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List of tables 66 List

List of tables 66 List of tables Tab. 1: Detailed description of CD markers. ......................................................................................... 13 Tab. 2: Morphological cell criteria of hMSCs......................................................................................... 14 Tab. 3: List of used primer sequences (F: forward, R: reverse, bp: base pairs)................................... 17 Tab. 4: Quantification of cell area and aspect ratio of 100 hMSCs per quarter in both culture conditions. Mean & SD............................................................................................................. 32 Tab. 5: Cell heights of normoxic and hypoxic cultured hMSCs on different surfaces........................... 32 Tab. 6: Cell volume of normoxic and hypoxic cultured cells on different substrates. (N: Normoxia; H: Hypoxia). n = 25 cells. .............................................................................................................. 34 Tab. 7: Used laboratory equipment....................................................................................................... 58 Tab. 8: Used consumables.................................................................................................................... 59 Tab. 9: Used chemicals & cell culture media. ....................................................................................... 59 Supp.Tab. 1: Surface roughness of osteogenic differentiated hMSCs in Normoxia (N) & Hypoxia (H). Data was calculated with five fields of view. Mean & SD. ........................................................ 56 Supp.Tab. 2: Shape description overview of normoxic and hypoxic cultured hMSCs for each passage until senescence (n=100 cells). Mean & SD. ........................................................................... 56 Supp.Tab. 3: Absolute cell migration velocities on different surfaces in normoxic and hypoxic cell culture conditions...................................................................................................................... 57

Acknowledgments 67 Acknowledgments Finally, I want appreciate following persons: Prof. Dr. med. Matthias Schieker, for providing me the topic and the access to his lab as well as helpful suggestions for the discussion. Furthermore, I want to thank him for an immense insight into the transferability from fundamental research to clinical relevance. Prof. Dr. rer. nat. Hauke Clausen-Schaumann, for providing me the access to his laboratory for Nanoanalytics and Biophysics. Additionally, I want thank him for his experienced scientific ideas, physical perception of biological systems and his abundant support during the discussion. Dr. med. Hans Polzer & Dr. rer. nat. Denitsa Docheva for their great suggestions and support over the last years. Dr. rer. biol. hum. Cvetan Popov for kindly providing me the integrin primers and for his helpful advices during the PCRs. M.Sc. Michael Kaiser for his friendly introduction into confocal white-light microscopy and competent knowledge during the cell volume discussion. M.Sc. Sebastian Schmidt, B.Sc. Sandra Bago & Robert Brunner, for their great input to biophysical topics. The whole ExperiMed Team, for all answered questions during this thesis. Special thanks to my parents for their long-lasting patience during my academic education.

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