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4 years ago

Hans Bouwmeester Symposium Nanotechnology in the Food ... - Favv

Hans Bouwmeester Symposium Nanotechnology in the Food ... - Favv

Analytical methods:

Analytical methods: characterisation in matrix! HDC HDC-ICPMS HDC ICPMS ICPMS: Hydrodynamic chromatography – Inductively coupled plasma mass spectrometry Single Single Single Particle Particle-ICPMS Particle ICPMS respeonse (counts) 120000 100000 80000 60000 40000 20000 0 0 483 966 1449 1932 2415 2898 3381 3864 4347 4830 5313 scan # (ms) TEM TEM and and SEM: SEM: Transmission and Scanning Electron Microscopy 5796 6279 6762 7245 7728 8211 8694 9177 9660 Relative % 100 75 50 25 0 0 20 40 60 80 100 120 140 Particle diameter (nm)

In vitro Translocation: Study design Characterisation of Ag nanoparticles Establish non-cytotoxicitic doses for translocation studies WST-1 4 sizes of Ag particles, supernatant and AgNo 3 as control groups Assess potential translocation From: Des Rieux et al., 2007.

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