Molecular Devices Image Express Brochure_rev_B_LR.pdf

uspace.shef.ac.uk

Molecular Devices Image Express Brochure_rev_B_LR.pdf

now part of MDS Analytical Technologies

Cellular Imaging Solutions

ImagIng wIth a vISIon


mDS analytical technologies provides the complete workflow solution for automated

microscopy and high-content screening (hCS), facilitating image acquisition through

analysis and data management. three unique imaging systems with research-grade

optics span sub-cellular resolution to imaging of model organisms, enabling you to

address basic research through drug discovery applications. Image analysis based

on the industry-standard metamorph ® Software enhances productivity for novice and

expert users with the support of custom macros and a suite of turnkey hCS application

modules. Integrated data management simplifies storage, image analysis and hit

selection to ensure your data is ready for the next step.


a Complete Solution from Image acquisition to Data mining & hit Selection

Image Acquisition

ImageXpress Micro System

ImageXpress Ultra System

IsoCyte Cytometer

Data Management

Autonomous Mode

a turnkey solution: Image acquisition is integrated with the mDCStore Data management Solution which works seamlessly with the

image analysis and hit selection tools. In autonomous mode, the IsoCyte cytometer performs on-the-fly image acquisition and analysis.

Corporate

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ephys

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analyst

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Flex

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FlIpR

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Imaging

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Integrated

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ephys

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analyst

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Flex

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FlIpR

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lifeSci

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Imaging

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precision motion

MDCStore Data

Management Solution

a Complete anD FleXIBle aCQUISItIon

anD analYSIS platFoRm

Enjoy the benefits of a turnkey solution that

streamlines the workflow required to conduct

HCS and hit selection in a fully integrated

environment. Regardless of the MDS Analytical

Technologies imaging system used, acquired images

can be stored in the secure MDCStore Data

Management Solution and made accessible for

sophisticated analysis by MetaXpress ® Software

Application Modules or customized macros. When

incorporated with the AcuityXpress Corporate

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ephys

8181

analyst

8243

Software, data

Flex

visualization, mining and hit selection are ready 8303

to use upon system installation. This facilitated

FlIpR

workflow avoids bottlenecks in image analysis 8303and

data processing that are common to HCS. If third-

Quality optics

lifeSci

8142

Imaging

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Image Analysis

MetaXpress and

MetaXpress PowerCore Software

Hit Selection

AcuityXpress Software

party analysis tools are required, the MDCStore

Data Management Solution open application

programming interface (API) provides a portal for

data migration to external host databases or thirdparty

applications.

validated

mDS analytical technologies > 0/ 1


multiple Imaging technologies address Specific needs

thRee Image aCQUISItIon platFoRmS

get the pICtURe RIght

Choose the platform to match the image resolution

and speed required for your research and highthroughput

imaging needs. Research-quality optics

and premium hardware performance are standard,

Corporate

Corporate

whether 8222 you require widefield 8222 optics with a CCD

camera; simultaneous 4-color, confocal scanning

for ephys optical sectioning; or a telecentric ephys scan lens for

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8181

imaging thick optical sections.

Capturing low- to high-resolution is easy with the

ImageXpress ® Micro and ImageXpress Ultra Systems

Flex

Flex

8303

8303

that generate research-quality images at high speed

and can be operated with 1X to 100X objective

FlIpR

FlIpR

lenses. 8303 These systems provide the 8303 best X, Y and Z

positioning available using voice coil motor movement

lifeSci

lifeSci

with 8142 100 nm resolution to precisely 8142 locate and

ImageXpress micro widefield System

the ImageXpress micro System base unit; available options include cooled CCD and high-speed laser auto focus.

Inset: ImageXpress micro System with environmental control and fluidics (top) and transmitted light (bottom) options.

analyst

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Imaging

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Integrated

analyst

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Imaging

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precision motion

identify sub-cellular features and ensure excellent X,

Y and Z repeatability. Pinpoint positioning simplifies

multi-site time-lapse and tiling for large multi-image

experiments. Rapid positioning, in combination with

the laser autofocus option and an easy setup wizard,

furnishes users with an intuitive interface to further

Corporate

improve overall workflow.

8222

The large-format IsoCyte High-Speed ephys Laser ephys

8181

8181

Scanning Cytometer is complimentary to the

ImageXpress Microscopy Platforms, analyst providing analyst

8243

8243

versatility to the Complete Imaging Solution. The

IsoCyte System brings cellular-resolution imaging to

Flex

Flex

traditional high-throughput screening 8303 and cytometry

8303

applications, providing high-content data earlier in

FlIpR

FlIpR

the drug discovery process. Regardless 8303 of where 8303the

biology takes you, the MDS Analytical Technologies

lifeSci

lifeSci

Complete Imaging Solution grows 8142 with your 8142 needs.

Quality optics

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Imaging

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transfluor ® assay captured by the ImageXpress micro System.

three-color overlay of motor neurons in culture captured on

the ImageXpress micro System.

wIDeFIelD ImagIng

The ImageXpress Micro System is a versatile

instrument offering an inverted widefield microscope

with CCD camera options. Industry-leading stage

and focus control, coupled with a high-speed laser

autofocus option, make the ImageXpress Micro

System the fastest and most precise automated

CCD imaging station available. Combined with

mature software, the ImageXpress Micro Instrument

outperforms automated microscopes built from

off-the-shelf components. Hardware options for

environmental control and fluidics enable live cell

assays and long time-lapse experiments. The ability to

overlay fluorescence images on phase contrast images

is possible with the transmitted light option. With so

many possibilities, the ImageXpress Micro System is

able to automate any fluorescent, bright field or phase

contrast microscopy application.

validated

h i g h e r t h r o u g h p u t


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ephys

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analyst

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Flex

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ImageXpress Ultra Confocal System

Integrated

the ImageXpress Ultra System is a four-channel confocal imager

available with the following lasers: 405, 488, 532, 561, and 635 nm.

Imaging on the ImageXpress Ultra System: overlays of single

cell image (above) at 60x/1.4na oil (actin in green, nuclei in

blue, and mitochondria in red) and a long format scan of U2oS

cells from a transfluor assay (left).

ConFoCal ImagIng

The ImageXpress Ultra System is the only true pointsource,

laser scanning confocal imaging platform

configured for 4-color simultaneous HCS. Sequential

and simultaneous scanning are both possible. When

applicable, simultaneous scanning significantly improves

Corporate

acquisition 8222 speed. A configurable pinhole provides an

advantage over spinning disk confocals by allowing

fine ephys tuning of z-section thickness and signal strength

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to achieve the ideal speed and image quality for your

assay. analyst The ImageXpress Ultra System shares a small

8243

footprint, precision X, Y and Z control, and high-speed

laser autofocus technology with the ImageXpress Micro

Flex

8303 System. Unique to the ImageXpress Ultra System is the

ability to acquire continuous long scans up to a 1:32

FlIpR

aspect 8303 ratio to track long detailed cellular processes. The

ImageXpress Ultra System is the best-in-class choice for

high-speed, research-quality confocal imaging for HCS.

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Imaging

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precision motion

Quality optics

IsoCyte laser Scanning Cytometer

the IsoCyte high-Speed laser Scanning Cytometer; choose from 405, 440, 488,

532, and 640 nm excitation wavelengths.

Imaging on the IsoCyte System: Cells stained and imaged in “whole well” format

in a 96-well plate (left) can be identified, counted, and classified. whole organisms,

such as worms (c. elegans) can also be imaged (right).

hIgh-SpeeD, laRge-FoRmat ImagIng

The IsoCyte Laser Scanning Cytometer is a fast, automation-friendly

system that enables real-time imaging for cell- and bead-based assays,

array scanning, colony assays, small organisms and tissue slices. The

system’s telecentric lens captures emissions from a 400-µm thick

optical slices. This eliminates the need for focusing, and is ideal for

Corporate

large 8222cell

populations, 3-D cultures or small organisms. In addition,

high sensitivity is available in up to four channels to support

multiplexed ephys assays and maximum experiment efficiency. Scanning is

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“format-independent” and an entire plate or slide (6- to 1536-well

microplates analyst or slides) is imaged in 2–10 minutes depending on the

8243

selected image resolution. Object-based “on-the-fly” image analysis

provides immediate results. The versatile IsoCyte System bridges

Flex

the 8303 gap between HCS applications and high-throughput imaging

assays, expanding the range of high-content discovery to formats

FlIpR

beyond 8303 cellular assays. Images or data can be further analyzed with

MetaXpress and AcuityXpress Software or exported in FCS format

for plotting in any flow cytometry software.

lifeSci

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Imaging

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validated

mDS analytical technologies > 2 / 3


Flexible Image acquisition and analysis Software

the metaXpress Software interface showing a well-based gallery (right) and individual images from a transfluor assay in their

raw (right) and analyzed (middle) states.

IntegRateD woRkFlow envIRonment

MetaXpress Software is built upon the industrystandard

MetaMorph Cellular Imaging

Software and is the common platform for image

acquisition, image analysis and automation for

the ImageXpress Micro and Ultra High-Content

Corporate

Corporate

Imaging 8222 Instruments. Leveraging 8222 the legacy of

MetaMorph Software, MetaXpress Software

ephys

ephys

incorporates the same techniques refined by the

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8181

demands of cell biology research for over 20 years.

MetaXpress analyst software includes analyst powerful tools

8243

8243

specifically designed to facilitate configuration

of image acquisition and image analysis for any

Flex

Flex

8303

8303

HCS workflow. A single interface for configuring

plate types, image capture protocols and triggering

FlIpR

FlIpR

automated 8303 image analysis is intuitive 8303 and easy to

learn. Once configured, walk-away automation

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Integrated

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precision motion

and analysis can even be triggered for multiple

instruments.

Enjoy the vast capabilities developed for research

imaging with enhancements that greatly simplify

Corporate Corporate

high-content screening. MetaXpress Software

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provides users with proven automation tools to

configure image acquisition and analysis ephys routines ephys

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8181

so you can walk away from the system for extend

durations. These include a collection analystof

optional analyst

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8243

application-specific software modules that facilitate

common assays and high-speed image analysis

Flex

Flex

options. If you have experience with 8303 MetaMorph 8303

Software, learning to navigate the MetaXpress

FlIpR

FlIpR

Software user interface is intuitive 8303 and powerful. 8303

All of the MetaMorph Software capabilities are

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analysis of poly- and micro-nucleated cells (top) and neurite

outgrowth (bottom) with dedicated metaXpress Software application

modules showing captured image (left) and processed image (right).

also available for advanced or custom applications.

Custom acquisition and analysis macros can be

integrated to enhance HCS protocols. MetaXpress

Software provides integration with the MDCStore

Data Management Solution and facilitates

processing and reprocessing of large datasets

and maintains workflow. Instrument settings,

analysis configurations, images and analyzed data

are seamlessly managed in a database to ensure a

smooth workflow. Training courses on MetaXpress

Software are available to provide novice and expert

users with knowledge to maximize the potential of

all of their cellular and imaging assays.

validated

h i g h e r t h r o u g h p u t


easy modules enable hundreds of assays

Cell Proliferation

Count Nuclei

Cell Viability

Live/Dead

Membrane

Potential

Cell Health

Fatty Acid Uptake

Cell Scoring

Pathway Analysis

and Multiplexing

Multi-Wavelength Cell Scoring

Toxicology/Genotoxicity

Micronuclei

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ephys

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analyst

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Flex

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Mitochondrial

Localization

Translocation

FlIpR

FlIpR

the 8303 adaptive Background Correction feature 8303 in every metaXpress Software application module

compensates for unexpected artifacts in the captured image (left) to produce robust and accurate

segmentation lifeSci (right). In this example lifeSci nuclear identification is robust even with debris in the image.

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Imaging

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Integrated

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analyst

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precision motion

Process Extension

Neurite Outgrowth

Intracellular

Trafficking

Multi-Wavelength

Translocation

Quality optics

Receptor Internalization and

Punctate Patterns

Granularity, Transfluor ® Assay

Cell Cycle

Mitotic Index, Cell Cycle,

Monopole Detection

Protein Expression

& Modification,

Transient Transfection

Cell Scoring

Cell Signalling

by Translocation

Translocation

(Enhanced)

Tube Formation

Angiogenesis

applICatIon moDUleS

A full suite of MetaXpress Software Application Modules is

available for turnkey image segmentation and quantification

tasks required by common high-content screening assays,

from cell counting to intracellular signalling to cell health to

micronuclei identification. Special knowledge in microscopy,

Corporate

image 8222 analysis or cell biology is not required, and there is no

scripting or code writing involved. Simply set the values that

ephys identify features of interest, preview to confirm and run a batch

8181

analysis. Reliable, reproducible results are generated using

proprietary analyst algorithms that are built into all of the modules and

8243

require minimal training. In addition, the Adaptive Background

Correction process improves image segmentation and object

Flex

8303

identification for automated microscopy. The power of the

MetaMorph Software backbone compliments the turnkey

FlIpR

application 8303 modules for novel or custom work where user-

configured macros can be put to use with ease.

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validated

Legend

Applications

Application Modules

mDS analytical technologies > 4/ 5


Integrated Data management Solution and high-Speed Image processing

FleXIBle anD SeCURe Data management

Flexibility and integrity are crucial aspects of

data management in any automated imaging or

screening environment. The MDCStore Data

Management Solution is an open, commercially

supported platform for managing imaging data,

Corporate

Corporate

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8222

providing users with a validated way to securely

access, manage and deploy their automated

ephys

ephys

imaging 8181 and HCS. An open API 8181 is available for

integration with host databases or third-party

analyst

analyst

tools. 8243 The API can also be used 8243 for development

of applications for non-commercial purposes.

Flex

8303

FlIpR

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lifeSci

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Image analysis the power of parallel processing

metaXpress

Software

Integrated

mDCStore

Data management Solution

Flex

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FlIpR

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lifeSci

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precision motion

Process 1 Process 2 Process 3 Process 4 Process 5 Process 6

Client 1 Client 2 Client 3

metaXpress powerCore

Software

hIgh-thRoUghpUt Image pRoCeSSIng

MetaXpress PowerCore Software is a distributed

analysis platform for MetaXpress Software

Application Modules, providing revolutionary

improvements in analysis speed. The same highquality

image analysis algorithms applied by the

Corporate Corporate

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8222

application modules are now scalable to ensure

analysis times are as fast or faster than image

ephys

ephys

acquisition times. Throughput is 8181 maximized 8181by

simultaneously distributing images to multiple

analyst

analyst

CPUs or processor cores to execute 8243 analysis. 8243

With processing speed increased by 5- to 40-fold,

image analysis bottlenecks are eliminated Flex and Flex

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8303

do not interfere with optimal image acquisition

performance. Using MetaXpress FlIpR PowerCore FlIpR

8303

8303

Software, multiple ImageXpress Systems can be

run in parallel and large datasets can be re-analyzed

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Quality optics

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Server

metaXpress powerCore Software accelerates

the hCS workflow by significantly reducing

time required for image analysis. leveraging

the power of parallel processing, metaXpress

powerCore Software is a server-client solution

that enables users to leverage a scalable number

of client based processors to run the metaXpress

Software application module algorithms in parallel,

accelerating analysis 5- to 40-fold.

with no image analysis bottlenecks. Highthroughput

imaging is now possible. Ultimately

your data and hit quality depend largely on image

analysis quality. Get it right and get results fast

with MetaXpress Software Application Modules

and the MetaXpress PowerCore Software option.

validated

h i g h e r t h r o u g h p u t


A

B

C

Integrated high-Content analysis: visualize Your Data

CellUlaR InFoRmatICS

AcuityXpress Cellular Informatics Software is

specifically designed to address the large, multiparametric

datasets generated by high-content data

analysis. Data are smoothly exchanged between

AcuityXpress Software and the MDCStore Data

Corporate

Corporate

8222

8222

Management Solution to ensure data analysis is

performed efficiently and that all additional content

ephys

ephys

is funneled 8181 back into one central 8181 storage location.

analyst

analyst

The 8243 interactive image viewer in 8243AcuityXpress

Software brings visualization of high-content data

to the Flex next level. This unique Flex and ground-breaking

8303

8303

tool allows for direct interaction between images

and FlIpR numerical data at all levels FlIpR at any time. Drill

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8303

down from plots, clusters and dose-response curves

to the original high-resolution images to validate

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Integrated

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D

precision motion

acuityXpress Software provides a number of different views of your

dataset. all views are interactive and linked to the original images.

a: multi-parametric profiling; B: analysis of dose-response using curvefitting

(left) or multi-parametric analysis (right); C: analysis 2-D scatter

plots; D: 3-D scatter plot of principal components analysis results.

the results and confirm hits. Multi-color overlays

and image segmentation can be viewed during

drill down to validate all data points necessary

to make an informed decision. Once hits are

identified, they can be reviewed at the single-plate

Corporate

8222

level or across multiple screens. The integrated

AcuityXpress Software streamlines your workflow,

ephys

making data processing and hit profiling easier 8181

than ever. Additional features include:

analyst

> Curve-fitting for IC50 and EC50 calculation 8243

with predefined and custom functions.

Flex

> Multiple search, minimization and weighting

8303

methods, parameter seeding and model

comparison are available for the very best FlIpRfits.

Quality optics

8303

lifeSci

8142

Imaging

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the interactive image viewer brings

visualization of high-content data to the

next level by allowing direct interaction

between your images and numerical data

visualizations at all levels. navigate through

data and images in multiple ways, from

the detailed view of the individual highresolution

images to the global, high-level

view of all of the image data in a large

gallery. Detailed multi-color views of the

original images can be seen with the image

segmentation overlay to confirm proper

image segmentation was performed.

> A number of advanced multi-parametric

analysis tools for data mining: K-means,

K-medians, hierarchical clustering, selforganizing

maps, principal component analysis

and more—using cellular phenotypes and

compound responses.

> The interactive image viewer makes it possible

to select the image data to analyze specific

populations of cells or compounds.

> Statistical data include Z'-factor, p-values

correlation coefficients.

validated

mDS analytical technologies > 6/ 7


ordering Information

ImageXpReSS mICRo BaSe SYStem

> ImageXpress Micro Widefield System

> MetaXpress Software

> MDCStore Data Management Solution

> PC with Windows ® XP Operating System

> 1-year warranty (after installation)

Part Number: IXM IMAGING MSCOPE

ImageXpReSS mICRo SYStem optIonS

CoolSNAP HQ Cooled CCD Camera

Part Number: 1-2300-1060

Laser autofocus

Part Number: 1-2300-1037

CRS CataLyst Express Plate Loading

Robot option for ImageXpress Micro System

Part Number: 1-2300-1084

Transmitted light (120V or 240V)

Part Number: 1-2300-1076

Environmental control

120V: Part Number: 1-2300-1113

240V: Part Number: 1-2300-1114

Transmitted light/environmental control

120V: Part Number: 1-2300-1115

240V: Part Number: 1-2300-1116

Fluidics/environmental control

120V: Part Number: 0310-5079

240V: Part Number: 0310-5080

Corporate

8222

ephys

8181

analyst

8243

Flex

8303

FlIpR

8303

lifeSci

8142

Imaging

8263

Integrated

Corporate

8222

ephys

8181

analyst

8243

Flex

8303

FlIpR

8303

lifeSci

8142

Imaging

8263

precision motion

ImageXpReSS UltRa BaSe SYStem

> ImageXpress Ultra Confocal System

> MetaXpress Software

> MDCStore Data Management Solution

> Laser options (up to 4): 405, 488, 532

or 561 and 635 nm

> Computer with Windows XP Operating

System

> 1-year warranty (after installation)

Part Number: IMAGEXPRESS ULTRA

ImageXpReSS UltRa SYStem optIonS

CRS CataLyst Express Plate Loading Robot

option for ImageXpress Ultra System

Part Number: 0310-5355

ISoCYte SYStem

> IsoCyte Laser Scanning Cytometer

> PC with Windows XP operating system

> 1-year warranty (after installation)

> Laser options (up to 2): 405, 440, 488,

532, or 640 nm

ISoCYte SYStem optIonS

SynchroMax Robotic Plate Handling

System option for IsoCyte System

Part Number: 0200-6234

additional options are available to customize your systems,

including objectives, filter sets, software and application

modules. please contact your local sales representative for

more information.

Corporate

8222

ephys

8181

analyst

8243

Flex

8303

FlIpR

8303

lifeSci

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Quality optics

Imaging

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Corporate

8222

ephys

8181

analyst

8243

Flex

8303

FlIpR

8303

lifeSci

8142

Imaging

8263

SoFtwaRe optIonS

AcuityXpress Cellular Informatics Software

Part Number: 9500-0105

MetaXpress Offline Analysis Software with

MDCStore Integrated Data Management

Utility

Part Number: METAX OFFLINE

MetaXpress PowerCore Offline Analysis

Station with MDCStore Integrated Data

Management Utility

Part Number: MX POWERCORE

validated

h i g h e r t h r o u g h p u t


now part of MDS Analytical Technologies

SaleS oFFICeS

United States & Canada

mDS analytical technologies

tel. +1-800-635-5577

Fax +1-408-747-3601

Brazil

molecular Devices Brazil

tel. +55-11-3616-6607

Fax +55-11-3616-6607

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molecular Devices Beijing

tel. +86-10-6410-8669

Fax +86-10-6410-8601

molecular Devices Shanghai

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Fax +86-21-6887-8890

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mDS analytical technologies gmbh

tel. +49-89-9605-880

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Fax +82-2-3471-9532

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mDS analytical technologies (gB) ltd.

tel. +44-118-944-8000

Fax +44-118-944-8001

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FoR ReSeaRCh USe onlY. not FoR USe In DIagnoStIC pRoCeDUReS.

wInDowS is a registered trademark of microsoft Corporation. CoolSnap is a trademark of

Roper Industries, Inc. CatalYSt eXpReSS is a trademark of thermo Fisher Scientific, Inc.

ImageXpReSS, metamoRph, metaXpReSS, moleCUlaR DevICeS, and tRanSFlUoR are

registered trademarks and aCUItYXpReSS, ISoCYte, mDCStoRe, and SYnChRomaX are

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respective owners. Specifications subject to change without notice.

©2009 mDS analytical technologies (US) Inc. printed in U.S.a. 10/09 #0120-1490.B

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