Views
4 years ago

NRL Proficiency Study Report

NRL Proficiency Study Report

5.2. Sulfonamides Five

5.2. Sulfonamides Five laboratories participated in the proficiency test for sulfonamide analysis (Table 6). Two laboratories (Lab A and E) performed a screening, while three confirmed the samples (Lab B, C and F) (Table 4). Laboratories B and C performed both a screening and a confirmation. A fast and simple extraction method with acetonitrile was performed by laboratory A and C to screen the samples. Afterwards, a more extensively clean-up by a liquid-liquid extraction with water and methyl tertiary butyl ether was performed to confirm the positively screened samples by participant C. Participant F used a combination with dichloromethane and acetone, followed by an SPE extraction for confirmation. Laboratory B used a combination of microbiological and receptor assay for screening. For their confirmatory purposes, an acetonitrile extraction was performed. The Premi-test ® was used by laboratory E. Table 6. Summary of the used methods for sulfonamide analysis Lab Analyis IS Technique Extraction/purification A Screening --- B C Screening --- UPLC- MS/MS Premi-test ® Charm II ® Test Confirmation Sulfachloropyridazine LC-MS/MS Screening --- LC-MS/MS Confirmation Sulfadimidine-d4 LC-MS/MS 10 ACN extraction, Centrifugation and Concentration Solvent extraction Protocol Charm – Sciences Sodium sulphate drying ACN extraction Centrifugation and Concentration ACN extraction and direct injection LLE with water and D Did not submit their results methyl tertiary butyl ether CCα µg kg -1 CCβ µg kg -1 --- 25* --- --- 40 --- LOD 5 < 50 < 50 122 133 E Screening --- Premi-test ® --- --- --- F Confirmation * CCβ of trimethoprim Sulfadiazine-C13 Sulfadimidine-C13 Sulfanilamide-C13 Sulfadimethoxine-D6 LC-MS/MS Extraction dichloromethane/acetone SPE (SCX, Varian ® ) 113 126

6. Results 6.1. Macrolides For macrolide analysis, three laboratories performed a screening, i.e. A, B and E. While laboratory F confirmed the macrolides. Results are summarized in Table 7. Lab code A B Table 7. Results for the macrolide analysis reported by the participants Sample Code SM.2010-A-1 SM.2010-A-2 SM.2010-A-3 SM.2010-A-4 SM.2010-A-5 SM.2010-B-1 SM.2010-B-2 SM.2010-B-3 SM.2010-B-4 SM.2010-B-5 Reported result Compliant Non-compliant for tylosin Compliant Non-compliant for tylosin Compliant Suspect for Tylosin (Spiramycine) Nihil Suspect for Tylosin (Spiramycine) Nihil Nihil C Did not participate in the macrolide analysis D Did not submit their results E F SM.2010-E-1 SM.2010-E-2 SM.2010-E-3 SM.2010-E-4 SM.2010-E-5 SM.2010-F-1 SM.2010-F-2 SM.2010-F-3 SM.2010-F-4 SM.2010-F-5 Suspect Suspect Compliant Suspect Suspect Compliant for macrolides 113.3 µg kg -1 tylosine 114.1 µg kg -1 tylosine Compliant for macrolides Compliant for macrolides 11 Expected results No macrolides Containing macrolides (tylosin) No macrolides Containing macrolides (tylosin) No macrolides Containing macrolides (tylosin) No macrolides Containing macrolides (tylosin) No macrolides No macrolides Containing macrolides (tylosin) Containing macrolides (tylosin) No macrolides No macrolides No macrolides No macrolides Containing macrolides (tylosin) Containing macrolides (tylosin) No macrolides No macrolides All laboratories have found the suspected samples for macrolides. No other compound(s) has (have) been reported (false positive). Moreover, all laboratories have identified the blank sample as compliant. Only one laboratory performed a screening by UPLC-MS/MS after a rapid extraction with acetonitrile (labo A). This participant identified tylosin as unknown macrolide.

ANNUAL REPORT 2010 - NRL
INV011 - Lodi - Welcome to the IAEA Proficiency Test Reporting ...
LIMITED ENGLISH PROFICIENCY (LEP) REPORTING - wveis
The Proficiency Illusion Full Report - Data Center
ANNUAL REPORT - United States Studies Centre
Adelaide Rail Freight Movements Study Final Report June 2010
What are the contents included in a project feasibility study report?
Wempa report 03 - VU University, Institute for Environmental Studies ...
New report to study the Global Dimethyl Ether Market share, size 2021
MBELI BAI STUDY ANNUAL REPORT 2008
Best [PDF] TExES Bilingual Target Language Proficiency Test (Btlpt) - Spanish (190) Secrets Study Guide: TExES Test Review for the Texas Examinations of Educator Standards Best Sellers Rank : #1 all formats#D#
World Fiber Cement Cladding Panels Market Study – 2017 Research 2022 Forecasts Report
What are the methods of project feasibility study report?
How to write Investment Decision Feasibility Study Report?
Western Area Power AdministratIon Operations Study Report
I-65 Corridor Feasibility Study Final Report - International Bridge ...
World Plating Lines Market Study – 2017 Research 2022 Forecasts Report
Botany and Wetlands Study Report - McMillen, LLC
09ANNUAL REPORT - Centre for Crime and Justice Studies
Adelaide Rail Freight Movements Study Final Report June 2010
What are the methods of project feasibility study report?
How to write Investment Decision Feasibility Study Report?
United States Anatomopathology Laboratory Cabinets Industry 2016 Market Research Report
Wempa report 04 - VU University, Institute for Environmental Studies
OCI Tracker Benchmark Study ‘Deep Dive’ Analysis Report
New report to study the Global Microcrystalline Cellulose (MCC) Market share, size 2021
World Power Module Packaging Market Study – 2017 Research 2022 Forecasts Report
World Surf Waterproof Earphones Market Study – 2017 Research 2022 Forecasts Report
World Skin Gelatin Market Study – 2017 Research 2022 Forecasts Report
buford highway transportation study: final report - Suwanee, Georgia