Chromatotron

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The Rotor Support System 3
___SETTING UP AND USING THE CHROMATOTRON INSTALLATION Set up the Chromatotron in a fume hood or similar area designed for the safe handling of organic solvents. Place the Chromatotron away from hotplates, steambaths or other sources of heat. THE MAIN VESSEL The main vessel is constructed from "acetal" polymer. After completion of chromatography, wash out solvents that soften or swell acetal (chloroform, dichloromethane, acetone, acetonitrile) with hexane, other hydrocarbons or ethyl acetate. The solvents ether, ethyl acetate, tetrahydrofuran, dioxane, methanol, hydrocarbons and carbon tetrachloride are relatively inert towards acetal. Acetic acid, trimethylamine and ammonia are acceptable as additives to chromatography solvents. The possibility of solvent damage to plastic parts can be reduced by leaving the nitrogen supply on when the Chromatotron is not in use. Do not use mineral acids, formic or other acids stronger than acetic acid. Chloroform contains hydrochloric acid which will attack the main vessel, metal parts and your sample. Remove acid by adding alumina or other solid bases to the chloroform and check with wet pH paper. Consider using dichloromethane in place of chloroform. Clean the solvent collection channel at intervals and clear the output tube with a straightened paper clip. THE TEFLON LID The polyethylene cover should be placed over the Teflon lid when the Chromatotron is not in use. When the Teflon lid is out of the Chromatotron, place it on the inverted polyethylene cover: 4
Page 1 and 2: T-Squared Technology, Inc 903 Sneat
Page 3 and 4: CAUTION * The Chromatotron is to be
Page 5 and 6: Page MAINTENANCE AND REPAIRS Electr
Page 7: THE CHROMATOTRON ® U.S. Patent no.
Page 11 and 12: Maintain the wick in good condition
Page 13 and 14: Pumps are sensitive to abuse: * The
Page 15 and 16: Scraper blades are available for so
Page 17 and 18: Observe the solvent front during th
Page 19 and 20: Samples of low solubility can also
Page 21 and 22: Solvents must not absorb UV light.
Page 23 and 24: Clean-up solvent can also be remove
Page 25 and 26: ____________COATING ROTORS WITH SOR
Page 27 and 28: Recipe 1B, Silica Gel TLC Standard
Page 29 and 30: Recipe 6, Silica Gel - Glue Glue-bo
Page 31 and 32: Measure out the water, cool, in a r
Page 33 and 34: SCRAPING SORBENT LAYERS Allow oven
Page 35 and 36: Weigh out the sorbent into a 250-30
Page 37 and 38: Recipe 4, page 23, for partition la
Page 39 and 40: ADJUSTING THE MAIN VESSEL If the ro
Page 41 and 42: PROBLEM CAUSE CURE Bands separate t
Page 43 and 44: PROBLEM CAUSE CURE 4 Weak UV lamp.
Page 45 and 46: __________________CHROMATOTRON PART
Page 47 and 48: ___________________________________

Chromatotron

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