sbornik_s_2011.pdf

XI. Woorkshop
of Physsical
Chemists and a Electrochemmists´11
A
C
Fig. 3: Transveersal
sectio ons of petiole
(A) an nd lamina (B – D): ggeneral
ana atomy (A), ,
magnnification
xx100
and x2 200 (B, C), aand
detailed
structure lamina (D) ), magnification
x200. .
Acriddine
orangge
staining using FITCC
(A, C) an nd DAPI fi ilters (B, DD).
Descript tion A: a –
epideermis,
b – cuticle, c – hypoderrmis,
d – ground g tissu ue - parencchyma,
e – sclerifiedd
interrfascicular
pparenchym
ma, f – scleri rified protop phloem, g – metaphlooem,
h – me etaxylem, i
– prootoxylem,
j – central cavity – iintercellula
ar space; B: a – cuticlle,
b – epid dermis, c –
palissade
parencchyma,
d – epidermiss,
e – inter rcellular spa aces of spoongy
parenchyma,
f –
sponngy
parenchhyma
cells; C: a – guarrd
cells, b – spongy pa arenchyma cells, c – ep pidermis, d
– coollateral
vaascular
bun ndle, e – pparenchym
ma cells sur rrounding vascular bundle, b f –
epideermis
withh
cuticle, g – palisadde
parench hyma; D. a – palisadde
parench hyma, b –
epideermis,
c – collateral vascular bbundle,
d – parenchy yma cells surroundin ng vascularr
bunddle,
e – sppongy
pare enchyma, f – epider rmis, g – intercellullar
spaces of spongyy
parennchyma,
h – mechanical
tissue – collenchym ma, I - epid dermis.
4. CONCLUUSION
Work deemonstrated
d anatomyy
roots, ste ems and le eaves of ppharmaceut
tically andd
toxiccologically
important plant Aconnitum
nape ellus L. em. Skalický (RRanunculac
aceae) usingg
methhods
of lighht
and fluo orescence mmicroscopy
y. Work sho ows not onnly
to the anatomicall
strucctures
of inndividual
plant
organss,
but also to the possibility
of uusage
of flu uorescencee
micrroscopy
in tthese
types of studies.
5. ACKNOWWLEDGEM
MENT
The workk
has been n supporteed
by REM MEDTECH GA ČR 5522/10/P61
18 a FRVŠŠ
25066/F4/2011/VVFU
- 29 -
B
D
Brnoo