Roscoff Culture Collection - Station Biologique de Roscoff - Portail ...
Roscoff Culture Collection - Station Biologique de Roscoff - Portail ...
Roscoff Culture Collection - Station Biologique de Roscoff - Portail ...
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<strong>Roscoff</strong> <strong>Culture</strong> <strong>Collection</strong> CNRS et Université Pierre et Marie Curie<br />
mardi 3 juillet 2001<br />
RCC: 116<br />
Class<br />
Strain OTTH 0595 Sub Strain:<br />
Other Name<br />
Derived from:<br />
Prasinophyceae<br />
<strong>Culture</strong> isolation Oceanic origin Thau lagoon<br />
<strong>Station</strong>:<br />
CTD: Bottle: Depth:<br />
Temperature (°C):<br />
<strong>Culture</strong> conditions initally<br />
<strong>Culture</strong> conditions <strong>Roscoff</strong><br />
Medium SBR:<br />
K<br />
<strong>Culture</strong> purification<br />
axenisation<br />
Mixed<br />
Medium:<br />
Clonal<br />
Axenic<br />
Genus Ostreococcus<br />
Frozen<br />
Cruise:<br />
Toxic<br />
Species tauri<br />
Heterotrophic<br />
Date:<br />
Isolator<br />
Latitu<strong>de</strong>: ° ' Longitu<strong>de</strong> : ° '<br />
C. Courties<br />
Temperature <strong>Culture</strong> Origin 20 Date <strong>Culture</strong> arrived in SBR 10/12/97 Obtained From Courties<br />
Transfer period SBR (weeks) 2 Tube SBR: 17 Etiquettes: group B<br />
Temperature SBR (°C): 20 Light SBR (uE): 100<br />
Purification date: Purified by: Purification method:<br />
Date entered catalog: 01/11/98<br />
Publish to web catalog<br />
Lost<br />
Date Lost:<br />
Backup for: CCMP number:<br />
I<strong>de</strong>ntified by: Owned by:<br />
Light (uE):<br />
Method:<br />
Room SBR: 140<br />
<strong>Culture</strong> data<br />
Sent to Oslo<br />
Remarks<br />
I<strong>de</strong>ntification priority:<br />
Full study priority:<br />
Size 0.8<br />
Category<br />
Pigment analysis<br />
Pico<br />
DV 23/2/01: picture<br />
Email C. Courties du 7/3/2000<br />
Pour ce qui est d'O. tauri, bien sûr on peut le distribuer en s'assurant au préalable (par une analyse<br />
<strong>de</strong>s pigments ou mieux une image en microscopie électronique) que c'est bien O tauri que l'on<br />
donne. (j'ai par 2 fois envoyé une culture à Bidigare, sans succès sans doute, puisque je n'ai pas<br />
<strong>de</strong> nouvelles <strong>de</strong> mon 2° envoi fait il y a plusieurs mois).<br />
Pigment phenotype (for cyanos) :<br />
SSU rRNA and RFLP pattern<br />
Y15814<br />
Flow cytometry<br />
RCC<br />
116 Population number<br />
01/12/00 File RCC116<br />
Desc.<br />
RCC116 fluo nat<br />
Bead size 0.95<br />
Cell_ml 13513<br />
Setttings Values<br />
FSC E02 1.85<br />
SSC 450 0.68<br />
FL1<br />
FL2<br />
FL3 600<br />
0.00<br />
0.00<br />
13.94<br />
Remark<br />
<strong>Culture</strong>s are distributed for Aca<strong>de</strong>mic use only.<br />
Please contact D. Vaulot (vaulot@sb-roscoff.fr) for any information concerning this strain.
<strong>Roscoff</strong> <strong>Culture</strong> <strong>Collection</strong> CNRS et Université Pierre et Marie Curie<br />
mardi 3 juillet 2001<br />
RCC 141<br />
Class<br />
Strain EUM16BBL Sub Strain:<br />
Other Name<br />
Derived from:<br />
<strong>Culture</strong> isolation<br />
<strong>Station</strong>: OLIGO<br />
Temperature (°C):<br />
<strong>Culture</strong> conditions initally<br />
Remarks<br />
CTD:<br />
<strong>Culture</strong> conditions <strong>Roscoff</strong><br />
<strong>Culture</strong> purification<br />
axenisation<br />
<strong>Culture</strong> data<br />
Sent to Oslo<br />
Prasinophyceae<br />
Medium SBR:<br />
K<br />
Mixed<br />
Oceanic origin Tropical Atlantic Cruise: EUMELI 3<br />
Bottle:<br />
Clonal<br />
Size 1.5<br />
Depth: 105<br />
Date: 01/10/91<br />
Isolator<br />
Latitu<strong>de</strong>: 21 ° 2 ' Longitu<strong>de</strong> : 31 ° 8 '<br />
Temperature <strong>Culture</strong> Origin Date <strong>Culture</strong> arrived in SBR Obtained From<br />
Temperature SBR (°C): 20 Light SBR (uE): 4<br />
04/01. Sequence confirms it is very close to Ostreococcus oceanicus.<br />
Wenche 03/03. This is not Bathycoccus.<br />
DV jan 01. Very small coccoids almost black in PC. Ostreoccoccus? Make small clumps.<br />
09/2000, Florence: Coccoï<strong>de</strong>s <strong>de</strong>nses (1à2µm)<br />
Partensky<br />
Transfer period SBR (weeks) 2 Tube SBR: 21 Etiquettes: group A<br />
Purification date: Purified by: Purification method:<br />
I<strong>de</strong>ntification priority: 1<br />
Axenic<br />
Genus Ostreococcus<br />
Frozen<br />
Pigment analysis<br />
Toxic<br />
Species sp<br />
Heterotrophic<br />
Date entered catalog: 01/11/98<br />
Publish to web catalog<br />
Lost<br />
Date Lost:<br />
Backup for: CCMP number:<br />
I<strong>de</strong>ntified by: Owned by: SBR_Phyto<br />
Medium: mod. K/10<br />
Full study priority: 1<br />
Category<br />
Light (uE): 10<br />
Pico<br />
Method:<br />
Room SBR: 140<br />
Pigment phenotype (for cyanos) :<br />
W<br />
SSU rRNA and RFLP pattern<br />
ZZ006608 Prasinophyceae Ostreococcus oceanicus<br />
RC 141 Clone #<br />
Closest species<br />
Enzyme HaeIII Remarks<br />
556 377 295 260 177<br />
I<strong>de</strong>ntical to RCC<br />
RC Clone # Closest species Rhaphoneis belgicase (diatom)<br />
Mixed<br />
Flow cytometry<br />
RCC<br />
141 Population number 1<br />
01/12/00 File RCC141<br />
Desc.<br />
RCC141 fluo nat<br />
Bead size 0.95<br />
Cell_ml 12691<br />
Setttings Values<br />
FSC E02 1.06<br />
SSC 400 0.33<br />
FL1<br />
FL2<br />
FL3 600<br />
0.00<br />
0.00<br />
13.98<br />
Remark<br />
<strong>Culture</strong>s are distributed for Aca<strong>de</strong>mic use only.<br />
Please contact D. Vaulot (vaulot@sb-roscoff.fr) for any information concerning this strain.
<strong>Roscoff</strong> <strong>Culture</strong> <strong>Collection</strong> CNRS et Université Pierre et Marie Curie<br />
mardi 3 juillet 2001<br />
RCC 143<br />
Class<br />
Strain EUM13B Sub Strain:<br />
Other Name<br />
Derived from:<br />
<strong>Culture</strong> isolation<br />
<strong>Station</strong>: OLIGO<br />
Temperature (°C):<br />
<strong>Culture</strong> conditions initally<br />
Remarks<br />
CTD:<br />
<strong>Culture</strong> conditions <strong>Roscoff</strong><br />
<strong>Culture</strong> purification<br />
axenisation<br />
<strong>Culture</strong> data<br />
Sent to Oslo<br />
Prasinophyceae<br />
Medium SBR:<br />
1999: Pigments/Ultra by Laure<br />
EUM13BBL<br />
SSU rRNA and RFLP pattern<br />
ZZ003568<br />
K<br />
Mixed<br />
Oceanic origin Tropical Atlantic Cruise: EUMELI 3<br />
Bottle:<br />
Size 1.5<br />
Depth: 120<br />
Date: 01/10/91<br />
Isolator<br />
Latitu<strong>de</strong>: 21 ° 2 ' Longitu<strong>de</strong> : 31 ° 8 '<br />
Temperature <strong>Culture</strong> Origin Date <strong>Culture</strong> arrived in SBR Obtained From<br />
Temperature SBR (°C): 20 Light SBR (uE): 4<br />
Partensky<br />
Transfer period SBR (weeks) 4 Tube SBR: 19 Etiquettes: group H<br />
Purification date: Purified by: Purification method:<br />
I<strong>de</strong>ntification priority:<br />
Clonal<br />
Axenic<br />
Genus Ostreococcus<br />
Frozen<br />
Pigment analysis<br />
Toxic<br />
Species oceanicus<br />
Heterotrophic<br />
Date entered catalog: 01/11/98<br />
Publish to web catalog<br />
Lost<br />
Date Lost:<br />
Backup for: CCMP number:<br />
I<strong>de</strong>ntified by: L. Guillou Owned by: SBR_Phyto<br />
Medium: mod. K/10<br />
Full study priority: 1<br />
Category<br />
Light (uE): 10<br />
Pico<br />
Method:<br />
Room SBR: 140<br />
Pigment phenotype (for cyanos) :<br />
RCC 143 Pigment i<strong>de</strong>ntified by<br />
Filtration dat<br />
chl a<br />
chl b<br />
chl c1-2<br />
NP chl c2<br />
chl c3<br />
chl c-like<br />
Mg 3,8 DVP a5<br />
Mg 2,4 DVP a5<br />
chlorophylli<strong>de</strong> a<br />
9'-cis-néoxanthine<br />
alpha-carotène<br />
beta-carotène<br />
alpha+beta-carotène<br />
epsilon-carotène<br />
fucoxanthine<br />
19BF<br />
19HF<br />
lutéine<br />
W<br />
R. Barlow/N. Simo<br />
I<strong>de</strong>ntification date 01/01/94<br />
lutéine 5,8 epoxi<strong>de</strong><br />
prasinoxanthine<br />
prasinoxanthine-like<br />
siphonaxanthine-like<br />
siphonéine-like<br />
violaxanthine<br />
anthéraxanthine<br />
canthaxanthin<br />
vaucheriaxanthin<br />
zéaxanthine<br />
diadinoxanthine<br />
diatoxanthine<br />
Flow cytometry<br />
RCC<br />
143 Population number<br />
01/12/00 File RCC143<br />
Desc.<br />
RCC143 fluo nat<br />
Bead size 0.95<br />
Cell_ml 2231<br />
Setttings Values<br />
FSC<br />
3.30<br />
SSC<br />
0.53<br />
FL1<br />
0.00<br />
FL2<br />
0.00<br />
FL3<br />
26.46<br />
Remark<br />
<strong>Culture</strong>s are distributed for Aca<strong>de</strong>mic use only.<br />
Please contact D. Vaulot (vaulot@sb-roscoff.fr) for any information concerning this strain.
<strong>Roscoff</strong> <strong>Culture</strong> <strong>Collection</strong> CNRS et Université Pierre et Marie Curie<br />
mardi 3 juillet 2001<br />
RCC 393<br />
Class Prasinophyceae ?<br />
Strain PROSOPE_122-4 Sub Strain:<br />
Other Name<br />
Derived from:<br />
<strong>Culture</strong> isolation<br />
<strong>Station</strong>: 9<br />
Temperature (°C):<br />
<strong>Culture</strong> conditions initally<br />
<strong>Culture</strong> conditions <strong>Roscoff</strong><br />
Medium SBR:<br />
<strong>Culture</strong> purification<br />
axenisation<br />
<strong>Culture</strong> data<br />
Sent to Oslo<br />
Remarks<br />
12/6/01 DV Look like Ostreococuus<br />
Oceanic origin Mediterranean Sea Cruise: PROSOPE<br />
CTD: 70 Bottle: 9<br />
K<br />
Mixed<br />
Clonal<br />
Size 2<br />
Depth: 90<br />
Date: 28/09/99<br />
Isolator<br />
Latitu<strong>de</strong>: 10 ° 6 ' Longitu<strong>de</strong> : 41 ° 54 '<br />
Temperature <strong>Culture</strong> Origin Date <strong>Culture</strong> arrived in SBR Obtained From<br />
Temperature SBR (°C): 20 Light SBR (uE): 4<br />
Partensky<br />
Transfer period SBR (weeks) Tube SBR:<br />
Etiquettes: group O<br />
Purification date: 27/01/00 Purified by: F.Le Gall Purification method: Serial dilutions<br />
I<strong>de</strong>ntification priority: 1<br />
Full study priority:<br />
Axenic<br />
Genus Ostreococcus ?<br />
Frozen<br />
Pigment analysis<br />
Toxic<br />
Species<br />
Heterotrophic<br />
Date entered catalog: 22/03/01<br />
Publish to web catalog<br />
Lost<br />
Date Lost:<br />
Backup for: CCMP number:<br />
I<strong>de</strong>ntified by: Owned by: SBR_Phyto<br />
Medium: K/100<br />
Category<br />
Light (uE): 10<br />
Pico<br />
Method: 3µm<br />
Room SBR: 140<br />
Pigment phenotype (for cyanos) :<br />
E<br />
SSU rRNA and RFLP pattern<br />
Flow cytometry<br />
<strong>Culture</strong>s are distributed for Aca<strong>de</strong>mic use only.<br />
Please contact D. Vaulot (vaulot@sb-roscoff.fr) for any information concerning this strain.
<strong>Roscoff</strong> <strong>Culture</strong> <strong>Collection</strong> CNRS et Université Pierre et Marie Curie<br />
mardi 3 juillet 2001<br />
RCC 343<br />
Class<br />
Strain PROSOPE_5 Sub Strain:<br />
Other Name<br />
Derived from:<br />
<strong>Culture</strong> isolation<br />
<strong>Station</strong>: UPW<br />
Temperature (°C):<br />
<strong>Culture</strong> conditions initally<br />
<strong>Culture</strong> conditions <strong>Roscoff</strong><br />
<strong>Culture</strong> purification<br />
axenisation<br />
<strong>Culture</strong> data<br />
Sent to Oslo<br />
Remarks<br />
Prasinophyceae<br />
Medium SBR:<br />
Oceanic origin Atlantic Ocean/MaroccaCruise:<br />
CTD: 0 Bottle: 0<br />
Size 1<br />
Depth: 40<br />
PROSOPE<br />
Date: 12/09/99<br />
Isolator<br />
Latitu<strong>de</strong>: 30 ° 8 ' Longitu<strong>de</strong> : 10 ° 20 '<br />
Temperature <strong>Culture</strong> Origin Date <strong>Culture</strong> arrived in SBR Obtained From<br />
Temperature SBR (°C): 20 Light SBR (uE):<br />
DV 23/5/01. Miuscules cellules qui ressemblent à Ostreo. No contaminant<br />
Wenche 04/01: Wenche thinks it is Ostreococcus like<br />
Wenche Dec 00: Non-motile-few cells in culture. No scales or flagella observed in EM. Embed<strong>de</strong>d<br />
in Dec., but no pellet obtained.<br />
SSU rRNA and RFLP pattern<br />
RC 343 Clone #<br />
Enzyme<br />
HaeIII<br />
K<br />
Mixed<br />
Medium:<br />
Clonal<br />
Garczarek<br />
Transfer period SBR (weeks) Tube SBR:<br />
Etiquettes: group O<br />
Purification date: 04/02/00 Purified by: F.Le Gall Purification method: Cell Enrichment<br />
I<strong>de</strong>ntification priority: 1<br />
Full study priority: 1<br />
Remarks<br />
933 299 263 178<br />
Axenic<br />
Genus Ostreococcus<br />
Frozen<br />
Pigment analysis<br />
Toxic<br />
Species sp<br />
Heterotrophic<br />
Date entered catalog: 11/08/00<br />
Publish to web catalog<br />
Lost<br />
Date Lost:<br />
Backup for: CCMP number:<br />
I<strong>de</strong>ntified by: Owned by: SBR_Phyto<br />
Closest species<br />
Pro2/10<br />
Category<br />
Light (uE): 10<br />
Pico<br />
Nephroselmis olivacea ( Prasino<br />
I<strong>de</strong>ntical to RCC<br />
Mixed<br />
Method:<br />
0.6um<br />
Room SBR:<br />
Pigment phenotype (for cyanos) :<br />
RCC 343 Pigment i<strong>de</strong>ntified by<br />
Filtration dat<br />
chl a<br />
chl b<br />
chl c1-2<br />
NP chl c2<br />
chl c3<br />
chl c-like<br />
Mg 3,8 DVP a5<br />
Mg 2,4 DVP a5<br />
chlorophylli<strong>de</strong> a<br />
9'-cis-néoxanthine<br />
alpha-carotène<br />
beta-carotène<br />
alpha+beta-carotène<br />
epsilon-carotène<br />
fucoxanthine<br />
19BF<br />
19HF<br />
lutéine<br />
W<br />
F.Le Gall<br />
I<strong>de</strong>ntification date 13/06/00<br />
lutéine 5,8 epoxi<strong>de</strong><br />
prasinoxanthine<br />
prasinoxanthine-like<br />
siphonaxanthine-like<br />
siphonéine-like<br />
violaxanthine<br />
anthéraxanthine<br />
canthaxanthin<br />
vaucheriaxanthin<br />
zéaxanthine<br />
diadinoxanthine<br />
diatoxanthine<br />
Flow cytometry<br />
RCC<br />
343 Population number<br />
01/12/00 File RCC343<br />
Desc.<br />
RCC343 fluo nat<br />
Bead size 0.95<br />
Cell_ml 7582<br />
Setttings Values<br />
FSC E02 0.58<br />
SSC 450 0.34<br />
FL1<br />
FL2<br />
FL3 650<br />
0.00<br />
0.00<br />
5.48<br />
Remark<br />
<strong>Culture</strong>s are distributed for Aca<strong>de</strong>mic use only.<br />
Please contact D. Vaulot (vaulot@sb-roscoff.fr) for any information concerning this strain.
<strong>Roscoff</strong> <strong>Culture</strong> <strong>Collection</strong> CNRS et Université Pierre et Marie Curie<br />
mardi 3 juillet 2001<br />
RCC 344<br />
Class<br />
Strain PROSOPE_3 Sub Strain:<br />
Other Name<br />
Derived from:<br />
<strong>Culture</strong> isolation<br />
<strong>Station</strong>: UPW<br />
Temperature (°C):<br />
<strong>Culture</strong> conditions initally<br />
<strong>Culture</strong> conditions <strong>Roscoff</strong><br />
<strong>Culture</strong> purification<br />
axenisation<br />
<strong>Culture</strong> data<br />
Sent to Oslo<br />
Remarks<br />
Prasinophyceae<br />
Medium SBR:<br />
Oceanic origin Atlantic Ocean/MaroccaCruise:<br />
CTD: 0 Bottle: 0<br />
Size 1.5<br />
Depth: 5<br />
PROSOPE<br />
Date: 12/09/99<br />
Isolator<br />
Latitu<strong>de</strong>: 30 ° 8 ' Longitu<strong>de</strong> : 10 ° 20 '<br />
Temperature <strong>Culture</strong> Origin Date <strong>Culture</strong> arrived in SBR Obtained From<br />
Temperature SBR (°C): 20 Light SBR (uE):<br />
04/01: Sequences is very close to Ostreococcus. Very small cells (do not correspond to initial<br />
culture with pciture from Nov 00)....<br />
Wenche <strong>de</strong>c 00: Non-motile, few cells.<br />
SSU rRNA and RFLP pattern<br />
ZZ006585<br />
K<br />
RC 344 Clone # 4<br />
Enzyme<br />
HaeIII<br />
Mixed<br />
Medium:<br />
Clonal<br />
Garczarek<br />
Transfer period SBR (weeks) Tube SBR:<br />
Etiquettes: group O<br />
Purification date: 04/02/00 Purified by: F.Le Gall Purification method: Cell Enrichment<br />
I<strong>de</strong>ntification priority: 1<br />
Full study priority: 1<br />
1<br />
Remarks<br />
885 288 259 91<br />
Axenic<br />
Genus Ostreococcus<br />
Frozen<br />
Pigment analysis<br />
Toxic<br />
Species sp<br />
Heterotrophic<br />
Date entered catalog: 11/08/00<br />
Publish to web catalog<br />
Lost<br />
Date Lost:<br />
Backup for: CCMP number:<br />
I<strong>de</strong>ntified by: Owned by: SBR_Phyto<br />
Pro2/10<br />
Category<br />
Prasinophyceae Ostreococcus<br />
Closest species<br />
RC Clone # Closest species<br />
Light (uE): 100<br />
Pico<br />
I<strong>de</strong>ntical to RCC<br />
Mixed<br />
Method:<br />
0.6um<br />
Room SBR:<br />
Pigment phenotype (for cyanos) :<br />
RCC 344 Pigment i<strong>de</strong>ntified by<br />
Filtration dat<br />
chl a<br />
chl b<br />
chl c1-2<br />
NP chl c2<br />
chl c3<br />
chl c-like<br />
Mg 3,8 DVP a5<br />
Mg 2,4 DVP a5<br />
chlorophylli<strong>de</strong> a<br />
9'-cis-néoxanthine<br />
alpha-carotène<br />
beta-carotène<br />
alpha+beta-carotène<br />
epsilon-carotène<br />
fucoxanthine<br />
19BF<br />
19HF<br />
lutéine<br />
W<br />
F.Le Gall<br />
I<strong>de</strong>ntification date 13/06/00<br />
lutéine 5,8 epoxi<strong>de</strong><br />
prasinoxanthine<br />
prasinoxanthine-like<br />
siphonaxanthine-like<br />
siphonéine-like<br />
violaxanthine<br />
anthéraxanthine<br />
canthaxanthin<br />
vaucheriaxanthin<br />
zéaxanthine<br />
diadinoxanthine<br />
diatoxanthine<br />
Flow cytometry<br />
RCC<br />
344 Population number<br />
01/12/00 File RCC344<br />
Desc.<br />
RCC344 fluo nat<br />
Bead size 0.95<br />
Cell_ml 4908<br />
Setttings Values<br />
FSC E02 0.75<br />
SSC 450 0.35<br />
FL1<br />
FL2<br />
FL3 650<br />
0.00<br />
0.00<br />
5.38<br />
Remark<br />
<strong>Culture</strong>s are distributed for Aca<strong>de</strong>mic use only.<br />
Please contact D. Vaulot (vaulot@sb-roscoff.fr) for any information concerning this strain.
<strong>Roscoff</strong> <strong>Culture</strong> <strong>Collection</strong> CNRS et Université Pierre et Marie Curie<br />
mardi 3 juillet 2001<br />
RCC 356 Mixed Clonal Axenic Frozen Toxic Heterotrophic Date entered catalog: 06/11/00<br />
Class<br />
Publish to web catalog<br />
Prasinophyceae<br />
Genus Ostreococcus Species sp<br />
Strain RA000412-9 Other Name ASTAN_9<br />
Lost Date Lost:<br />
Sub Strain:<br />
Derived from:<br />
Backup for: CCMP number:<br />
I<strong>de</strong>ntified by: Sequence Owned by: SBR_Phyto<br />
<strong>Culture</strong> isolation<br />
<strong>Station</strong>: ASTAN<br />
Temperature (°C):<br />
<strong>Culture</strong> conditions initally<br />
Remarks<br />
CTD:<br />
<strong>Culture</strong> conditions <strong>Roscoff</strong><br />
Medium SBR:<br />
<strong>Culture</strong> purification<br />
axenisation<br />
<strong>Culture</strong> data<br />
Sent to Oslo<br />
Oceanic origin English Channel /Rosc<br />
Bottle:<br />
Size 1<br />
30/4/01: Sequences confirms as Ostreocococcus<br />
Wenche <strong>de</strong>c 00: Eupico, non-motile, olive-green.<br />
No scales present in EM. Embed<strong>de</strong>d <strong>de</strong>c. 00, very small pellet.<br />
SSU rRNA and RFLP pattern<br />
ZZ006646<br />
K<br />
RC 356 Clone # 8<br />
Enzyme<br />
HaeIII<br />
Medium: K/10<br />
Depth: 0<br />
Cruise:<br />
Date: 12/04/00<br />
Isolator<br />
Le Gall<br />
Latitu<strong>de</strong>: 48 ° 45 ' Longitu<strong>de</strong> : 3 ° 57 '<br />
Temperature <strong>Culture</strong> Origin 15 Date <strong>Culture</strong> arrived in SBR Obtained From<br />
Transfer period SBR (weeks) Tube SBR:<br />
Etiquettes: group O<br />
Temperature SBR (°C): 15 Light SBR (uE): 150<br />
Purification date: 11/10/00 Purified by: F.Le Gall Purification method: Dilution<br />
I<strong>de</strong>ntification priority: 1<br />
Full study priority: 1<br />
1<br />
Remarks<br />
486 422 288 253 90<br />
Category<br />
Light (uE): 150<br />
Pigment analysis<br />
Pico<br />
Chlorophyta Prasinophyceae Ostreococcus<br />
Closest species<br />
RC Clone # Closest species<br />
I<strong>de</strong>ntical to RCC<br />
Mixed<br />
Method: 3µm<br />
Room SBR: 158<br />
Pigment phenotype (for cyanos) :<br />
RCC 356 Pigment i<strong>de</strong>ntified by<br />
Filtration dat<br />
chl a<br />
chl b<br />
chl c1-2<br />
NP chl c2<br />
chl c3<br />
chl c-like<br />
Mg 3,8 DVP a5<br />
Mg 2,4 DVP a5<br />
chlorophylli<strong>de</strong> a<br />
9'-cis-néoxanthine<br />
alpha-carotène<br />
beta-carotène<br />
alpha+beta-carotène<br />
epsilon-carotène<br />
fucoxanthine<br />
19BF<br />
19HF<br />
lutéine<br />
W<br />
M. Latasa<br />
I<strong>de</strong>ntification date 01/04/01<br />
lutéine 5,8 epoxi<strong>de</strong><br />
prasinoxanthine<br />
prasinoxanthine-like<br />
siphonaxanthine-like<br />
siphonéine-like<br />
violaxanthine<br />
anthéraxanthine<br />
canthaxanthin<br />
vaucheriaxanthin<br />
zéaxanthine<br />
diadinoxanthine<br />
diatoxanthine<br />
Flow cytometry<br />
RCC<br />
356 Population number<br />
01/12/00 File ASTAN92<br />
Desc.<br />
QSTAN 9-2 fluo nat<br />
Bead size 0.95<br />
Cell_ml 16107<br />
Setttings Values<br />
FSC E01 0.69<br />
SSC 450 0.40<br />
FL1<br />
FL2<br />
FL3 600<br />
0.00<br />
0.00<br />
6.15<br />
Remark<br />
<strong>Culture</strong>s are distributed for Aca<strong>de</strong>mic use only.<br />
Please contact D. Vaulot (vaulot@sb-roscoff.fr) for any information concerning this strain.
<strong>Roscoff</strong> <strong>Culture</strong> <strong>Collection</strong> CNRS et Université Pierre et Marie Curie<br />
mardi 3 juillet 2001<br />
RCC 371<br />
Class<br />
Strain PROSOPE_44 Sub Strain:<br />
Other Name<br />
Derived from:<br />
<strong>Culture</strong> isolation<br />
<strong>Station</strong>: 5<br />
Temperature (°C):<br />
<strong>Culture</strong> conditions initally<br />
<strong>Culture</strong> conditions <strong>Roscoff</strong><br />
<strong>Culture</strong> purification<br />
axenisation<br />
<strong>Culture</strong> data<br />
Sent to Oslo<br />
Remarks<br />
Prasinophyceae<br />
Medium SBR:<br />
SSU rRNA and RFLP pattern<br />
Oceanic origin Mediterranean Sea Cruise: PROSOPE<br />
CTD: 23 Bottle: 10<br />
K<br />
Mixed<br />
Medium:<br />
Clonal<br />
Size 2<br />
Depth: 65<br />
Date: 18/09/99<br />
Isolator<br />
Latitu<strong>de</strong>: 13 ° 19 ' Longitu<strong>de</strong> : 36 ° 29 '<br />
Temperature <strong>Culture</strong> Origin Date <strong>Culture</strong> arrived in SBR Obtained From<br />
Temperature SBR (°C): 20 Light SBR (uE): 100<br />
Partensky<br />
Transfer period SBR (weeks) Tube SBR:<br />
Etiquettes: group O<br />
Purification date: 26/02/01 Purified by: F.Le Gall Purification method: Cell Enrichment<br />
I<strong>de</strong>ntification priority: 1<br />
Full study priority:<br />
Axenic<br />
Genus Ostreococcus ?<br />
Frozen<br />
Pigment analysis<br />
Toxic<br />
Species<br />
Heterotrophic<br />
Date entered catalog: 26/02/01<br />
Publish to web catalog<br />
Lost<br />
Date Lost:<br />
Backup for: CCMP number:<br />
I<strong>de</strong>ntified by: Owned by: SBR_Phyto<br />
Pro2/10+NO3<br />
Category<br />
Light (uE): 10<br />
Pico<br />
Method:<br />
0.6um<br />
Room SBR: 140<br />
Pigment phenotype (for cyanos) :<br />
RCC 371 Pigment i<strong>de</strong>ntified by<br />
Filtration dat<br />
chl a<br />
chl b<br />
chl c1-2<br />
NP chl c2<br />
chl c3<br />
chl c-like<br />
Mg 3,8 DVP a5<br />
Mg 2,4 DVP a5<br />
chlorophylli<strong>de</strong> a<br />
9'-cis-néoxanthine<br />
alpha-carotène<br />
beta-carotène<br />
alpha+beta-carotène<br />
epsilon-carotène<br />
fucoxanthine<br />
19BF<br />
19HF<br />
lutéine<br />
F.Le Gall<br />
I<strong>de</strong>ntification date 03/04/01<br />
lutéine 5,8 epoxi<strong>de</strong><br />
prasinoxanthine<br />
prasinoxanthine-like<br />
siphonaxanthine-like<br />
siphonéine-like<br />
violaxanthine<br />
anthéraxanthine<br />
canthaxanthin<br />
vaucheriaxanthin<br />
zéaxanthine<br />
diadinoxanthine<br />
diatoxanthine<br />
Flow cytometry<br />
<strong>Culture</strong>s are distributed for Aca<strong>de</strong>mic use only.<br />
Please contact D. Vaulot (vaulot@sb-roscoff.fr) for any information concerning this strain.
<strong>Roscoff</strong> <strong>Culture</strong> <strong>Collection</strong> CNRS et Université Pierre et Marie Curie<br />
mardi 3 juillet 2001<br />
RCC 410 Mixed Clonal Axenic Frozen Toxic Heterotrophic Date entered catalog: 18/06/01<br />
Class<br />
Publish to web catalog<br />
Prasinophyceae<br />
Genus Ostreococcus ? Species<br />
Strain REDSEA_20-3 Other Name EILAT_20-3<br />
Lost Date Lost:<br />
Sub Strain:<br />
Derived from:<br />
Backup for: CCMP number:<br />
I<strong>de</strong>ntified by: Owned by: SBR_Phyto<br />
<strong>Culture</strong> isolation<br />
<strong>Station</strong>: A<br />
Temperature (°C):<br />
<strong>Culture</strong> conditions initally<br />
Remarks<br />
CTD:<br />
<strong>Culture</strong> conditions <strong>Roscoff</strong><br />
Medium SBR:<br />
<strong>Culture</strong> purification<br />
axenisation<br />
<strong>Culture</strong> data<br />
Sent to Oslo<br />
K<br />
Oceanic origin Red Sea,Golfe d'Aqaba Cruise:<br />
Bottle:<br />
Size 1.5<br />
25/6/01: DV Look like Ostreo but less black.. Picture.<br />
Depth: 100<br />
Date: 13/09/00<br />
Isolator<br />
Latitu<strong>de</strong>: 29 ° 28 ' Longitu<strong>de</strong> : 34 ° 55 '<br />
Temperature <strong>Culture</strong> Origin Date <strong>Culture</strong> arrived in SBR Obtained From<br />
Temperature SBR (°C): 20 Light SBR (uE): 6<br />
Partensky<br />
Transfer period SBR (weeks) Tube SBR:<br />
Etiquettes: group O<br />
Purification date: 08/11/00 Purified by: F.Le Gall Purification method: Filtration+Cell Enrichment<br />
I<strong>de</strong>ntification priority:<br />
Full study priority:<br />
Medium: K/100<br />
Category<br />
Light (uE): 4<br />
Pigment analysis<br />
Pico<br />
Method:<br />
0.6µm<br />
Room SBR: 140<br />
Pigment phenotype (for cyanos) :<br />
E<br />
SSU rRNA and RFLP pattern<br />
Flow cytometry<br />
<strong>Culture</strong>s are distributed for Aca<strong>de</strong>mic use only.<br />
Please contact D. Vaulot (vaulot@sb-roscoff.fr) for any information concerning this strain.
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