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Results 34 On ionized

Results 34 On ionized polystyrene surfaces (NUNC) normoxic hMSCs showed a mean cell volume of 691.20 ± 1001.53 µm 3 , whereas hypoxic cells had a volume of 530.04 ± 427.62 µm 3 . Coating with collagen I, fibronectin or laminin decreased cell volume and statistical spreading in both culture settings. Collagen I coating produced a cell volume of 417.56 ± 256.77 µm 3 for normoxic cells and 302.24 ± 151.88 µm 3 for hypoxic cultured cells. Fibronectin coating ended in a cell volume of 539.00 ± 217.76 µm 3 (Normoxia) and 358.72 ± 146.32 µm 3 (Hypoxia). Finally, cell volume on Laminin coating ended up to 581.52 ± 287.80 µm 3 for normoxic cells and 387.72 ± 155.73 µm 3 for hypoxic cultured cells. An overview of cell volumes values is shown in table 6 and figure 17. Tab. 6: Cell volume of normoxic and hypoxic cultured cells on different substrates. (N: Normoxia; H: Hypoxia). n = 25 cells. Cell volume [µm 3 ] Parameters Mean Median SD NUNC Collagen I Fibronectin Laminin N H N H N H N H 691.20 530.04 417.56 302.24 539.00 358.72 581.52 581.52 430.00 370.00 307.00 287.00 540.00 357.00 530.00 530.00 1,001.53 427.62 256.77 151.88 217.76 146.32 287.80 287.80

Results 35 Fig. 17: Cell volume measurement from white light confocal microscopy images. 25 cells per culture condition and substrate were evaluated. Normoxic as well as hypoxic cells showed some bigger outliers on polystyrene. Coating with collagen I, fibronectin or laminin decreased the volume distribution in both culture settings, whereat hypoxic cells appeared more homogenous. Median: dashed line; Mean: dotted line (dashed & dotted line: Median and Mean overlapping).

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