5 years ago



Appendix 60 dexamethason

Appendix 60 dexamethason Sigma-Aldrich, Deisenhofen, Germany dimethyl sulfoxide (DMSO) Merck, Darmstadt, Germany Dulbecco’s phosphate buffered saline (PBS) PAA, Pasching, Austria ECM protein, human collagen type I BD Biosciences, Heidelberg, Germany ECM protein, human fibronectin BD Biosciences, Heidelberg, Germany ECM protein, mouse laminin BD Biosciences, Heidelberg, Germany ethanol (EtOH) Merck, Darmstadt, Germany fetal bovine serum (FBS) Sigma-Aldrich, Deisenhofen, Germany HLA-DR (mouse anti-human APC) Beckman Coulter, Krefeld, Germany indomethacin Sigma-Aldrich, Deisenhofen, Germany insulin Sigma-Aldrich, Deisenhofen, Germany isobutylmethylxanthine Sigma-Aldrich, Deisenhofen, Germany L-ascorbic acid Sigma-Aldrich, Deisenhofen, Germany mouse APC IgG1 isotype control Beckman Coulter, Krefeld, Germany mouse FITC IgG1 isotype control BD Biosciences, Heidelberg, Germany mouse PE IgG1 isotype control BD Biosciences, Heidelberg, Germany mouse PE-Cy5 IgG1 isoytpe control BD Biosciences, Heidelberg, Germany osteogenesis quantification kit Millipore, Billerica, USA Penicillin-Streptomycin-solution (100x) PAA, Pasching, Austria p-nitrophenyl N-acetyl-beta-Dglucosaminide (NPAG) Sigma-Aldrich, Deisenhofen, Germany poly-L-lysine, Cultrex ® R&D Systems, Wiesbaden, Germany RNeasy ® Mini Kit Qiagen, Hilden, Germany skim milk powder Merck, Darmstadt, Germany Trypsin-EDTA-solution (10x) PAA, Pasching, Austria Venor ® GeM Mycoplasma Detection Kit minerva-biolabs, Berlin, Germany WST-1 Assay Roche Diagnostics, Mannheim, Germany β-mercaptoethanol Merck, Darmstadt, Germany

Abbreviations 61 List of abbreviations AFM Atomic force microscope AR Alizarin Red ATP Adenosin-triphosphat BMP Bone morphogenetic protein CD Cluster of differentiation cDNA Complementary deoxyribonucleic acid Col1 Collagen type I CT Computer tomography Cum PD Cumulative population doublings ECM Extracellular matrix EDTA Ethylenediaminetetraacetic acid EEM Extraembryonic Membrane ExperiMed Experimental Surgery and Regenerative Medicine FACS Fluorescence activated cell sorting FBS Fetal bovine serum FC Flattened cell FN Fibronectin FOV Field of view FSS Flattened spindle-shape h Hour HIF Hypoxia inducible factor HIV Human immunodeficiency virus hMSC Human mesenchymal stem cell HSC Hematopoetic stem cell IBMX Isobutylmethylxanthine IGF Insulin-like growth factor IGFBP Insulin-like growth factor binding protein LMU Ludwig-Maximilian-University LN Laminin MEM Minimum essential media min Minute

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