Chromatotron

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Pump Scale example Scale set at 325: Fluid Connections for Pump Model RHSYOOSTYLF Screw tube end fittings into the pump gently by hand only! Raise the solvent reservoir 20 cm if high ambient temperatures or volatile solvents reduce flow rates by forming vapor locks (bubbles in the pump). 7
Pumps are sensitive to abuse: * The cotton filter should be in place at all times when pumping sample solutions or solvent. Solid impurities must not enter the pump. Change the cotton at intervals. Synthetic material sold as "cosmetic puffs" is most suitable. Do not use glass wool; abrasive glass particles will be released into the pump. * Do not leave solutions of compounds in the pump. The piston may bind to the cylinder as the solvent evaporates. * Do not pump hot or supersaturated solutions. Solids may crystallize in the pump. * Do not pump mineral acids. Wash out chloroform (which generates acid on exposure to air and light). * Do not pump toxic materials without suitable precautions to allow for accidental ejection under pressure. NITROGEN FLOW A flow of inert gas is essential. Most compounds will partially oxidize when exposed to sorbents and air. The flowmeter is calibrated for air and is reasonably accurate for nitrogen and argon. If a bubbler is used, place it on one side for high flow rates to allow the gas to pass over rather than through the liquid. Choose a liquid of low volatility, e.g. a phthalate ester. Do not use silicone oil. Silicones may irreversibly contaminate the rotors, preventing adhesion of the sorbent layer. Volatile liquids such as xylene are unsuitable if UV absorption is used for detection of compounds on the rotor. Do not use sulfuric acid in wash bottles. Before using the <strong>Chromatotron</strong>, flush out air with Flowmeter Scale a nitrogen flow of 250-1000 ml/min then reduce to about 15 ml/min (read at center of ball) and maintain this rate during the chromatography. The latter flow rate corresponds to about 1 - 2 bubbles/sec from a 6 mm i.d. tube in a bubbler. Excessively high rates will dislodge sorbent. Solvent vapor will pour out from the nitrogen inlet if it is left open to the air. 8
Page 1 and 2: T-Squared Technology, Inc 903 Sneat
Page 3 and 4: CAUTION * The Chromatotron is to be
Page 5 and 6: Page MAINTENANCE AND REPAIRS Electr
Page 7 and 8: THE CHROMATOTRON ® U.S. Patent no.
Page 9 and 10: ___SETTING UP AND USING THE CHROMAT
Page 11: Maintain the wick in good condition
Page 15 and 16: Scraper blades are available for so
Page 17 and 18: Observe the solvent front during th
Page 19 and 20: Samples of low solubility can also
Page 21 and 22: Solvents must not absorb UV light.
Page 23 and 24: Clean-up solvent can also be remove
Page 25 and 26: ____________COATING ROTORS WITH SOR
Page 27 and 28: Recipe 1B, Silica Gel TLC Standard
Page 29 and 30: Recipe 6, Silica Gel - Glue Glue-bo
Page 31 and 32: Measure out the water, cool, in a r
Page 33 and 34: SCRAPING SORBENT LAYERS Allow oven
Page 35 and 36: Weigh out the sorbent into a 250-30
Page 37 and 38: Recipe 4, page 23, for partition la
Page 39 and 40: ADJUSTING THE MAIN VESSEL If the ro
Page 41 and 42: PROBLEM CAUSE CURE Bands separate t
Page 43 and 44: PROBLEM CAUSE CURE 4 Weak UV lamp.
Page 45 and 46: __________________CHROMATOTRON PART
Page 47 and 48: ___________________________________

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