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Surface-enhanced Raman scattering detection of Amyloid-β using a ...

Surface-enhanced Raman scattering detection of Amyloid-β using a ...

4.6

4.6 Raman spectroscopy .................................................................................................. 26 4.6.1 Set-up of the Raman experiment ........................................................................ 26 4.6.2 Raman measurements ........................................................................................ 27 5 Results ............................................................................................................................... 29 5.1 DEP fluorescence ....................................................................................................... 29 5.2 Nanoelectronic current .............................................................................................. 32 5.3 Raman spectroscopy .................................................................................................. 32 6 Discussion .......................................................................................................................... 34 6.1 Fluorescence observations ......................................................................................... 34 6.2 Nanoelectronic current measurements ...................................................................... 35 6.3 Raman spectra ........................................................................................................... 35 6.4 Comparison between the double-sided tape and the PDMS microfluidic channel ....... 36 6.5 Summary.................................................................................................................... 37 7 Outlook ............................................................................................................................. 39 Reference list ............................................................................................................................ 40 Appendix ................................................................................................................................... 43 Parameters and protocols .................................................................................................. 43 Acknowledgement..................................................................................................................... 44

List of abbreviations DEP DNA Aβ AD AC DC SERS F DEP CM PDMS CNC UV IPA DI ThT FAM CCD Dielectrophoresis Deoxyribonucleic acid Amyloid-β Alzheimer’s disease Alternating current Direct current Surface-enhanced Raman scattering Dielectrophoretic force Clausius-Mossotti Polydimethylsiloxane Computerized numerical control Ultraviolet Isopropyl alcohol deionized Thioflavin T Fluorescein amidite Charge-coupled device v

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