LMB Cambridge - Helsinki Institute of Physics
LMB Cambridge - Helsinki Institute of Physics
LMB Cambridge - Helsinki Institute of Physics
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Scientific Background to Electron Cryo-Microscopy<br />
Three Main types <strong>of</strong> Analysis (and the resolution attained<br />
in the analysed structures):<br />
1. Single Particle (molecule) Analysis 4 -10 Å<br />
2. Electron Crystallography <strong>of</strong> ordered specimen, i.e. 2-D<br />
crystals ~3Å … near-atomic resolution<br />
3. Electron Tomography 20-100 Å … cell biology<br />
Electron energy preferred: 300 keV (less lens aberrations,<br />
less multiple scattering in sample, less absorption)<br />
6/26/2008<br />
<strong>LMB</strong>, <strong>Cambridge</strong>
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