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Appendix 58 8. Appendix

Appendix 58 8. Appendix Tab. 7: Used laboratory equipment. autoclave, type Varioklav 300 H+P Labortechnik, Oberschleißheim, biophotometer Germany Eppendorf, Hamburg, Germany cell counter, Cedex XS Roche Innovatis, Bielefeld, Germany centrifuge, Universal 16 R Hettich, Tuttlingen, Germany confocal white light microscopy, PLµ 2300 Sensofar, Terrassa, Spain digital camera, IXUS 980 is Canon, Krefeld, Germany drying chamber, Jouan 42220400 Thermo Scientific, Schwerte, Germany ELISA reader, Multiskan FC Thermo Scientific, Schwerte, Germany Gel imager, Infinity-1000 Vilber Lourmat, Eberhardzell, Germany hypoxia cell incubator, MCO-5M Sanyo, München, Germany liquid nitrogen tank, Thermolyne Locator JR Barnstead, Dubuque, United States microscope camera, Axiocam ICc3 Carl Zeiss, Göttingen, Germany microscope camera, AxioCam MRm Carl Zeiss, Göttingen, Germany microscope camera, XC-HR58 Nikon, Düsseldorf, Germany microscope objective, 10x A-Plan Carl Zeiss, Göttingen, Germany microscope objective, LU Plan 50x/0,8 A Sensofar, Terrassa, Spain microscope, Axiovert S100 Carl Zeiss, Göttingen, Germany microscope, Axiovert, 40 CFL Carl Zeiss, Göttingen, Germany normoxia cell incubator, Hera cell 240 Thermo Scientific, Schwerte, Germany pH meter, pH 525 WTW, Weilheim, Germany pipette, Pipetman, 10, 20, 200 & 1000 µl Gilson, Middleton, United States pipetting aid, Pipettus Hirschmann, Eberstadt, Germany refrigerator, 561284 Liebherr, Ochsenhausen, Germany SCAN IM 130x100, automated micros- Märtzhäuser-Wetzlar GmbH & Co. KG, copy table Steindorf, Germany sterile cell culture flow Heraeus, Hanau, Germany thermocycler, DNA Engine® Bio-Rad, Munich, Germany

Appendix 59 vacuum pump for cell culture, VDE0530 Neuberger, Freiburg, Germany water bath, 1012 GFL, Burgwedel, Germany Tab. 8: Used consumables. aluminium foil, Universal 30 VWR, Darmstadt, Germany 35 mm cell culture dish (Nunclon TM ) Thermo Scientific, Schwerte, Germany balance bowl Roth, Karlsruhe, Germany cell counter slides, Cedex XS smart slide Roche Innovatis, Bielefeld, Germany cell culture flask (25, 75 & 225 cm 2 ) Nunc, Wiesbaden, Germany cell scraper (250 mm) Sarstedt, Nümbrecht, Germany cryogenic vials (1,8 ml) Nalgene, Rochester, United States cuvette, UVette Eppendorf, Hamburg, Germany disposable gloves, Peha-soft satin Hartmann, Heidenheim, Germany disposable pipette tibs (2, 5, 10 & 25 ml) Sarstedt, Nümbrecht, Germany disposable pipette tips (10, 20, 200, 1000 µl) Eppendorf, Hamburg, Germany Eppendorf reaction tube (1,5 ml) Eppendorf, Hamburg, Germany microscopy slides (SuperFrost ® ) Menzel-Gläser, Braunschweig, Germany Pasteur pipettes (230 mm) Brand, Wertheim, Germany Tab. 9: Used chemicals & cell culture media. 4% paraformaldehyd MicroCos GmbH, Garching, Germany adipogenic quantification kit, AdipoRed TM Lonza, Köln, Germany agarose (LE) Biozym, Hessisch Oldendorf, Germany AMV first strand cDNA synthesis kit Invitrogen, Karlsruhe, Germany CD105 (mouse anti-human FITC) BD Biosciences, Heidelberg, Germany CD14, CD19 & CD45 (mouse antihuman APC) Abcam, Cambridge, United Kingdom CD73 (mouse anti-human PE) BD Biosciences, Heidelberg, Germany CD90 (mouse anti-human PE-Cy5) BD Biosciences, Heidelberg, Germany cell culture media (D-MEM high glucose) PAA, Pasching, Austria cell culture medium (α-MEM) Invitrogen, Karlsruhe, Germany

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