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frequently used in the design of GMO antibiotic resistance genes, vector sequence,
selective markers, a sequence of "ori", auxotrophic sequence.
5.10.11.2. Confirmation of generic and specific accessories by polymerase chain
reaction (PCR) of 16S rRNA genes, and, if necessary – strain accessories by DNA-
DNA hybridization.
5.10.11.3. Identification of specific target genes of GMO is conducted: by PCR with
appropriate primers and subsequent sequencing, amplicon in the cases where the
nucleotide sequence of the target gene and its regulatory element is known; by
laboratory studies in accredited research center in accordance to the approved
methodological documents, in the cases the information about the nucleotide
composition of the target gene is missing.
5.10.11.4. Identification of the products of expression of the target gene, is conducted
by:
- Determining mRNA, transcribed from the target gene by reverse transcription -
polymerase chain reaction (RT-PCR);
- Identifying proteins expressed target gene TMG - by electrophoretic separation in
polyacrylamide gel (PAG - SDS);
- Determination of the specificity of the protein which is expressed with GMO target
gene – by using the immunoblot method.
5.10.11.5. A determination of the presence-absence of plasmids (with additional
control).
5.10.12. Hygienic assessment of foods derived from or using GMO or GMA pursued
with additional monitoring, including random checks of samples for compliance with the
requirements of this Sanitary rules according to the sanitary-chemical and
microbiological- sanitary quality and safety, or other research in accordance with Table
5 and 6.
5.10.13. Algorithms of laboratory samples of food products include 3 choices based on
information about the accessories used by microorganisms to GMA or GMO:
1) examination of samples of food products containing live microorganisms that are
genetically-modified analogues (GMA);
2) examination of samples of food products containing live genetically modified
microorganisms (GMOs);
3) study of samples of food products containing non-viable genetically modified microorganisms
and micro-organisms that are genetically-modified analogues, as well as
liberated from technological microflora.
5.10.14. Algorithm for laboratory examination of samples of food products containing
live GMA, provides as follows:
5.10.14.1. Analysis are conducted to samples of food and raw materials of the I and
the II groups (Table 1), obtained with or containing live GMA. The Procedure about
research can be consulted in Table 7.
Sample
subjects
Table 7
Algorithm for laboratory research for products
containing living GMA
Content Research Research Results decision