Views
4 years ago

Download

Download

Supplementary data 56 7.

Supplementary data 56 7. Supplementary data Supp.Tab. 1: Surface roughness of osteogenic differentiated hMSCs in Normoxia (N) & Hypoxia (H). Data was calculated with five fields of view. Mean & SD. parameters ost. differentiated control N H N H RMS [nm] 201.7 ± 35.1 176.0 ± 24.6 76.4 ± 5.6 105.5 ± 15.5 Ra [nm] 123.6 ± 22.9 104.1 ± 14.8 49.0 ± 3.3 74.6 ± 12.7 max. height [µm] 2.7 ± 0.5 2.9 ± 0.5 1.3 ± 0.5 2.4 ± 0.7 Supp.Tab. 2: Shape description overview of normoxic and hypoxic cultured hMSCs for each passage until senescence (n=100 cells). Mean & SD. Passage 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 Area [µm 2 ] Circularity Ferets diameter [µm] Aspect ratio N H N H N H N H 6,087.6 ± 4,539.3 ± 0.39 ± 0.29 ± 197.8 ± 183.1 ± 3.64 ± 3.78 ± 4,189.6 2,128.0 0.12 0.11 68.7 65.4 1.78 1.77 6,497.2 ± 5,191.3 ± 0.25 ± 0.24 ± 231.5 ± 195.1 ± 4.52 ± 3.54 ± 4,885.8 2,242.6 0.10 0.10 76.0 53.4 2.48 1.83 6,590.8 ± 4,231.2 ± 0.28 ± 0.29 ± 224.2 ± 171.4 ± 4.30 ± 3.50 ± 3,190.5 2,067.1 0.11 0.13 67.4 63.5 2.32 1.82 8,229.3 ± 5,592.9 ± 0.24 ± 0.28 ± 265.5 ± 212.8 ± 4.10 ± 4.10 ± 4,655.4 2,702.0 0.14 0.13 106.5 81.0 2.47 2.13 7,278.0 ± 5,043.1 ± 0.26 ± 0.34 ± 235.0 ± 182.9 ± 3.80 ± 3,59 ± 4,775.1 2,826.6 0.12 0.16 93.0 74.6 1.98 1.97 10,119.9 ± 5,545.3 ± 0.27 ± 0.29 ± 263.8 ± 200.2 ± 3.12 ± 3.87 ± 7,181.3 2,388.4 0.16 0.14 99.6 61.9 1.30 2.50 9,789.8 ± 4,643.1 ± 0.24 ± 0.34 ± 268.0 ± 176.8 ± 3.40 ± 3.86 ± 6,278.3 2,406.1 0.12 0.13 90.4 57.2 2.07 1.54 13,921.5 ± 4,968.8 ± 0.23 ± 0.29 ± 292.6 ± 187.6 ± 2.49 ± 3.62 ± 8,036.7 2,229.5 0.13 0.12 94.6 59.0 1.23 1.60 10,053.9 ± 5,196.3 ± 0.27 ± 0.30 ± 252.6 ± 184.8 ± 3.35 ± 3.33 ± 6,960.7 3,750.5 0.11 0.13 100.7 76.2 1.70 1.65 10,546.4 ± 4,820.4 ± 0.26 ± 0.30 ± 267.4 ± 190.4 ± 3.53 ± 3.91 ± 7,058.1 2,361.3 0.12 0.14 87.1 64.0 1.72 1.93 13,435.5 ± 6,047.9 ± 0.25 ± 0.33 ± 298.9 ± 191.2 ± 3.75 ± 3.08 ± 9,670.6 3,781.3 0.10 0.14 105.1 73.4 2.32 1.62 13,812.6 ± 6,812.8 ± 0.24 ± 0.32 ± 306.3 ± 200.7 ± 3.44 ± 3.40 ± 7,403.0 4,637.1 0.10 0.13 85.9 84.1 1.88 1.69 17,265.1 ± 10,418.5 ± 0.23 ± 0.32 ± 332.7 ± 251.4 ± 2.57 ± 2.74 ± 9,009.0 4,215.3 0.12 0.16 110.7 81.9 0.99 1.42 8,344.6 ± 0.34 ± 211.2 ± 2.71 ± 4,919.7 0.17 81.9 1.81 12,146.9 ± 0.39 ± 228.6 ± 2.37 ± 5,695.1 0.14 64.5 1.04 13,952.8 ± 0.28 ± 281.0 ± 2.51 ± 6,369.1 0.14 65.7 1.36 16,635.8 ± 0.39 ± 278.0 ± 2.43 ± 7,628.0 0.14 65.6 1.08 19,923.3 ± 0.37 ± 294.9 ± 2.21 ± 12,649.3 0.14 85.5 0.91

Supplementary data 57 Supp.Fig. 1: Tendency of shape description parameters for normoxic (red) and hypoxic (blue) cultured hMSCs. Cell area (A), circularity (B), Ferrets diameter (C) and aspect ratio (D) were calculated manually, in phase contrast images surrounded cells. 100 cells per passage and culture condition were included. Mean & SD. Supp.Tab. 3: Absolute cell migration velocities on different surfaces in normoxic and hypoxic cell culture conditions. abs. cell velocity [µm/min] parameters NUNC Collagen I Fibronectin Laminin N H N H N H N H Mean 0.27 0.44 0.39 0.56 0.41 0.46 0.40 0.57 SD 0.07 0.12 0.1 0.18 0.11 0.15 0.11 0.16

PDF: download this presentation (313 kb) - ecopa
Download article (PDF) - Miz.org
Download - UNESCO Deutschland
Download the Abstract Book as a PDF-file - German Cancer ...
Download Crestal Sinus Lift Kit Product Information - Osteogenics
Download - UNESCO Deutschland
Download programme brochure including Abstracts (as of 5 - NeBoCo
author's contributions - HTML