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Results 20 table 1 and

Results 20 table 1 and indicate a higher calcium agglomeration within normoxic differentiated cells. Fig. 4: Bright field confocal microscopy images of osteogenic differentiated hMSCs under normoxic (A) and hypoxic (B) conditions. Standard cell culture media served as a control (C & D). White arrows indicate condensed calcium depositions. Bar: 50µm.

Results 21 Fig. 5: Topographical confocal white light microscopy images of osteogenic differentiated hMSCs. Calcium depositions within the differentiated groups were higher than mean ECM height (A: normoxia, B: hypoxia). Control groups had constantly less ECM height. White arrows indicate condensed calcium depositions. Bar: 50µm. Alizarin Red (AR) quantification of normoxic or hypoxic osteogenic differentiated hMSCs is shown in figure 6. Normoxic induced wells had a mean AR concentration of 0.99 ± 0.25 mM. Hypoxic culture conditions did not lead to significant changes (1.11 ± 0.22 mM, p = 0.3819). The pair of controls produced a signal close to zero (Normoxia: 0.016 ± 0.005 mM, Hypoxia: 0.018 ± 0.004 mM). The comparison of osteogenic induced hMSCs against non-induced cells revealed highly significant differences for both culture conditions (p ≤ 0.001).

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