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1. INTRODUCTION - India Environment Portal

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microorganisms were procured from Hi-media (Pvt), Mumbai and their names are<br />

given below:<br />

<strong>1.</strong> Nutrient medium <strong>1.</strong> Total viable counts (TVC)<br />

2. Mac Conkey’s agar 2. Total Coliforms (TC)<br />

3. Escherichia coli like organisms (ECLO)<br />

All analyses were carried out within a few hours of collection. Water samples were<br />

directly used as inocula and sediment samples were serially diluted to give<br />

countable numbers expressed as number/ml of water or number/g of sediment.<br />

Surface plating technique was used and counting of plates were carried it after 48<br />

-72 hours of incubation at ambient temperature.<br />

<strong>1.</strong>3.4.2 Phytoplankton<br />

In the case of phytoplankton, samples were collected through net hauls (30µm<br />

mesh size) and 1 litre unit volume obtained at the surface. All net hauls were fixed<br />

in buffered 5% formaldehyde and stored until analysis in the laboratory. The unit<br />

samples (1 litre volume) on the other hand were treated with lugols iodine (10%)<br />

before storage. In the laboratory, these samples were allowed to stand for 36-48<br />

hrs in tall one litre measuring jars until all phytoplankton settled. Quantitative<br />

analysis was carried out on these samples and phytoplankton enumerated using a<br />

Sedgewick Rafter counting chamber after concentration with gravity sedimentation<br />

following a standard protocol (UNESCO, 1978). For taxonomic identification, a<br />

research microscope (Olympus, Japan, 400x) was put to use and the identification<br />

carried out according to Subrahmanyam (1946), DeBoyd and Smith (1977),<br />

Santhanam et al. (1987) and Tomas (1997).<br />

<strong>1.</strong>3.4.3 Zooplankton<br />

Zooplankton samples were collected by surface hauls with a Heron-Tranter net<br />

(mouth area 0.25m 2 , mesh size-300 µm) fitted with a calibrated flow meter. At<br />

20

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