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NANOTECHNOLOGY IN THE FOOD CHAIN - Favv

NANOTECHNOLOGY IN THE FOOD CHAIN - Favv

116 research is carried

116 research is carried out on the deposition of zinc oxide nanolayers on these biodegradable plastics, such as poly(hydroxybutyratehydroxyvalerate) (PHBV). In the presented study, the possibilities of sol-gel and sputter deposition are evaluated and discussed. Acknowledgments _________________________________________ An Hardy is a postdoctoral fellow of the F.W.O.-Vlaanderen

Identification of immune-related gene markers following interaction of engineered nanoparticles with human intestinal epithelial cells Sandra Verstraelen 1 , Patrick De Boever 1 , Eudald Casals 2 , Victor F. Puntes 2 , Hilda Witters 1 , Inge Nelissen 1 1 VITO N.V. (Flemish Institute for Technological Research), Environmental Risk and Health Unit, Mol, Belgium 2 Institut Català de Nanotecnologia (ICN), Barcelona, Spain E-mail: inge.nelissen@vito.be Nanotechnologies offer a variety of possibilities for application in the food domain, such as in food additives, nutritional supplements, packaging, and food storage devices. Besides ingestion, unintended human exposure to nanoparticles (NPs) may occur when NPs are released to the atmosphere during industrial production processes, or in derivates of agricultural products discharged to waste waters or soils. Ingestion of NPs may pose human health risks, but data on oral exposure to specific NPs and any consequent toxicity are scarce, and the implicated biological and molecular processes are largely unexplored. In this study, the human adenocarcinoma Caco-2 cell line, widely used as an in vitro model of the intestinal barrier, was exposed to suspensions of monodispersed, spherical cobalt (7 nm) and cerium dioxide (4 nm) NPs at noncytotoxic concentrations. A genome-wide transcriptomics study was performed to reveal the genes and processes involved in immune-related effects after NP exposure. Parallel experiments were set up involving cobalt chloride and cerium nitrate exposures to correct NP-specific responses by subtracting those induced by the corresponding ions. Statistically significant changes in gene expression as compared to solvent-treated cells (mean |fold-change|>1.5 (n=3), p

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