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NANOTECHNOLOGY IN THE FOOD CHAIN - Favv

NANOTECHNOLOGY IN THE FOOD CHAIN - Favv

Microarray analysis of

Microarray analysis of effects of silver nanoparticles on an in vitro translocation model of the human intestinal epithelium Hans Bouwmeester, Jenneke Poortman, Elly Wijma, P. Kinarsashanti, Ruud Peters, Ad Peijnenburg & Peter Hendriksen Wageningen University and Research Center; RIKILT- Institute of Food Safety, Wageningen, The Netherlands Email: hans.bouwmeester@wur.nl Introduction Engineered nanoparticles are increasingly being used in various food and food derived applications (Chaudhry et al., 2008). The most prominent use is in food packaging materials to increase the barrier properties, to incorporate smart sensors or as a coating with antimicrobial properties. Silver nanoparticles are used most frequently for this purpose. At present, there are a number of knowledge gaps in regard to the ADME properties and effects of nanoparticles (Bouwmeester et al., 2009). Therefore, we employed an in vitro model of the human follicle-associated epithelium to study silver nanoparticle translocation and local effects exerted by these nanoparticles. In vitro model In short, Caco-2 cells are co-cultured in a transwell design with Raij B lymphocytes resulting in a co-culture of Caco-2 cells and differentiated M-cells, specialised in particle translocation (des Rieux et al., 2007). Presence of M-cells has been confirmed immunohistochemically by using the M-cell specific marker galactin-9. Translocation studies can only be performed reliably if the integrity of monolayer of cells in the transwell design has been confirmed. For this we used Trans Epithelial Resistance as the first measure. In addition, exposure to Lucifer Yellow confirmed the integrity of the monolayer because of absence of 67

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