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XI. Workshop of Physical Chemists and Electrochemists´11 Brno in photobiological experiments evidenced the increase in the sensitivity of all tested cell models on investigated derivatives. Leukemia L1210 cells were the most sensitive to the effect of non-photoactivated and photoactivated 4-anilinoquinazolines. In conclusion our investigations show that 4-anilinoquinazolines behave as photosensitive compounds, with potential application as photosensitizers. 5. ACKNOWLEDGEMENT This study was financially supported by Scientific Grant Agency (VEGA Project 1/0018/09) and Research and Development Agency (contract No. APVV-0339-10). 6. REFERENCES [1] Sun H.-Y., Guan S., Bi H.-C., Su Q.-B., Huang W.-L., Chowbay B., Huang M., Chen X., Li C.-G., Zhou S.- F.: Journal of Chromatography B, 854 (2007), 320-327 [2] Stolze K., Udilova N., Nohl H.: Biological Chemistry, 383 (2002), 813-820 [3] Zang L., van Kuijk F., Misra B., Misra H.: Biochemistry and Molecular Biology International, 37 (1995), 283-293 - 45 -
XI. Workshop of Physical Chemists and Electrochemists´11 Brno ELECTROCHEMICAL DETECTION OF RNA USING A COMPLEX OF SIX-VALENT OSMIUM Martin BARTOŠÍK 1 , Mojmír TREFULKA 1 , Emil PALEČEK 1 1 Institute of Biophysics, Academy of Sciences of the Czech Republic, v.v.i., Královopolská 135, 612 65 Brno, Czech Republic Abstract Currently, nucleic acid electrochemistry is mainly focused on development of DNA hybridization biosensors. After a discovery that short, gene-regulating RNA molecules - microRNAs (miRNAs) - might play a role in biomedicine, particularly in cancer development, new assays for the miRNA detection have been sought. We have previously demonstrated that electroactive complexes of six-valent osmium with nitrogenous ligands (Os(VI)L) preferentially bind to polysaccharides. Recently, Os(VI)L was shown to bind also to ribose-containing oligonucleotides (ONs), if the ribose was located at the 3’-end, but not to deoxyribose. Such Os(VI)L-ON adducts were analyzed at carbon and mercury electrodes (HgE), including solid amalgam electrodes, and were easily discriminated from oligodeoxynucleotides. Moreover, application of HgE enabled picomolar determination of ribose-containing ONs due to an electrocatalytic nature of the signal provided by the adduct. This finding might lead to a new, sensitive method for miRNA detection. 1. INTRODUCTION Nucleic acids (NAs) are electroactive species undergoing oxidation and reduction processes at carbon and mercury electrodes, producing analytically useful electrochemical (EC) signals [1]. Moreover, NAs can be labeled to render them electroactive at electrodes, if (a) they do not produce any oxidation or reduction signals or (b) to discriminate between different NAs, e.g. unlabeled probe and target DNA. The fifty years history of NA electrochemistry was recently reviewed [2]. In the first three decades, only small number of laboratories studied NAs from EC point of view. However, fast progress in genomics strongly influenced the NA electrochemistry and particularly the research and development of the EC DNA sensors, making it a booming field with > 700 papers in 2010. Although EC determination of nucleotide sequences or DNA point mutations (single-base mismatches) after PCR amplification is well-established, detection of a specific (nonrepetitive), amplification-free sequence in eukaryotic genomes, as well as EC sensing of DNA-protein interactions still represent important challenges. Recently it was shown that short microRNAs (miRNA) play significant roles in molecular biology processes. For instance, miRNA was shown to be potential cancer biomarker, but its role is being investigated also in other diseases. Literature dealing with an EC analysis of RNA, as compared to DNA hybridization sensors, is still rather scarce [3, 4]. - 46 -
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• • • • • • AKCE KINETE
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NABÍDKA SPEKTROFOTOMETRŮ Dvoupapr
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Metrohm Česká republika s.r.o. Na
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VÝROBKY NEJVYŠŠÍ KVALITY Mrazí
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