microbiology, Laboratory of Microbiology, Ghent, Belgium<br />
DESCRIPTION OF TOPIC:<br />
Beer spoilage induced by bacteria is a common<br />
problem in <strong>the</strong> brewing industry and has<br />
a great impact on <strong>the</strong> brewing economy. The<br />
present study aims to develop a quick, accurate<br />
and inexpensive method to detect and<br />
identify beer spoilage bacteria. To achieve<br />
this, an extensive database comprising about<br />
6500 MALDI-TOF MS-profi les including more<br />
than 260 accurately identifi ed contaminants<br />
and beer spoilage isolates was built. The 260<br />
isolates represent all commonly encountered<br />
spoilage bacteria with a focus on lactobacilli,<br />
acetic acid bacteria and some anaerobes.<br />
The profi les revealed culture-independent<br />
species-specifi c biomarker peaks <strong>for</strong> all spoilage<br />
species, allowing straight<strong>for</strong>ward identifi<br />
cation of novel isolates. The fi nal aim of <strong>the</strong><br />
present study is to detect and identify spoilage<br />
bacteria in a sample with no or minimal<br />
culture steps.<br />
Anneleen D. Wieme<br />
Anneleen Wieme graduated in 2009 as a Master<br />
in Industrial Sciences Biochemistry and<br />
Biotechnology at <strong>the</strong> University College Ghent.<br />
Currently, she is working at <strong>the</strong> lab of Biochemistry<br />
and Brewing at <strong>the</strong> University College<br />
Ghent and is obtaining her Ph.D. (3rd year), in<br />
association with Ghent University, at <strong>the</strong> lab<br />
of Microbiology at <strong>the</strong> Faculty of Sciences.<br />
The results of her study will help <strong>the</strong> brewing<br />
industry in quickly identifying and controlling<br />
bacterial beer spoilage using MALDI-TOF MS.<br />
P 042<br />
Molecular characterisation of lactic<br />
acid bacteria community during<br />
malting<br />
Sofi e Malfl iet 1, , Annelies Justé 2, Bart<br />
Lievens 2, Luc De Cooman 1, Guido Aerts 1<br />
1KAHO St.-Lieven, Laboratory of Enzyme, Fermentation, and<br />
Brewing Technology (EFBT), Department Chemistry/Biochemistry<br />
- LFoRCe, Department M2S, KU Leuven, Gent, Belgium,<br />
2Lessius Hogeschool, Laboratory <strong>for</strong> Process Microbial Ecology<br />
and Bioinspirational Management (MPE&BIM) - LFoRCe,<br />
Department M2S, KU Leuven, Sint-Katelijne-Waver, Belgium<br />
DESCRIPTION OF TOPIC:<br />
Knowledge of microbial dynamics during<br />
malting is limited, partly because conventional<br />
culture-dependent approaches result in<br />
an incomplete picture of <strong>the</strong> microbial diversity.<br />
In particular, not much is known about<br />
<strong>the</strong> structure and dynamics of <strong>the</strong> lactic acid<br />
bacteria (LAB) community, yet one of <strong>the</strong> most<br />
important bacterial groups in malting. In this<br />
study, <strong>the</strong> LAB microbial community structure<br />
was assessed <strong>for</strong> malting samples from different<br />
industrial malting systems, i.e. from<br />
barley to malt using two culture-independent<br />
techniques, including T-RFLP and 454 pyrosequencing.<br />
Using T-RFLP, <strong>the</strong> LAB community<br />
seemed remarkably stable during malting,<br />
with <strong>the</strong> most predominant LABs originating<br />
from <strong>the</strong> barley itself. Conversely, <strong>the</strong> sensitive<br />
454 pyrosequencing revealed diff erences<br />
between LAB community structures during<br />
malting, with predominance of Weissella species.<br />
Our results create a scientifi c basis <strong>for</strong><br />
selection of specifi c LABs in view of process<br />
management in industrial malting.<br />
Sofi e Malfl iet<br />
In 2004, Sofi e Malfl iet obtained her MSc in<br />
Industrial Sciences in Biochemistry at KAHO<br />
St.-Lieven in Ghent, Belgium. After graduation,<br />
she started working as a Research Assistant<br />
at <strong>the</strong> Laboratory of Enzyme, Fermentation<br />
and Brewing Technology of KAHO St.-Lieven<br />
on fl avour quality and fl avour stability of pilsner<br />
and light beers. She is currently fi nishing<br />
her PhD on microfl ora management during<br />
malting, in particular on xylanase-producing<br />
bacteria and <strong>the</strong>ir infl uence on wort fi ltration<br />
rate.<br />
58<br />
P 043<br />
Application of a new biological<br />
detoxifying method in malting<br />
Pedro Oliveira 1, Fritz Jacob 2, Emanuele<br />
Zannini 1, Elke Arendt 1<br />
1University College Cork, Cork, Ireland, 2Forschungszentrum<br />
Weihenstephan für Brau- und Lebensmittelqualität, Technische<br />
Universität München, Freising, Germany<br />
DESCRIPTION OF TOPIC:<br />
Lactic acid Bacteria (LAB) have long been<br />
known to have <strong>the</strong> ability to delay fungal<br />
growth in food by producing antifungal compounds,<br />
and barley crops infected with Fusarium<br />
moulds have been a continuing problem<br />
<strong>for</strong> <strong>the</strong> malting and brewing industries.The<br />
objective of this project was to develop a biological<br />
method <strong>for</strong> early fungal detoxifi cation<br />
with application in malting using a brewing<br />
wort substrate. Fusarium culmorum was<br />
challenged gainst 5 specifi c LAB strains with<br />
positive antifungal activity. The method was<br />
validated comparing acidifi ed medium and<br />
negative antifungal LAB strain´s as controls.<br />
The antifungal potential screening using a<br />
microplate assay showed a complete inhibition<br />
of fungi <strong>for</strong> 7 days. The best antifungal<br />
LAB strains were tested in a micro-malting<br />
trial and quantifi ed <strong>for</strong> fungal growth (PCR)<br />
and mycotoxins (HPLC). An up-scale to 1.5 kg<br />
batch was carried to evaluate <strong>the</strong> malt quality.<br />
The Application of <strong>the</strong> new biological detoxifying<br />
method was successfull.<br />
Pedro Oliveira<br />
Pedro Oliveira studied Food Science and<br />
Engineering at Technical University of Lisbon.<br />
Pedro was awarded with a mobilization<br />
scholarship <strong>for</strong> one year to study at University<br />
College Cork w<strong>here</strong> he completed <strong>the</strong> fi -<br />
nal graduation project on NPD and Beverage<br />
Science. In 2010, Pedro Oliveira was awarded<br />
with a Postgraduate Scholarship and he joined<br />
Prof. Arendt research team at <strong>the</strong> University<br />
College Cork <strong>for</strong> his PhD Project on <strong>the</strong><br />
identifi cation of antifungal compounds from<br />
LAB.
P 044<br />
Fast and reliable detection of beer<br />
spoilage bacteria <strong>for</strong> routine analysis-<br />
fi eld results<br />
Jürgen Günter Ziehl 1, Georg Stettner 2, Horst<br />
Born 3, Caroline Knoll 3<br />
1Pall GmbH, Sales & Marketing, Bad Kreuznach, Germany,<br />
2Bitburger Braugruppe GmbH, TQ/Technologie und Qualitätswesen,<br />
Bitburg, Germany, 3Pall GmbH, SLS Global Technical<br />
Support, Bad Kreuznach, Germany<br />
DESCRIPTION OF TOPIC:<br />
Microbiological beer stability is considered to<br />
be one of <strong>the</strong> most critical control parameters<br />
<strong>for</strong> beer quality and <strong>the</strong> brewery‘s brand protection.<br />
The potential presence and growth of<br />
beer-spoilage bacteria in <strong>the</strong> fi nal product is<br />
<strong>the</strong> main reason <strong>for</strong> causing changes in fl avor,<br />
taste and turbidity. A critical challenge of all<br />
conventional microbiological test methods is<br />
<strong>the</strong> timely release of <strong>the</strong> analyzed products<br />
which typically takes 6-7 days until fi nal result.<br />
To identify potential risks at an early stage<br />
and to initiate counteractive measures, a<br />
precise and rapid identifi cation of <strong>the</strong> relevant<br />
organisms is essential. This study shows <strong>the</strong><br />
pros and cons of <strong>the</strong> conventional microbiological<br />
test methods and a comparison of <strong>the</strong><br />
current in use Polymerase Chain Reaction<br />
technology with <strong>the</strong> new Pall GeneDisc® PCR<br />
System and its beer specifi c beer plate. Both<br />
systems have been tested on <strong>the</strong>ir handling,<br />
reliability and sensibility, <strong>the</strong> most important<br />
key parameters on daily routine analysis.<br />
Jürgen Günter Ziehl<br />
Three years apprenticeship as Brewer&Malter<br />
at Parbrauerei in Primasens, Germany. Study<br />
at TU Berlin with degree as Dipl. Brewmaster.<br />
From 1993 to 2000 technical sales of silica<br />
gels at Stabifi x Brauerei Technik, Munich.<br />
From 1999 to 2002 postgraduate studies in<br />
economics at <strong>the</strong> AKAD, Lahn, degree as Industrial<br />
Engineer. From 2000 on responsible<br />
<strong>for</strong> PVPP business in <strong>the</strong> beverage industry<br />
<strong>for</strong> BASF Ludwigshafen, Germany. Since 2003<br />
with Pall, at present as Global Business Deveolpement<br />
Manager Beer.<br />
P 045<br />
A novel tool to establish volatile<br />
molecular biomarkers to evaluate<br />
yeasts per<strong>for</strong>mance through beer<br />
fermentation: S. cerevisiae and S.<br />
pastorianus<br />
Cátia Martins 1, Adelaide Almeida 2, Tiago<br />
Brandão 3, Sílvia Rocha 1<br />
1University of Aveiro, Chemistry Department, QOPNA, Aveiro,<br />
Portugal, 2University of Aveiro, Biology Department, CESAM,<br />
Aveiro, Portugal, 3Unicer Bebidas, SA, Leça do Balio, Portugal<br />
DESCRIPTION OF TOPIC:<br />
Yeasts are one main intrinsic factor on <strong>the</strong><br />
quality of beer due to <strong>the</strong>ir impact on fl avour.<br />
Volatile metabolites produced by yeasts play<br />
a crucial role on beer aroma peculiarities.<br />
The aim of this research was to use a highthroughput<br />
methodology <strong>for</strong> comprehensive<br />
and in-depth analysis of S. cerevisiae and S.<br />
pastorianus volatile exo-metabolome using<br />
solid phase microextraction combined with<br />
comprehensive two-dimensional gas chromatography-time-of-fl<br />
ight mass spectrometry,<br />
in order to evaluate <strong>the</strong> yeast per<strong>for</strong>mance<br />
through beer fermentation.<br />
This methodology allowed <strong>the</strong> detection of<br />
around 1000 metabolites <strong>for</strong> both strains, distributed<br />
over several chemical groups: acids,<br />
aldehydes, alcohols, esters, terpenoids, ketones,<br />
sulphur compounds. This approach can<br />
be used as a tool to establish molecular biomarkers<br />
of yeast per<strong>for</strong>mance through beer<br />
fermentation.<br />
We thank FCT <strong>for</strong> fi nancial support of Research<br />
Unit 62/94-QOPNA (project PEst-C/<br />
QUI/UI0062/2011) and PhD grant SFRH/<br />
BD/77988/2011.<br />
Cátia Martins<br />
I have a Food Biotechnology master‘s degree<br />
at University of Aveiro. Currently, I‘m a PhD<br />
student at University of Aveiro, w<strong>here</strong> <strong>the</strong> <strong>the</strong>me<br />
of my <strong>the</strong>sis is correlated with <strong>the</strong> reuse<br />
of yeast on <strong>the</strong> brewing process<br />
P 046<br />
Preventing <strong>the</strong> quality of high gravity<br />
beer from lactic contamination<br />
with lysozyme <strong>for</strong>mulation<br />
Gilles Goemaere 1, Dorothée Maurel 2, Walter<br />
Mulinazzi 2, Philippe Cario 1<br />
1SPINDAL AEB Group, Beverage Division, Gretz-Armainvilliers,<br />
France, 2SPINDAL AEB Group, Research & Development<br />
Biotechnology, Gretz-Armainvilliers, France<br />
DESCRIPTION OF TOPIC:<br />
Lysozyme is described as an enzyme approved<br />
in <strong>the</strong> agro alimentary industry as a treating<br />
material to stabilize beverage from malolactic<br />
acid bacterial degradation. The aim<br />
of this paper is to present a number of trials<br />
which have been conducted on high gravity<br />
beer in order to check <strong>the</strong> action of lysozyme<br />
<strong>for</strong>mulation on lactic contamination.<br />
The amount of lactic bacteria on an infected<br />
beer was tested on a MRS media without oxygen<br />
intake. Diff erent dosages were applied,<br />
from 0,2 up to 5 g/hL and compared with reference.<br />
Results and comments after 6 weeks<br />
of incubation were achieved on beer followed<br />
by <strong>the</strong> analysis of a number of aldehydes. A<br />
<strong>for</strong>cing test was also realized to measure <strong>the</strong><br />
impact on sensible protein and <strong>the</strong> general<br />
haze stability.<br />
Analysis of beer treated with this enzyme revealed<br />
that it does not alter <strong>the</strong> characteristics<br />
of <strong>the</strong> fi nal beer; organoleptic degustation<br />
indicated that t<strong>here</strong> was a slight diff erence in<br />
taste in favor of <strong>the</strong> treated samples.<br />
Gilles Goemaere<br />
Brewing Engineer from <strong>the</strong> University of Louvain<br />
La Neuve, Gilles Goemaere fi rst started<br />
brewing in a Belgium facility until he decided<br />
to join AEB Group in 2011. Gilles has been<br />
following <strong>the</strong> French and Belgium breweries<br />
in order to optimize <strong>the</strong>ir process mainly in<br />
<strong>the</strong> fi eld of clarifi cation and fl avor stability. He<br />
has been developping a number of enzymatic<br />
<strong>for</strong>mulation to improve quality and yield in <strong>the</strong><br />
brehouse.<br />
59 34 TH EBC CONGRESS · LUXEMBOURG 26 – 30 MAY 2013
P 047<br />
Consumer preference <strong>for</strong> sensory<br />
characteristics of commercial<br />
beers. Liking, disliking and appropriateness<br />
to diff erent usage situations<br />
Gianluca Donadini 1, Maria Daria Fumi 1<br />
1Università Cattolica del Sacro Cuore, Institute of Oenology<br />
and Food Engineering, Piacenza, Italy<br />
DESCRIPTION OF TOPIC:<br />
Understanding which sensory attributes drive<br />
consumer acceptance of beer is critical to <strong>the</strong><br />
Brewing Industry. Likewise it is critical to understand<br />
how a given market segments in<br />
terms of preference <strong>for</strong> sensory qualities of<br />
beer. A sensory profi ling and a consumer test<br />
including 18 commercially available beers<br />
of diff erent styles were conducted. A trained<br />
panel (n=8) completed <strong>the</strong> sensory profi ling<br />
using 35 descriptors. 156 regular beer consumers<br />
tasted <strong>the</strong> same 18 beers in a hedonic<br />
test (aff ective response) and in an appropriateness<br />
test (cognitive-contextual response).<br />
The hedonic response of consumers was<br />
heterogeneous. Drivers of liking varied much<br />
across consumers who shift in sensory oriented<br />
consumers and in consumers who don‘t<br />
respond to sensory features of beer. Appropriateness<br />
<strong>for</strong> diff erent contexts and usage<br />
situations depended on liking mainly but consumers<br />
consider specifi c fl avours as more<br />
appropriate than o<strong>the</strong>rs to fi t a given context<br />
or usage situation.<br />
Gianluca Donadini<br />
Biologist. He‘s published studies on his research<br />
on fl avour profi ling of foods, consumer<br />
science & food security in refereed journal<br />
articles, national and international congress<br />
proceedings and numerous technical publications.<br />
They included <strong>the</strong> Journal of <strong>the</strong> Institute<br />
of Brewing, Food Research International,<br />
LWT Food Science & Technology, Food Control,<br />
Journal of Food Composition and Analysis,<br />
Food Additives and Contaminants, Journal of<br />
Sensory Studies, <strong>European</strong> Food Research<br />
and Technology.<br />
P 048<br />
A kinetic study of <strong>the</strong> <strong>for</strong>mation of<br />
2- and 3-Methylbutanal<br />
Yarong Huang 1, Johannes Tippmann 1,<br />
Thomas Becker 1<br />
1Technische Universität München, Lehrstuhl für Brau- und<br />
Getränketechnologie, Freising, Germany<br />
DESCRIPTION OF TOPIC:<br />
The development of a kinetic modeling of<br />
<strong>the</strong> <strong>for</strong>mation of 2- and 3-MB is a useful<br />
tool to estimate <strong>the</strong> wort fl avor during <strong>the</strong><br />
wort boiling and <strong>the</strong> fl avor stability of beer.<br />
A model experiment was per<strong>for</strong>med, using<br />
D-glucose/D-maltose and L-leucin/isoleucin<br />
to study Maillard reaction in a buff er solution<br />
of a pH value of 5.2. The development of<br />
this kinetic model was based on <strong>the</strong> correlation<br />
between <strong>the</strong> concentrations of 2-, 3-MB<br />
and <strong>the</strong> concentration of L-leucin/isoleucin.<br />
According to a statistical analysis, t<strong>here</strong> is no<br />
signifi cant diff erence in changes on concentration<br />
of D-Glucose/D-Maltose. In order to<br />
understand <strong>the</strong> reaction mechanism better, a<br />
multi response kinetic model was used. Using<br />
<strong>the</strong> software A<strong>the</strong>na Visual StudioTM, <strong>the</strong> reaction<br />
rates of <strong>the</strong> main reaction step of <strong>the</strong><br />
Maillard reaction and <strong>the</strong> activation energy<br />
were calculated respectively. The developed<br />
kinetic model gives a good fi t with <strong>the</strong> experimental<br />
data.<br />
Yarong Huang<br />
She studied Brewing Science and Beverage<br />
Technology and graduated with a Dipl.-Eng.<br />
Degree in brewing and beverage technology<br />
from <strong>the</strong> Technical University Munich (TUM) in<br />
<strong>the</strong> year 2009. Since December 2009 she has<br />
been employed as a PhD student in <strong>the</strong> fi eld<br />
of brewing research to investigate <strong>the</strong> chemical<br />
reactions of important process markers<br />
during wort boiling processes at <strong>the</strong> TUM in<br />
Freising-Weihenstephan, chair of Brewing<br />
and Beverage Technology (Prof. Dr. Thomas<br />
Becker).<br />
60<br />
P 049<br />
Origin of <strong>the</strong> fl avour active aldehydes<br />
cis- and trans-4,5-Epoxy-2Edecenal<br />
Konrad Neumann 1, Leif A. Garbe 1<br />
1VLB Berlin / TU Berlin, Berlin, Germany<br />
DESCRIPTION OF TOPIC:<br />
Flavor active cis- and trans-4,5-epoxy-2E-decenal<br />
isomers are important (off )-fl avor compounds<br />
in foods. Trans-4,5-epoxy-2E-decenal<br />
has a very intensive metallic taste and smell<br />
with very low thresholds. In <strong>the</strong> literature,<br />
<strong>the</strong> aroma of cis-4,5-epoxy-2E-decenal is described<br />
as citrus-like, sweet, fatty and malty.<br />
Even <strong>the</strong> concentrations of epoxydecenales<br />
are comparably low, epoxydecenal isomers<br />
were identifi ed as key odorants of food products.<br />
In fresh beer we analyzed epoxydecenal<br />
concentrations with 20pg/L. As <strong>the</strong>ir<br />
origin, degradation of linoleic acid is proposed<br />
but not yet verifi ed. To prove origination, we<br />
added stable isotope labeled linoleic acid to<br />
beer. In a second set-up we used 18O in <strong>the</strong><br />
head space of <strong>the</strong> beer bottles. By previously<br />
described workup procedures, and <strong>the</strong> usage<br />
of GC-MS-Methods we could trace <strong>the</strong> isotope<br />
labels appearing in <strong>the</strong> degradation products<br />
of linoleic acid and so elucidate <strong>the</strong> origin of<br />
both isomers of epoxy-2E-decenal<br />
Konrad Neumann<br />
In 2005, Konrad Neumann graduated at <strong>the</strong><br />
Technische Universität Berlin (TUB), Germany,<br />
as an Engineer (Dipl.-Ing.) of Biotechnology.<br />
Since 2005 he is working at <strong>the</strong> “Research<br />
and Teaching Institute <strong>for</strong> Brewing in Berlin”<br />
(VLB) in a research group of Prof. Tressl and<br />
Dr. Garbe. The focus of his work is <strong>the</strong> characterisation<br />
of reactive carbonyl compounds<br />
like 2E-nonenal, 2,4-decadienal and epoxydecenals<br />
and <strong>the</strong>ir interactions with peptides<br />
and proteins by means of GC-O, proton and<br />
carbon-13 NMR as well as LC-ESI-MS.
P 050<br />
Polyfunctional thiols in aged beer:<br />
Focus on <strong>the</strong> ribes off - fl avour<br />
Thi Thu Hang Tran 1, Jacques Gros 1,<br />
Sonia Collin 1<br />
1Université catholique de Louvain, Unité de brasserie et<br />
des industries alimentaires, Earth and Life Institute ELIM,<br />
Louvain-la-Neuve, Belgium<br />
DESCRIPTION OF TOPIC:<br />
For brewers, <strong>the</strong> term “ribes” refers to a characteristic<br />
taint encountered in some aged<br />
beers submitted to oxidation. A very strong<br />
odorant thiol, 3-sulfanyl-3-methylbutyl <strong>for</strong>mate<br />
(3S3MB-F) was <strong>here</strong> identifi ed as responsible<br />
<strong>for</strong> this blackcurrant/catty note. The<br />
sensorial threshold of 3S3MB-F was much<br />
lower (57 ng/L in beer) than that measured<br />
<strong>for</strong> its acetate analog. Thiol selective pHMB<br />
extraction was applied to a large number of<br />
commercial and pilot beers (lager and topfermented<br />
beers). The extracts were analysed<br />
by GC-O/PFPD/MS. Up to 629 ng/L of 3S3MB-<br />
F were detected in commercial lager beers<br />
after 1 month of natural ageing. The highest<br />
concentrations (1230 ng/L) were found in<br />
top-fermented beers with higher oxygen at<br />
bottling. Accelerated ageing on model media<br />
confi rmed <strong>the</strong> key role of oxygen.<br />
Thi Thu Hang Tran<br />
After obtaining her MSc degree at <strong>the</strong> Hanoi<br />
University of Science and Technology (Vietnam)<br />
in 2002, Thi-Thu-Hang Tran works as a<br />
lecturer and researcher at <strong>the</strong> Faculty of Food<br />
Science and Technology, Hanoi University of<br />
Agriculture. Since 2009, she has been a PhD<br />
student under <strong>the</strong> supervision of Prof. Sonia<br />
Collin at <strong>the</strong> <strong>Brewery</strong> and Food Chemistry<br />
Laboratory, Université catholique de Louvain,<br />
Belgium. Her research focuses on <strong>the</strong> polyfunctional<br />
thiols in beer through ageing.<br />
P 051<br />
Release of hop fl avours from cysteine<br />
conjugates by apotryptophanase<br />
Sonia Collin 1, Jacques Gros 1, Thi Thu Hang<br />
Tran 1<br />
1ELI - M (Applied Microbiology) / Université catholique de<br />
Louvain, Laboratoire de brasserie et des industries alimentaires<br />
(INBR), Louvain-la-Neuve, Belgium<br />
DESCRIPTION OF TOPIC:<br />
Polyfunctional thiols are contributors to <strong>the</strong><br />
hop varietal aroma of beer. Besides free thiols,<br />
cysteine-S-conjugates have been evidenced<br />
as additional components of <strong>the</strong> thiol<br />
potential of hop. Such cysteine adducts are<br />
investigated <strong>here</strong> in 8 hop cultivars (Amarillo,<br />
Cascade, Citra, Mosaic, Nelson Sauvin,<br />
Saaz, Simcoe, Tomahawk) and in diff erent<br />
hop <strong>for</strong>ms. Hop hydroalcoholic extracts were<br />
purifi ed on a cation exchanger and subjected<br />
to apotryptophanase beta-lyase activity.<br />
Several thiols were released by <strong>the</strong> enzymatic<br />
treatment, including <strong>the</strong> skunky-like<br />
3-methyl-2-buten-1-thiol, <strong>the</strong> box-tree-like<br />
4-sulfanyl-4-methylpentan-2-one, and <strong>the</strong><br />
grapefruit-like 3-sulfanylhexan-1-ol. Mosaic<br />
and Cascade hop varieties exhibited <strong>the</strong> highest<br />
bound 4-sulfanyl-4-methylpentan-2-one<br />
and 3-sulfanylhexan-1-ol potentials, respectivelly,<br />
while <strong>the</strong> Amarillo and Tomahawk cultivars<br />
were confi rmed to be important source<br />
of skunky-like thiols. Surprisingly, hop CO 2 extracts<br />
proved to contain cysteine conjugates.<br />
Sonia Collin<br />
In 1988, Sonia COLLIN received a Ph.D degree<br />
in chemical sciences. She started her career<br />
as Chercheur Qualifi é at <strong>the</strong> FNRS. Professeur<br />
ordinaire at <strong>the</strong> Université catholique de<br />
Louvain (Belgium), she is responsible of <strong>the</strong><br />
Master in Brewing Sciences. She chaired <strong>the</strong><br />
last De Clerck symposium devoted to <strong>the</strong> ‚Belgian<br />
beer paradox‘. She has published more<br />
than 100 publications in peer-reviews, mainly<br />
on fl avour stability, sulfur aroma, pyrazines,<br />
hops, and polyphenols (http://www.uclouvain.<br />
be/inbr).<br />
P 052<br />
Odorant phenolic markers in chocolate<br />
malts. Fate of <strong>the</strong>m through<br />
beer ageing<br />
Caroline Scholtes 1, Sonia Collin 1<br />
1Université catholique de Louvain, ELIM - Laboratoire de<br />
brasserie et des industries alimentaires, Louvain-la-Neuve,<br />
Belgium<br />
DESCRIPTION OF TOPIC:<br />
Issued from enzymatic or <strong>the</strong>rmic decarboxylation<br />
of phenolic acids, volatile phenols are<br />
responsible of typical spicy notes in a large<br />
range of beverages. Among <strong>the</strong>se, brown special<br />
beers, exhaling strong coff ee aromas, generally<br />
contain dark specialty malts, produced<br />
at higher kilning temperatures than typical<br />
pilsen malts and through specifi c equipment<br />
like roasting drum or torrefactor. A panel of<br />
malts (4,5 to 1500 EBC) were analysed and<br />
<strong>the</strong> resulting phenol-specifi c extracts investigated<br />
by gas chromatography - olfactometry<br />
(GC-O/AEDA) and mass spectrometry<br />
(GC-MS). Diff erent profi les emerged from this<br />
study, according to <strong>the</strong> temperature-duration<br />
conditions applied. Interesting markers were<br />
highlighted in torrefi ed/chocolate malts and<br />
derived beers such as guaiacol. Moreover, investigation<br />
of aged beers revealed and unexpected<br />
hidden potential of guaiacol and analogs,<br />
progressively liberated through storage<br />
by acid hydrolysis.<br />
Caroline Scholtes<br />
Graduated in 2007 as Bio-Engineer from Université<br />
Catholique de Louvain (Belgium), Caroline<br />
Scholtes has completed her education<br />
in 2008 with a master in Brewing Science.<br />
In 2009, she started a Ph.D. program at <strong>the</strong><br />
same university. Her research focuses on<br />
ageing of special Belgian beers regarding to<br />
raw materials, brewing process and storage<br />
conditions and correlates this to evolution of<br />
organoleptic profi le, especially Madeira off -<br />
fl avor and volatile phenols.<br />
61 34 TH EBC CONGRESS · LUXEMBOURG 26 – 30 MAY 2013
P 053<br />
Sake fermentations:<br />
what can brewers learn<br />
Daniel Kerruish 1, Trevor Phister 1,<br />
Ka<strong>the</strong>rine Smart 2<br />
1University of Nottingham, Nottingham, United Kingdom,<br />
2SABMiller plc, Woking, United Kingdom<br />
DESCRIPTION OF TOPIC:<br />
Sake fermentations use rice, water and a<br />
co-culture of Aspergillus oryzae (Koji) and<br />
Saccharomyces cerevisiae (Kyokai) to achieve<br />
ethanol yields of up to 22%. Sake fermentations<br />
derive fermentable sugars from <strong>the</strong><br />
breakdown of polished rice by <strong>the</strong> exogenous<br />
enzymes produced by Aspergillus oryzae.<br />
These fermentable sugars are <strong>the</strong>n utilised by<br />
S. cerevisiae. Sake fermentations are t<strong>here</strong><strong>for</strong>e<br />
complex in nature and poorly understood.<br />
The purpose of this work is to understand <strong>the</strong><br />
reasons why Sake yeast are apparently so<br />
ethanol yielding and ethanol tolerant.<br />
T<strong>here</strong> are several possible reasons <strong>for</strong> this:<br />
fermentations are conducted with Aspergillus<br />
oryzae this mixed fermentation may be benefi<br />
cial to <strong>the</strong> yeast; Sake yeast strains may<br />
also have a more eff ective ethanol defence. In<br />
this presentation <strong>the</strong>se two hypo<strong>the</strong>ses will<br />
be discussed.<br />
Daniel Kerruish<br />
Having gained a degree in microbiology from<br />
Liverpool University in 2001 and a PhD from<br />
Kings College London in 2007; Daniel spent 4<br />
years working as a technical brewer be<strong>for</strong>e<br />
fur<strong>the</strong>ring his research interests at <strong>the</strong> University<br />
of Nottingham. His current research<br />
interests are focussed upon sake fermentations<br />
and ethanol tolerance of yeast.<br />
P 054<br />
Unraveling <strong>the</strong> yeast fl occulation<br />
mechanism at <strong>the</strong> molecular level<br />
Francesco Ielasi 1, Katty Goossens 1,<br />
Ronnie Willaert 1<br />
1Vrije Universiteit Brussel, Brussels, Belgium<br />
DESCRIPTION OF TOPIC:<br />
Yeast cell fl occulation is exploited in <strong>the</strong> brewery<br />
industry as an easy and cost-eff ective<br />
way to separate <strong>the</strong> aggregated yeast cells<br />
from <strong>the</strong> beer at <strong>the</strong> end of <strong>the</strong> primary fermentation.<br />
The fl occulation mechanism is<br />
based on a lectin-carbohydrate interaction,<br />
but was until recently not yet fully clear. The<br />
Flo adhesin family, in particular FLO1 and Lg-<br />
FLO1 gene products are lectins responsible<br />
<strong>for</strong> <strong>the</strong> Ca2+-dependent yeast fl occulation<br />
phenotype. A biophysical study of <strong>the</strong> fl occulation<br />
mechanism on <strong>the</strong> molecular level was<br />
carried out. The N-terminal carbohydratebinding<br />
domains of Flo1p from <strong>the</strong> ale yeast<br />
Saccharomyces cerevisiae and Lg-Flo1p<br />
from <strong>the</strong> lager yeast S. pastorianus were produced<br />
from suitable recombinant expression<br />
systems. Later on, a combination of protein<br />
crystallography, kinetic binding experiments<br />
and Single-Molecule Force Spectroscopy allowed<br />
us to refi ne <strong>the</strong> model <strong>for</strong> <strong>the</strong> binding of<br />
<strong>the</strong>se proteins to yeast cell-wall glycans.<br />
Francesco Ielasi<br />
PhD student at <strong>the</strong> Vrije Universiteit Brussel<br />
- VUB<br />
62<br />
P 055<br />
Worldwide breeding strategy <strong>for</strong><br />
LOX-less barley and characteristics<br />
of new Australian malting barley<br />
variety ‚Sou<strong>the</strong>rnStar‘<br />
Makoto Kihara 1, Wataru Saito 1, Takehiro<br />
Hoki 1, Takashi Iimure 1, Naohiko Hirota 1,<br />
Masayuki Shimase 2, Kiyoshi Takoi 2, Jason<br />
Eglinton 3, Shinji Yamada 1<br />
1SAPPORO BREWERIES LTD., Bioresources Research and<br />
Development Department, Gunma, Japan, 2SAPPORO BRE-<br />
WERIES LTD., Product & Technology Innovation Department,<br />
Shizuoka, Japan, 3The University of Adelaide, School of<br />
Agriculture, Food and Wine, Waite Campus, Australia<br />
DESCRIPTION OF TOPIC:<br />
For years, people talk about <strong>the</strong> quality of<br />
draught beer and <strong>the</strong>ir potential <strong>for</strong> improvement.<br />
Developments in dispensing systems<br />
are, however, in most cases, just to improve<br />
<strong>the</strong> appearance of <strong>the</strong> equipment. The most<br />
important aspect <strong>for</strong> a brewer - hygiene - is<br />
often neglected. As a consequence, <strong>the</strong> quality<br />
of beer is destroyed in <strong>the</strong> last few meters,<br />
just be<strong>for</strong>e <strong>the</strong> consumer enjoys it.<br />
We carried out extensive studies on construction,<br />
cleaning and maintenance of dispensing<br />
equipment. It was found <strong>here</strong> that <strong>the</strong> microbial<br />
load in draught beer could be reduced by<br />
good hygiene practices in 6 weeks by more<br />
than 90%. The investigation of design aspects<br />
also brought strong improvements. Here also<br />
immense hygienic improvements were observed.<br />
The presentation shows <strong>the</strong>se fi ndings and<br />
gives advice, such as fast recontaminations<br />
can be avoided and <strong>the</strong> draught beer quality<br />
can be improved. The presentation also will<br />
show <strong>the</strong> growth and sensorial infl uence of<br />
microbial infections in draught beer.<br />
Makoto Kihara<br />
Since 1990: Research Scientist in <strong>the</strong> Plant<br />
Bioengineering Research Laboratories, Sapporo<br />
Breweries Ltd., Since 2009: Chief of Barley<br />
R&D center in <strong>the</strong> Bioresources Research<br />
and Development Department, Sapporo Breweries<br />
Ltd.. Since 1999: Doctor (Title of doctor<br />
<strong>the</strong>sis: Establishment of technique <strong>for</strong> <strong>the</strong><br />
production of transgenic plants using protoplast<br />
culture system in barley).
P 056<br />
All-malt beer vs. non all-malt:<br />
how much corn does it take to<br />
make a diff erence? (industrial tests)<br />
Aleksander Poreda 1, Marek Zdaniewicz 1,<br />
Piotr Antkiewicz 1<br />
1University of Agriculture in Krakow, Department of Fermentation<br />
Technology and Technical Microbiology, Krakow, Poland<br />
DESCRIPTION OF TOPIC:<br />
Unmalted adjuncts are used in brewing technology<br />
<strong>for</strong> number of reasons. Sometimes <strong>the</strong><br />
intention is to modify <strong>the</strong> profi le of an „original“<br />
beer, o<strong>the</strong>r times we aim at gaining a „no<br />
diff erence“ beer.<br />
In this work we investigated <strong>the</strong> impact of<br />
corn grist on <strong>the</strong> wort parameters, fermentation<br />
per<strong>for</strong>mance and <strong>the</strong> quality of beer.<br />
The experiments were per<strong>for</strong>med in full industrial<br />
scale. The main scope was to answer<br />
<strong>the</strong> question: if and how <strong>the</strong> addition of corn<br />
changes <strong>the</strong> quality of beer. In order to better<br />
understand <strong>the</strong> infl uence of corn it was<br />
used in various amounts (up to 20%) and we<br />
per<strong>for</strong>med full analysis not only on <strong>the</strong> beer<br />
(physico-chemical, GC and trained sensory<br />
panel) but also on intermediates (wort and<br />
young beer). In <strong>the</strong> case of positive results of<br />
triangle test (all-malt beer vs. beer produced<br />
with corn adjunct), <strong>the</strong> panel was asked to<br />
make a full description of <strong>the</strong> beer samples<br />
to gain knowledge on <strong>the</strong> specifi c role of corn<br />
adjuncts in creation of <strong>the</strong> sensory profi le of<br />
beer.<br />
Aleksander Poreda<br />
Aleksander Poreda works at <strong>the</strong> University of<br />
Agriculture in Krakow (Poland), specializing<br />
in brewing technology at <strong>the</strong> Faculty of Food<br />
Technology. In his PhD Aleksander focused on<br />
<strong>the</strong> role of metal ions in <strong>the</strong> fermentation process,<br />
analysed <strong>the</strong> uptake and release of metal<br />
ions by yeast in industrial conditions. For<br />
last few years he has been involved in many<br />
research projects run with cooperation with<br />
major breweries located in Poland.<br />
P 057<br />
Hops:<br />
New opportunities <strong>for</strong> brewers<br />
Philippe Janssens 1<br />
1Yakima Chief Inc., YC-Specialties Division, Louvain-la-Neuve,<br />
Belgium<br />
DESCRIPTION OF TOPIC:<br />
The purpose of <strong>the</strong> lecture is to study <strong>the</strong> relationship<br />
between hops and beer fl avor to<br />
create new opportunities <strong>for</strong> brewers.<br />
The fi rst part will be focused on <strong>the</strong> intensity<br />
and <strong>the</strong> quality of <strong>the</strong> bitterness. Diff erent<br />
trans<strong>for</strong>mations occur during <strong>the</strong> wort boiling,<br />
which impact on <strong>the</strong> bitterness. Some<br />
hop-derived compounds have a bad impact<br />
on <strong>the</strong> bitterness. Hop breeders have been<br />
very active to develop new high alpha hop<br />
varieties. Reduced <strong>for</strong>ms of Iso-alpha acids<br />
also exist with diff erent properties and functionalities.<br />
The second part will be focused on <strong>the</strong> hop<br />
aroma. The hop composition and major hop<br />
compounds will be reviewed. Hop varieties<br />
(e.g. traditional hop varieties and specialty<br />
hop varieties) and <strong>the</strong>ir contribution in <strong>the</strong><br />
hop aroma will be investigated. Finally, <strong>the</strong><br />
points of addition of hops in <strong>the</strong> brewing process<br />
and <strong>the</strong> impact on <strong>the</strong> hop aroma will<br />
be discussed at high temperature and in presence<br />
of yeast.<br />
Hop fl avor = Hop Variety X Quantity X Process<br />
Philippe Janssens<br />
Philippe Janssens is Bio-engineer, with a<br />
master in malting and brewing sciences from<br />
UCL University. He had been working <strong>for</strong> 15<br />
years in R&D <strong>for</strong> InBev, w<strong>here</strong> he gets experiences<br />
in a wide various areas. After, he joined<br />
<strong>the</strong> IP department of InBev as Patent Liaison<br />
Offi cer <strong>for</strong> 4 years be<strong>for</strong>e joining Bird Goën &<br />
Co, a Private Patent Law fi rm, w<strong>here</strong> he was<br />
responsible <strong>for</strong> food industry technologies.<br />
He is now responsible <strong>for</strong> all technical and<br />
development aspects of hops within Yakima<br />
Chief, Inc.<br />
P 058<br />
Comparison of hop growing areas<br />
José Luis Olmedo 1, Luis Alvar 2<br />
1Hijos de Rivera, S.A., R&D, La Coruña, Spain, 2Hijos de Rivera,<br />
S.A., Production, La Coruña, Spain<br />
DESCRIPTION OF TOPIC:<br />
Brewers have a general idea of <strong>the</strong> soil‘s and<br />
climate‘s infl uence in a given hop growing<br />
area with specifi c notes imparted to products<br />
of <strong>the</strong> same variety cultivated in diff erent regions.<br />
During this research we studied 9 diff erent<br />
varieties of hops in three consecutive crops:<br />
Nugget, Columbus, Magnum, Merkur, Perle,<br />
Taurus, Mittelfruher, Saaz and Sladek and in<br />
areas such as: Spain (Galicia and León), Germany,<br />
USA and Czech Republic.<br />
Parameters analyzed: humidity, acids (cohumulone,<br />
adhumulone+humulone), acids (colupulone,<br />
adlupulone+lupulone), polyphenols,<br />
fl avonoids and xantohumol by <strong>the</strong> University<br />
of Santiago de Compostela following standardized<br />
methods of <strong>the</strong> <strong>European</strong> <strong>Brewery</strong> Convention<br />
(in some cases with HPLC).<br />
Some interesting diff erences in composition<br />
were found in <strong>the</strong> relationship with <strong>the</strong> specifi<br />
c growing location, opening up new possibilities<br />
to develop new beers with this raw<br />
material.<br />
With fi nancial support from ERDF by <strong>the</strong><br />
Consellería de Economía e Industria.<br />
José Luis Olmedo<br />
Born in Madrid in 1971. Studied engineering<br />
in agronomy at <strong>the</strong> University of León (Spain)<br />
until 1998. Study a Master‘s Degree in malting<br />
and brewing technology at <strong>the</strong> Politechnic<br />
University of Madrid in 1999. From 1999 working<br />
in Hijos de Rivera, S.A. as brewmaster,<br />
market quality management and nowadays in<br />
<strong>the</strong> R&D department. Project Manager of several<br />
projects such us: raw materials (including<br />
hops), new <strong>for</strong>mats, new poducts (beer<br />
recipes) and development of technology.<br />
63 34 TH EBC CONGRESS · LUXEMBOURG 26 – 30 MAY 2013
P 059<br />
Monitoring <strong>the</strong> appearance and<br />
composition of lupulin glands<br />
during maturation up to and beyond<br />
harvest<br />
Keith Westwood 1, Katarzyna Wolinska 1<br />
1Barth Innovations Limited, Tonbridge, United Kingdom<br />
DESCRIPTION OF TOPIC:<br />
Many factors determine <strong>the</strong> optimum date <strong>for</strong><br />
harvesting hops. The decision relies on <strong>the</strong> experience<br />
of <strong>the</strong> grower and it is rare <strong>for</strong> more<br />
objective (expensive) analytical methods to be<br />
used. The application of a simple, inexpensive<br />
analytical procedure would give <strong>the</strong> growers<br />
more insight into <strong>the</strong> maturity of <strong>the</strong> crop and<br />
aid <strong>the</strong> decision when to harvest. In this study<br />
two varieties of hops were monitored, up to<br />
and beyond harvest date. The appearance of<br />
<strong>the</strong> lupulin glands was observed using a simple<br />
microscope and <strong>the</strong> hops analysed <strong>for</strong> alpha<br />
acids, oil content and composition. As <strong>the</strong><br />
crop developed, lupulin glands changed and<br />
when a dark, opaque tip appeared this was<br />
a good indication <strong>for</strong> optimum harvesting. At<br />
this point <strong>the</strong> oil content was signifi cantly higher<br />
than at time of harvest, whilst <strong>the</strong> alpha<br />
acid levels remained fairly stable. It appears<br />
that <strong>for</strong> <strong>the</strong> particular growths monitored, <strong>the</strong><br />
hops were harvested 10-12 days too early <strong>for</strong><br />
optimum fl avour impact.<br />
Keith Westwood<br />
Keith Westwood gained his PhD in Chemistry<br />
in 1982, be<strong>for</strong>e joining <strong>the</strong> Brewing Research<br />
Foundation, working on hop based projects,<br />
including fractionation of hop oil and isomerised<br />
hop extracts. In 1991, Keith joined Hop<br />
Developments in Eardiston, as Technical Director<br />
and ultimately in Botanix Ltd. In 2010,<br />
Keith was asked to lead a new innovations<br />
company, Barth Innovations Ltd, which is responsible<br />
implementing a R&D program, to<br />
meet <strong>the</strong> needs of <strong>the</strong> Barth Group of companies<br />
in Europe.<br />
P 060<br />
Infl uence of picking date on <strong>the</strong> Hop<br />
Storage Index (HSI)<br />
Sandro Cocuzza 1, Rasso Hiltmann 2,<br />
Maximilian Krause 2<br />
1Hopsteiner, Mainburg, Germany, 2TUM Weihenstephan,<br />
Freising, Germany<br />
DESCRIPTION OF TOPIC:<br />
HSI is one of <strong>the</strong> parameters to evaluate<br />
freshness of hops and hop products. Upper<br />
control limit values <strong>for</strong> hop purchasing are<br />
often set up in <strong>the</strong> range of 0.35 to 0.45. Fresh<br />
harvested hops are assessed to have an HSI<br />
around 0.25.<br />
Internal analyses of fresh harvested hops<br />
over <strong>the</strong> last crop years indicated both inconsistent<br />
initial HSI <strong>for</strong> all varieties within<br />
<strong>the</strong> same crop year and also varying values<br />
within one variety over several years. To clarify<br />
if picking date infl uences <strong>the</strong> initial HSI,<br />
standardized harvesting trials were per<strong>for</strong>med<br />
in cooperation with <strong>the</strong> Research Centres<br />
at Hüll and Weihenstephan. Samples of<br />
3 aroma and bitter varieties from 2 locations<br />
were taken over an extended period around<br />
<strong>the</strong> regular picking date.<br />
The results show that HSI increases with later<br />
picking date. For <strong>the</strong> evaluation of hop freshness,<br />
annual and varietal fl uctuations should<br />
be considered and t<strong>here</strong><strong>for</strong>e <strong>the</strong> increase of<br />
HSI from its initial value („Δ-HSI“) is more suitable<br />
than fi xed values.<br />
Sandro Cocuzza<br />
Sandro Cocuzza graduated in 2007 as an<br />
Engineer in Brewing and Beverage Technology<br />
at <strong>the</strong> Technical University of Munich-<br />
Weihenstephan. Afterwards he worked as a<br />
researcher in <strong>the</strong> Department of Microbiology<br />
in Weihenstephan. In 2008 he joined <strong>the</strong><br />
Hopsteiner Group as Technical Support Manager.<br />
64<br />
P 061<br />
Gushing induced by hop acid components<br />
- importance in various<br />
beers and beverages<br />
Brian Buffi n 1<br />
1Kalsec, Inc., Kalamazoo, United States<br />
DESCRIPTION OF TOPIC:<br />
The occurrence and control of gushing is an<br />
important quality factor. Eff orts to identify<br />
gushing promoters have focused on barley<br />
degradation, and, to a lesser extent, hop constituents.<br />
The use of pre-isomerized hop acids, including<br />
reduced <strong>for</strong>ms, continues to increase. We<br />
previously reported <strong>the</strong> results from a study<br />
that evaluated <strong>the</strong> impact of advanced hop<br />
products on gushing in high-volume American<br />
lagers. The existence and importance of hop<br />
acid induced gushing has now been evaluated<br />
in diff erent carbonated and malt beverages.<br />
It was shown that <strong>the</strong> nature of <strong>the</strong> beverage<br />
has a dramatic impact on <strong>the</strong> presence and<br />
extent of gushing. Research was conducted to<br />
identify <strong>the</strong> infl uence of hop acids, hop processing,<br />
beverage type, and dosing levels.<br />
Experimental details and <strong>the</strong> relevance to<br />
manufacturers of beer and o<strong>the</strong>r carbonated<br />
beverages will be presented. Given <strong>the</strong> increasing<br />
interest in hop bittering components <strong>for</strong><br />
a variety of products, this work should appeal<br />
to a broad audience.<br />
Brian Buffi n<br />
Brian Buffi n is <strong>the</strong> Director of Hops Research<br />
and Development <strong>for</strong> Kalsec®, Inc., a leader in<br />
<strong>the</strong> supply of advanced hop products to <strong>the</strong><br />
brewing industry. Dr. Buffi n holds Ph.D. and<br />
M.S. degrees in Organometallic Chemistry<br />
from <strong>the</strong> University of Utah and a B.S. degree<br />
in Chemistry from Calvin College. After working<br />
in <strong>the</strong> diatomaceous earth industry <strong>for</strong><br />
World Minerals/Celite, he spent over 13 years<br />
in professorships at academic institutions be<strong>for</strong>e<br />
joining Kalsec® in 2008.
P 062<br />
Use of <strong>the</strong>rmal characterization<br />
during malting process<br />
Luciana Machado de Oliveira 1,2, Marco<br />
Aurélio Da Silva Carvalho Filho 2, Carlos<br />
Sanchuki 3, Eliane Carvalho de Vasconcelos 2,<br />
Egon Schnitzler 4, Ivo Mottin Demiate 5, Luiz<br />
Gustavo Lacerda 2<br />
1SENAI, Food Quality, Curitiba, Brazil, 2Universidade Positivo,<br />
Biotechnology, Curitiba, Brazil, 3SENAI, Biotechnology, Curitiba,<br />
Brazil, 4Universidade Estadual de Ponta Grossa, MSc Food<br />
Technology, Ponta Grossa, Brazil, 5Universidade Estadual de<br />
Ponta Grossa, Food Engineering, Ponta Grossa, Brazil<br />
DESCRIPTION OF TOPIC:<br />
Malt used in breweries is obtained from barley:<br />
a very known cereal used by Neolithic<br />
cultures in Egypt since 6000 B.C.. It is a grass<br />
belonging to <strong>the</strong> genus Hordeum. As a tool <strong>for</strong><br />
characterization and quality control of natural<br />
materials, Thermal Analysis (TA) reached a<br />
very high level of sophistication due to progress<br />
of Science and Technology. In this study<br />
we analyzed structures of barley during malting<br />
to understand characteristics and to evaluate<br />
physical-chemical properties of <strong>the</strong> process.<br />
Thermogravimety, Diff erential <strong>the</strong>rmal<br />
analyses, Diff erential Scanning Calorimetry<br />
and Optical Microscopy were carried out. Enzymes<br />
have preference to attack amorphous<br />
areas on starch surface. This observation<br />
could explain <strong>the</strong> fact that treated material<br />
had aff ected areas due to <strong>the</strong> enzyme action.<br />
Fur<strong>the</strong>rmore, TA showed an increasing in<br />
both <strong>the</strong>rmal stability and required energy <strong>for</strong><br />
transitions during malting.<br />
Luciana Machado de Oliveira<br />
BSc in Nutrition, attending MSc in Industrial<br />
Biotechnology at Universidade Positivo.<br />
Teaches at SENAI.<br />
P 063<br />
Impact of barley variety and malting<br />
process on wort amino acid profi le<br />
and content<br />
Marc Schmitt 1, Patrick Boivin 2<br />
1IFBM, R&D, Vandoeuvre, France, 2IFBM, Vandoeuvre, France<br />
DESCRIPTION OF TOPIC:<br />
FAN content of wort is measured as indicator<br />
of wort suitability <strong>for</strong> fermentation. It consists<br />
of individual amino acids, small peptides and<br />
ammonia. It is sometimes not related with<br />
fermentation per<strong>for</strong>mance. The concentration<br />
of individual AA in wort can aff ect both <strong>the</strong><br />
rate of fermentation and <strong>the</strong> fl avour production<br />
by yeast.<br />
The purpose of this work is to study <strong>the</strong> impact<br />
of barley variety and malting process on<br />
<strong>the</strong> amino acid profi le of wort.<br />
O<strong>the</strong>r three crops with samples from diff erent<br />
areas in France, both winter and spring<br />
varieties were tested. A signifi cant impact of<br />
<strong>the</strong> variety was shown, but <strong>the</strong> crop has also<br />
an infl uence. The diff erences of proteolysis<br />
levels between cultivars couldn‘t explain <strong>the</strong><br />
AA composition of wort.<br />
We also tested <strong>the</strong> infl uence of malting conditions<br />
on AA composition of wort. It was shown<br />
<strong>the</strong> malting conditions have an impact both on<br />
content and on composition of wort. The varieties<br />
tested didn‘t have <strong>the</strong> same sensitivity to<br />
malting conditions.<br />
Marc Schmitt<br />
PhD in Foood Biochemistry. Raw materials<br />
qualifi cation and biochemistry R&D manager<br />
at IFBM since 2008. Laboratory manager from<br />
2002 to 2008.<br />
P 064<br />
A possibility to decrease gluten in<br />
barley malt based beverages by<br />
using barley malt with optimized<br />
celiac-specifi c peptidase activity<br />
Roland Kerpes 1, Verena Knorr 2, Martin<br />
Zarnkow 1, Peter Köhler 2, Thomas Becker 1<br />
1Lehrstuhl für Brau- und Getränketechnologie, Technische<br />
Universität München, Freising, Germany, 2Deutsche Forschungsanstalt<br />
für Lebensmittelchemie, Freising, Germany<br />
DESCRIPTION OF TOPIC:<br />
Celiac disease is one of <strong>the</strong> most frequent<br />
occurring intestinal diseases worldwide. It is<br />
initiated by <strong>the</strong> storage proteins of barley, rye<br />
and wheat, which are termed gluten in terms<br />
of celiac disease. One way of reducing gluten<br />
content in fi nal beer is to increase <strong>the</strong> proteolytic<br />
namely prolyl endopeptidase activity in<br />
germinating grain while considering <strong>the</strong> infl uence<br />
on standard quality attributes. T<strong>here</strong><strong>for</strong>e<br />
<strong>the</strong> aim of this study was to investigate <strong>the</strong><br />
impact of varying malting conditions (moisture,<br />
temperature and time) on peptidase<br />
activity of barley using Response Surface Methodology.<br />
Fur<strong>the</strong>rmore standard brewing attributes<br />
such as extract, free amino nitrogen,<br />
kolbach index, total nitrogen and viscosity<br />
were analyzed. All measured attributes were<br />
signifi cantly aff ected. In <strong>the</strong> next step standard<br />
brewing procedures will be investigated<br />
to gain fundamental insight of what gluten is<br />
left in beer as a basis <strong>for</strong> <strong>the</strong> application of <strong>the</strong><br />
optimized barley malt or barley malt extract.<br />
Roland Kerpes<br />
Roland Kerpes studied brewing science and<br />
beverage technology at <strong>the</strong> Technische Universität<br />
München (TUM). He fi nished his diploma<br />
<strong>the</strong>sis about <strong>the</strong> impact of unmalted oats<br />
on <strong>the</strong> quality and processability of mashes,<br />
worts, and beers in 2011. This study was<br />
carried out under cooperative supervision of<br />
Prof. Dr.-Ing. Thomas Becker, TUM and Prof.<br />
Dr. Elke Arendt, UCC - Ireland. Since 2011<br />
he´s working on <strong>the</strong> development of a glutenfree<br />
beer by <strong>the</strong> use of a peptidase enriched<br />
malt at <strong>the</strong> BGT, TUM.<br />
65 34 TH EBC CONGRESS · LUXEMBOURG 26 – 30 MAY 2013
P 065<br />
Profi ling of metabolites‘ and aroma<br />
developments during kilning<br />
Karin Gorzolka 1, Karsten Niehaus 1<br />
1Bielefeld University, Proteome and Metabolome Research,<br />
Bielefeld, Germany<br />
DESCRIPTION OF TOPIC:<br />
Kilning of barley seeds serves two aims: Stop<br />
of germination events and aroma development.<br />
In industrial kilns barley seeds are exposed<br />
to diff erent temperature and moisture<br />
conditions, depending on <strong>the</strong>ir position in <strong>the</strong><br />
grain bed.<br />
Samples were taken during kilning from <strong>the</strong><br />
top, middle and bottom. Analysis of aroma<br />
profi les by SPME-GC-MS revealed more than<br />
40 compounds. Metabolite profi ling by GC-MS<br />
served to evaluate germination proceeding<br />
and to detect aroma precursors. 69 hydrophilic<br />
metabolites were identifi ed.<br />
Grain in <strong>the</strong> upper kiln showed ongoing germination<br />
during <strong>the</strong> fi rst 18 hours, w<strong>here</strong>as<br />
seed metabolomes at <strong>the</strong> bottom were stable<br />
after 3 hours. Increased temperature in <strong>the</strong><br />
second phase of kilning fur<strong>the</strong>r changed metabolite<br />
patterns, but in smaller extents. Aroma<br />
profi les distinguished between wet and<br />
dry samples. Detailled profi ling revealed fi ner<br />
aroma developments during roasting, which<br />
was consistent with observations made in<br />
metabolite profi les.<br />
Karin Gorzolka<br />
Karin Gorzolka studied biology in Bielefeld,<br />
Germany, with special focus on molecular<br />
biology. After receiving her Diploma in 2009,<br />
she started her PhD <strong>the</strong>sis which is about<br />
metabolome and proteome profi ling of barley<br />
during malting. Using <strong>the</strong> plat<strong>for</strong>ms of MALDI<br />
mass spectrometry and gas chromatography<br />
mass spectrometry she focusses on MS<br />
based methods.<br />
P 066<br />
Predicting malt quality from barley<br />
gene expression<br />
Tommi Suvitaival1, Annika Wilhelmson1, Gillberg Jussi2, Jari Rautio3, Oona Rechardt3, Alan Schulman4, Jaakko Tanskanen5, Ulla Holopainen1, Esko Pajunen6, Pekka<br />
Reinikainen7, Anneli Ritala1 1VTT Technical Research Centre of Finland, Espoo, Finland,<br />
2Aalto University, Espoo, Finland, 3Plexpress Oy, Helsinki, Finland,<br />
4University of Helsinki, Institute of Biotechnology, Helsinki,<br />
Finland, 5MTT Agrifood Research Finland, Biotechnology<br />
and Food Research, Helsinki, Finland, 6Oy Sinebrychoff Ab,<br />
Kerava, Finland, 7Viking Malt Oy, Lahti, Finland<br />
DESCRIPTION OF TOPIC:<br />
We identifi ed barley genes expressed during<br />
malting associated with quality, process per<strong>for</strong>mance<br />
and yield. Three barley varieties<br />
from two growth environments were micromalted.<br />
Gene expression during malting was<br />
studied using microarrays. Malt quality was<br />
analysed and linked to gene expression data<br />
using bioin<strong>for</strong>matic and statistical approaches.<br />
Regularized regression models were<br />
used to select groups of about 20 genes to<br />
predict relevant quality parameters; <strong>the</strong> most<br />
reliable model was obtained <strong>for</strong> FAN. A probabilistic<br />
model revealed quality parameters<br />
that were explained mainly by variety and<br />
growth location. For example, wort haze and<br />
colour were strongly aff ected by growth environment,<br />
and friability was aff ected by variety.<br />
Transcriptional profi ling by affi nity capture<br />
was used to evaluate <strong>the</strong> predictive power of<br />
<strong>the</strong> models. Fur<strong>the</strong>r work on <strong>the</strong> identifi cation<br />
of malt quality-related genes will benefi t from<br />
<strong>the</strong> future completion of <strong>the</strong> barley genome.<br />
Tommi Suvitaival<br />
Tommi Suvitaival is a doctoral student at<br />
Helsinki Institute <strong>for</strong> In<strong>for</strong>mation Technology<br />
HIIT, Department of In<strong>for</strong>mation and Computer<br />
Science, Aalto University. He graduated<br />
as Master of Science in Technology from Helsinki<br />
University of Technology in 2009. His<br />
research interests include machine learning<br />
methods <strong>for</strong> computational biology, particularly<br />
Bayesian modeling.<br />
66<br />
P 067<br />
Accurate, quick and simple - Innovative<br />
level measurement of grain<br />
and malt<br />
Joachim Koelsch1 1Siemens AG, Karlsruhe, Germany<br />
DESCRIPTION OF TOPIC:<br />
Level measurement of grain and malt - requirements<br />
Overview of diff erent technologies<br />
Strengths and weaknesses of <strong>the</strong> diff erent<br />
technologies<br />
Application requirements<br />
New radar technology <strong>for</strong> solids level measurement<br />
Benefi ts of <strong>the</strong> new radar technology<br />
Application experiences within <strong>the</strong> grain and<br />
malt industry<br />
Joachim Koelsch<br />
• 8 years Regional Sales Manager <strong>for</strong> Siemens<br />
Process Instrumentation in Germany.<br />
• Since 2008 Industry Business Development<br />
Food & Beverage <strong>for</strong> Siemens Process Instrumentation<br />
Be<strong>for</strong>e joining Siemens in 2000:<br />
• Study of Brewing Technology at TU/VLB<br />
Berlin<br />
• 6 years Regional Sales Manager <strong>for</strong> KHS in<br />
Germany in sales of bottling plants<br />
• 6 years Regional Sales Manager <strong>for</strong> Tetra<br />
Pak Processing in Germany in sales of food<br />
& beverage processing plants
P 068<br />
Impact of unmalted white and red<br />
sorghum [Sorghum bicolor (L.)<br />
Moench] on <strong>the</strong> quality and processability<br />
of mashes, worts and<br />
beers<br />
Birgit Schnitzenbaumer 1, Carina Karl 1,<br />
Fritz Jacob 2, Elke K. Arendt 1<br />
1University College Cork, School of Food and Nutritional<br />
Sciences, Cork, Ireland, 2Technische Universität München,<br />
Research Center Weihenstephan <strong>for</strong> Brewing and Food<br />
Quality, Freising, Germany<br />
DESCRIPTION OF TOPIC:<br />
The use of unmalted sorghum in brewing<br />
usually involves <strong>the</strong> application of commercial<br />
enzymes. However, a balance between<br />
product quality and production costs has to<br />
be established. The aim of this study was to<br />
evaluate <strong>the</strong> quality and processability of mashes,<br />
worts and beers brewed with 40% white<br />
or red sorghum and optimised enzyme levels.<br />
Brewing with sorghum adjunct was carried<br />
out in a 60-L pilot plant using a double infusion<br />
process. The per<strong>for</strong>mance of white or red<br />
sorghum during mashing, lautering and fermentation<br />
was monitored and <strong>the</strong> quality of<br />
worts and beers was analysed in detail using<br />
standard methods specifi ed by MEBAK or EBC<br />
as well as Lab-on-a-Chip capillary electrophoresis.<br />
All brewing trials were per<strong>for</strong>med<br />
in duplicate. Signifi cant diff erences in quality<br />
and processability of mashes, worts and<br />
beers produced with 40% white or red sorghum<br />
adjunct were revealed. The optimised<br />
use of commercial enzymes has <strong>the</strong> ability<br />
to improve <strong>the</strong> beer quality and to reduce <strong>the</strong><br />
costs.<br />
Birgit Schnitzenbaumer<br />
Birgit Schnitzenbaumer studied Brewing and<br />
Beverage Technology at <strong>the</strong> Technical University<br />
of Munich in Weihenstephan. She completed<br />
several internships in diff erent German<br />
breweries and did her Master‘s <strong>the</strong>sis at<br />
<strong>the</strong> University College Cork in Ireland. Birgit<br />
graduated as Dipl.-Ing. (M.Sc.) in Brewing and<br />
Beverage Technology in 2009 and was awarded<br />
an InBev-Baillet Latour Scholarship <strong>for</strong><br />
doctoral students. She is currently working<br />
towards a Ph.D. degree at <strong>the</strong> University College<br />
Cork, Ireland.<br />
P 069<br />
Screening of fl avour compounds in<br />
beer and brewing materials using<br />
TRP channel assay<br />
Chizuru Sato 1, Hisao Kuroda 1, Tatsuro<br />
Shigyo 1<br />
1SAPPORO BREWERIES LTD., Frontier Laboratories of Value<br />
Creation, Yaizu, Japan<br />
DESCRIPTION OF TOPIC:<br />
Beer contains hundreds of compounds that<br />
contribute to its fl avour characteristics. T<strong>here</strong><br />
are many reports concerning aroma of beer<br />
and hops, but lesser reports <strong>for</strong> taste or savour.<br />
Recently transient receptor potential<br />
(TRP) channel family is shown to be involved<br />
in chemical sensation throughout oral cavity.<br />
We wondered if TRP channel agonists would<br />
aff ect <strong>the</strong> fl avour of beer, and screened agonists<br />
from beer and brewing materials. In this<br />
study we will show one of <strong>the</strong> agonists found<br />
in <strong>the</strong> fl avouring material made from heattreated<br />
water extract of hop. This material<br />
contained TRPV1 agonists which are presumed<br />
to be polyphenols and elicit <strong>the</strong> chemical<br />
sensation on <strong>the</strong> tongue. This is <strong>the</strong> fi rst<br />
report that <strong>the</strong> components derived from hop<br />
activates TRPV1 channel. We think this method<br />
is very useful to discover novel fl avour<br />
compounds which could be used in controlling<br />
and improving quality of beer.<br />
Chizuru Sato<br />
Chizuru Sato received a master‘s degree from<br />
<strong>the</strong> Graduate School of Agriculture, Hokkaido<br />
University in 2010. After that she began her<br />
career as a biochemist in <strong>the</strong> Frontier Laboratories<br />
of Value Creation, SAPPORO BRE-<br />
WERIES LTD.<br />
P 070<br />
Stabilization of beer with hop<br />
by-products<br />
Blanka Kotlíková 1, Lukáš Jelínek 1, Pavel<br />
Dostálek 1<br />
1Institute of Chemical Technology, Biotechnology, Prague,<br />
Czech<br />
DESCRIPTION OF TOPIC:<br />
Polyphenols and proteins react toge<strong>the</strong>r and<br />
create complexes which reduce <strong>the</strong> shelf life<br />
of beer. Production of <strong>the</strong> CO 2 hop extract and<br />
hop pellets (type 45) provide by-products rich<br />
in polyphenols that may be used to initiate <strong>the</strong><br />
<strong>for</strong>mation of colloidal haze earlier in <strong>the</strong> brewing<br />
process. The haze can be removed during<br />
fi ltration resulting in an improved colloidal<br />
stability and extended shelf life. We brewed<br />
three beers: one, <strong>the</strong> control beer, was brewed<br />
in a standard way with hop extract, <strong>the</strong><br />
o<strong>the</strong>rs, <strong>the</strong> test beers, were brewed with hop<br />
pellets and hop by-products. All three beers<br />
were brewed to have identical bitterness and<br />
colour. The content of total polyphenols in <strong>the</strong><br />
test beers was signifi cantly higher than in <strong>the</strong><br />
control beer. In addition, <strong>the</strong> antioxidant capacity<br />
in <strong>the</strong> test beers was also signifi cantly<br />
higher. Thus, <strong>the</strong> addition of hop by-products<br />
can improve <strong>the</strong> colloidal and sensory stability<br />
of beer without negatively infl uencing its<br />
sensory characteristics.<br />
Blanka Kotlíková<br />
Holds a MSc degree in Fermentation Chemistry<br />
and Bioengineering from <strong>the</strong> Institute<br />
of Chemical Technology (ICT) in Prague. Currently<br />
she is involved in Postgraduate (PhD)<br />
studies at <strong>the</strong> Department of Biotechnology<br />
(<strong>for</strong>merly Fermentation Chemistry and Bioengineering)<br />
(ICT). Her research interests are in<br />
<strong>the</strong> area of colloidal stability of beer and its<br />
stabilization.<br />
67 34 TH EBC CONGRESS · LUXEMBOURG 26 – 30 MAY 2013
P 072<br />
Applicability of pectin as a stabilizing<br />
and fi ning agent <strong>for</strong> <strong>the</strong><br />
brewing process<br />
Thomas Kunz 1, Kerstin Rudolph 1, Gunnar<br />
Dingel 1, Frank-Jürgen Methner 1<br />
1Technische Universität Berlin, Department of Biotechnology,<br />
Lab of Brewing Science, Berlin, Germany<br />
DESCRIPTION OF TOPIC:<br />
The interactions between polyphenols and<br />
proteins have been identifi ed as one of <strong>the</strong><br />
main reaction systems <strong>for</strong> haze <strong>for</strong>mation in<br />
beer.<br />
Aim of <strong>the</strong> study was to evaluate <strong>the</strong> suitability<br />
of an aqueous pectin solution of citrate and<br />
potassium metabisulfi te as a stabilizing agent<br />
as described in <strong>the</strong> literature. In comparison<br />
to silica gel and PVPP <strong>the</strong> treatment with<br />
pectin only showed no eff ect on <strong>the</strong> colloidal<br />
stability. The increased haze stability seemed<br />
only based on <strong>the</strong> indirect addition of citrate<br />
and SO 2 .<br />
Fur<strong>the</strong>rmore <strong>the</strong> application of pectin as a fi -<br />
ning agent and <strong>the</strong> infl uence of <strong>the</strong> calcium<br />
content in beer on <strong>the</strong> fi ning eff ect have been<br />
investigated. The fi ning activity of pectin could<br />
be verifi ed and is caused by <strong>the</strong> interaction<br />
between pectin and Ca2+ ions which develop<br />
a network <strong>for</strong>mation that traps and sediments<br />
haze particles and yeast cells.<br />
With <strong>the</strong> proper handling pectin is very suitable<br />
to shorten <strong>the</strong> fi ltration time up to 30%,<br />
and to consequently reduce <strong>the</strong> costs.<br />
Thomas Kunz<br />
After qualifying as a certifi ed technician in preservation<br />
engineering (1991-1993), Thomas<br />
Kunz completed his basic studies in chemistry<br />
at University of Applied Sciences, Isny (1994-<br />
1995) and his basic studies in food chemistry<br />
at Wuppertal University (1995-1998), be<strong>for</strong>e<br />
starting to study food technology at University<br />
of Applied Sciences, Trier (1998-2002). After<br />
graduating, he worked as a chartered engineer<br />
in <strong>the</strong> area of ESR spectroscopy at <strong>the</strong><br />
Institute of Bio Physics at Saarland University<br />
(2002-2004). Since January 2005, he has been<br />
employed as a PhD student at <strong>the</strong> research<br />
institute of brewing sciences, Berlin Institute<br />
of Technology (Technische Universität Berlin).<br />
His main research focus lies in analyzing radical<br />
reaction mechanisms in beer and o<strong>the</strong>r<br />
beverages using ESR spectroscopy.<br />
P 073<br />
Diff erent aliphatic structures are<br />
<strong>the</strong> cause and <strong>the</strong> remedy against<br />
primary gushing<br />
Zahra Shokribousjein1, Ann Philippaerts2, Sylvie M. Deckers1, Kurt Gebruers3, Guy S.<br />
Derdelinckx1, Jean Titze4, Vladimir Illberg5 1KULeuven, FBIW-M²S-MbS-LIBR/site Arenberg 33, Leuven<br />
(Heverlee), Belgium, 2KULeuven, FBIW-M²S-COK, Leuven<br />
(Heverlee), Belgium, 3KULeuven, FBIW-M²S-CLMT-LFoRCe,<br />
Leuven (Heverlee), Belgium, 4University College Cork, Cork,<br />
Ireland, 5University of Applied Science Weihenstephan-<br />
Triesdorf, Weihenstephan, Germany<br />
DESCRIPTION OF TOPIC:<br />
Primary gushing of beer is due to <strong>the</strong> explosion<br />
of pressurized nanobubbles <strong>for</strong>med by<br />
<strong>the</strong> association of Class 2 hydrophobins (HFB)<br />
around non dissolved CO 2. It´s typical of hydrophilic<br />
containers. Once this structure is <strong>for</strong>med,<br />
it´s impossible to destroy, except temporarily,<br />
by a physical treatment (<strong>the</strong>rmic or<br />
ultrasonic). Never<strong>the</strong>less, after a period, <strong>the</strong><br />
nanobubbles rebuild as described in <strong>the</strong> model<br />
proposed by Deckers et al (2012). In fact,<br />
<strong>the</strong> affi nity between <strong>the</strong> hydrophobic patch of<br />
HFB and gaseous CO 2 is so high that is impossible<br />
to dissociate <strong>the</strong> structure. It means that<br />
HFB need to be associate with hydrophobic<br />
structures be<strong>for</strong>e <strong>the</strong>y are in contact with CO 2.<br />
Due to <strong>the</strong> low concentrations of HFB involved<br />
(as low as 3 microg/L) peculiar aliphatic compounds<br />
and techniques of food applications<br />
and surface chemistry (magnets) were used<br />
in order to improve <strong>the</strong> specifi city and place<br />
of <strong>the</strong> tratment and <strong>the</strong> dispersion rate of <strong>the</strong><br />
molecule added. Industrial upscaling is now<br />
going on.<br />
Zahra Shokribousjein<br />
Z.Shokribousjein (1978), M.Sc. studied Food<br />
Sciences at <strong>the</strong> Isfahan Universy of Technology,<br />
(Iran). She joined <strong>the</strong> ‚Primary gushing<br />
unit‘ - KULeuven in <strong>the</strong> mid of 2010 and got a<br />
grant of KULeuven-Hydrophobin Chair. Her topic<br />
focuses on exact research in order to understand<br />
<strong>the</strong> specifi c physical and chemical<br />
aspects of <strong>the</strong> mechanisms that cure primary<br />
gushing of beer. She published already two<br />
full-papers and participated actively to EBC<br />
Symposium ‚From Chiller to Filler‘ (Copenhagen-2012).<br />
68<br />
P 074<br />
Critical view on <strong>the</strong> calculation of<br />
pasteurization units in beer<br />
Knut Schwarzer 1, Anna Dammann 1, Ulrich<br />
Müller 1, Jan Schneider 1<br />
1University of Applied Sciences Ostwestfalen-Lippe, Institute<br />
of Live Science Technologies NRW, Lemgo, Germany<br />
DESCRIPTION OF TOPIC:<br />
Beer pasteurization requires a close control of<br />
<strong>the</strong> applicated heat. This is still usually measured<br />
as Pasteurisation units (PU) calculated<br />
by <strong>the</strong> so called beer <strong>for</strong>mula: PU=t*1,393(-<br />
60). This <strong>for</strong>mula derives from investigations<br />
done by Del Veccio et al. in <strong>the</strong> 1950th. Nowadays<br />
energy cost and new sensitive products<br />
require more precise calculations. Several<br />
investigations were made to determine more<br />
precisely key values. The Lemgo D- and zvalue<br />
Database <strong>for</strong> Food collects this data to<br />
simplify its access.<br />
In this work <strong>the</strong> pasteurization key values of<br />
beer spoiling microorganisms with <strong>the</strong> parameter<br />
of <strong>the</strong> beer <strong>for</strong>mula were compared.<br />
Most analyzed species are more heat sensitive<br />
than assumed in <strong>the</strong> beer <strong>for</strong>mula. But<br />
few survive longer up to <strong>the</strong> factor of 10. Still<br />
some beer spoiling species are missing. Here<br />
some additional measuring was done. Remarkable<br />
is that <strong>the</strong> z-value, assumed fi x as<br />
7°C, diff er from 2.7 to 15.4°C with extensive<br />
eff ects at high pasteurization temperatures.<br />
Knut Schwarzer<br />
Dipl. Biol. Knut Schwarzer studdied at <strong>the</strong><br />
Philipps-Universität Marburg (1987 - 1995).<br />
Diploma <strong>the</strong>sis at <strong>the</strong> Kerckhoff -Institut, Max-<br />
Planck-Institute für Herz- und Lungen<strong>for</strong>schung<br />
(1995 - 1996). Training <strong>for</strong> computer<br />
scientist specialization multimedia (2001<br />
- 2003). Research at <strong>the</strong> Universitätsmedizin<br />
Göttingen, Department of Bioin<strong>for</strong>matics<br />
(2003-2006). Research at <strong>the</strong> University of<br />
Applied Sciences Ostwestfalen-Lippe (since<br />
2007).
P 074a<br />
Validation possibilities of cleaning<br />
and disinfection (C&D) foam used<br />
<strong>for</strong> open point cleaning (OPC) of<br />
beverage fi lling lines<br />
Alexander Würtz 1, Phillip Röbisch 2,<br />
Christopher Nüter 1, Roland Folz 1<br />
1VLB Berlin, BBSA, Berlin, Germany, 2University of Applied<br />
Sciences Zittau-Görlitz, Zittau, Germany<br />
DESCRIPTION OF TOPIC:<br />
The evaluation of an appropriate quality of<br />
cleaning foam in <strong>the</strong> brewing and food industry<br />
does depend widely on <strong>the</strong> individual experience<br />
and estimation of <strong>the</strong> user.<br />
Our fundamental challenge in this project is to<br />
develop objective evaluation criteria, assuring<br />
microbiological security at <strong>the</strong> same time. We<br />
describe an approach to a reliable and reproducible<br />
evaluation of OPC at <strong>the</strong> fi lling line.<br />
The development of suitable standard contaminations<br />
(malt syrup, glucose/protein<br />
solution, B. subtilis spore suspension) led to<br />
comparable foam specifi cations; we could generate<br />
<strong>the</strong> following data:<br />
• Run-off speed<br />
• Disinfection power<br />
• Capacity to remove material from surfaces<br />
The results are correlated with diff erent foam<br />
properties and <strong>for</strong>m <strong>the</strong> backbone of this evaluation.<br />
Our current research focuses are:<br />
• Comparing and evaluating foam thickness<br />
• Optical detection possibilities to determine<br />
suffi cient degree of C&D<br />
The presented results are part of two AiF ZIM<br />
coorporation projects.<br />
Alexander Würtz<br />
Born in 1976 Dr. Würtz studied Biochemistry<br />
at <strong>the</strong> Freie Universität Berlin. He got his diploma<br />
degree in 2002 and started after that his<br />
doctoral <strong>the</strong>sis under <strong>the</strong> supervision of Prof.<br />
Dr. Tressl at <strong>the</strong> Technical University Berlin.<br />
After various professional ocupations in federal<br />
German instituts he started as a scientifi c<br />
assistent in <strong>the</strong> VLB in 2012.<br />
P 075<br />
Three-dimensional structural<br />
analysis of kieselguhr particles and<br />
fi lter cakes<br />
Katrin Mathmann 1, Jonathan Schock 2, Julia<br />
Herzen 2, Franz Pfeiff er 2, Heiko Briesen 1<br />
1Technische Universität München, Chair <strong>for</strong> Process Systems<br />
Engineering, Freising, Germany, 2Technische Universität<br />
München, Physics Department (E17), Garching, Germany<br />
DESCRIPTION OF TOPIC:<br />
Precoat fi ltration with kieselguhr is still <strong>the</strong><br />
most frequently used method <strong>for</strong> <strong>the</strong> clarifi -<br />
cation of beer. The process depends strongly<br />
on a large variety of variables. T<strong>here</strong><strong>for</strong>e, <strong>the</strong><br />
prediction of a particular fi ltration run is very<br />
diffi cult. In order to understand <strong>the</strong> fi ltercake<br />
<strong>for</strong>mation and enhance predictability at<br />
<strong>the</strong> same time, two aspects must be considered.<br />
These are <strong>the</strong> characterization of <strong>the</strong><br />
kieselguhr particles and <strong>the</strong> structure of <strong>the</strong><br />
fi lter cake. A commercially available kieselguhr<br />
was characterized by means of diff erent<br />
microscopic techniques and micro-computed<br />
tomography. Against common expectation it<br />
was observed that most particles are not plate<br />
or needle shaped but show a quite regular<br />
spherical shape. The micro-computed tomography<br />
additionally allows <strong>the</strong> three-dimensional<br />
visualization of <strong>the</strong> fi lter-cake structure.<br />
The goal of this study is to support brewers by<br />
regulating <strong>the</strong> fi ltration process on <strong>the</strong> basis<br />
of intrinsic process knowledge.<br />
Katrin Mathmann<br />
Katrin Mathmann graduated in 2005 as an<br />
engineer <strong>for</strong> brewing science and beverage<br />
technology at Weihenstephan. Afterwards,<br />
she researched models <strong>for</strong> describing intraoral<br />
fl uid mechanics under <strong>the</strong> supervision of<br />
Prof. Antonio Delgado, graduating with a PhD<br />
in 2011. Currently, she works as a research<br />
associate at Prof. Heiko Briesen‘s Chair <strong>for</strong><br />
Process Systems Engineering. Her main research<br />
focus is on structural analysis in <strong>the</strong><br />
fi elds of brewing and food science.<br />
P 076<br />
Impact of kieselguhr-free beer fi ltration<br />
on beer (fl avour) stability - a<br />
fi eld report<br />
Beatus Schehl 3, JianJun Zhang 2, Thomas H<br />
Zimmermann 1, Joerg Kress 3, Huiping Li 4<br />
1BASF South East Asia Pte Ltd, Nutrition & Health, Singapore,<br />
Singapore, 2BASF (China) Ltd, Shanghai, China, 3BASF SE,<br />
Lamper<strong>the</strong>im, Germany, 4Guangzhou Zhujiang <strong>Brewery</strong> Co.<br />
Ltd, Guangzhou, China<br />
DESCRIPTION OF TOPIC:<br />
The authors will provide an update of <strong>the</strong> Chinese<br />
Brewing Industry and its consumption<br />
of disposable fi ltration and stabilization aids.<br />
The industrial implementation of kieselguhrfree<br />
precoat-fi ltration and stabilization at Chinese<br />
brewery Guangzhou Zhujiang <strong>Brewery</strong><br />
(Nanning Plant) will be discussed.<br />
To underpin <strong>the</strong> economical and sustainable<br />
eff ect of this regenerative fi lter and stabilizing<br />
aid, results of an Eco-Effi ciency Analysis,<br />
meanwhile evaluated and approved by China<br />
National Light Industry Council, will partially<br />
be highlighted. Next authors will focus on overall<br />
impact on beer stability based on chemical<br />
as well as sensory checks. Data of internal<br />
as well as external parties will be interpreted.<br />
A fur<strong>the</strong>r look into metal absorption from<br />
normally used kieselguhr vs. kieselguhr-free<br />
beer fi ltration will be discussed; fur<strong>the</strong>rmore<br />
eff ect of residual metals such as iron will be<br />
introduced and based on fi eld report positive<br />
impact on beer fl avour stability will be presented.<br />
Beatus Schehl<br />
After <strong>the</strong> studies of food and beverage technology,<br />
Dr. Schehl has worked several years<br />
in brewing and fl avour industry. Since 2011<br />
he is in charge of BASF´s global processing<br />
polymer business <strong>for</strong> beverages. Herein one<br />
of his main focusses lies on innovative and<br />
sustainable technologies in beer and wine<br />
fi ltraion.<br />
69 34 TH EBC CONGRESS · LUXEMBOURG 26 – 30 MAY 2013
P 077<br />
Fine grinding and air classifi cation<br />
of barley malt<br />
Christian Nied 1, Christian Esefeld 2, Petra<br />
Först 1, Karl Sommer 1<br />
1Technische Universität München, Chair of Process Engineering<br />
of Disperse Systems, Freising, Germany, 2Technische<br />
Universität München, Freising, Germany<br />
DESCRIPTION OF TOPIC:<br />
Fine grinding of materials leads to signifi cant<br />
changes of product properties, such as bulk<br />
density and <strong>the</strong> fl ow properties. An increase<br />
of <strong>the</strong> specifi c surface due to grinding also<br />
increases <strong>the</strong> reaction rate as well as dissolution<br />
processes. As could be shown by Tippmann<br />
<strong>for</strong> example, a fi ne milling of barley<br />
malt results in slightly higher brewing yields.<br />
Since fi ne grinding in rotor impact mills causes<br />
a signifi cant rise of <strong>the</strong> product temperature,<br />
a spiral jet mill was chosen <strong>for</strong> comminution.<br />
The barley malt was ground down to a<br />
particle size x99,3 ≈ 50 μm and subsequently<br />
separated into a coarse and a fi ne fraction<br />
using an air classifi er. Due to classifi cation, a<br />
protein rich fraction (also suspected to be an<br />
enzyme rich fraction) and a starch rich fraction<br />
could be obtained as <strong>the</strong> starch granules<br />
were not broken down. Using this grinding<br />
and classifi cation process, developments of<br />
new customized mashing technologies as<br />
well as <strong>for</strong> example high-fi bre beverages<br />
should be possible.<br />
Christian Nied<br />
Christian Nied graduated from Technische<br />
Universität München in 2010 as a degreed<br />
engineer <strong>for</strong> Technology and Biotechnology<br />
of Food. He started his work at <strong>the</strong> Chair of<br />
Process Engineering of Disperse Systems,<br />
TU München as a research associate subsequently.<br />
His current fi elds of activity are <strong>the</strong><br />
physical phenomena during pneumatic dense<br />
phase conveying as well as wet and dry fi ne<br />
grinding.<br />
P 078<br />
The „trickle-down eff ect“ in fl ash<br />
pasteurization: A hydraulic problem<br />
and microbiological risk<br />
Rudolf Michel1, Kristina Böe2 1GEA <strong>Brewery</strong> Systems GmbH, Technology, Kitzingen,<br />
Germany, 2GEA <strong>Brewery</strong> Systems GmbH, Büchen, Germany<br />
DESCRIPTION OF TOPIC:<br />
During <strong>the</strong> fl ash pasteurization of carbonated<br />
beverages, <strong>the</strong> desired heat treatment is<br />
achieved by applying a certain temperature in<br />
a defi ned time interval. The retention time is<br />
determined by <strong>the</strong> length of <strong>the</strong> temperatureholding<br />
tube. The hydraulic design of <strong>the</strong> units<br />
must aim at <strong>the</strong> prevention of <strong>the</strong> release of<br />
carbon dioxide from <strong>the</strong> beverage under all<br />
operating conditions in “hot” zones. The existing<br />
tables and calcuations <strong>for</strong> <strong>the</strong> equilibrium<br />
of CO 2 in beer are extended and used <strong>for</strong> <strong>the</strong><br />
analysis.<br />
If in this system, <strong>the</strong> actual system pressure<br />
falls below <strong>the</strong> saturation pressure in <strong>the</strong><br />
liquid, dissolved carbon dioxide is released<br />
from <strong>the</strong> beverage. Risk areas w<strong>here</strong> <strong>the</strong><br />
pressure can fall below <strong>the</strong> saturation pressure<br />
are identifi ed and discussed. Hydraulic<br />
analysis using CFD are presented.<br />
Finally, <strong>the</strong> consequences <strong>for</strong> <strong>the</strong> process engineering<br />
design of a fl ash pasteurizer system<br />
are discussed and it is shown that this<br />
risk can be minimized with <strong>the</strong> ECO-FLASH<br />
system.<br />
Rudolf Michel<br />
Rudolf Michel was educated at <strong>the</strong> TU München<br />
at Weihenstephan. He was a member of<br />
<strong>the</strong> scientifi c staff at <strong>the</strong> Institute of Chemical<br />
Engineering at Weihenstephan working<br />
on <strong>the</strong> mechanisms of hot break separation<br />
in a whirlpool and on <strong>the</strong> hygienic design of<br />
armatures and pipe work systems. He joint<br />
Huppmann GmbH in 2000. After being taken<br />
over from GEA in 2009 <strong>the</strong> new entity GEA<br />
<strong>Brewery</strong> Systems was built. Currently he his<br />
leading <strong>the</strong> R&D team focusing new process<br />
technologies and sustainablilty.<br />
70<br />
P 079<br />
Effi ciency of dry hopping procedure<br />
<strong>for</strong> improving <strong>the</strong> antioxidant<br />
activity of beer. Comparison with<br />
traditional hop boiling<br />
Marie-Lucie Kankolongo Cibaka 1, Cédric De<br />
taeye 1, Sonia Collin 1<br />
1Université catholique de Louvain, Unité de brasserie et<br />
des industries alimentaires, Earth and Life Institute ELIM,<br />
Louvain-la-Neuve, Belgium<br />
DESCRIPTION OF TOPIC:<br />
Very few is still known concerning <strong>the</strong> structural<br />
evolution of polyphenols from hop to<br />
beer. As shown by HPLC-MS/MS, <strong>the</strong> boiling<br />
step induces procyanidin epimerisation, depolymerisation<br />
and chemical polymerisation<br />
while, in <strong>the</strong> case of dry hopping, ethanol<br />
is able to improve <strong>the</strong> extraction effi ciency.<br />
This procedure also prevents degradation of<br />
heat-sensitive molecules like resveratrol and<br />
piceid. The investigation of wort and beer model<br />
media spiked with commercial fl avan-3-ol<br />
dimers and trimers lead us to identify new<br />
<strong>the</strong>rmically produced oligomeric structures.<br />
An HPLC-online antioxydant assay evidenced<br />
<strong>for</strong> some of <strong>the</strong>m a very high antioxidant activity.<br />
These oligomers diff er from <strong>the</strong> natural<br />
ones by <strong>the</strong> position of <strong>the</strong> interfl avan link,<br />
which is a C2‘-C8 instead of <strong>the</strong> traditional<br />
C4-C8 linkage. Diff erent ways to optimize dry<br />
hopping will be discussed.<br />
Marie-Lucie Kankolongo Cibaka<br />
Graduated in 2012 as Bio-Engineer from<br />
Université catholique de Louvain, Belgium.<br />
She carried out her master degree in Food<br />
Science, Quality and Technology, at <strong>the</strong> Earth<br />
and Life Institute. After graduation, she started<br />
a Ph.D. program at this institute. Her work<br />
is focused on new potentialities of thiol-rich<br />
hop cultivars <strong>for</strong> dry-hopping and bottle refermentation.
P 080<br />
Use of aroma condensates of brewery<br />
fermentation gas <strong>for</strong> organoleptic<br />
optimization of alcohol-free<br />
beers<br />
Thomas Tyrell 1, Thomas Gieche 1, Jan<br />
Biering 1, Christof Loch 2, Hans-Peter Bach 3,<br />
Patricia Diniz 1, Alexander Würtz 1, Roland<br />
Folz 1<br />
1VLB Berlin, Berlin, Germany, 2Clemens GmbH & Co. KG,<br />
Wittlich, Germany, 3priv, Trier, Germany<br />
DESCRIPTION OF TOPIC:<br />
Besides missing alcohol fl avour active components<br />
such as esters, higher alcohols and<br />
aldehydes are driven out by de-alcoholization<br />
process or are not <strong>for</strong>med during an „incomplete“<br />
fermentation in non-alcoholic beer production.<br />
This is seen as a reason <strong>for</strong> strong<br />
fl avour diff erence between non-alcoholic and<br />
normal beers. In <strong>the</strong> described research project<br />
a two stage cooling unit was placed into<br />
fermentation gas stream in order to gain condensate<br />
rich in volatile substances of beer.<br />
Condensates were blended into non-alcoholic<br />
beers and changes in sensorial characteristics<br />
had been evaluated. Trials were run<br />
in 4 diff erent breweries in order to evaluate<br />
condensates of special beer fermentations of<br />
top and bottom fermenting yeasts. The poster<br />
presents results of sensorial and analytical<br />
evaluations of condensates and resulting<br />
beers. Additionally a description of aroma<br />
condensate yield is given and a discussion<br />
of <strong>the</strong> potential of brewery fermentaiton gas<br />
aroma is done.<br />
Thomas Tyrell<br />
Thomas Tyrell is an apprenticed and experienced<br />
brewer and maltster starting in 1991.<br />
Studying in Berlin at <strong>the</strong> Technical University<br />
from 2001-2006 he received a “Diplom<br />
Ingenieur“ degree in brewing science. Since<br />
early 2007 he is at VLB and since 2008 part<br />
of <strong>the</strong> VLB department: Brewing and Beverage<br />
Science & Applications At present he is<br />
responsible <strong>for</strong> applied research/consulting<br />
projects. Additionally coordination of institutes<br />
activities <strong>for</strong> Iberoamérica belongs to his<br />
responsibilities.<br />
P 081<br />
New method <strong>for</strong> de-alcoholization<br />
of beer<br />
Michael Dillenburger 1, Katja Hertel 1<br />
1Dillenburger & Hertel GmbH, Freising, Germany<br />
DESCRIPTION OF TOPIC:<br />
Non-alcoholic beer is gaining more and more<br />
importance. In a gobalized world with its requirements<br />
in mobility it is a welcome alternative.<br />
Due to its isotonic characteristics it is<br />
ideal <strong>for</strong> sportspeople. And it adds to people´s<br />
health and responsibility. Thus non-alcoholic<br />
beer show a considerable increase of market<br />
share.<br />
However, state-of-<strong>the</strong> art methods <strong>for</strong> its production<br />
show various disadvantages such as<br />
high investment and high operating costs accompanied<br />
by sensory deviations by <strong>the</strong>rmal<br />
impact etc.<br />
A new method based on desorption allows<br />
to produce non-alcoholic beer by using inert<br />
gas or CO 2. All media and equipment is already<br />
provided by a brewery leeding to low<br />
investment and operating cost. Fur<strong>the</strong>rmore<br />
it will be able to selectively remove just ethanol.<br />
Thus <strong>the</strong> sensory characteristics will be<br />
(ra<strong>the</strong>r) <strong>the</strong> same as with <strong>the</strong> conventional<br />
beer. In <strong>the</strong> last step of this innovation it will<br />
be possible to have a de-alcoholization right<br />
in place at <strong>the</strong> dispending equipment.<br />
Michael Dillenburger<br />
Dipl.-Ing. Michael Dillenburger was born in<br />
1977 in Trier, Germany. After his studies in<br />
2004 he fi rst stayed with <strong>the</strong> Technische Universität<br />
München, Weihenstephan. Michael<br />
Dillenburger has <strong>the</strong>n been production manager<br />
of a considerable distillery be<strong>for</strong>e working<br />
as executive assistant with a patent and law<br />
fi rm in Freising. Having been Sales Manager<br />
with HERTEL GmbH <strong>for</strong> 4 years he is now responsible<br />
<strong>for</strong> <strong>the</strong> technical concerns of DIL-<br />
LENBURGER & HERTEL GmbH as CEO<br />
P 082<br />
MEURASTREAM: A global concept<br />
of wort production with reduced<br />
consumption of energy and process<br />
water<br />
Ariane Dewulf 1, Frédérique Harmegnies 1,<br />
Pascal Cantillon 2<br />
1Meura sa, Research and Development, Louvain-la-Neuve,<br />
Belgium, 2Meura sa, Péruwelz, Belgium<br />
DESCRIPTION OF TOPIC:<br />
Wort production in breweries requires consecutive<br />
heating and cooling steps consuming<br />
energy and water. For years most studies have<br />
focused on wort boiling which has <strong>the</strong> highest<br />
energy demand of <strong>the</strong> brewing process.<br />
Developed systems, as <strong>the</strong> “Pfaduko” using a<br />
hot liquor storage tank, allow reducing energy<br />
consumptions but do not solve <strong>the</strong> hot water<br />
excess.<br />
This poster presents a global concept of wort<br />
production with a de-intensifi ed wort boiling<br />
process including <strong>for</strong>mation/stripping technologies<br />
as well as an innovative energy recovery<br />
system called <strong>the</strong> MEURASTREAM.<br />
Its principle diff ers from o<strong>the</strong>rs by <strong>the</strong> use of<br />
process water at counter fl ow within <strong>the</strong> whole<br />
brewing process.<br />
The MEURASTREAM allows energy management<br />
optimization of <strong>the</strong> wort production process<br />
whilst reducing hot water surplus.<br />
Also this publication compares fi gures of<br />
energy and water consumptions with existing<br />
systems, as well as cold wort qualitative results<br />
from pilot trials.<br />
Ariane Dewulf<br />
Ariane Dewulf was graduated in 2007 as a<br />
Brewing Engineer from <strong>the</strong> Catholic University<br />
of Louvain-La-Neuve in Belgium. For her<br />
dissertation, she worked in <strong>the</strong> laboratory of<br />
Professor Meurens in collaboration with <strong>the</strong><br />
Roche<strong>for</strong>t brewery on <strong>the</strong> topic of: “Follow up<br />
of beer fermentation and refermentation by<br />
near infrared spectroscopy“. Since 2007, she<br />
has been working <strong>for</strong> Meura as a Research<br />
and Development Engineer.<br />
71 34 TH EBC CONGRESS · LUXEMBOURG 26 – 30 MAY 2013
P 083<br />
Making gluten-free beers with<br />
barley malt and a proline-specifi c<br />
endoprotease<br />
Sylvie M Van Zandycke 1, Chantal Christis 2,<br />
Luppo Edens 2, Fredoen Valianpour 2, Joze<br />
Mutsaers 2, Ron Duszanskyj 2<br />
1DSM Food Specialties, Enzyme Solutions, South Bend,<br />
United States, 2DSM Food Specialties, Delft, The Ne<strong>the</strong>rlands<br />
DESCRIPTION OF TOPIC:<br />
Gluten free beers are generally made with<br />
grains that do not contain gluten such as sorghum,<br />
rice, buckwheat… These beers are safe<br />
to drink <strong>for</strong> people who display sensitivities<br />
to gluten or have been diagnosed as having<br />
celiac disease; a potential life-threa<strong>the</strong>ning<br />
condition. It is also possible to produce beers<br />
that are low in gluten when grains containing<br />
gluten are used. Indeed, a large amount of<br />
gluten proteins are removed during <strong>the</strong> brewing<br />
process and additionally, a proline-specifi<br />
c endoprotease (PSEP) has been suggested<br />
as a mean to hydrolyze <strong>the</strong> remaining gluten<br />
proteins from barley malt to bring <strong>the</strong> levels<br />
down to undetectable (results vary according<br />
to dosage of <strong>the</strong> enzyme and method used to<br />
measure gluten). This paper will focus on <strong>the</strong><br />
nature of PSEP and <strong>the</strong> process of making<br />
gluten-free beers with malting barley including<br />
<strong>the</strong> recommended procedures to measure<br />
gluten accurately and current levels of<br />
gluten in commercial beers.<br />
Sylvie M Van Zandycke<br />
Sylvie studied biochemical engineering at <strong>the</strong><br />
Institute Meurice (Brussels, Belgium). She<br />
<strong>the</strong>n obtained her Ph.D. in 2000 from Ox<strong>for</strong>d<br />
Brookes University in <strong>the</strong> UK. After that Sylvie<br />
was employed as Project Manager <strong>for</strong> SMART<br />
Brewing Services <strong>the</strong>n in 2004 Project Manager<br />
<strong>for</strong> <strong>the</strong> Lallemand Genetic ID Lab in Montreal,<br />
Canada <strong>the</strong>n in 2007 Technical Sales<br />
Manager <strong>for</strong> Lallemand Brewing. At <strong>the</strong> end<br />
of 2011 she joined DSM Food Specialties as<br />
Technical Service Manager <strong>for</strong> brewing enzymes<br />
in North America.<br />
P 084<br />
Potential of aroma-modifi ed<br />
distiller‘s grains <strong>for</strong> <strong>the</strong> application<br />
in cereal based functional beverages<br />
Magdalena Rückert1, Martin Zarnkow1, Thomas Becker1 1Technische Universität München, Center of Life Sciences<br />
Weihenstephan, Chair of Brewing and Beverage Technology,<br />
Freising, Germany<br />
DESCRIPTION OF TOPIC:<br />
Distiller‘s grains (DG), <strong>the</strong> cereal by-product<br />
coming up during <strong>the</strong> production process<br />
of fuel or beverage alcohol, are prevalently<br />
used as feed additives and material <strong>for</strong> biogas<br />
plants. DG contain all ingredients of <strong>the</strong><br />
whole grain in a concentrated <strong>for</strong>m, especially<br />
high amounts of protein and dietary fi ber. The<br />
nutritional properties of DG provide a new interest<br />
in using DG as a food ingredient and as<br />
a substrate <strong>for</strong> cereal based functional beverages.<br />
One important reason <strong>for</strong> not using DG as a<br />
food ingredient yet is <strong>the</strong> characteristic fl avor.<br />
Our aim is to analyze <strong>the</strong> aroma profi le<br />
of DG from wheat and neutralize reasonable<br />
components <strong>for</strong> getting a modifi ed proper fl avor.<br />
T<strong>here</strong><strong>for</strong>e <strong>the</strong> aroma of DG was defi ned<br />
trough sensorial analysis and aroma active<br />
components were analyzed with GC-MS. Extracts<br />
of DG <strong>the</strong>n were treated with charcoal<br />
and assessed by sensorial analysis.<br />
Magdalena Rückert<br />
Magdalena Rückert was born in 1985 in Poland.<br />
She graduated 2009 with a state examination<br />
in Food Chemistry at <strong>the</strong> Friedrich-<br />
Alexander University Erlangen-Nürnberg and<br />
2011 as a state certifi ed Food Chemist at <strong>the</strong><br />
Bavarian Health and Food Safety Authority.<br />
Since 2011 she works as a PhD student at <strong>the</strong><br />
Chair of Brewing and Beverage Technology at<br />
Technische Universität München, in <strong>the</strong> fi eld<br />
of new functional beverages and fl avour research.<br />
72<br />
P 085<br />
Simulation of mashing process<br />
Simon Weber 1, Heiko Briesen 1<br />
1Technische Universität München, Chair <strong>for</strong> Process Systems<br />
Engineering, Freising, Germany<br />
DESCRIPTION OF TOPIC:<br />
Starch hydrolysis by amylases is strongly<br />
infl uenced by <strong>the</strong> starch polymer structure<br />
(chain lengths and branches). Amylases binding<br />
<strong>the</strong> starch polymer have several subsites<br />
located around <strong>the</strong> active centre of <strong>the</strong> enzymes.<br />
T<strong>here</strong> exist several possibilities of polymer<br />
binding with diff erent resulting binding<br />
energies (BE). With BE and <strong>the</strong> enzyme activities<br />
(EA) it is possible to calculate <strong>the</strong> resulting<br />
chain length distributions and hydrolysis<br />
rates. In this work we develop new models<br />
based on ordinary diff erential equations (ODE,<br />
fast but infl exible) and kinetic Monte-Carlo<br />
technique (kinMC, slow but fl exible). The two<br />
approaches use a consistent set of parameters<br />
(BE and EA) and enable an effi cient combination<br />
of both techniques <strong>for</strong> simulation of<br />
starch hydrolysis. The benefi ts <strong>for</strong> brewing<br />
are <strong>the</strong> optimization of mashing process with<br />
a well-defi ned chain length distribution (constant<br />
product quality or new innovative beers),<br />
even if <strong>the</strong> starch source will be changed.<br />
Simon Weber<br />
As of 2011 - Dissertation: ‚Simulation of<br />
Mashing Process‘ 2011 - Dipl.-Ing. Biosystem<br />
engineering, Otto-von-Guericke-Universität<br />
Magdeburg
P 086<br />
Investigation of a new prototype<br />
mashing system<br />
Tim J Hobley 1, Daniel Christiansen 1, Thomas<br />
Krüger 1, Frederik Wilbek 1, Nikolaj Hansen 1,<br />
Anders Nielsen 1, Peter Stubbe 1, Preben<br />
Hansen 1<br />
1Technical University Denmark, Institute <strong>for</strong> Food, Lyngby,<br />
Denmark<br />
DESCRIPTION OF TOPIC:<br />
High yields and wort quality still concerns<br />
small and large breweries, especially when<br />
using raw barley and added enzymes. Conventional<br />
processes are not optimal <strong>for</strong> use of<br />
added enzymes due to poor mixing or deactivation<br />
with extreme temperature. Circulation<br />
of wort in alternating directions during<br />
mashing in a kettle with fi lter meshes at <strong>the</strong><br />
top and bottom is eff ective up to 2 hL. But high<br />
pressure on <strong>the</strong> fi lter end plates limits scaleup.<br />
Here, we present a new mashing kettle<br />
which eliminates fi lter plates, but allows circulation<br />
and heating of <strong>the</strong> wort, entrapment<br />
of particles, rapid draining and sparging. Per<strong>for</strong>mance<br />
of <strong>the</strong> prototype, showed <strong>the</strong> temperature<br />
profi le was highly reproducible. A<br />
mixing time of ca. 90 seconds was seen using<br />
a fl ow rate of 6 L/min through <strong>the</strong> external<br />
loop. Mashing of 2.5 kg milled barley, 8 kg<br />
water (pH 5.7), 6.25 g OndeaPro (Novozymes)<br />
with a profi le of 10 min at 53°C, 60 min at<br />
65°C and 5 min at 78°C, yielded 13.5°Brix.<br />
Tim J Hobley<br />
Ph.D. Chemical Engineering, University of<br />
Melbourne, Australia, followed by postdoc<br />
in Lund, Sweden in Technical Microbiology.<br />
Subsequently employed at DTU Denmark as<br />
Postdoc, Assistant Professor and Associate<br />
professor in department of Systems Biology,<br />
in <strong>the</strong> areas of fermentation and downstream<br />
processing. Now at National Institute of Food<br />
at DTU as associate professor with responsibility<br />
<strong>for</strong> micro brewery and fermentation and<br />
downstream processing.<br />
P 087<br />
Infl uence of <strong>the</strong> mashing regime<br />
on <strong>the</strong> phenolic character of wheat<br />
beer<br />
Frithjof Thiele 1, Andreas Ludwig 1<br />
1Radeberger Gruppe KG, Frankfurt, Germany<br />
DESCRIPTION OF TOPIC:<br />
Phenolic aroma compounds (e.g. 4-vinylguaiacol<br />
(4-VG) deriving from ferulic acid) are<br />
typical fl avours in wheat beer. Formation and<br />
infl uencing factors in brewing of <strong>the</strong>se compounds<br />
are well known, mashing temperature<br />
and mash-pH were identifi ed as key parameters.<br />
In all published papers each factor was<br />
investigated individually, however, with this<br />
experimental design interactions between<br />
<strong>the</strong> infl uencing factors can not be detected.<br />
In this investigation a response surface methodology<br />
experimental design was employed<br />
to study <strong>the</strong> interactions between <strong>the</strong> main<br />
factors temperature and pH on a small scale<br />
level. Using mashing-in-temperatures from<br />
45°C to 63°C and mash-pH 5.2 to 5.8 it was<br />
possible to show that a temperature increase<br />
lead to a decrease in released ferulic acid. The<br />
eff ect of <strong>the</strong> pH was limited to <strong>the</strong> mashingin-temperatures<br />
around 45°C. Fur<strong>the</strong>rmore<br />
diff erent yeast strains used in large scale<br />
brewing were analysed <strong>for</strong> <strong>the</strong>ir potential to<br />
decarboxylate ferulic acid into 4-VG.<br />
Frithjof Thiele<br />
Frithjof studied Brewing and Beverage Technology<br />
at Technische Universität München-<br />
Weihenstephan from 1996-2002. He worked<br />
as Scientifi c Employee at <strong>the</strong> Institute <strong>for</strong><br />
Brewing Technology I, Technische Universität<br />
München-Weihenstephan from 2002-2007<br />
and received a doctoral degree in 2006. In<br />
2008 he went to Ireland to work as Post-<br />
Doctoral Researcher at <strong>the</strong> University College<br />
Cork. Since 2009 he works <strong>for</strong> <strong>the</strong> German<br />
Brewing Group “Radeberger Gruppe KG“ currently<br />
as Head of Corporate Technology<br />
P 088<br />
Genedata Selector - enterprise<br />
genome management and analysis<br />
software to optimize fermentation<br />
processes<br />
Thomas Hartsch 1<br />
1Genedata AG, Basel, Switzerland<br />
DESCRIPTION OF TOPIC:<br />
Innovative technologies like next generation<br />
sequencing, transcriptomics, proteomics<br />
and metabolomics enable deep insights (e.g.<br />
in yeast physiology) at <strong>the</strong> molecular level.<br />
With this additional knowledge, fermentation<br />
processes can be improved and made more<br />
effi cient and cost-eff ective.<br />
Genedata Selector integrates, analyses and<br />
visualizes all types of experimental data from<br />
genomes, pathways and phenotypes to intellectual<br />
property. We demonstrate how <strong>the</strong><br />
system integrates proprietary and public molecular<br />
data from yeast, microbes, and plants<br />
and has been used successfully in strain optimization<br />
projects, investigation of spoilage<br />
causes and <strong>the</strong> development of process monitoring<br />
tools <strong>for</strong> microbial identity, viability and<br />
stability. Enterprise-level, <strong>the</strong> system streamlines<br />
processes and is accessible across departments<br />
and production sites.<br />
Thomas Hartsch<br />
Dr. Thomas Hartsch studied chemistry and<br />
molecular biology. He joined <strong>the</strong> Göttingen<br />
Genomics Lab‘ management team and gained<br />
his fi rst experience in industry at Integrated<br />
Genomics. Here he was in charge of a high<br />
throughput sequencing lab, data storage and<br />
analysis and customer solutions. Eight years<br />
ago he joined Genedata, w<strong>here</strong> he is managing<br />
<strong>the</strong> business development activities and<br />
scientifi c consulting projects <strong>for</strong> Industrial<br />
Biotechnology, Agribusiness, and <strong>the</strong> Food<br />
and Beverage Industry.<br />
73 34 TH EBC CONGRESS · LUXEMBOURG 26 – 30 MAY 2013
P 089<br />
Process Analytical Technology – a<br />
paradigm change of systematic<br />
process evaluation and product<br />
release<br />
Stephan Birle 1, M.A. Hussein 1,<br />
Thomas Becker 1<br />
1Technische Universität München (TUM), Freising, Germany<br />
DESCRIPTION OF TOPIC:<br />
Fermentative processes contain living organisms<br />
and t<strong>here</strong><strong>for</strong>e are subjected to dynamic,<br />
nonlinear and time-variant process behavior.<br />
As initial conditions usually cannot be<br />
kept constant <strong>the</strong>y put a great challenges with<br />
respect to process monitoring and control.<br />
The PAT (Process Analytical Technology) initiative<br />
presents an innovative system <strong>for</strong> design,<br />
analysis and control of production via<br />
measurement of critical quality parameters<br />
of raw and process materials with <strong>the</strong> aim<br />
of secured product quality. In contradiction to<br />
conventional product release via lab analysis,<br />
it strives <strong>for</strong> a process-oriented validation<br />
and release of process sections in real-time<br />
and t<strong>here</strong><strong>for</strong>e leads to a novel approach of<br />
process assessment.<br />
This contribution presents a comprehensive<br />
and practical implementation of a PAT strategy<br />
to <strong>the</strong> fermentation and maturation process<br />
of a medium-sized brewery with focus<br />
on applied PAT tools of intelligent process<br />
control, data-mining and pattern recognition.<br />
Stephan Birle<br />
2005-2010: Study Brewing and Beverage<br />
Technology, TU Munich 2010 - now: PhD<br />
student, TU Munich, Chair of brewing and<br />
beverage technology Topic: Fuzzy logic and<br />
intelligent control strategies <strong>for</strong> fermentative<br />
processes<br />
P 090<br />
Pilot VHG brewing: pure oxygen,<br />
yeast preconditions or higher<br />
temperatures?<br />
José António Teixeira3, Luis Lima1, Tiago<br />
Brandão2, Nelson Lima3 1Universidade do Minho, Departamento de Engenharia<br />
Biológica, Braga, Portugal, 2Unicer Bebidas, SA, Leça do Balio,<br />
Portugal, 3Universidade do Minho, Braga, Portugal<br />
DESCRIPTION OF TOPIC:<br />
The use of unsaturated fatty acids to supplement<br />
<strong>the</strong> yeast slurry be<strong>for</strong>e pitching allowed<br />
faster extract reduction and lower fi nal attenuation<br />
than fermentations with 22-24ppm<br />
of initial O 2. The use of <strong>the</strong> nutritive cocktail to<br />
supplement <strong>the</strong> yeast slurry avoided <strong>the</strong> intense<br />
foaming observed during <strong>the</strong> fi rst hours<br />
of 18ºC fermentations. At VHG condition,<br />
diacetyl was reduced below 0.1 mg/L be<strong>for</strong>e<br />
<strong>the</strong> primary fermentation was completed. The<br />
variation of <strong>the</strong> higher alcohols/esters ratio<br />
ranged only between 3.7 and 4.7. The sensorial<br />
triangular test between <strong>the</strong> VHG beers without<br />
nutritional treatment and standard conditions<br />
showed identical aroma profi les. This<br />
is an important indicator of <strong>the</strong> feasability of<br />
VHG brewing at 18ºC to obtain identical profi -<br />
les when compared to 15ºP wort fermented<br />
at 12ºC. The sensorial test indicated that wort<br />
saturation with pure O 2 can be replaced by<br />
yeast treatment with unsaturated fatty acids<br />
without changes in <strong>the</strong> beer aroma profi le<br />
José Teixeira<br />
José Teixeira is Professor of Biological Engineering<br />
at Universidade do Minho. José<br />
Teixeira graduated in Chemical Engineering<br />
at Universidade do Porto, in 1980, <strong>the</strong> same<br />
university w<strong>here</strong> he obainde his PhD in 1988.<br />
His main research interests are bioprocess<br />
development, yeast physiology and food technology.He<br />
was <strong>the</strong> Principal Investigator of 32<br />
research projects and supervisor of 31 PhDs<br />
and 16 pos-doc researchers. He authored/coauthored<br />
over 310 peer-reviewed papers.<br />
74<br />
P 091<br />
Screening of brewing yeast strains<br />
<strong>for</strong> development of probiotic craft<br />
beers<br />
Monique Dillemans 1,<br />
Laurence Van Nedervelde 1<br />
1Institut Meurice, Brewing Department, Brussels, Belgium<br />
DESCRIPTION OF TOPIC:<br />
The aim of this work is to develop new barley<br />
malt functional beverages fermented by<br />
yeasts with probiotic physiological properties.<br />
Indeed, probiotic cultures have been used<br />
as preventive and <strong>the</strong>rapeutic agent <strong>for</strong> <strong>the</strong><br />
treatment of diverse digestive diseases. The<br />
fi rst selection among 25 industrial brewing<br />
yeasts Saccharomyces cerevisia was based<br />
on <strong>the</strong>ir gastrointestinal tract tolerance (acid<br />
and basic pH, presence of bile salts and digestive<br />
enzymes). The most resistant strains<br />
were tested <strong>for</strong> <strong>the</strong>ir ability to ad<strong>here</strong> to human<br />
cells, <strong>the</strong>ir potential <strong>for</strong> induction of proinfl<br />
ammatory cytokine by intestinal cells and<br />
o<strong>the</strong>r criteria relevant <strong>for</strong> any potentially probiotic<br />
microorganism. Two pharmaceutical<br />
probiotic yeast strains, Saccharomyces boulardii<br />
were used as reference. Among brewing<br />
yeasts tested, three strains have proved <strong>the</strong>ir<br />
effi ciency <strong>for</strong> high quality probiotic health<br />
beers production, with acceptable shelf life,<br />
depending on alcohol degree and storage<br />
temperature.<br />
Monique Dillemans<br />
Studies: 1978: Brewing Engineer from <strong>the</strong><br />
Meurice Institute in Brussels. 1981: MSc in<br />
Natural Science from <strong>the</strong> Catholic University<br />
of Louvain 1990: <strong>European</strong> Certifi cate in<br />
dermo-cosmetology at <strong>the</strong> University of Brussels.<br />
Current position: Since 1978 Research<br />
Manager at <strong>the</strong> department of Brewing Sciences<br />
of Meurice Institute. Her main research<br />
activity is concentrated on yeast metabolism<br />
and yeast derivatives (antioxidant, yeast activators...).
P 092<br />
Reactivation of exhausted yeast<br />
physiological activity<br />
Mario Novak 1, Filip Cingesar 1, Zvonko<br />
Petrović 2, Philippe Cario 3, Božidar Šantek 1<br />
1University of Zagreb, Faculty of Food Technology and<br />
Biotechnology, Department of Biochemical Engineering,<br />
Zagreb, Croatia, 2Ireks Aroma d.o.o, Zagreb, Croatia, 3AEB<br />
SPA, Brescia, Italy<br />
DESCRIPTION OF TOPIC:<br />
Aims: The aim of this research was to prolong<br />
<strong>the</strong> yeast re-pitching process without loosing<br />
beer quality.<br />
Methods: In this research lager yeast (5th<br />
generation stored at + 4 oC <strong>for</strong> 5 days) was<br />
used <strong>for</strong> wort fermentation w<strong>here</strong> yeast properties<br />
and metabolites concentrations were<br />
monitored. In order to reactivate yeast activity<br />
diff erent nutrients mixtures (amino-acids,<br />
peptides, proteins, vitamins and minerals)<br />
were prepared.<br />
Results: The use of inorganic or organic nutrients<br />
mixtures insures that fermentation will<br />
be successfully completed compared to <strong>the</strong><br />
fermentation without nutrients mixture. The<br />
use of organic nutrients mixture resulted in<br />
<strong>the</strong> increase of yeast concentration and in <strong>the</strong><br />
reactivation of fermentation process. Reactivated<br />
yeast was also capable to per<strong>for</strong>m a<br />
few more fermentations.<br />
Conclusions: The use of organic nutrients<br />
mixture can signifi cantly improve exhausted<br />
yeast activity and to reactivate declined fermentation<br />
without considerable impact on <strong>the</strong><br />
beer quality.<br />
Mario Novak<br />
Research Assistant / PhD student at University<br />
of Zagreb, Faculty of Food Technology and<br />
Biotechnology, Department of Biochemical<br />
Engineering. Research area is ma<strong>the</strong>matical<br />
modelling and mass transfer research in production<br />
of biofuels and ma<strong>the</strong>matical modelling<br />
of fermentation and yeast metabolism.<br />
P 093<br />
Evaluation of Near-Infrared Spectroscopy<br />
(NIRS) <strong>for</strong> on-line determination<br />
of critical process parameters<br />
<strong>for</strong> fermentation process<br />
control<br />
Lucas Vann 1, Johnathon B Layfi eld 1, John D<br />
Sheppard 1<br />
1North Carolina State University, Food, Bioprocessing & Nutrition<br />
Sciences, Raleigh, United States<br />
DESCRIPTION OF TOPIC:<br />
The search <strong>for</strong> improved methods <strong>for</strong> process<br />
control has led to <strong>the</strong> development and application<br />
of Near-Infrared Spectroscopy (NIRS).<br />
NIRS can be implemented in real-time with<br />
several determinations being made simultaneously<br />
from a single spectral scan t<strong>here</strong>by<br />
maximizing process monitoring and control<br />
capabilities. The present research explores its<br />
potential <strong>for</strong> on-line fermentation monitoring<br />
of specifi c gravity (SG), free amino nitrogen<br />
(FAN) and percent alcohol by volume (% EtOH<br />
v/v) <strong>for</strong> <strong>the</strong> purpose of control of fermentation<br />
rate and maturation time. Pilot-scale batch<br />
model predictions correlated well with off -<br />
line analysis yielding <strong>the</strong> following average<br />
error of predictions: SG (0.09%), EtOH (3.39%),<br />
FAN (4.85%). The fi ndings support <strong>the</strong> possibility<br />
of incorporating NIRS into commercial<br />
brewing operations <strong>for</strong> continuous “real time”<br />
assurance of quality by permiting early fault<br />
detection and <strong>the</strong> real-time control of yeast<br />
metabolism, <strong>for</strong> example, through temperature<br />
adjustment.<br />
Lucas Vann<br />
Currently employed as a Senior Scientist in<br />
<strong>the</strong> Biomanufacturing Training and Education<br />
Center at North Carolina State University and<br />
is pursuing a PhD on <strong>the</strong> use of NIRS <strong>for</strong> <strong>the</strong><br />
control of a variety of cell-culture processes,<br />
as applied to brewing and biopharmaceuticals.<br />
He has a masters degree in Biosytems<br />
Engineering from McGill University in Canada,<br />
w<strong>here</strong> he helped in <strong>the</strong> development of a biosensor<br />
<strong>for</strong> measuring diacetyl concentration<br />
in beer.<br />
P 094<br />
A systematic search <strong>for</strong> novel yeast<br />
strains with superior fermentation<br />
characteristics<br />
Jan Steensels 1, Gino J.E. Baart 1, Tim Snoek 1,<br />
Es<strong>the</strong>r Meersman 1, Veerle Saels 1, Martina<br />
Picca Nicolino 1, Kevin J Verstrepen 1<br />
1KU Leuven, CMPG/VIB, Leuven, Belgium<br />
DESCRIPTION OF TOPIC:<br />
Fermented foods and beverages have been<br />
consumed by humans <strong>for</strong> over 8000 years.<br />
Originally, <strong>the</strong>se fermentations were spontaneous<br />
processes, without any control or<br />
knowledge of <strong>the</strong> microbial driving <strong>for</strong>ce behind<br />
<strong>the</strong>m. To increase reproducability, welldefi<br />
ned starter cultures were introduced.<br />
Until recently, <strong>the</strong> tools and knowledge were<br />
lacking to make a scientifi cally found choice<br />
about which strain to use in <strong>the</strong>se starters. In<br />
this study, we screened a wide variety of industrially<br />
relevant yeast strains. This resulted<br />
in a large collection of yeast strains (>600),<br />
with each strain characterized to an unprecedented<br />
level of detail. Our data allows us to<br />
rapidly select strains <strong>for</strong> specifi c industrial<br />
purposes. Additionally, this dataset provides<br />
an excellent plat<strong>for</strong>m to select strains <strong>for</strong><br />
breeding of novel yeasts with benefi cial traits<br />
from both parents. Using several diff erent<br />
breeding approaches (all non-GMO), hybrids<br />
with superior benefi cial characteristics were<br />
constructed.<br />
Jan Steensels<br />
Jan Steensels received a BSc in Bioscience<br />
Engineering from <strong>the</strong> University of Leuven,<br />
Belgium in 2008 and a MSc in Bioscience<br />
Engineering, major “Cell and Gene technology“,<br />
minor “Industrial Microbiology“ from <strong>the</strong><br />
same university in 2010. He did his master<br />
<strong>the</strong>sis in <strong>the</strong> Centre <strong>for</strong> Malting and Brewing<br />
Science in 2009-2010. In 2010, Jan joined<br />
<strong>the</strong> VIB laboratory <strong>for</strong> Systems Biology led by<br />
prof. Kevin Verstrepen as a PhD student.<br />
75 34 TH EBC CONGRESS · LUXEMBOURG 26 – 30 MAY 2013
P 095<br />
Modeling <strong>the</strong> attenuation of extract<br />
during brewing operations:<br />
tracing <strong>the</strong> black box<br />
R. Alex Speers 1, Andrew J MacIntosh 1<br />
1Dalhousie University, Process Engineering and Applied<br />
Science, Halifax, Canada<br />
DESCRIPTION OF TOPIC:<br />
Industry giants have historically described<br />
<strong>the</strong> process of brewing as a “black box” operation,<br />
however brewers utilize known trends<br />
and rules to describe many fermentation parameters.<br />
For example, extract attenuation<br />
typically follows a sigmoidal decline to which<br />
numerous equations have been fi t allowing<br />
brewers to predict, assess and compare<br />
fermentations. T<strong>here</strong> are several commonly<br />
applied models within <strong>the</strong> brewing industry,<br />
each with advantages and disadvantages.<br />
This paper fi ts and compares several common<br />
sigmoidal models (including <strong>the</strong> logistic,<br />
incomplete beta-function and o<strong>the</strong>rs) to<br />
industrial and lab-scale brewing attenuation<br />
data. The results of this study show that <strong>the</strong><br />
attenuation of brewing fermentations follows<br />
a non-symmetrical sigmoidal distribution and<br />
should be modeled accordingly when suffi cient<br />
data is available. Of <strong>the</strong> models assessed,<br />
a logistic model most accurately fi t <strong>the</strong> data.<br />
The advantages and disadvantages of common<br />
fermentation models are also discussed.<br />
R. Alex Speers<br />
Alex Speers has been appointed Professor,<br />
Chair and Director of <strong>the</strong> International Centre<br />
of Brewing and Distilling at Heriot Watt<br />
University eff ective spring 2013. Until <strong>the</strong>n<br />
he will continue as a professor of Food Science<br />
at Dalhousie University. He was a past<br />
Chair of Editorial Board of <strong>the</strong> MBAA TQ and<br />
is a member of <strong>the</strong> J ASBC, JIB and <strong>the</strong> TQ<br />
editorial boards. He has published or presented<br />
more than 150 papers and is a Fellow of<br />
<strong>the</strong> IBD. In 2011 he was awarded <strong>the</strong> WJ Eva<br />
award by <strong>the</strong> CIFST.<br />
P 096<br />
Optimisation of fermentation<br />
cooling profi le<br />
Elaine Martin 1,2, Elaine B Martin 2, Sarah<br />
Spurgeon 3, Gary Robinson 3, Mark Smales 3,<br />
Richard Frost 1, Jeff Williams 1, Tom Falcon 1<br />
1Shepherd Neame, Faversham, United Kingdom, 2Newcastle<br />
University, BBTC, Newcastle Upon Tyne, United Kingdom,<br />
3University of Kent, Canterbury, United Kingdom<br />
DESCRIPTION OF TOPIC:<br />
On-line prediction and off -line simulation of<br />
key variables is required <strong>for</strong> <strong>the</strong> advanced<br />
monitoring, control and optimisation of any<br />
process. Application of control strategies requires<br />
development of appropriate ma<strong>the</strong>matical<br />
models describing <strong>the</strong> process. Optimisation<br />
of beer fermentation problems using<br />
advanced modelling techniques has been<br />
carried out previously with <strong>the</strong> computation<br />
of optimal cooling strategies and fl avor modelling.<br />
The work <strong>for</strong> this project is taking place at<br />
Shepherd Neame Ltd. The brewery is currently<br />
enhancing its fermentation process. This<br />
project will aid in analysis, control and optimisation<br />
of <strong>the</strong> fermentation profi le, achieving<br />
and maintaining targets and standards.<br />
The objective of this project is to assess<br />
current fermentation profi les in relation to<br />
cooling strategies and to evaluate how this<br />
profi le can be optimised. Key variables will<br />
be identifi ed and, with <strong>the</strong> use of modelling<br />
techniques, process monitoring, control and<br />
optimisation will be possible.<br />
Elaine Martin<br />
Currently carrying out an EngD focused on<br />
process optimisation in brewing. This is in<br />
association with Newcastle University, <strong>the</strong><br />
University of Kent and Shepherd Neame Ltd.<br />
Acquired an MSc in Biotech and BSc in Environmental<br />
Plant Biotech from UCC, Ireland.<br />
76<br />
P 097<br />
Comparison of growth kinetics,<br />
major metabolites and sensory<br />
profi les in brewing with non-<br />
saccharomyces yeast<br />
Panagiotis Tataridis 1,2, Dimitris Diamantis 1,<br />
Kaliopi Gialitaki 1, Anastassios Kanellis 1,2,<br />
Despina Kechagia 1, Elias Nerantzis 1<br />
1Technological Educational Institute of A<strong>the</strong>ns, Department of<br />
Enology & Beverage Technology, A<strong>the</strong>ns, Greece,<br />
2Pan-Hellinic Union of Registered Enologists, A<strong>the</strong>ns, Greece<br />
DESCRIPTION OF TOPIC:<br />
In <strong>the</strong> last decades, <strong>the</strong> rapid growth of craft<br />
brewing gave rise to <strong>the</strong> experimental use<br />
of non-Saccharomyces yeasts, which were<br />
previously only reported as spoilage microorganisms.<br />
This study explores <strong>the</strong> eff ects of<br />
non-Saccharomyces strains in comparison<br />
with a well known S. cerevisiae commercial<br />
brewing strain. K. <strong>the</strong>rmotolerans strains exhibit<br />
slower growth, with similar results as<br />
<strong>the</strong> S. cerevisiae. T. delbrueckii strains show<br />
slower growth, lower viability and ethanol<br />
yields but produce noticeably higher concentration<br />
of higher alcohols, esters, terpenes and<br />
phenolic aldehydes, giving a distinct fl oral<br />
and fruity aroma and sensory complexity. At<br />
he same time <strong>the</strong>y have lower production of<br />
volatile acidity, acetaldehyde and acetoin and<br />
higher production of glycerol and succinic<br />
acid, depending on <strong>the</strong> strains. P. kluyveri and<br />
C. stellata strains did not show any signifi cant<br />
growth, however, P. kluyveri <strong>for</strong>med a surface<br />
veil and gave powerful and distinct banana<br />
and bubblegum aroma.<br />
Panagiotis Tataridis<br />
Lecturer at <strong>the</strong> Dept. of Enology & Beverage<br />
Technology-TEI of A<strong>the</strong>ns and President of<br />
<strong>the</strong> Pan-Hellenic Union of Registered Enologists.<br />
Enologist and Brewer from <strong>the</strong> TEI of<br />
A<strong>the</strong>ns, with a D.E.A. in Enology-Ampelology<br />
from <strong>the</strong> University of Burgundy (IUVV) and a<br />
Ph.D. from <strong>the</strong> Institut Nationale Polytechique<br />
of Toulouse (ENSIACET). He is specialized<br />
in wine and brewing science, fermentation<br />
technology, process engineering and sensory<br />
evaluation. He has 10 years of academic and<br />
8 years of Quality Manager/Food Safety consultant<br />
experience with 43 papers/communications
P 098<br />
Waste water handling in breweries,<br />
optimization and cost saving opportunities<br />
Holger Schmidt 1<br />
1Endress+Hauser Messtechnik GmbH+Co.KG, Industry<br />
Marketing, Weil am Rhein, Germany<br />
DESCRIPTION OF TOPIC:<br />
We will discuss as well full waste water treatment<br />
or neutralization. We will show ways to<br />
reduce <strong>the</strong> amount of waste water and save<br />
energy and eff orts in <strong>the</strong> treatment of <strong>the</strong><br />
remains.Inline measurement technology like<br />
conductive, optical, physical and chemical<br />
based sensors help to recognize <strong>the</strong> actual<br />
process status and react immediately if values<br />
are out of <strong>the</strong> specifi cation.How to avoid<br />
product losses and waste load. Focussed<br />
energy any chemical consumption lead to<br />
direct savings in <strong>the</strong> treatment. The correct<br />
measurement and reporting of quality and<br />
quantity of <strong>the</strong> waste water enable pinpointed<br />
activities and negotiations with municipality.<br />
Examples of successfully operating waste<br />
water plants from breweries and o<strong>the</strong>r beverage<br />
plants deliver inside views.Using state of<br />
<strong>the</strong> art automation technologies help to improve<br />
<strong>the</strong> cost situation and to lower <strong>the</strong> carbon<br />
footprint of a brewery. See <strong>the</strong> opportunities<br />
of using actually available technologies<br />
from <strong>the</strong> automation perspective.<br />
Holger Schmidt<br />
Brewer and Malter at Beck&co Bremen, Dipl.<br />
Brewmaster at Weihenstephan Sales and<br />
Project Engineer <strong>for</strong> KHS, APV and Huppmann<br />
with national and international activities.<br />
Since 2003 as Global Industry Manager<br />
Food and Beverage responsible <strong>for</strong> <strong>the</strong> interface<br />
between <strong>the</strong> global Food and Beverage<br />
producers, <strong>the</strong> plant and machine builder and<br />
Endress+Hauser. Active work in EHEDG, ME-<br />
BAK, VDMA and ZVEI commitees.<br />
P 099<br />
<strong>Brewery</strong> carbon footprint:<br />
A case study<br />
Gordon Jackson 2, Anastassia Johnson 1<br />
1Campden BRI, Environmental, Nutfi eld, United Kingdom,<br />
2Campden BRI, Brewing Services, Nutfi eld, United Kingdom<br />
DESCRIPTION OF TOPIC:<br />
Campden BRI will present <strong>the</strong> results of <strong>the</strong><br />
project to calculate <strong>the</strong> carbon footprint of a<br />
brewery in <strong>the</strong> UK. The presentation will discuss<br />
in detail:<br />
• <strong>the</strong> stages of <strong>the</strong> project: defi ning <strong>the</strong><br />
boundary of <strong>the</strong> carbon footprint, greenhouse<br />
gases included and identifying <strong>the</strong> sources of<br />
<strong>the</strong> emissions needed to be incorporated<br />
• <strong>the</strong> methodology used<br />
• main data points and fi ndings<br />
• conclusions from <strong>the</strong> study and actions <strong>for</strong><br />
<strong>the</strong> future<br />
Carbon footprinting is presented as <strong>the</strong> fi rst<br />
step in managing a brewery‘s greenhouse<br />
gas emissions and identifying <strong>the</strong> main areas<br />
<strong>for</strong> effi ciency improvement. The presentation<br />
will highlight how measuring and analysing a<br />
company‘s emissions proves useful in identifying<br />
resource intensive hot spots across<br />
operations and in<strong>for</strong>ms reduction strategies.<br />
The presentation will also include a comparison<br />
of <strong>the</strong> per<strong>for</strong>mance of <strong>the</strong> brewery with<br />
data from o<strong>the</strong>r breweries.<br />
Gordon Jackson<br />
Gordon has worked <strong>for</strong> Campden BRI <strong>for</strong><br />
many years and in a variety of roles. He is<br />
currently Head of Brewing Services which covers<br />
environmental issues <strong>for</strong> breweries. This<br />
work has included collaborating in benchmarking<br />
of energy and water use in breweries<br />
in Europe and elsew<strong>here</strong>. It has also included<br />
assisting breweries to calculate <strong>the</strong>ir carbon<br />
footprint.<br />
P 100<br />
Improved strategies to reduce <strong>the</strong><br />
caustic consumption in breweries<br />
Joris de Grooth 1, Jens Potreck 1,<br />
André Mepschen 1<br />
1Pentair, Research & Development, Enschede, The Ne<strong>the</strong>rlands<br />
DESCRIPTION OF TOPIC:<br />
Brewers often aim to reduce <strong>the</strong> use of<br />
cleaning agents. This reduction will have a<br />
benefi cial eff ect on <strong>the</strong> footprint only when<br />
<strong>the</strong> effi ciency is not compromised. In this<br />
work we will demonstrate <strong>the</strong> necessity of<br />
caustic cleaning steps, correlating empirical<br />
data with a fouling model based on specifi c<br />
molecular interactions. Low level of alkalinity<br />
is needed to remove <strong>the</strong> majority of <strong>the</strong><br />
fouling layer. We explain why higher levels of<br />
alkalinity are benefi ciary <strong>for</strong> <strong>the</strong> removal of<br />
residual fouling during an occasional oxidation<br />
step. These results show that in order not<br />
to lose <strong>the</strong> overall per<strong>for</strong>mance, some levels<br />
of caustic are needed. Lowering <strong>the</strong> overall<br />
consumption will thus rely on <strong>the</strong> reuse of<br />
chemicals. We will demonstrate two viable<br />
strategies. One is stacking of caustic cleaning<br />
solutions <strong>for</strong> beer fi lters. The 2nd one is by<br />
means of a new hollow fi ber NF membrane,<br />
capable of clarifying CIP solutions. The unique<br />
hollow fi ber geometry allows fi ltration without<br />
any pre-treatment<br />
Joris de Grooth<br />
Ir. Joris de Grooth obtained his master degree<br />
in Chemical Engineering at <strong>the</strong> Univeristy<br />
of Twente in The Ne<strong>the</strong>rlands. During his<br />
study he focused on organic and analytical<br />
chemistry, fi nishing with a master <strong>the</strong>sis on<br />
Nanoparticle Based Immunoassays. In 2008,<br />
he started working at <strong>the</strong> R&D department of<br />
Norit X-Flow, fi rst focusing on new cleaning<br />
methods <strong>for</strong> beer fi ltration. Nowadays, he is<br />
mainly involved in <strong>the</strong> development of new<br />
polymeric membranes and processes t<strong>here</strong>of.<br />
77 34 TH EBC CONGRESS · LUXEMBOURG 26 – 30 MAY 2013
P 101<br />
Holistic heat storage system <strong>for</strong><br />
breweries - interface <strong>for</strong> renewable<br />
energy resources<br />
Tobias Becher 1, Klaus Wasmuht 1<br />
1Ziemann International GmbH, Process Technology, R+D,<br />
Ludwigsburg, Germany<br />
DESCRIPTION OF TOPIC:<br />
Breweries are highly dependent on <strong>the</strong> environment<br />
<strong>for</strong> resources and consequently<br />
have a high level of environmental awareness.<br />
T<strong>here</strong><strong>for</strong>e an energy supply system is<br />
introduced, which works using heat storage.<br />
It implies <strong>the</strong> change from steam-driven to<br />
hot-water-driven operation. Utilization of fossil<br />
fuels is reduced and can be replaced by<br />
renewable energy resources. Natural sources<br />
such as solar <strong>the</strong>rmic energy requires a <strong>for</strong>m<br />
of storage. The heat storage system functions<br />
with <strong>the</strong>rmal stratifi cation which is advantageous<br />
<strong>for</strong> energy requiring processes. The<br />
functionality of <strong>the</strong> system is explained by<br />
means of scientifi c survey, scenario simulation<br />
and lead user experience. <strong>Brewery</strong> equipment<br />
and technology can easily be adapted.<br />
With <strong>the</strong> holistic approach, <strong>the</strong> successful<br />
principle of energy recovery is extended<br />
and intelligently linked to all energy sources<br />
and sinks arising in <strong>the</strong> industrial operation.<br />
A functional interface <strong>for</strong> utilization of any<br />
available renewable energy is described.<br />
Tobias Becher<br />
Tobias Becher graduated as diploma engineer<br />
of brewing science and beverage technology<br />
in 2001 at <strong>the</strong> Technical University of Munich<br />
in Freising-Weihenstephan. He made an apprenticeship<br />
as brewer and maltster be<strong>for</strong>e<br />
and worked afterwards as process engineer<br />
<strong>for</strong> beer fi ltration systems and as technical<br />
consultant <strong>for</strong> environmental issues of <strong>the</strong><br />
brewing sector. Since 2005 he is employed by<br />
Ziemann. Today he is deputy director of <strong>the</strong><br />
department Process Technology at Ziemann<br />
International GmbH.<br />
P 102<br />
From waste to renewable energy<br />
source - new technical approach in<br />
wet fermentation of brewers spent<br />
grains<br />
Benjamin Haeff ner 1, Karl Sommer 1<br />
1Technische Universität München, Center of Life Sciences<br />
Weihenstephan, Chair of Process Engineering of Disperse<br />
Systems, Freising, Germany<br />
DESCRIPTION OF TOPIC:<br />
<strong>Brewery</strong> spent grains become with its high<br />
potential <strong>the</strong> key-factor in a cost neutral brewery<br />
waste discharge if you know how to get<br />
access to this stored energy. With <strong>the</strong> wet fermentation<br />
and <strong>the</strong> unit operation milling <strong>the</strong><br />
potential of <strong>the</strong> waste can be used optimally.<br />
T<strong>here</strong><strong>for</strong>e <strong>the</strong> process has to be optimized in<br />
two parts: First part is to fi nd <strong>the</strong> right milling<br />
parameters to save energy while <strong>the</strong> milling.<br />
Second part is to increase <strong>the</strong> biomass<br />
support while <strong>the</strong> fermentation. T<strong>here</strong>by <strong>the</strong><br />
dwell time can be shorted in <strong>the</strong> fi xed bed reactor<br />
despite <strong>the</strong> same degree of degradation<br />
and <strong>the</strong> eff ectiveness rises. With <strong>the</strong> results<br />
from <strong>the</strong> work packages, we can achieve <strong>the</strong><br />
best energy effi ciency of <strong>the</strong> overall process.<br />
Benjamin Haeff ner<br />
Benny was born in June 1979. He made an apprenticeship<br />
to specialist in food technology<br />
at <strong>the</strong> company Döhler. He received a degree<br />
as Diploma Engineer (MSc) in Food and Beverage<br />
Technology from TUM - Weihenstephan<br />
in 2006. He started his career at <strong>the</strong> Chair of<br />
Process Engineering of Disperse Systems as<br />
a technical engineer in wet/dry grinding and<br />
fermentation processes. He is an expert in<br />
comminution of organic materials and gives<br />
lectures in Engineering Science Fundamentals<br />
of apparatus.<br />
78<br />
P 104<br />
Characterisation of co-products as<br />
a potential value added product <strong>for</strong><br />
brewing and distilling<br />
Dawn L Maskell 1, Jane White 1, Julio E Traub 1,<br />
Nik Willoughby 1<br />
1Heriot-Watt University, School of Engineering and Physical<br />
Sciences, Edinburgh, United Kingdom<br />
DESCRIPTION OF TOPIC:<br />
Co-products from <strong>the</strong> brewing and distilling<br />
industries have long been sold on, usually to<br />
farmers <strong>for</strong> use as feed or fertiliser. The increasing<br />
use of biomass to produce fuel may<br />
see <strong>the</strong> market <strong>for</strong> co-products become saturated<br />
in <strong>the</strong> future. It is suggested that t<strong>here</strong><br />
is an opportunity to improve <strong>the</strong> value of coproducts<br />
by extracting components of interest.<br />
The fi rst steps in this project are to characterise<br />
co-products in terms of <strong>the</strong> positive<br />
attributes, including metal ions and protein.<br />
Samples of co-products, including excess<br />
brewers yeast, pot ale and spent wash were<br />
collected from sites around Scotland and examined<br />
<strong>for</strong> <strong>the</strong>ir positive attributes which may<br />
be utilised to produce a high value product<br />
<strong>for</strong> <strong>the</strong> foodstuff or feedstock markets. The<br />
presence of anti-nutritional factors will also<br />
be discussed. The in<strong>for</strong>mation gat<strong>here</strong>d from<br />
<strong>the</strong>se analyses may <strong>the</strong>n be used to determine<br />
<strong>the</strong> suitability of co-products <strong>for</strong> particular<br />
markets.<br />
Dawn L Maskell<br />
Gained a BSc (Hons) in Brewing and Distilling<br />
from Heriot-Watt University and was awarded<br />
<strong>the</strong> J and J Morrison Scholarship to complete<br />
a PhD in Brewing Yeast Physiology at Ox<strong>for</strong>d<br />
Brookes University in association with<br />
Scottish Courage Brewing Limited. She was<br />
a Teaching Fellow at <strong>the</strong> International Centre<br />
<strong>for</strong> Brewing and Distilling, be<strong>for</strong>e taking up a<br />
post as Postdoctoral Research Associate in<br />
<strong>the</strong> School of Engineering and Physical Sciences,<br />
also at Heriot-Watt University.
P 105<br />
Utilization of brewer‘s spent grain<br />
as medium <strong>for</strong> growing Rhodococcus<br />
erythropolis<br />
Enriqueta Martinez-Rojas 1, Renate<br />
Buckhard 2, Leif-Alexander Garbe 2<br />
1VLB Berlin, Berlin, Germany, 2TU-Berlin, Berlin, Germany<br />
DESCRIPTION OF TOPIC:<br />
T<strong>here</strong> is currently huge interest in developing<br />
novel syn<strong>the</strong>tic strategies <strong>for</strong> <strong>the</strong> production<br />
of chemical that can be applied in an environmentally<br />
friendly manner. Brewer‘s spent<br />
grain represents <strong>the</strong> major co-product in<br />
brewing industry and is considered to be good<br />
sources of un-degradable protein, as well as<br />
lignin, carbohydrate and arabinoxylans. Draff<br />
is considered an excellent media <strong>for</strong> growing<br />
mushroom and could be used as culture media<br />
<strong>for</strong> ano<strong>the</strong>r microorganisms like bacteria<br />
<strong>for</strong> example R. erythropolis that posses <strong>the</strong><br />
ability to metabolize diff erent carbon sources<br />
and to be particularly adaptive to utilize xenophobic<br />
compounds. This widely occurring organism<br />
has considerable environmental and<br />
biotechnological importance due to its broad<br />
metabolic diversity and array of unique enzymatic<br />
capabilities. Our results provided a very<br />
attractive opportunity to apply <strong>the</strong> renewable<br />
raw material with an effi cient lab-scale process<br />
to obtain high valuable chemicals.<br />
Enriqueta Martinez-Rojas<br />
1999 Diplom in Biochemistry and Microbiology<br />
at <strong>the</strong> Universidad Autonoma de Nuevo<br />
Leon, Mexico. 2002 Research visitor at <strong>the</strong><br />
Free University of Berlin, Berlin Germany .<br />
2007 Ph.D. at <strong>the</strong> Institute <strong>for</strong> Biotechnology.<br />
TU- Berlin, Berlin Germany. 2008-2011 Scientist<br />
at Celares GmbH, Berlin Germany. 2012<br />
Research visitor at <strong>the</strong> University of Florida<br />
since 2011 Post-Doc at <strong>the</strong> Research and<br />
Teaching Institute <strong>for</strong> Brewing in Berlin, Germany.<br />
P 106<br />
Vital role of brewery in-house R&D<br />
Adam Broz 1, Petr Kosin 1, Jan Savel 1<br />
1Budejovicky Budvar, n.p., Ceske Budejovice, Czech Republic<br />
DESCRIPTION OF TOPIC:<br />
Strong global players, single breweries as<br />
well as craft brewers compete <strong>for</strong> customer‘s<br />
favour. High and standard beer quality is regarded<br />
to be obvious on developed markets.<br />
Everyday routine showed <strong>the</strong> need of complying<br />
check-out quality system including rapid<br />
screening methods. Many of analytical methods<br />
must be adapted <strong>for</strong> use in local conditions<br />
of a particular brewery. New methods<br />
developed in own brewery laboratory could<br />
help to solve problems with diff erent crops of<br />
raw materials. Research results are immediately<br />
used <strong>for</strong> process control. In long-term<br />
view, progressive replacement of time-worn<br />
technology could be a diffi cult task if all qualitative<br />
characteristics, especially <strong>the</strong> taste,<br />
must be kept. Our own brewery research and<br />
development department which is orientated<br />
to current priorities of brewer‘s need plays vital<br />
role in company‘s competitiveness. Experience<br />
is demonstrated on examples of quality<br />
system, methods development and interpretation<br />
of antioxidants knowledge.<br />
Adam Broz<br />
Adam Broz received engineering degree in<br />
brewing and malting from Institute of Chemical<br />
Technology in Prague in 1999. He has been<br />
working <strong>for</strong> Budejovicky Budvar brewery in<br />
Ceske Budejovice, Czech Republic, since his<br />
graduation. He worked as a technician, chief<br />
of brew house, plant technologist and deputy<br />
brew master. Since 2009, he has been working<br />
as a production and technical director.<br />
He graduated doctor studies in biotechnology<br />
from Institute of Chemical Technology in<br />
Prague in 2010.<br />
P 107<br />
The glycemic index – chance or<br />
threat <strong>for</strong> <strong>the</strong> beverage industry?<br />
Moritz Krahl 1<br />
1Radeberger Gruppe KG, Frankfurt am Main, Germany<br />
DESCRIPTION OF TOPIC:<br />
Jenkins introduced <strong>the</strong> concept of <strong>the</strong> glycemic<br />
index in <strong>the</strong> 1970s. In <strong>the</strong> following years<br />
diseases caused by poor or unbalanced diets<br />
have led to severe problems in <strong>the</strong> Western<br />
world. In this paper <strong>the</strong> concept of <strong>the</strong> GI and<br />
<strong>the</strong> fundamentals of <strong>the</strong> carbohydrate metabolism<br />
are explained. Measured glycemic<br />
index values <strong>for</strong> diff erent types of beverages,<br />
both alcoholic and non-alcoholic, are compared<br />
with <strong>the</strong> amounts of sugar present in <strong>the</strong>se<br />
beverages. In recent years consumers and<br />
NGOs are getting aware of <strong>the</strong> possible negative<br />
health impact of high GI beverages. The<br />
industry needs to focus on low calorie and<br />
low GI products as an alternative to traditional<br />
beverages. Sweeteners and low GI carbohydrates<br />
can be used in this regard. Advantages<br />
of diff erent alternatives like steviol glycosides,<br />
polyols, erythritol and isomaltulose are<br />
discussed. By combining diff erent sweeteners<br />
and using <strong>the</strong>ir synergistic eff ects, taste<br />
profi les close to sucrose sweetened beverages<br />
can be guaranteed.<br />
Moritz Krahl<br />
Dr. Moritz Krahl was born in Schwetzingen,<br />
Germany. In 2004 he graduated with a B.Sc.<br />
degree and in 2005 with a Dipl.-Ing. (Graduate<br />
engineer) degree. From 2005 to 2010 Moritz<br />
did his Ph.D. at <strong>the</strong> Institute <strong>for</strong> Brewing and<br />
Beverage Technology. From May 2010 until<br />
September 2011 he worked as head engineer<br />
<strong>for</strong> plant and process optimization <strong>for</strong> MEG.<br />
In October 2011 Dr. Moritz Krahl joined <strong>the</strong><br />
Radeberger Group with <strong>the</strong> key responsibility<br />
in product and process development <strong>for</strong> new<br />
beverages.<br />
79 34 TH EBC CONGRESS · LUXEMBOURG 26 – 30 MAY 2013
P 108<br />
Transfer of Nitrate into Beer during<br />
Dry-hopping<br />
Stefan Hanke1, Annika Lagemann2, Georg Stettner3 1Bitburger Braugruppe GmbH, Versuchsbrauerei, Bitburg,<br />
Germany, 2Bitburger Braugruppe GmbH, Central Laboratory,<br />
Bitburg, Germany, 3Bitburger Braugruppe GmbH, Technology<br />
and Quality, Bitburg, Germany<br />
DESCRIPTION OF TOPIC:<br />
Within <strong>the</strong> last years <strong>the</strong> success of <strong>the</strong> US<br />
Craft beers, which are partial extremely hopped,<br />
also inspired brewers outside <strong>the</strong> US.<br />
The technique of dry-hopping is a very old<br />
method to enhance fl avor properties of beer<br />
and was re-established by <strong>the</strong> craft movement.<br />
Since 2012, this hopping method is also<br />
offi cial allowed to be used in Germany and so<br />
<strong>the</strong> amount of dry-hopped beers in <strong>the</strong> market<br />
increases steadily. During dry-hopping<br />
fl avor and phenolic compounds migrate into<br />
<strong>the</strong> beer. The migration of o<strong>the</strong>r compounds<br />
(like nitrate) was not investigated until today.<br />
The limit value <strong>for</strong> nitrate in drinking water is<br />
set to 50 ppm. In our study beers were produced<br />
with diff erent dry-hopping regimes (up<br />
to 400 g/hl) and nitrate was measured in <strong>the</strong><br />
fi nal beers. It could be shown that signifi cant<br />
amounts of nitrate migrate into <strong>the</strong> beer and<br />
that <strong>the</strong> legal limit can be reached. This shows<br />
that nitrate level in hops should be considered<br />
in case of dry-hopping.<br />
Stefan Hanke<br />
From 1999-2004 he studied brewing science<br />
in Weihenstephan, graduating with an Dipl.-<br />
Ing. degree. 2004-2010 he was scientifi c assistant<br />
at TU Munich/Weihenstephan and during<br />
this time responsible <strong>for</strong> <strong>the</strong> small scale<br />
brewing facilities and GC/HPLC department<br />
at <strong>the</strong> institute. In 2010 he received a PhD in<br />
brewing technology. Since 2010 he is head of<br />
<strong>the</strong> pilot plant of <strong>the</strong> Bitburg Brewing Group,<br />
Bitburg, Germany and t<strong>here</strong><strong>for</strong>e responsible<br />
<strong>for</strong> R&D issues of affi liated breweries.<br />
P 109<br />
Beer and cardiovsculare system –<br />
immediate respons after intake<br />
Ida Leskosek-Cukalovic 1, Sasa Despotovic 1,<br />
Mirjana Platisa 2, Vera Gal 2, Zorica Nestorovic 2,<br />
Ljiljana Gojkovic-Bukarica 3, Mile Veljovic 1,<br />
Sonja Pecic 1, Viktor Nedovic 1<br />
1Institute <strong>for</strong> Food Technology and Biochemistry, Faculty of<br />
Agriculture, University of Belgrade, Belgrade, Serbia, 2Institute<br />
of Biophysics, Faculty of Medicine, University of Belgrade,<br />
Belgrade, Serbia, 3Institute of Clinical Pharmacology, Faculty<br />
of Medicine, University of Belgrade, Belgrade, Serbia<br />
DESCRIPTION OF TOPIC:<br />
Numerous long-term studies have described<br />
<strong>the</strong> impact of alcohol beverages on cardiovascular<br />
diseases. In this study it was investigated<br />
<strong>the</strong> eff ect of acute intake of beer on<br />
blood pressure and heart rate variability. The<br />
HRV analysis and ECG was recorded in supine<br />
position in 18 young healthy subjects. Results<br />
of HRV analysis indicate on mild infl uence of<br />
beer on <strong>the</strong> autonomic cardiac control. The infl<br />
uence of beer on heart rhythm, examined by<br />
<strong>the</strong> HRV analysis which quantify <strong>the</strong> activity of<br />
<strong>the</strong> autonomic nervous system on <strong>the</strong> heart,<br />
was not signifi cant when all <strong>the</strong> subjects were<br />
analyzed toge<strong>the</strong>r. However, <strong>the</strong> diff erences<br />
between subjects were signifi cant <strong>for</strong> all variables.<br />
T<strong>here</strong><strong>for</strong>e, it was analyzed <strong>the</strong> eff ect of<br />
beer <strong>for</strong> each subject separately. In 10 out of<br />
18 subjects, beer drinking signifi cantly changed<br />
HR and/or HRV measures. The eff ect of<br />
beer is <strong>the</strong> increase of HR and corresponding<br />
decrease of autonomic modulation refl ected<br />
on decrease of spectral powers and parasympa<strong>the</strong>tic<br />
activity.<br />
Ida Leskosek-Cukalovic<br />
Received BSc and MSc in Technical sciences<br />
at <strong>the</strong> Faculty of Technology and Metalurgy<br />
and PhD in Biotechnical sciences at <strong>the</strong> Faculty<br />
of Agriculture, Universitu of Belgrade,<br />
Serbia. Since 1978 emploied at <strong>the</strong> Faculty<br />
of Agriculture, Institute <strong>for</strong> Food technology<br />
and Biochemistry, Belgrade. Currently a full<br />
Professor of Brewing technology, Chief of<br />
Brewing Div. and Head of Preservation and<br />
Fermentation Technologies Dep. Memeber of<br />
<strong>the</strong> Menagement board of <strong>the</strong> Serbian Nutrition<br />
society.<br />
80
GUIDE TO BEERS AND BREWERIES<br />
Bitburger<br />
Bitburg <strong>Brewery</strong> was founded in 1817 and is, now in <strong>the</strong> 7th generation,<br />
still family-owned. The brewery has committed itself to <strong>the</strong> highest<br />
quality on all levels driven by a commitment born out of tradition<br />
and conviction. The full-bodied, golden, Bitburger Premium Pils is of<br />
course brewed according to <strong>the</strong> German Purity Law. Its dry-fi nished,<br />
hoppy taste has secured Bitburger Premium Beer <strong>the</strong> position as<br />
Germanys No. 1 draft beer.<br />
Brasserie National mit Bofferding & Battin<br />
The Brasserie Nationale, Bofferding, is <strong>the</strong> result of <strong>the</strong> merger of two<br />
family businesses, <strong>the</strong> oldest anchored in Luxembourg since 1764.<br />
Over <strong>the</strong> centuries and thanks to a long-term strategy based on impeccable<br />
quality beer, <strong>the</strong> Brasserie Nationale has become <strong>the</strong> main<br />
<strong>Brewery</strong> of <strong>the</strong> Grand Duchy of Luxembourg.<br />
• BOFFERDING:<br />
A 100% NATURAL QUALITY BEER.<br />
Brewed from <strong>the</strong> fi nest raw material, Bofferding beer is made<br />
with only pure malt and pure hops, without any additives or preservatives.<br />
The Bofferding <strong>Brewery</strong> proudly produces a 100% natural<br />
beer.<br />
Only four ingredients: Water, Malt, Hops and Yeast.<br />
• BATTIN:<br />
A BEER BREWED LIKE IN THE OLD DAYS<br />
Battin Gambrinus is a home-made beer, brewed like in days gone<br />
by, from an ancestral recipe. The quality of ingredients is primordial<br />
to get this nice and fresh beer, especially appreciated <strong>for</strong><br />
its delicious malt aroma and fi ne white head.<br />
Only four ingredients: Water, Malt, Hops and Yeast.<br />
82<br />
Diekirch Premium<br />
As a company, Diekirch strives to stay close to consumers and <strong>the</strong>ir<br />
needs. It has evolved with <strong>the</strong> times and aims to always meet <strong>the</strong> new<br />
expectations of consumers…<br />
Diekirch Premium is a smooth lager beer made with superior quality<br />
malt and hops and brewed naturally with pure spring water from <strong>the</strong><br />
green hills of Diekirch. Since 1871, this recipe has given <strong>the</strong> beer its<br />
unique character, sweet, natural fl avours and pure white foam head.<br />
Diekirch (4.8% ABV) is <strong>the</strong> perfect beer <strong>for</strong> sharing with friends and<br />
enjoying each moment life offers!<br />
Cheers!<br />
Simon<br />
The Brasserie Simon is situated approximately 60 km to <strong>the</strong> north<br />
of Luxembourg City in <strong>the</strong> town of Wiltz and it was founded in 1824.<br />
The Simon family acquired <strong>the</strong> brewery in 1906. The current owner is<br />
Ms. Betty Fontaine (née Simon). Brasserie Simon occupies 3rd place<br />
in <strong>the</strong> ranking of breweries in <strong>the</strong> Grand-Duchy, after <strong>the</strong> Brasserie<br />
Nationale (Bofferding) and Diekirch. Sales volumes are in <strong>the</strong> region<br />
of 21,000 HL annually. The brewery is perhaps best known <strong>for</strong> its<br />
Simon Pils, which is a traditional clean-drinking pilsener brewed to<br />
a time-proven recipe. In 1999, <strong>the</strong> brewery successfully launched a<br />
spelt-adjunct beer, Simon Dinkel.
THE EUROPEAN MALTING INDUSTRY ASSOCIATION<br />
Rue du Trone 98<br />
1050 Brussels<br />
www.euromalt.be<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
<br />
83 34 TH EBC CONGRESS · LUXEMBOURG 26 – 30 MAY 2013
LIST OF EXHIBITORS AND SPONSORS<br />
COMPANY CITY COUNTRY STAND NO<br />
Aber Instruments Ltd, Aberystwyth UNITED KINGDOM 16<br />
Actemium Veghel THE NETHERLANDS 34<br />
A. Handtmann Armaturenfabrik Biberach GERMANY 41<br />
Anton Paar GmbH Graz AUSTRIA 31<br />
AUSTRIA JUICE Group Allhartsberg AUSTRIA 37<br />
BD Biosciences Erembodegem BELGIUM 14<br />
BIOTECON Diagnostics GmbH Berlin GERMANY Sponsor<br />
BRAUWELT – Fachverlag Hans Carl GmbH Nürnberg GERMANY 46<br />
CENTEC GMBH Maintal GERMANY 49<br />
COMPTOIR AGRICOLE HOPS Hochfelden FRANCE 32<br />
drinktec München GERMANY 58<br />
DSM Food Specialities Delft THE NETHERLANDS 61<br />
DuPont Le Grand Saconnex SWITZERLAND 59<br />
Ellutia Ely (Cambs.) UNITED KINGDOM 25<br />
Endress+Hauser Messtechnik GmbH+Co. KG Weil am Rhein GERMANY Sponsor<br />
GEA <strong>Brewery</strong> Systems GmbH Kitzingen GERMANY Sponsor<br />
Hamilton Bonaduz AG Bonaduz SWITZERLAND 39<br />
IFBM - QUALTECH Vandoeuvre FRANCE 09<br />
KAHO Sint-Lieven RaD Gent BELGIUM 08<br />
Krones AG Neutraubling GERMANY 33<br />
MALTERIE DU CHÂTEAU / CASTLE MALTING Lambermont BELGIUM 02<br />
Meura S.A. Peruwelz BELGIUM 30<br />
Micro Matic S.A. Troisvierges LUXEMBOURG 51<br />
NIZO food research BV Ede THE NETHERLANDS 27<br />
NovaBiotec® Dr. Fechter GmbH Berlin GERMANY 01<br />
Novozymes Bagsvaerd DENMARK Sponsor<br />
Pall GmbH Bad Kreuznach GERMANY 50<br />
PENTAIR PROCESS TECHNOLOGIES Venlo THE NETHERLANDS 44<br />
PureMalt Products Limited East Lothian UNITED KINGDOM 48<br />
RASTAL GmbH & Co. KG Höhr-Grenzhausen GERMANY Sponsor<br />
r-biopharm Darmstadt GERMANY 1<br />
Scanenergi Solutions Kolding DENMARK 36<br />
Siebel Institute of Technology & World Brewing Academy Chicago UNITED STATES OF AMERICA 26<br />
Siemens AG Food & Beverage Nürnberg GERMANY 60<br />
Skalar Analytical Breda THE NETHERLANDS 17<br />
Steinfurth - Rycobel Essen / Deerlijk GERMANY / BELGIUM 29<br />
The Brewers of Europe EBC Brussels BELGIUM 46<br />
The Institute of Brewing & Distilling London UNITED KINGDOM 40<br />
Thermo Fisher Scientifi c Vantaa FINLAND 10<br />
The Scandinavian School of Brewing København V. DENMARK 03<br />
The University of Nottingham Leicestershire UNITED KINGDOM 38<br />
Töpfer Kulmbach GmbH Kulmbach GERMANY 45<br />
VELP SCIENTIFICA Usmate ITALY 12<br />
Verlag W. Sachon GmbH + Co. KG Mindelheim GERMANY Sponsor<br />
Versuchs- und Lehranstalt für Brauerei in Berlin (VLB) e.V. Berlin GERMANY 13<br />
VTT Technical Research Centre of Finland VTT Espoo FINLAND 28<br />
WALLART sarl Wambrechies FRANCE Sponsor<br />
WEYERMANN SPECIALTY MALTS Bamberg GERMANY 47<br />
84
EXHIBITION PLAN<br />
85 34 TH EBC CONGRESS · LUXEMBOURG 26 – 30 MAY 2013
Born to find out<br />
Solutions <strong>for</strong> Laboratory<br />
and Process:<br />
Wort | Extract | Alcohol | CO 2 | O 2<br />
Density | Specific Gravity | Turbitity<br />
pH | Color | Viscosity<br />
Visit us at<br />
Booth no. 31<br />
Anton Paar GmbH<br />
Tel.: 0043 316 257-0<br />
www.anton-paar.com
EXHIBITOR / SPONSOR GUIDE<br />
ABER INSTRUMENTS LTD BOOTH NO: 16<br />
Aber Instruments will exhibit <strong>the</strong>ir range of competitively priced<br />
Compact®Yeast Monitors. Recognized as <strong>the</strong> most accurate and<br />
reliable instruments <strong>for</strong> measuring live yeast cell concentration<br />
on-line, <strong>the</strong> technology results in more consistent fermentation<br />
processes with fast and precise measurement.<br />
5 Science Park<br />
SY23 3AH<br />
Aberystwyth<br />
United Kingdom<br />
Phone: +44 1970636300<br />
sales@aberinstruments.com<br />
www.aberinstruments.com<br />
A. HANDTMANN ARMATURENFABRIK BOOTH NO: 41<br />
From brewhouse to bottling: A leading supplier of valves, fi ttings<br />
and process equipment <strong>for</strong> <strong>the</strong> brewing industry since 1873. Tailor<br />
made product pipework, tank equipment, CIP plants, fi ltration and<br />
stabilization. It is our passion <strong>for</strong> your project, from planning to<br />
completion.<br />
Axel Jany<br />
Arthur-Handtmann-Str. 23<br />
88400<br />
Biberach<br />
Germany<br />
Phone: +49 73513424542<br />
axel.jany@handtmann.de<br />
www.handtmann.de<br />
AUSTRIA JUICE GROUP BOOTH NO: 37<br />
In 2012 Ybbstaler Fruit Austria and AGRANA Juice Holding joined<br />
to become AUSTRIA JUICE t<strong>here</strong><strong>for</strong>e emerging as a leader in <strong>the</strong>ir<br />
fi eld. Austria Juice produces beverage compounds, juice concentrates,<br />
fruit juice fl avors, fruit purees and NFC juices <strong>for</strong> various<br />
applications in <strong>the</strong> beverage industry.<br />
Stephen Wilkinson<br />
Kroellendorf 45<br />
3365<br />
Allhartsberg<br />
Austria<br />
BIOTECON DIAGNOSTICS GMBH<br />
Phone: +43 744823040<br />
stephen.wilkinson@austriajuice.com<br />
www.austriajuice.com<br />
For safer food - BIOTECON Diagnostics: simply builds up trust.<br />
BIOTECON Diagnostics is well known as a qualifi ed partner in <strong>the</strong><br />
fi eld of molecular and microbiology since 1998. We focus on development,<br />
production and marketing of PCR-based rapid detection<br />
technologies.<br />
BIOTECON Diagnostics GmbH<br />
Hermannswerder 17<br />
14473<br />
Berlin<br />
Germany<br />
Phone: +49 3312300200<br />
bcd@bc-diagnostics.com<br />
www.bc-diagnostics.com<br />
ACTEMIUM BOOTH NO: 34<br />
With <strong>the</strong> advantage of <strong>the</strong> experience of its network, Actemium<br />
designs and integrates solutions dedicated to <strong>the</strong> Beer & Beverage<br />
industry. Its teams have a detailed understanding of production<br />
processes and provide expertise to service your local requirements<br />
as well as global production projects.<br />
Appie Boorsma<br />
Postbus 248<br />
5460 AE<br />
Veghel<br />
The Ne<strong>the</strong>rlands<br />
Phone: +31 413349999<br />
aboorsma@actemium.com<br />
www.actemium.com<br />
ANTON PAAR GMBH BOOTH NO: 31<br />
Anton Paar GmbH was established in 1922 as a one-man<br />
locksmith’s workshop. Today, over 1,300 employees worldwide develop,<br />
produce and distribute high-quality measuring instruments<br />
<strong>for</strong> <strong>the</strong> determination of properties such as density, temperature<br />
and viscosity.<br />
Eveline Schuster<br />
Anton-Paar-Straße 20<br />
8054<br />
Graz<br />
Austria<br />
Phone: +43 3162570<br />
eveline.schuster@anton-paar.com<br />
www.anton-paar.com<br />
BD BIOSCIENCES BOOTH NO: 14<br />
BD Biosciences, a segment of Becton, Dickinson and Company,<br />
is one of <strong>the</strong> world’s leading businesses focused on bringing<br />
innovative tools to life science researchers and clinicians.<br />
Kristien Rasschaert<br />
Erembodegem-Dorp 86<br />
9320<br />
Erembodegem<br />
Belgium<br />
Phone: +32 24009895<br />
kristien_Rasschaert@europe.bd.com<br />
www.bdbiosciences.com/eu<br />
87 34 TH EBC CONGRESS · LUXEMBOURG 26 – 30 MAY 2013
BRAUWELT FACHVERLAG HANS CARL GMBH BOOTH NO: 46<br />
Fachverlag Hans Carl publishes and offers a unique range of specialist<br />
journals and books <strong>for</strong> <strong>the</strong> brewing and beverage sector.<br />
Scientifi c and technical publications by <strong>the</strong> <strong>European</strong> <strong>Brewery</strong> Convention<br />
complete <strong>the</strong> product line-up.<br />
Analytica- EBC goes online in May 2013 (www.analytica-ebc.com)<br />
Our fl agships: BRAUWELT provides in<strong>for</strong>mation <strong>for</strong> <strong>the</strong> global brewing<br />
community, it is published in fi ve languages and read in more<br />
than 160 countries. www.brauweltinternational.com<br />
BrewingScience: All what brewing scientists need to know.<br />
www.brewingscience.de<br />
Just <strong>click</strong> your way to our bookshop CarlLibri at www.carllibri.com<br />
Andernacherstr. 33 a<br />
90411<br />
Nuremberg<br />
Germany<br />
COMPTOIR AGRICOLE HOPS BOOTH NO: 32<br />
Comptoir Agricole offers fi ne Aroma hops of high quality from<br />
Alsace-France. This place and climate conditions are best suited<br />
<strong>for</strong> aroma hop varieties: strisselspalt, <strong>the</strong> most refi ned and <strong>the</strong> new<br />
hops from our breeind program Aramis and Triskel.<br />
Francis Heitz<br />
35, route de Strasbourg<br />
67270<br />
Hochfelden<br />
France<br />
www.hanscarl.com,<br />
www.brauweltinternational.com<br />
Phone: +33 388890909<br />
francis.heitz@cophoudal.fr<br />
www.comptoir-agricole.fr<br />
DSM FOOD SPECIALTIES BOOTH NO: 61<br />
DSM will be showcasing its total brewing solutions portfolio at EBC<br />
2013. These products help brewers fi nd new ways to maximize production<br />
capacity and cost effi ciency whilst meeting growing demand<br />
<strong>for</strong> differentiation. Brewers Clarex: <strong>for</strong> savings that clearly matter.<br />
Brewers Compass: enables effi cient brewing. Filtrase: shorter<br />
fi ltration times.<br />
Alexander Fleminglaan 1<br />
2613 AX<br />
Delft<br />
The Ne<strong>the</strong>rlands<br />
Phone: +31 152793474<br />
info.food@dsm.com<br />
www.dsm.com<br />
88<br />
CENTEC GMBH BOOTH NO: 49<br />
Centec is a supplier <strong>for</strong> inline process sensors (Plato, Brix, Alcohol,<br />
CO 2, O 2) and complete process systems, e.g. <strong>for</strong> Dealcoholization,<br />
HG-Blending, Carbonation, Flash-Pasteurization, CIP, Wort-Aeration,<br />
Yeast Pitching, Softdrink-Multi-Mixer, Water Deaeration and<br />
complete Water treatment systems.<br />
Wilhelm-Röntgen-Straße 10<br />
63477<br />
Maintal<br />
Germany<br />
DRINKTEC (MESSE MÜNCHEN GMBH) BOOTH NO: 58<br />
drinktec is <strong>the</strong> „World’s Leading Trade Fair <strong>for</strong> <strong>the</strong> Beverage and<br />
Liquid Food Industry“. Manufacturers and suppliers from all over<br />
<strong>the</strong> world – global companies and SMEs alike – meet up <strong>here</strong> with<br />
all sizes of producers and retailers of beverages and liquid food<br />
products. Within <strong>the</strong> sector drinktec is regarded as <strong>the</strong> number one<br />
plat<strong>for</strong>m <strong>for</strong> launching new products on <strong>the</strong> world market. At this<br />
event manufacturers present <strong>the</strong> latest technology <strong>for</strong> processing,<br />
fi lling, packaging and marketing all kinds of beverages and liquid<br />
food – raw materials and logistics solutions included. The <strong>the</strong>mes of<br />
beverages marketing and packaging design round off <strong>the</strong> portfolio.<br />
drinktec 2013, which takes place at <strong>the</strong> Messe München exhibition<br />
center in Munich, from September 16 to 20, 2013, is expected to<br />
attract around 1,500 exhibitors from over 70 countries and approximately<br />
60,000 visitors from more than 170 countries.<br />
Messegelände<br />
81823<br />
Munich<br />
Germany www.drinktec.com<br />
DUPONT BOOTH NO: 59<br />
DuPont has been bringing world-class science and engineering to<br />
<strong>the</strong> global marketplace in <strong>the</strong> <strong>for</strong>m of innovative products, materials,<br />
and services since 1802. Through close collaboration with<br />
customers, DuPont combines knowledge and experience with a<br />
passion <strong>for</strong> innovation to deliver unparalleled customer value to <strong>the</strong><br />
marketplace. Within enzyme technology, DuPont offers a range of<br />
ingredients to aid brewing companies achieve <strong>the</strong>ir goals of delivering<br />
superior per<strong>for</strong>mance solutions with greater cost effi ciency and<br />
lower environmental impact.<br />
Isabelle Ardevol<br />
2 Chemin du Pavillon<br />
1218<br />
Le Grand Saconnex<br />
Switzerland<br />
Phone: +49 618118780<br />
info@centec.de<br />
www.centec.de<br />
Phone: +41 227176431<br />
iardevol@solae.com<br />
www.solae.com
ELLUTIA BOOTH NO: 25<br />
Ellutia is a UK based Gas Chromatography Manufacturer, that<br />
produce a range of Gas Chromatographs (GC’s) and specialist<br />
detectors <strong>for</strong> use with GC. Solutions offered include systems <strong>for</strong><br />
<strong>the</strong> analysis of NDMA in malt and beverages, and systems <strong>for</strong> <strong>the</strong><br />
analysis of fl avours and off fl avours eg diacetyl.<br />
xxx<br />
12-16 Sedgeway Business Park,<br />
Witch<strong>for</strong>d<br />
CB6 2HY<br />
Ely (Cambs.)<br />
United Kingdom<br />
Phone: +44 1353669916<br />
info@ellutia.com<br />
www.ellutia.com<br />
GEA BREWERY SYSTEMS GMBH SPONSOR<br />
GEA <strong>Brewery</strong> Systems offers processing equipment and complete<br />
plants to <strong>the</strong> brewing industry. The range of services includes everything<br />
from engineering, delivery and installation up to service and<br />
maintenance as well as realization of greenfi eld projects.<br />
Anja Oltmanns<br />
Heinrich-Huppmann-Str. 1<br />
97318<br />
Kitzingen<br />
Germany<br />
Phone: +49 93213030<br />
gea-brewerysystems@gea.com<br />
www.gea-brewery.com<br />
IFBM-QUALTECH BOOTH NO: 09<br />
ifbm qualtech group (agrobio and a.bio.c) is one of <strong>the</strong> leading technical<br />
research and training centres in europe. We carry out effi cient<br />
consulting, research programmes and analytical services <strong>for</strong> <strong>the</strong><br />
food industry companies. The group offers a wide range of analysis<br />
at an international scale.<br />
Chabane Yahia<br />
7 rue du Bois de la Champelle<br />
BP 86<br />
54503 Vandoeuvre Cedex<br />
France<br />
Phone: +33 383448800<br />
yahia.chabane@ifbm-qualtech.com<br />
www.ifbm.fr<br />
ENDRESS+HAUSER MESSTECHNIK GMBH+CO. KG SPONSOR<br />
The trusted partner of <strong>the</strong> Brewing Industry supplies <strong>the</strong> complete<br />
basket of hygienic process sensors like fl ow, level, pressure, temperature<br />
and several analytical parameters. Inline quality control<br />
and energy monitoring systems are relying on our state of <strong>the</strong><br />
art technology.<br />
Holger Schmidt<br />
Colmarer Strasse 6<br />
79576<br />
Weil am Rhein<br />
Germany<br />
Phone: +49 762197501<br />
holger.schmidt@de.endress.com<br />
www.endress.com<br />
HAMILTON BONADUZ AG BOOTH NO: 39<br />
Hamilton is a manufacturer of products designed <strong>for</strong> <strong>the</strong> measurement<br />
and handling of liquids. Microliter® syringes are outstanding<br />
in all areas of precision liquid handling. In <strong>the</strong> fi eld of analysis<br />
Hamilton is renowned <strong>for</strong> its innovative laboratory and process<br />
electrodes such as pH, Redox, oxygen and conductivity sensors<br />
plus a superior selection of armatures, pH buffer and conductivity<br />
standards. Hamilton supplies HPLC columns and polymer- and<br />
silica-based bulk material. Completing <strong>the</strong> range are semiautomatic<br />
diluter/dispensers and SoftGrip pipettes. Hamilton<br />
also manufactures innovative robotic pipetting systems and OEM<br />
products <strong>for</strong> laboratories in <strong>the</strong> fi elds of biotechnology, analytical<br />
and pharmaceutical chemistry.<br />
xxx<br />
Via Crusch 8<br />
7402<br />
Bonaduz<br />
Switzerland<br />
Phone: +41 816606060<br />
contact@hamilton.ch<br />
www.hamiltoncompany.com<br />
KAHO SINT-LIEVEN RAD BOOTH NO: 18<br />
Characterization of raw materials (malt and hop) and process parameters<br />
in relation to beer fl avour/fl avour instability, implementation<br />
of reliable model systems to determine <strong>the</strong> degree of fl avour deterioration,<br />
development and implementation of novel anti-oxidative<br />
beer production systems.<br />
Guido Aerts<br />
Gebroeders Desmetstraat 1<br />
9000<br />
Gent<br />
Belgium<br />
Phone: +32 92658613<br />
guido.aerts@kahosl.be<br />
www.kahosl.be<br />
89 34 TH EBC CONGRESS · LUXEMBOURG 26 – 30 MAY 2013
KRONES AG BOOTH NO: 33<br />
The Krones Group, headquartered in Neutraubling, Germany, plans,<br />
develops and manufactures machines and complete lines <strong>for</strong> <strong>the</strong><br />
fi elds of process, fi lling and packaging technology and intralogistics.<br />
The company‘s product portfolio is rounded off by corporate<br />
capabilities in in<strong>for</strong>mation technology, factory planning, and<br />
in-house valve manufacture.<br />
Michaela Walter<br />
Böhmerwaldstr. 5<br />
93073<br />
Neutraubling<br />
Germany<br />
Phone: +49 9401703915<br />
michaela.walter@krones.com<br />
www.krones.com<br />
MEURA S.A. BOOTH NO: 30<br />
Meura welcomes you at booth #30 to present its latest innovations:<br />
The Meura2001 Hybrid, <strong>the</strong> Meurabrew and since end 2012, <strong>the</strong><br />
Meurastream. This unique concept enables to reduce <strong>the</strong> <strong>the</strong>rmal<br />
energy by 35% and excess of hot water by 30%, compared to brewhouses<br />
equipped with a vapor condensation!<br />
Rond-Point J.-B. Meura, 1<br />
7600<br />
Péruwelz<br />
Belgium<br />
NOVOZYMES SPONSOR<br />
Novozymes is <strong>the</strong> world leader in bioinnovation. Toge<strong>the</strong>r with<br />
customers across a broad array of industries we create tomorrow’s<br />
industrial biosolutions, improving our customers‘ business and <strong>the</strong><br />
use of our planet‘s resources. Read more at www.novozymes.com.<br />
Krogshoejvej 36<br />
2880<br />
Bagsvaerd<br />
Denmark<br />
Phone: +32 69886988<br />
sales@meura.com<br />
www.meura.com<br />
NIZO FOOD RESEARCH BV BOOTH NO: 27<br />
NIZO food research is an independent contract research company.<br />
We help industry to make better foods and more profi t by developing<br />
technologies <strong>for</strong> innovations in fermentation, fl avour-texture interactions<br />
and food safety. Full confi dentiality and well scoped projects<br />
are essential in our approach.<br />
Kernhemseweg 2<br />
6718 ZB<br />
Ede<br />
The Ne<strong>the</strong>rlands<br />
Phone: +31 3118659511<br />
info@nizo.com<br />
www.nizo.com<br />
Phone: +45 44460000<br />
foodandbeverages@novozymes.com<br />
www.novozymes.com<br />
90<br />
MALTERIE DU CHÂTEAU / CASTLE MALTING BOOTH NO: 02<br />
Castle Malting ®, <strong>the</strong> oldest malting company in Belgium, offers<br />
more than 60 types of base and specialty malts (both conventional<br />
and organic) <strong>for</strong> any type of beer. Today, <strong>the</strong> premium quality of<br />
Château malts’ is <strong>the</strong> guarantee of success <strong>for</strong> more than 1600<br />
brewers in 112 countries worldwide.<br />
Chemin du Couloury 1<br />
4800<br />
Lambermont<br />
Belgium<br />
Phone: +32 87662095<br />
marketing@castlemalting.com<br />
www.castlemalting.com<br />
MICRO MATIC S.A. BOOTH NO: 51<br />
For Micro Matic hygiene and safety are <strong>the</strong> two most important<br />
parameters <strong>for</strong> new developments on draught beer products.<br />
Flexi-draft fi nally guarantees draught beer in top quality, simply<br />
by dispensing through a one-way beer-line and at <strong>the</strong> same time<br />
allowing <strong>the</strong> brewer to use his existing keg fl eet.<br />
Nathalie Proess<br />
18, rue de Drinklange<br />
BP 33<br />
9911<br />
Troisvierges<br />
Luxembourg<br />
NOVABIOTEC® DR. FECHTER GMBH BOOTH NO: 01<br />
NovaBiotec was founded in 1997 and is based in Berlin, Germany.<br />
As an environmental laboratory and consulting offi ce, we offer<br />
different analytical services. For <strong>the</strong> brewing and malting industry,<br />
NovaBiotec developed a testkit <strong>for</strong> <strong>the</strong> determination of beta-glucan<br />
in mash, malt and beers.<br />
Goerzallee 305a<br />
14167<br />
Berlin<br />
Germany<br />
Phone: +35 2979030<br />
nathalie.proess@micro-matic.lu<br />
www.micro-matic.com<br />
Phone: +49 3084718410<br />
glucantest@novabiotec.de<br />
www.novabiotec.de<br />
PALL GMBH BOOTH NO: 50<br />
Pall Corporation is a global fi ltration, separation and purifi cation<br />
leader providing solutions to meet <strong>the</strong> critical fl uid management<br />
needs of customers across <strong>the</strong> broad spectrum of life sciences and<br />
industry.<br />
Planiger Straße 137<br />
55543<br />
Bad Kreuznach<br />
Germany<br />
Phone: +33 327865346<br />
Gregory_Hidot@europe.pall.com<br />
www.pall.com/foodandbev
PENTAIR PROCESS TECHNOLOGIES BOOTH NO: 44<br />
Pentair’s wide range of sustainable solutions includes hygienic and<br />
aseptic valves and components, membrane technology <strong>for</strong> water<br />
and beer purifi cation and fi ltration, continuous beverage processing,<br />
CO 2 recovery, purifi cation and storage, biogas upgrading and<br />
quality control equipment.<br />
Marinus Dammeweg 30<br />
5928 PW<br />
Venlo<br />
The Ne<strong>the</strong>rlands<br />
Phone: +31 7773232300<br />
marketing@haffmans.nl<br />
www.haffmans.nl<br />
RASTAL GMBH & CO. KG SPONSOR<br />
RASTAL is a family-owned German company, which was established<br />
in 1919, excels as a decoration specialist providing brands with<br />
a unique appearance in one of <strong>the</strong> world’s most state-of-<strong>the</strong>-art<br />
decoration centres located in <strong>the</strong> company’s main plant in Höhr-<br />
Grenzhausen.<br />
Rastal-Straße 1<br />
56203<br />
Höhr-Grenzhausen<br />
Germany<br />
Phone: +49 2624160<br />
info@rastal.com<br />
www.rastal.com<br />
SCANENERGI SOLUTIONS BOOTH NO: 36<br />
Scanenergi Solutions is a leading provider of energy saving consultancy<br />
and solutions. Based on <strong>the</strong> ESCO concept we have already<br />
helped more than a 100 breweries to substantially and sustainably<br />
become more competitive by reducing <strong>the</strong>ir energy consumption.<br />
Typically we achieve 10-20% reduction on energy consumption with<br />
IRR > 25%.<br />
Birkemose Allé 33<br />
6000<br />
Kolding<br />
Denmark<br />
Phone: +45 99929200<br />
info@scanergi.com<br />
www.scanergi.com<br />
PUREMALT PRODUCTS LIMITED BOOTH NO: 48<br />
PureMalt’s product range includes refi ned roasted malt beers,<br />
crystal malt beers, and clear and bright malt extracts <strong>for</strong> <strong>the</strong> preparation<br />
of low alcohol beers, alcohol free beers, and malt beverages.<br />
New products increase <strong>the</strong> drinkability of reduced alcohol beers,<br />
and also allow Brewers to create unique black lagers and red beers.<br />
Victoria Bridge<br />
EH41 4BD<br />
Haddington<br />
United Kingdom<br />
Phone: +44 1620824696<br />
info@puremalt.com<br />
www.puremalt.com<br />
R-BIOPHARM BOOTH NO: 01<br />
R-Biopharm AG is an international company with over 500 employees<br />
and subsidiaries in USA, UK, France, Italy, Spain, Denmark,<br />
China, Brazil, Argentina and Australia. The company developed<br />
pioneering solutions in <strong>the</strong> fi elds of clinical diagnostics and food<br />
& feed analysis. R-Biopharm AG is represented worldwide in more<br />
than 100 countries.<br />
An der neuen Bergstraße 17<br />
64297<br />
Darmstadt<br />
Germany<br />
Phone: +49 615181020<br />
info@r-biopharm.de<br />
www.r-biopharm.com<br />
SIEBEL INSTITUTE OF TECHNOLOGY BOOTH NO: 26<br />
Keith Lemcke<br />
1777 North Clybourn Avenue<br />
60614<br />
Chicago<br />
United States of America<br />
Phone: +1 3122550705<br />
klemcke@siebelinstitute.com<br />
www.siebelinstitute.com<br />
91 34 TH EBC CONGRESS · LUXEMBOURG 26 – 30 MAY 2013
SIEMENS AG FOOD & BEVERAGE BOOTH NO: 60<br />
Siemens Industry Sector is <strong>the</strong> world’s leading supplier of innovative<br />
& environmental products & solutions. With automation<br />
technology, industrial software, vertical-market expertise and<br />
services, Siemens enhances its customers’ productivity, effi ciency,<br />
and fl exibility.<br />
Dirk Grafe<br />
Gleiwitzer Straße 555<br />
90475<br />
Nürnberg<br />
Germany<br />
Phone: +49 9118956587<br />
dirk.grafe@siemens.com<br />
www.siemens.com/foodbeverage<br />
STEINFURTH - RYCOBEL BOOTH NO: 29<br />
Steinfurth is established as a market leader <strong>for</strong> electromechanical<br />
quality measuring systems in <strong>the</strong> beverage industry. Rycobel is<br />
an exclusive distributor of this measuring equipment <strong>for</strong> several<br />
<strong>European</strong> countries.<br />
Nijverheidslaan 47<br />
8540<br />
Deerlijk<br />
Belgium<br />
Phone: +32 56782170<br />
info@rycobel.be<br />
www.rycobel.com<br />
THE INSTITUTE OF BREWING & DISTILLING BOOTH NO: 40<br />
A members organisation & educational charity that provides<br />
globally recognised professional qualifi cations/certifi cation. It also<br />
publishes “The Journal of <strong>the</strong> Institute of Brewing” and „The Brewer<br />
& Distiller International”.<br />
Emma Negus-King<br />
33 Clarges Street<br />
W1J 7EE<br />
London<br />
United Kingdom<br />
Phone: +44 2074998144<br />
emma.negus-king@ibd.org.uk<br />
www.ibd.org.uk<br />
THE SCANDINAVIAN SCHOOL OF BREWING BOOTH NO: 03<br />
Since 1925 we have educated 699 Diploma Master Brewers from<br />
32 nations. We have 40 external lecturers, who combine a <strong>the</strong>oretical<br />
knowledge of brewing science with <strong>the</strong> practical experience<br />
of a Master Brewer. In addition we have extensive experience with<br />
tailored training and consultancy.<br />
Gl. Carlsbergvej 16<br />
1799<br />
København V.<br />
Denmark<br />
Phone: +45 33272400<br />
ssb@brewingschool.dk<br />
www.brewingschool.dk<br />
92<br />
SKALAR ANALYTICAL BOOTH NO: 17<br />
Automatic analyzers <strong>for</strong> malt and beer analysis <strong>for</strong> quality<br />
and process samples. Providing accurate and precise results,<br />
methods according EBC and ASBC. Increased sample throughput,<br />
fast results, low running costs. Analyzer runs simultaneously up to<br />
16 applications. Flexible modular design.<br />
Kees Hollaar<br />
Tinstraat 12<br />
4823 AA<br />
Breda<br />
The Ne<strong>the</strong>rlands<br />
Phone: +31 765846486<br />
info@skalar.com<br />
www.skalar.com<br />
THE BREWERS OF EUROPE / EBC BOOTH NO: 46<br />
Founded in 1958 and based in Brussels, The Brewers of Europe is<br />
<strong>the</strong> voice of <strong>the</strong> <strong>European</strong> brewing sector to <strong>the</strong> <strong>European</strong> institutions<br />
and international organisations. Current members are <strong>the</strong> national<br />
brewers’ associations from EU Member States, plus Norway,<br />
Switzerland and Turkey. The mission of The Brewers of Europe is,<br />
‘To equip Europe’s brewers with <strong>the</strong> tools to freely, cost-effectively<br />
and responsibly brew and market beer.’<br />
Rue Caroly 23-25<br />
1050<br />
Brussels<br />
Belgium<br />
THE UNIVERSITY OF NOTTINGHAM BOOTH NO: 38<br />
The University of Nottingham provides world class teaching and<br />
research <strong>for</strong> <strong>the</strong> Brewing and allied industries. Our Postgraduate<br />
Brewing qualifi cations and individual short courses are delivered<br />
through <strong>the</strong> latest innovations in e-learning, and intensive residential<br />
courses. Research strengths: yeast & fermentation technology,<br />
biofuels, malting science and beer fl avour technology.<br />
Linda Vickerstaff<br />
Sutton Bonington Campus<br />
LE12 5RD<br />
Leicestershire<br />
United Kingdom<br />
Phone: +32 25511810<br />
info@brewersofeurope.org<br />
www.brewersofeurope.org<br />
THERMO FISHER SCIENTIFIC BOOTH NO: 10<br />
Gallery Plus Beermaster - a fully automated random<br />
access discrete photometric analyzer with a broad<br />
system reagent menu<br />
Ratastie 2<br />
01620<br />
Vantaa<br />
Finland<br />
Phone: +35 89329100<br />
info.cdx.fi @<strong>the</strong>rmofi sher.com<br />
www.<strong>the</strong>rmoscientifi c.com/beermaster<br />
Phone: +44 1159516245<br />
linda.vickerstaff@nottingham.ac.uk<br />
www.nottingham.ac.uk/<br />
brewingscience
TÖPFER KULMBACH GMBH BOOTH NO: 45<br />
Join us <strong>for</strong> a chat and learn something about our possibilities <strong>for</strong> labels<br />
made from paper, metallised paper, fi lm, PSL, Shrink Sleeves.<br />
Michael Roehle<br />
Am Kreuzstein 5<br />
95326<br />
Kulmbach<br />
Germany<br />
Phone: +49 92217080<br />
michael.roehle@toepfer.de<br />
www.toepfer.de<br />
VERLAG W. SACHON GMBH + CO. KG SPONSOR<br />
BBII adresses special questions of <strong>the</strong><br />
branch being of interest beyond <strong>the</strong> border. With renowned<br />
trade publications in <strong>the</strong> background, <strong>the</strong> Verlag Sachon<br />
can rely on a solid base of top in<strong>for</strong>mation. Well-known<br />
authors of <strong>the</strong> branch cover current topics of brewing and<br />
beverage industry.<br />
Schloß Mindelburg<br />
87714<br />
Mindelheim<br />
Germany<br />
VTT TECHNICAL RESEARCH CENTRE OF FINLAND BOOTH NO: 28<br />
VTT is a globally networked multitechnological research centre. We<br />
can help you improve <strong>the</strong> per<strong>for</strong>mance of your yeast strain, develop<br />
novel beverages, identify microbial contaminants, manage microbes,<br />
create new applications <strong>for</strong> side-streams, or develop environmentally<br />
friendly packaging.<br />
Annika Wilhelmson<br />
P.O. Box 1000<br />
02044<br />
VTT Espoo<br />
Finland<br />
Phone: +358 207227113<br />
annika.wilhelmson@vtt.fi<br />
www.vtt.fi<br />
WEYERMANN® SPECIALTY MALTS BOOTH NO: 47<br />
Meet <strong>the</strong> Weyermann ® malsters <strong>for</strong> a chat! Experience <strong>the</strong> family<br />
tradition and competence and in<strong>for</strong>m yourselves about <strong>the</strong> widest<br />
range of malt on earth!<br />
Silke Thomas<br />
Brennerstrasse 17-19<br />
96052<br />
Bamberg<br />
Germany<br />
Phone: +49 82619990<br />
info@sachon.de<br />
www.sachon.de<br />
Phone: +49 951932200<br />
silke.thomas@weyermann.de<br />
www.weyermannmalt.com<br />
VELP SCIENTIFICA BOOTH NO: 12<br />
VELP Scientifi ca, an Italian company with 30-year experience in<br />
design, manufacture and distribution of high quality lab equipment,<br />
is a key reference worldwide <strong>for</strong> research and quality control labs<br />
offering reliable solutions <strong>for</strong> different industries including food,<br />
feed and beverage testing.<br />
Via Stazione, 16<br />
20865<br />
Usmate<br />
Italy<br />
VERSUCHS- UND LEHRANSTALT FÜR BRAUEREI BOOTH NO: 13<br />
The VLB Berlin is a German institute with a focus on beer brewing.<br />
It was founded in 1883. Today around 130 staff are engaged in <strong>the</strong><br />
fi elds of research, teaching, service and in<strong>for</strong>mation <strong>for</strong> <strong>the</strong> brewing,<br />
malting, beverage and <strong>the</strong>ir supply industries.<br />
Olaf Hendel<br />
Seestraße 13<br />
13353<br />
Berlin<br />
Germany<br />
Phone: +49 30450800<br />
brewmaster@vlb-berlin.org<br />
www.vlb-berlin.org<br />
WALLART SARL SPONSOR<br />
Used equipment <strong>for</strong> <strong>the</strong> beverage industries: Brewhouses, Fermentation<br />
vessels, Filtration lines, Bottling, canning and kegging<br />
equipment.<br />
Parc Activité du Chat<br />
225 Marie Curie<br />
59118<br />
Wambrechies<br />
France<br />
Phone: +39 039628811<br />
inse@velp.it<br />
www.velp.com<br />
Phone: +33 32093671<br />
info@wallart.fr<br />
www.wallart.fr<br />
93 34 TH EBC CONGRESS · LUXEMBOURG 26 – 30 MAY 2013
WE THANK OUR SPONSORS<br />
EVENING PROGRAMME<br />
MONDAY, 27 MAY 2013, starting 18:30 h<br />
Pub Night<br />
GlobalMalt GmbH & Co. KG offers a pub night in Luxembourg’s<br />
bars to registered participants. Please note that <strong>the</strong> number of<br />
tickets is limited. Tickets can be ordered on a fi rst-come, fi rstserved<br />
basis when registering.<br />
TUESDAY, 28 MAY 2013, 16:30 – 20:00 h<br />
Micro-Matic Congress Sundowner<br />
Micro-Matic invites all registered participants to join <strong>the</strong><br />
Congress Sundowner in <strong>the</strong> exhibition and poster areas in <strong>the</strong><br />
Nouveau Centre de Conférences Kirchberg. Enjoy a great evening<br />
with delicious food and some glasses of beer.<br />
THURSDAY, 30 MAY 2013, starting 5 pm<br />
Luxembourg Beer Festival<br />
an unique opportunity to discover <strong>the</strong> great variety of Luxembourg<br />
famous beers served with Luxembourg specialities that will take<br />
place on Place Guillaume starting at 5 pm .<br />
CONGRESS APP<br />
Install <strong>34th</strong> EBC Congress event guide on your smart phone and<br />
fi nd all in<strong>for</strong>mation you need about <strong>the</strong> event:<br />
– Overview of <strong>the</strong> program and posters<br />
– In<strong>for</strong>mation on all presenters<br />
– In<strong>for</strong>mation on tours and evening programme<br />
– Overview of <strong>the</strong> exhibition and beer dispense area including<br />
in<strong>for</strong>mation on <strong>the</strong> participating companies<br />
– Future EBC events<br />
Look up <strong>the</strong> app in your app store (iPhone, Windows, Andoid) – <strong>the</strong><br />
key word is EBC 2013<br />
95 34 TH EBC CONGRESS · LUXEMBOURG 26 – 30 MAY 2013
Organiser<br />
INTERPLAN<br />
Congress, Meeting & Event Management AG<br />
Offi ce Hamburg<br />
Kaiser-Wilhelm-Straße 93<br />
20355 Hamburg, Germany<br />
Tel: +49 - 40 - 3250 9230<br />
Fax: +49 - 40 - 3250 9244<br />
Email: ebc@interplan.de<br />
Scientifi c & Technical Secretariat<br />
EBC<br />
The Brewers of Europe<br />
Rue Caroly 23-25<br />
1050 Brussels,<br />
Belgium<br />
Tel: +32 - 2 - 5511 810<br />
Fax: +32 - 2 - 6609 402<br />
Email: jmb@brewersofeurope.org<br />
Visit <strong>the</strong> EBC 2013<br />
congress webpage