Lightning–Link™ Antibody labeling kits - BioNova

Innova Biosciences 

first for Antibody labeling 

Lightning–Link Antibody labeling kits 

Lightning-Link kits 

The Lightning-Link kit allows you to label an antibody with minimal hands-on time - less than 30 

seconds. Simply add your antibody to a tube of Lightning-Link mix and the label becomes covalently 

attached to your antibody in a gentle controlled process. 

As Lightning-Link is a one-step antibody labeling procedure (i.e. is not interrupted by desalting or 

dialysis steps), your antibody can be prepared with only microgram quantities of antibody, with recoveries 

approaching 100%. Furthermore, the technology is easily scalable (μg to mg) ensuring quality 

and consistency. Thus Lightning-Link provides a simple antibody labeling method which saves both 

time and money. 

Add 

LL-Modifier 

Purified antibody 

Over 40 available labels 

including enzymes, fluorescent protein/dyes, 

tandems, biotin and avidin 

Very easy to use - One Step Format 

30 Seconds Hands on Time 

100% Antibody Recovery 

Freely Scalable 

Proven Technology 

Add antibody 

solution to 

Lightning–Link mix 

Incubate 

Add 

LL-Quencher 

3h RT 30 min. READY 

TO USE 

Labeled antibody Labeled antibody 

bioNova científica, s.l. • Tel.: 91 551 54 03 • Fax: 91 433 45 45 • e-mail: info@bionova.es • www.bionova.es

Producto Formato Referencia PV(€)* Producto Formato Referencia PV(€)* 

ENZYMES 

HRP 3 x 10µg 701-0030 180 

1 x 100µg 701-0010 213 

3 x 100µg 701-0000 402 

1mg 701-0002 648 

5 x 1mg 701-0003 2.074 

5mg 701-0004 2.014 

AP 3 x 10µg 702-0030 180 

1 x 100µg 702-0005 243 

3 x 100µg 702-0010 523 

1mg 702-0015 710 

GOx 3 x 10µg 706-0030 180 

3 x 100µg 706-0010 710 

1 x 1mg 706-0015 1.019 

FLUORESCENT PROTEINS 

R-PE 3 x 10µg 703-0030 180 

1 x 100µg 703-0005 276 

3 x 100µg 703-0010 523 

1mg 703-0015 865 

B-PE 1 x 100µg 716-0005 153 

3 x 100µg 716-0010 275 

1mg 716-0015 419 

APC 3 x 10µg 705-0030 180 

1 x 100µg 705-0005 306 

3 x 100µg 705-0010 556 

1mg 705-0015 865 

PerCP 3 x 10µg 718-0030 180 

1 x 100µg 718-0005 306 

3 x 100µg 718-0010 556 

1mg 718-0015 865 

FLUORESCENT DYES 

Fluorescein 3 reactions (each up to 20µg) 707-0030 180 

3 reactions (each up to 200µg) 707-0010 463 

1 reaction (up to 2mg) 707-0015 743 

Rhodamine 3 reactions (each up to 20µg) 710-0030 180 



AMCA 3 reactions (each up to 20µg) 712-0030 180 



*Los precios indicados no incluyen IVA ni gastos de envío 

Texas Red 3 reactions (each up to 20µg) 714-0030 180 



CY DYES 

Cy3 3 reactions (each up to 20µg) 780-0030 180 






Cy5.5 3 reactions (each up to 200µg) 782-0010 284 





Cy3.5 3 reactions (each up to 200µg) 784-0010 284 


ATTO DYES 

atto390 3 reactions (each up to 20µg) 730-0030 185 






















Producto Formato Referencia PV(€)* Producto Formato Referencia PV(€)* 

















FLUOROPROBE DYES 

547H 3 reactions (each up to 20µg) 788-0030 180 



594 3 reactions (each up to 20µg) 790-0030 180 



647H 3 reactions (each up to 20µg) 791-0030 180 









TANDEM DYES 

PE/Cy5 3 x 10µg 760-0030 189 

1 x 100µg 760-0005 306 

3 x 100µg 760-0010 648 

1 x 1mg 760-0015 926 

PE/Cy5.5 3 x 10µg 761-0030 189 

1 x 100µg 761-0005 306 

3 x 100µg 761-0010 648 

1 x 1mg 761-0015 926 

PE/Cy7 3 x 10µg 762-0030 189 

1 x 100µg 762-0005 306 

3 x 100µg 762-0010 648 

1 x 1mg 762-0015 926 

PerCP/Cy5.5 3 x 10µg 763-0030 189 

1 x 100µg 763-0005 306 

PE/Texas Red 3 x 10µg 767-0030 189 

1 x 100µg 767-0005 182 

3 x 100µg 767-0010 376 

1 x 1mg 767-0015 535 

PE/Atto594 3 x 10µg 768-0030 189 

1 x 100µg 768-0005 182 

3 x 100µg 768-0010 376 

1 x 1mg 768-0015 535 

PerCP/Cy5.5 3 x 100µg 763-0010 648 

1 x 1mg 763-0015 926 

APC/Cy5.5 3 x 10µg 764-0030 189 

1 x 100µg 764-0005 306 

3 x 100µg 764-0010 376 

1 x 1mg 764-0015 926 

APC/Cy7 3 x 10µg 765-0030 189 

1 x 100µg 765-0005 306 

3 x 100µg 765-0010 648 

1 x 1mg 765-0015 926 

BIOTIN/STREPTAVIDIN 

Biotin conjµgation 3 reactions (up to 20µg Ab) 704-0030 180 

kit type A 

3 reactions (up to 200µg Ab)704-0010 337 

1 reaction (up to 2mg Ab) 704-0015 457 

Biotin conjµgation 3 reactions (up to 20µg Ab) 715-0030 180 

kit type B 

3 reactions (up to 200µg Ab)715-0010 648 

1 reaction (up to 2mg Ab) 715-0015 802 

Streptavidin 3 x 10µg Streptavidin 708-0030 180 

3 x 100µg Streptavidin 708-0010 337 

1 x 1mg Streptavidin 708-0015 457 

Avidin 3 x 10µg Avidin 717-0030 180 

3 x 100µg Avidin 717-0010 337 

1 x 1mg Avidin 717-0015 457 

PROTEIN PURIFICATION KITS 

AbSelect Antibody 1 purification 860-0005 185 

Purification Kit 3 purifications 860-0010 423 

Antibody Concentration 1 Spin Cartridge 861-0005 32 

and Clean up kit 3 Spin cartridges 861-0010 58 

*Los precios indicados no incluyen IVA ni gastos de envío 


Lightning-Link - Frequently Asked Questions 

Antibody 

Does the antibody have to be purified? 

Yes – purified antibodies are perfect for Lightning-Link. Please note 

common additives present in buffer formulations such as BSA (up to 

1%) and gelatin (up to 0.5%) are tolerated - please see our website for 

further technical information. Antibodies which are present in ascites 

fluid, serum or hybridoma culture media need to be purified. 

What is the ideal starting concentration of the antibody? 

Antibody concentration should be in the range of 0.5-2.5mg/ml. 

Is the Lightning-Link antibody labeling chemistry isotype or species 

specific? No. 

Methodology 

Is the hands-on time really only 30 seconds? 

Yes – naturally you will need to familiarize yourself with the 

instructions before using Lightning-Link products for the first time, 

but after that your hands-on time should be no more than 30 seconds. 

How scalable is the technology? 

Scalability is one of the major advantages of Lightning-Link. Rather 

than risk valuable antibody you can label a small quantity and be 

confident that the bulk quantity made later will have essentially 

identical properties. 

Lightning-Link - Custom Antibody Labeling 

Antibody-Label Optimization (Micro-optimizer ) 

Optimization of labeled antibodies at the microgram scale. Use our 

proprietary technologies to enhance the performance of your labeled 

antibodies. By creating an array of trial conjugates (5 formulations per 

label) at low microgram scale (20μg per formulation) combined with 

selection by you in the assay of interest, the best possible conjugate 

can be obtained. 

PerfectSCALE 

All conjugates produced using our micro-optimization technology 

can be scaled over many orders of magnitude without the need for 

re-development of the process. The inherent simplicity of Innova’s 

technology means that exceptionally high yields are obtained, with 

minimal batch-to-batch variation, ensuring consistent performance 

with exceptionally high recovery. 

Antibody Labeling Chemistry 

What functional groups do I need on my antibody to use 

Lightning-Link? 

Lightning-Link requires amine groups on the molecule to be labeled. 

Most proteins or peptides have lysine and/or alpha-amino groups. All 

antibodies have multiple amine functions. 

Can proteins other than antibodies be labeled? 

Yes – while labeling of primary antibodies is a major application, the 

only requirement is that the protein or molecule to be labeled has 

amine functionality. 

Is the label attached to my protein by a covalent bond? 

Yes – conjugates formed with Lightning-Link are perfectly stable. 

Will my antibody couple to itself or form high molecular weight 

aggregates? 

No – the antibody labeling chemistry is unidirectional and therefore 

labeling will only occur between the antibody and the label. 

Antibody Buffers 

What buffer additives can be used and what should be avoided? 

Additives such as salts (e.g NaCl), sugars (e.g. sucrose) and 

chelators (e.g. EDTA) have no effect on the antibody labeling 

reaction. The main additives to avoid are amino acids (e.g. glycine), 

blockers (e.g. ethanolamine), tris buffer above 20mM and thiols 

(DTT, mercaptoethanol). 

Fast & FAb Antibody Labeling Service 

Antibody labeling service within 48 hours. Using state of the art 

labeling chemistry, we have the ability to label microgram to milligram 

amounts of your antibody with a variety of popular labels within 48 

hours. High quality labeled antibodies delivered in a timely and 

efficient manner. 

Oli-Join 

Antibody-oligonucleotide conjugates are the next generation of tools 

in biomarker detection, overcoming sensitivity and linear range issues 

commonly encountered with standard antibody labels. Through our 

Oli-Join service, we have the expertise to label an antibody directly 

to an oligonucleotide of any given sequence. Furthermore, we have the 

ability to create an oligo-antibody hybrid using a small amount of 

antibody material – as little as 100ug.

Lightning–Link™ Antibody labeling kits - BioNova

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