4 years ago



102 Electron tomographic

102 Electron tomographic reconstruction allowed visualizing the morphology of the gold and silver NP in three dimensions. Tilt series of micrographs were recorded semi-automatically assisted by Explore 3D (FEI). These were aligned and reconstructed using Inspect 3D (FEI). Reconstructions were visualized using AMIRA (Mercury). Artefacts inherent to the analysis of projections of NPs were detected and interpreted. An estimation of the surface area, volume (Figure C) and volume specific surface area of particles in suspension was obtained and a correlation with the area and volume calculated from the equivalent circle diameter of projected NPs was demonstrated. A B C Surface area: 8592 nm² Volume: 26311 nm³ Figure (A) Micrograph showing various shapes of silver NP. Bar 50 nm; (B) Annotated micrograph showing a mixture of 4 nm silver NP (red) and 20 nm silver NP (green). Bar 100 nm; (C) Electron tomographic reconstruction of gold NP. Bar 20 nm.

Nano enhanced biosensors: the next generation of food safety diagnostics Jeroen Pollet, Filip Delport, Karel Knez, Dinh Tran, Kris Janssen & Jeroen Lammertyn BIOSYST-MeBioS, Katholieke Universiteit Leuven, W. de Croylaan 42, 3001 Leuven E-mail: Following several national and international food crises, the customer awareness for food quality and food safety is growing. Driven by public opinion and growing regulations, producers search to control the origin and quality of all ingredients and the end product. An ever increasing challenge for the food industry with both social and economic impact is food allergy detection. Accurate and reliable product information is essential to inform a rising number of food allergic patients. This has created a growing need for reliable and accurate diagnostics, operable in the complex matrix of food. In the last years, a remarkable progress has been witnessed in the development of biosensors and their applications in areas such as medical diagnostics, drug screening, environmental monitoring, biotechnology, food safety and security. Optical affinity biosensors based on surface plasmon resonance (SPR) present one of the most advanced biosensor technologies. Their ability to monitor the interaction between molecules immobilized to the surface of the sensor and molecules in solution have made SPR sensors a very powerful tool for biomolecular interaction analysis. Recently, the MeBioS-Biosensor research group ( of the K.U.Leuven has developed an innovative technology based on fibre optic surface plasmon resonance. This biosensor has a great potential as reusable, cost-effective and label free biosensor for measuring antibody-antigen, DNA hybridization and DNA-protein interactions as has been demonstrated in a recently published paper (Pollet et al., 2009). 103

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