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Imaging Capabilities at BRIL<br />
Carl Power<br />
Biological Resources Imaging Lab<br />
Mark Wainwright Analytical Centre<br />
<strong>University</strong> <strong>of</strong> New South Wales
Mark Wainwright Analytical Centre<br />
• Aim: to provide research support, training and collaboration via<br />
centrally-supported major experimental research facilities<br />
BRIL<br />
BMIF<br />
BMSF<br />
NMR<br />
SSEAU<br />
EMU<br />
Biological Resources Imaging Laboratory<br />
Fluorescence microscopy<br />
Mass spectrometry; proteomics<br />
NMR, EPR and vibrational spectroscopy<br />
X-Ray techniques (XPS, XRD, Crystallography)<br />
Elemental analysis (ICP, XRF, thermal analysis)<br />
Electron microscopy (SEM, TEM, FIB, SPM)
Challenges for small animal imaging<br />
• Resolution must be much higher than for clinical scanners<br />
• Adult mouse about 25 g : Adult human about 65kg<br />
• 0rgans in mice less than 1/1000 <strong>of</strong> human size<br />
• Dedicated scanners required. Clinical Scanners are rarely<br />
useful for imaging small research animals.<br />
• Animals must be anaesthetised<br />
• Animal must be supported! ‐ Hypothermia; dehydration<br />
• Attenuation <strong>of</strong> signal – tissue, bone, hair<br />
• Physiological movement – Respiration, Cardiac, Peristalsis<br />
• Gating required!
What is Available for Imaging?<br />
• Magnetic Resonance Imaging (MRI)<br />
• Positron Emission Tomography (PET)<br />
• SPECT (Single Photon Emission<br />
Computed Tomography)<br />
• microComputed Tomography<br />
(microCT)<br />
• Ultrasound<br />
• Optical Imaging System<br />
Bioluminescence Imaging<br />
Fluorescence Imaging<br />
• Intravital Fluorescence<br />
Microscopy<br />
• Photo‐accoustics<br />
• Endoscopy<br />
• X‐ray
Imaging modalities<br />
Imaging Modalities<br />
Anatomical<br />
Physiological Metabolic Molecular<br />
X‐Ray<br />
CT<br />
PET<br />
SPECT<br />
Ultrasound<br />
Optical<br />
MRI<br />
After Simon Cherry, UCDavis
Cancer imaging<br />
• Tumour growth and regression<br />
• Identification and enumeration <strong>of</strong> metastases<br />
• Vascularisation<br />
• Tumour necrosis and viability
Imaging <strong>of</strong> Cancer – Bioluminescence<br />
Identification <strong>of</strong> metastases<br />
lung<br />
left kidney<br />
spleen<br />
Day 7 Day 14 Day 17<br />
right kidney<br />
stomach<br />
Day 17<br />
(necropsy)<br />
cecum
Imaging <strong>of</strong> Cancer ‐ ultrasound<br />
Structural and Microvascular Imaging <strong>of</strong> tumours<br />
Courtesy <strong>of</strong> B Tse
Imaging <strong>of</strong> Cancer ‐ CT<br />
Imaging <strong>of</strong> osteopathic effects <strong>of</strong> tumours
Cardiovascular Imaging ‐ Ultrasound<br />
Vascular Imaging<br />
Foetal Cardiac Imaging<br />
Courtesy <strong>of</strong> M Kavurma<br />
Courtesy <strong>of</strong> H Ritchie
Vascular Imaging ‐ Ultrasound<br />
Cardiac Imaging<br />
Microvascular Imaging<br />
Courtesy <strong>of</strong> T‐T Hung<br />
Courtesy <strong>of</strong> B Tse and C Power
Neuro Imaging<br />
Assessment <strong>of</strong> Brain<br />
Tumour Growth ‐ BLI<br />
Placement <strong>of</strong> Cochlear<br />
Implants ‐ CT<br />
Courtesy <strong>of</strong> P Dilda<br />
Courtesy <strong>of</strong> J Pinyon and G Housley
Infectious Diseases<br />
S. Aureus infection in a knee Joint Colonisation <strong>of</strong> the Gut<br />
Images from Perkin Elmer
Infectious Diseases
Imaging inflammation<br />
Image from Perkin Elmer
Concluding remarks<br />
• Imaging technology is a powerful tool and<br />
becoming a standard for research with small<br />
animals<br />
• Numerous applications for all modalities<br />
• Multimodal imaging provides comprehensive<br />
and complimentary experimental readouts
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