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Procedure Widely Used
in the Footwear Industry
The advantage of this challenge test method is that it
can be reproducibly performed in the laboratory, particularly
on complex composite systems and samples
that are non-planar or irregularly shaped, as is generally
the case with footwear components. Moreover, in
contrast to other test methods, no identical untreated
control material is necessary to perform testing and
evaluation of antibacterial activity. These features
have led to widespread use of the method in the footwear
industry.
Plating of the samples for enumeration
of the viable microorganisms
01.2011
Magazine of the Test and Research Institute Pirmasens
In this quantitative method, samples treated with
antibacterial agents (and where appropriate also untreated
control materials) are shaken in a bacterial suspension
with a defined microbial concentration of a
given test organism, hence the commonly used name
‘shake-flask test’. The initial microbial concentration is
first determined. After incubation under dynamic conditions
for specified periods, the viable bacteria are
enumerated and the antibacterial activity calculated.
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