LSM 5 EXCITER - Carl Zeiss

Fluorescence Mode
See Your Samples in a Different Light
The horizons of materials microscopy are broadening. Here is another angle
from which to analyze material properties and defects: In the fluorescence mode,
the LSM 5 EXCITER delivers unique information that increases your certainty in
assessing the structure and properties of your materials.
Complex materials analysis
Is a material impaired by structural impurities? Does it
change under mechanical loads? Are the constituents of
compositions distributed homogeneously, or are there
phase separations? Questions like these arise in the analysis
of polymers and fiber materials. The LSM 5 EXCITER working
in the fluorescence mode can answer them exactly. It
visualizes and measures autofluorescent inclusions, phases
or particle aggregations several hundreds of micrometers
deep in a semitransparent matrix.
Unlimited slope angles
The use of fluorescent dyes is helpful also in the visualization
and analysis of holes and crack patterns. Unlike in the
reflection mode, the image will show any slope angle up
to 90°.
Multifluorescence in biomaterials
Biomaterials play a growing part in basic research and
industry. Bioactive materials are being developed for medical
and dental use; neuro- and protein chips interface
information processing with biotechnologies. Where cell
aggregates and tissues in contact with inorganic materials
are investigated, multiple fluorescence experiments with
the LSM 5 EXCITER supply substantial information that is
difficult or impossible to extract by other microscopic techniques.
Front end of a polymeric optical fiber.
Fluorescence excitation at 633 nm.
3D shadow projections, rotated by 40° each.
Plan-Neofluar 10x/0.3;
1302.7 µm x 1302.7 µm x 1184.0 µm, 512 x 512 pixels x 161 sections.
Sample: Prof. Hartl, Prof. Dr. Poisel, Mr. Förster, Department EFI,
Georg-Simon-Ohm University of Applied Sciences, Nürnberg, Germany
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