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4 years ago

1. Dear Laboratories, Dear Colleagues, We hereby send you ... - Favv

1. Dear Laboratories, Dear Colleagues, We hereby send you ... - Favv

1. Dear Laboratories, Dear Colleagues, We hereby send you ... -

Food pathogens NRL Food microbiology date : 8 June 2011 your references. : our references : annex(es) : SUBJECT : Version 2 of 8 June 2011, method for the detection and the isolation of EHEC O104 Dear Laboratories, Dear Colleagues, We hereby send you an update of the detection and isolation method for the pathogenic EHEC strain E. coli O104:H4 now circulating in Europe. Amendments and further details are yellow highlighted. This EHEC strain has the following characteristics, as established by the Robert Koch Institute in Germany : E.coli O104:H4, positive for vtx2+ but negative for vtx1, eae (intimin), and enterohemolysin. The strain is multiresistant to the following antibiotics : Ampicillin, Cefoxitin, Cefotaxime, Ceftazidime, Streptomycin, Tetracyclin, Trimethoprim/Sulfamethoxazole, Nalidixine acid. It produces the ESBL CTX-M enzyme (Group 1) and this characteristic may be used for the purpose of isolating the strain. As NRL we contacted the EU-RL VTEC which is now engaged in the validation process of a detection and isolation method on the basis of the procedure described in ISO TS 13136. As NRL we developed two methods for the detection and the isolation of the specific EHEC strain (see flowchart in Annex). Wetenschap ten dienste van Volksgezondheid, Veiligheid van de Voedselketen en Leefmilieu. 1. Juliette Wytsmanstraat 14 1050 Brussel | België T + 32 2 642 51 11 | F + 32 2 642 50 01. info@wiv-isp.be | www.wiv-isp.be. Aan Brigitte Pochet FAVV DG Laboratoria Kruidtuinlaan 55 1000 Brussel contact : NRL Food microbiology phone : + 32 2 642 51 83 fax : + 32 2 642 5240 email :

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