Boulant (PDF / 112.5 KB) - CellNetworks

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Boulant (PDF / 112.5 KB) - CellNetworks

BOULANT Steeve PhD

Laboratory of Viral Infection and Innate Immune Sensing

Dynamics

CHS Nachwuchsgruppe am CellNetworks Cluster und DKFZ.

Building 581 (TP4). Buro: 3.306

69120 Heidelberg

Phone: 06221 42 1560

Email: s.boulant@dkfz-heidelberg.de

PRESENT POSITION

CHS Nachwuchsgruppenleiter am CellNetworks Cluster und DKFZ,

Heidelberg University, Germany

RESEARCH EXPERIENCE

Oct 2004-

Feb 2005

2005-2006

2006-2008

2008-

present

Bridging grant fellow

Biochemical characterisation of the

hepatitis C virus core protein

Post-doctoral associate

Contribution of Lipid Droplets in

Hepatitis C virus life cycle

Marie curie post-doctoral fellow

Contribution of Lipid Droplets in

Hepatitis C virus life cycle

Post-doctoral associate

Study of non-enveloped virus entry

by clathrin-mediated endocytosis in

polarized cells

IBCP-CNRS

Lyon, France

Supervised by : Jean-Pierre Lavergne

(jp.lavergne@ibcp.fr) and François Penin

(f.penin@ibcp.fr)

MRC Virology Unit

Glasgow, Scotland, UK

Supervised by: John, McLauchlan

(j.mclauchlan@mrcvu.gla.ac.uk)

MRC Virology Unit

Glasgow, Scotland, UK

Supervised by: John, McLauchlan

(j.mclauchlan@mrcvu.gla.ac.uk)

Harvard Medical School

Boston, MA

Supervised by : Max Nibert

(max_nibert@hms.harvard.edu) and Tom

Kirchhausen

(kirchhausen@crystal.harvard.edu)

ACADEMIC TRAINING

Classe préparatoire Mathematic superior, Mathematic speciality. 1996-1998

Lycée Ozenne, Toulouse, France

Bachelor degree in Molecular Biology and Biochemistry. 1998-2000

Lyon-1 University

DEA in Molecular Biology and Biochemistry (Master). 2000-2001

Lyon-1 University

PhD in Molecular Biology and Biochemistry with excellence award. 2001-2004


Biochemical characterisation of the hepatitis C virus core protein, study of the lipid droplet

association domain, alternative forms of HCV core protein resulting from multiple recoding events.

Lyon-1 University, IBCP-CNRS, France. Supervised by: JP. LAVERGNE (jp.lavergne@ibcp.fr) and

F. PENIN (f.penin@ibcp.fr)

FELLOWSHIPS and FUNDINGs

Fellowships:

Post-doctoral fellowship granted by GlaxoSmithKline (2011-present)

Post-doctoral fellowship granted by Marie Curie Intra-European Fellowships (EIF) (2006-

2008)

Post-doctoral fellowship granted by the Medical Research Council (2005-2006)

Bridging grant granted by the “Fondation pour la Recherche Medicale” (October 2004-

February 2005)

Travelling Fellowship, International HCV conference, Heidelberg, Germany (2004)

EURODOC fellowship granted by the Rhône-Alpes region for a working visit to the

laboratory of Dr. J. McLauchlan, MRC Virology Unit, Glasgow, UK

PhD fellowship granted by the French ministry of research (2001-2004)

Funding:

Pilot and Feasibility award program HDDC, Boston, MA (2010)

Pilot and Feasibility award program HDDC, Boston, MA (2009)

AWARDS

Funding:

Pilot and Feasibility award program HDDC, Boston, MA (2010)

Pilot and Feasibility award program HDDC, Boston, MA (2009)

Awards:

Poster award, FASEB Lipid Droplets conference, Vermont, USA (2007)

Best Oral communication, UK Meeting on the Biology and Pathology of Hepatitis C Virus

(2006)

Travel award, 11 th International Meeting on HCV and Related Viruses (2005)

PUBLICATIONS

H index: 13. Average citations per items: 28. Total citations: 625

Accepted publication: 22

Submitted publications: 4

In preparation publications: 1

Corresponding author publication: 1 + 1submitted

First author publication: 10

Effect of temperature on clathrin mediated endocytosis

Steeve Boulant and Tom Kirchhausen in preparation

The first 10 seconds in the life of a clathrin coated pit

Ema Cocucci, Francois Aguet, Steeve Boulant and Tom Kirchhausen, Cell, Under Consideration


Real-Time Observation of Clathrin Dynamics in Three Dimensions

Steeve Boulant#, Comert Kural# , Tomas Kirchhausen Under Review

Cell-dependent differences in virus uptake correlate with receptor abundance

Steeve Boulant, Comert Kural, David Cureton, Ramiro Massol, Sean Whelan, Tomas Kirchhausen,

and Max L. Nibert submitted

Nucleolin is Required to Maintain the Architecture of Human Cytomegalovirus Replication

Compartments

Blair L Strang, Steeve Boulant, Tomas Kirchhausen and Donald M Coen. submitted.

Recruitment of cellular clathrin to viral factories by mammalian orthoreovirus protein μNS

Steeve Boulant#, Tijana Ivanovic#, Marcelo Ehrlich, Michelle M. Arnold, Tomas Kirchhausen, and

Max L. Nibert Traffic 2011

Actin provides additional force to counteract membrane tension during clathrin-mediated endocytosis

Steeve Boulant#, Comert Kural#, Jean-Christophe Zeeh and Tom Kirchhausen, Nat Cell Biol (IF

19.5) 2011

Perforin pores in the endosomal membrane trigger release of endocytosed granzyme B to the cytosol

of target cells

Jerome Thiery, Dennis Keefe, Steeve Boulant, Emmanuel Boucrot, Michael Walch, Denis

Martinvalet, Ing Swie Goping, R. Chris Bleackley, Tomas Kirchhausen, and Judy Lieberman Nature

immuno 2011

Peroxisomes and mitochondria cooperate to induce antiviral innate immunity

Evelyn Dixit, Steeve Boulant, Yijing Zhang, Amy S. Lee, Bennett O.V. Shum, Nir Hacohen , Zhijian

J. Chen, Sean P. Whelan, Marc Fransen, Max L. Nibert, Giulio Superti-Furga, Jonathan C. Kagan, Cell

(IF 32), 2010

Lipid Metabolism and HCV Infection

Paul Targett-Adams, Steeve Boulant , Mark W. Douglas and John McLauchlan, Review, Viruses (IF

2.8), 2010

Nucleolin associates with the human cytomegalovirus DNA polymerase accessory subunit UL44 and

is necessary for efficient viral replication.

Strang BL, Boulant S, Coen DM. J Virol (IF 5.3). 2010

Requirement of cellular DDX3 for hepatitis C virus replication is unrelated to its interaction with the

viral core protein.

Angus AG, Dalrymple D, Boulant S, McGivern DR, Clayton RF, Scott MJ, Adair R, Graham S,

Owsianka AM, Targett-Adams P, Li K, Wakita T, McLauchlan J, Lemon SM, Patel AH. J Gen Virol

(IF 3.2). 2010

Initiation of hepatitis C virus infection requires the dynamic microtubule network: role of the viral

nucleocapsid protein.

Roohvand F, Maillard P, Lavergne JP, Boulant S, Walic M, Andréo U, Goueslain L, Helle F, Mallet

A, McLauchlan J, Budkowska A J Biol Chem (IF 7.7). 2009

Subcellular localizations of the hepatitis C virus Alternate Reading Frame Proteins


Ratinier M, Boulant S, Crussard S, McLauchlan J and Lavergne JP. Virus Research (IF 2.8). 2008

Transcriptional slippage prompts recoding in alternate reading frames in the hepatitis C virus (HCV)

core sequence from strain HCV-1.

Ratinier M, Boulant S, Combet C, Targett-Adams P, McLauchlan J and Lavergne JP. J Gen Virol.

(IF 3.2) 2008.

Hepatitis C virus core protein induces lipid droplet redistribution in a microtubule- and dyneindependent

manner.

Boulant S, Douglas M, Moody L, Budkowska A, Targett-Adams P and McLauchlan J. Traffic. (IF

5.7) 2008.

Maturation of hepatitis C virus core protein by signal peptide peptidase is required for virus

production.

Targett-Adams P, Hope G, Boulant S and McLauchlan J. J Biol Chem (IF 7.7). 2008.

Visualization of double-stranded RNA in cells supporting hepatitis C virus RNA replication.

Targett-Adams P, Boulant S and McLauchlan J. J Virol.(IF 5.3) 2008.

Transcriptional slippage at a rare adenine cluster in the coding region of hepatitis C virus core protein

prompts recoding in alternate reading frames.

Ratinier M, Boulant S, Combet C, Targett-Adams P, McLauchlan J and Lavergne JP. J Gen Virol.

(IF 3.2) 2008.

The lipid droplet binding domain of hepatitis C virus core protein is a major determinant for efficient

virus assembly.

Shavinskaya A, Boulant S, Penin F, McLauchlan J and Bartenschlager R. J Biol Chem.(IF 7.7) 2007.

Disrupting the Association of Hepatitis C Virus Core Protein with Lipid Droplets Correlates with a

Loss in Production of Infectious Virus

Boulant S, Targett-Adams P and McLauchlan J. J Gen Virol. (IF 3.2) 2007.

Expression of the alternative reading frame protein of Hepatitis C virus induces cytokines involved in

hepatic injuries.

Fiorucci M, Boulant S, Fournillier A, Abraham JD, Lavergne JP, Paranhos-Baccala G, Inchauspe G

and Bain C. J Gen Virol. (IF 3.2) 2007.

Structural determinants that target the hepatitis C virus core protein to lipid droplets.

Boulant S, Montserret R, Hope RG, Ratinier M, Targett-Adams P, Lavergne JP, Penin F and

McLauchlan J. J Biol Chem. (IF 7.7) 2006.

Hepatitis C virus core protein is a dimeric alpha-helical protein exhibiting membrane protein features.

Boulant S, Vanbelle C, Ebel C, Penin F and Lavergne JP. J Virol.(IF 5.3) 2005.

Hepatitis C virus core protein acts as a trans-modulating factor on internal translation initiation of the

viral RNA.

Boni S, Lavergne JP, Boulant S and Cahour A. J Biol Chem. (IF 7.7) 2005.

The hepatitis C virus Core protein is a potent nucleic acid chaperone that directs dimerization of the

viral (+) strand RNA in vitro.


Cristofari G, Ivanyi-Nagy R, Gabus C, Boulant S, Lavergne JP, Penin F and Darlix JL. Nucleic Acids

Res.(IF 7.4) 2004.

Unusual multiple recoding events leading to alternative forms of hepatitis C virus core protein from

genotype 1b.

Boulant S, Becchi M, Penin F and Lavergne JP. J Biol Chem. (IF 7.7) 2003.

JOURNAL INVITED TO REVIEW FOR

Journal of General Virology

Virology

Science

PRESENTATIONS AT CONFERENCES

June 2011: Gordon conference virus and cells

"Cell-dependent differences in virus uptake correlate with receptor abundance"

October 2010: ASBMB membrane traffic symposium.

"Actin dynamics is essential to counteract membrane tension during clathrin-mediated endocytosis"

January 2010: EMBO membrane traffic workshop

"Study of clathrin mediated endocytosis in polarized cells"

September 2007: 13 th International Meeting on HCV and Related Viruses.

“HCV core protein induces lipid droplet redistribution: potential importance for the virus life cycle”

September 2005: 11 th International Meeting on HCV and Related Viruses.

“Structural determinants that target the hepatitis C virus core protein to lipid droplets”

October 2002: Annual meeting of HCV (French network).

“Evidence for an alternative hepatitis C virus core protein resulting from 2 translational

frameshiftings”

July 2002: 9 th International Meeting on HCV and Related Viruses.

“Evidence for an alternative hepatitis C virus core protein resulting from 2 translational

frameshiftings'

April 2002: 6 th day of EDISS (Ecole Doctorale Inter-Science et Santé)

“Characterizaion of alternative forms of the HCV core protein resulting from multiple recoding

events”

May 2001: 5 th day of EDISS (Ecole Doctorale Inter-Science et Santé).

“Biochemical characterisation of the hepatitis C virus core protein”

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