2013 Promega catalogue

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Complete Solutions for Biobanking Workflows Promega solutions are designed to bring automation, efficiency and confidence to your biobanking workflow. • Automation: Instruments and chemistries designed to work together and provide higher throughput and greater reproducibility. • Flexibility: Scalable chemistries and modular instrument installation options adaptable to your biobanking throughput needs. • Confidence: Dependable products provided and supported by a single source helping you achieve an efficient, quality workflow Biobanking Extraction Quantitation Pre-Qualification Results 16 14 12 Brighter Fluorescence 10 dRn 8 6 4 2 0 Promega B Q IP IE R A Master Mix 8288MD Promega DNA purification chemistries and automation platforms for variable sample sizes and throughput needs. Fast, sensitive fluorescence and qPCR based Promega assays for accurate quantitation. Powerful STR systems for sample identification and contamination detection. Qualified, verified samples ready for storage or shipment. Start simplifying your workflow with solutions designed to work together: www.promega.com/Biobanking
Cell Signaling DNA Extraction for Biobanks ReliaPrep 96 gDNA Miniprep HT System Product Size Cat.# Price ($) ReliaPrep 96 gDNA Miniprep HT System 1 × 96 preps A2670 350 4 × 96 preps A2671 1260 Available Separately Size Conc. Cat.# Price ($) Heat Block Adapter 1 each A2661 582 RNase A Solution 5 ml 4 mg/ml A7974 Pls. Enq. 25mM Tris-HCl (pH 8.0) 60 ml A2641 30 10mM EDTA (pH 8.0) 10 ml A2631 30 20X TE Buffer (pH 7.5) 25 ml A2651 30 For Research Use Only. Not for Use in Diagnostic Procedures. Description: The ReliaPrep 96 gDNA Miniprep HT System provides a simple and reliable method for the rapid isolation of gDNA in a multiwell format. gDNA may be purified from blood and Oragene ® •Discover sample collection devices. The purified gDNA can be used directly in PCR assays, microarrays and nextgeneration sequencing applications. The use of paramagnetic particles for DNA capture eliminates the need for centrifugation or vacuum manifolds, making the system suitable for full automation. In addition, the system does not require an organic solvent, making it safe and convenient. DNA yields of up to 12µg are expected from input blood volumes of 350µl, depending on the WBC count of the sample. Saliva samples can have variable amounts of gDNA, and up to 18µg or more of DNA may be recovered from a 700µl Oragene ® collection device sample. Features: • Improve Productivity: Walkaway automation of genomic DNA extraction. • Eliminate Sample Rework: Robust, precipitation-free protocol, no chance of “lost pellets”. • Simplify Workflow: High yields of pure DNA from pristine and challenged or hemolysed samples. Protocol ReliaPrep 96 gDNA Miniprep HT System Protocol for the Beckman Coulter Biomek ® FX P ReliaPrep 96 gDNA Miniprep HT System Technical Manual Part# EP045 TM368 ReliaPrep Large Volume HT gDNA Isolation System Product Size Cat.# Price ($) ReliaPrep Large Volume HT 96 × 10ml to A1751 Pls. Enq. gDNA Isolation System 960 × 1ml preps Available Separately Size Cat.# Price ($) ReliaPrep LV 32 HSM Instrument 1 each A1715 Pls. Enq. ReliaPrep LV 32 HSM Standard Service Agreement 1 each SA3070 Pls. Enq. ReliaPrep Cover 1 each A1712 Pls. Enq. ReliaPrep Tube Rack 1 each A1713 Pls. Enq. ReliaPrep Tube Rack Stand 1 each A1714 Pls. Enq. MagnaBot ® Flat Top Magnetic Separation Device 1 each V6041 Pls. Enq. A1751 For Laboratory Use. A1715, SA3070, A1712, A1713, A1714, V6041 For Research Use Only. Not for Use in Diagnostic Procedures. Description: The ReliaPrep Large Volume HT gDNA Isolation System isolates genomic DNA (gDNA) from 3–10ml of blood in a scalable format. The chemistry eliminates tedious centrifugation steps as well as the use of hazardous chemicals, which are inherent in precipitation-based chemistries. The system has been automated on robotic liquid-handling workstations, allowing walkaway purification of genomic DNA from 3–10ml of whole blood, regardless of sample storage or shipping conditions. For low-throughput isolation of gDNA from up to 32 samples at one time, the ReliaPrep LV 32 HSM can be used in a manual mode, where the user performs the pipetting functions. The HSM has an LCD screen that directs the user through the protocol. Features: • Decrease Hands-On Time: Automation reduces operator time spent on instrument setup and takedown by allowing walkaway operation for large numbers of samples at a time. • Remove Protocol Bottlenecks: Heater Shaker Magnet eliminates the need to move samples on the robot deck, reducing instrument failures; precipitation-free chemistry dramatically reduces purification failures. • Achieve Peace of Mind: Automated liquid level sensing for all samples and solutions with operator notification allows recovery of samples in case of error. • Isolate Pure DNA from All Samples: Purification chemistry is equally effective at recovering DNA from pristine as well as challenged (hemolysed or frozen) samples. • Save a Day or Two of Processing: Samples are eluted in buffer, ready for use in downstream assays or archiving, eliminating resuspension of pelleted DNA, which can take 24–48 hours. • Reduce Waste: Chemistry is automatically scaled for each sample, using only the reagent required for optimal purification. Plastic use is also conserved, reducing liquid and solid waste during sample runs. Storage Conditions: Store at 15–30°C. 1 Biobanking Protocol ReliaPrep LV 32 HSM Instrument Technical Manual ReliaPrep Large Volume HT gDNA Isolation System Technical Manual Part# TM326 TM341 Section Contents For complete and up-to-date product information visit: www.promega.com/catalog 3 Table of Contents
Page 1 and 2: Life Science CATALOG 2013 Table of
Page 3 and 4: Cell Signaling Table of Contents Pr
Page 5: Cell Signaling 1 Biobanking 1 DNA E
Page 9 and 10: Cell Signaling Wizard ® Genomic DN
Page 11 and 12: Cell Signaling QuantiFluor RNA Syst
Page 13 and 14: Cell Signaling Sample ID and Mixed
Page 15 and 16: Cell Signaling 2 Biochemicals and L
Page 17 and 18: Cell Signaling Agarose, LMP, Prepar
Page 19 and 20: Cell Signaling Blue/Orange Loading
Page 21 and 22: Cell Signaling Glycine, Molecular B
Page 23 and 24: Cell Signaling SDS Solution, Molecu
Page 25 and 26: Cell Signaling Tris Base, Molecular
Page 27 and 28: Cell Signaling Unmethylated Lambda
Page 29 and 30: Cell Signaling Promega Barrier Tips
Page 31 and 32: Cell Signaling Magnetic Stands and
Page 33 and 34: Cell Signaling 3 Cell Health Assays
Page 35 and 36: Cell Signaling A. B. Luminescence/p
Page 37 and 38: Cell Signaling Luminogenic Enzyme S
Page 39 and 40: Cell Signaling Apoptosis
Page 41 and 42: Cell Signaling Caspase-Glo ® 2 Ass
Page 43 and 44: Cell Signaling Caspase-Glo ® 8 Ass
Page 45 and 46: Cell Signaling CaspACE Assay System
Page 47 and 48: Cell Signaling Anti-ACTIVE ® Caspa
Page 49 and 50: Cell Signaling CellTiter-Glo ® One
Page 51 and 52: Cell Signaling Fluorescent Cell Via
Page 53 and 54: Cell Signaling CellTiter-Blue ® Ce
Page 55 and 56: Cell Signaling ApoTox-Glo Triplex A
Page 57 and 58:
Cell Signaling CytoTox-Fluor Cytoto
Page 59 and 60:
Cell Signaling GSH-Glo Glutathione
Page 61 and 62:
Cell Signaling 4 Cell Signaling 4 G
Page 63 and 64:
Cell Signaling cAMP-Glo Max Assay P
Page 65 and 66:
Cell Signaling Growth Factors Epide
Page 67 and 68:
Cell Signaling SIRT-Glo Assays and
Page 69 and 70:
Cell Signaling ADP-Glo Kinase Assay
Page 71 and 72:
Cell Signaling Kinase Enzyme System
Page 73 and 74:
Page 75 and 76:
Page 77 and 78:
Page 79 and 80:
Page 81 and 82:
Cell Signaling ProFluor ® PKA Assa
Page 83 and 84:
Cell Signaling PepTag ® Non-Radioa
Page 85 and 86:
Cell Signaling Product Size Cat.# P
Page 87 and 88:
Cell Signaling kDa 98 - 64 - 50 - 3
Page 89 and 90:
Cell Signaling ProFluor ® Ser/Thr
Page 91 and 92:
Cell Signaling 5 Cloning and DNA Ma
Page 93 and 94:
Cell Signaling DNA Step Ladders Pro
Page 95 and 96:
Cell Signaling Conventional DNA Mar
Page 97 and 98:
Cell Signaling RNA Markers Product
Page 99 and 100:
Cell Signaling AatII 37 Product Siz
Page 101 and 102:
Cell Signaling BstEII 60 Product Si
Page 103 and 104:
Cell Signaling HhaI 37 Product Size
Page 105 and 106:
Cell Signaling NdeI 37 Product Size
Page 107 and 108:
Cell Signaling SnaBI 37 Product Siz
Page 109 and 110:
Cell Signaling Alkaline Phosphatase
Page 111 and 112:
Cell Signaling T4 DNA Polymerase Pr
Page 113 and 114:
Cell Signaling Kinases and DNA Labe
Page 115 and 116:
Cell Signaling Additional Enzymes S
Page 117 and 118:
Cell Signaling Subcloning Tools and
Page 119 and 120:
Cell Signaling pALTER ® -MAX Vecto
Page 121 and 122:
Cell Signaling pGEM ® -7Zf(+/-) Ve
Page 123 and 124:
Cell Signaling pSP72 Vector Product
Page 125 and 126:
Cell Signaling 6 DNA and RNA Purifi
Page 127 and 128:
Cell Signaling x-tracta Gel Extract
Page 129 and 130:
Cell Signaling Wizard ® MagneSil
Page 131 and 132:
Cell Signaling ReliaPrep Blood gDNA
Page 133 and 134:
Cell Signaling Wizard ® SV 96 Geno
Page 135 and 136:
Cell Signaling Fixed-Tissue Genomic
Page 137 and 138:
Cell Signaling Maxwell ® 16 System
Page 139 and 140:
Cell Signaling PureYield Plasmid Mi
Page 141 and 142:
Cell Signaling Wizard ® Plus Minip
Page 143 and 144:
Cell Signaling Wizard ® SV 96 and
Page 145 and 146:
Cell Signaling RNA Purification Rel
Page 147 and 148:
Cell Signaling PureYield RNA Midipr
Page 149 and 150:
Page 151 and 152:
Cell Signaling RNasin ® Plus RNase
Page 153 and 154:
Cell Signaling DNA and RNA Quantita
Page 155 and 156:
Cell Signaling Magnetic Stands and
Page 157 and 158:
Cell Signaling Vacuum Manifolds and
Page 159 and 160:
Cell Signaling 7 Drug Discovery 7 D
Page 161 and 162:
Cell Signaling GPCR Assays cAMP-Glo
Page 163 and 164:
Cell Signaling GloResponse Lucifera
Page 165 and 166:
Cell Signaling DPPIV-Glo Protease A
Page 167 and 168:
Cell Signaling Calpain-Glo Protease
Page 169 and 170:
Cell Signaling 8 Epigenetics 8 DNA
Page 171 and 172:
Cell Signaling DNA Purification Tec
Page 173 and 174:
Cell Signaling Luciferase-Based Met
Page 175 and 176:
Cell Signaling GoTaq ® Long PCR Ma
Page 177 and 178:
Cell Signaling DUB-Glo Protease Ass
Page 179 and 180:
Cell Signaling HaloTag ® Protein P
Page 181 and 182:
Cell Signaling 9 Genetic Identity 9
Page 183 and 184:
Cell Signaling DNA IQ System Produc
Page 185 and 186:
Cell Signaling DNA IQ Casework Pro
Page 187 and 188:
Cell Signaling STR Analysis for For
Page 189 and 190:
Cell Signaling PowerPlex ® Y23 Sys
Page 191 and 192:
Cell Signaling PowerPlex ® 18D Sys
Page 193 and 194:
Cell Signaling PowerPlex ® S5 Syst
Page 195 and 196:
Cell Signaling GenePrint ® Fluores
Page 197 and 198:
Cell Signaling 10 Imaging and Immun
Page 199 and 200:
Cell Signaling HaloTag ® Ligand Bu
Page 201 and 202:
Cell Signaling NGF E max ® ImmunoA
Page 203 and 204:
Cell Signaling Anti-ACTIVE ® CaM K
Page 205 and 206:
Cell Signaling A. Anti-ACTIVE ® MA
Page 207 and 208:
Cell Signaling Anti-ACTIVE ® Caspa
Page 209 and 210:
Cell Signaling Anti-NGF mAb Product
Page 211 and 212:
Cell Signaling Anti-TGFβ 1 pAb Pro
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Cell Signaling Horseradish Peroxida
Page 215 and 216:
Cell Signaling ViviRen In Vivo Reni
Page 217 and 218:
Cell Signaling 11 Industrial and En
Page 219 and 220:
Cell Signaling QuantiLum ® Recombi
Page 221 and 222:
12 Instruments 12 Luminometers 218
Page 223 and 224:
GloMax ® 20/20 Luminometer Product
Page 225 and 226:
Fluorescence Module: Application-op
Page 227 and 228:
GloMax ® -Multi Jr Single-Tube Mul
Page 229 and 230:
Maxwell ® CSC System for IVD Use M
Page 231 and 232:
Maxwell ® Research Systems Maxwell
Page 233 and 234:
Maxwell ® 16 Flexi Method Firmware
Page 235 and 236:
Cell Signaling 13 Molecular Diagnos
Page 237 and 238:
Cell Signaling Maxwell ® CSC Servi
Page 239 and 240:
Page 241 and 242:
Cell Signaling Maxwell ® 16 Flexi
Page 243 and 244:
Cell Signaling Deoxynucleotide Trip
Page 245 and 246:
Cell Signaling 14 PCR 14 Hot-Start
Page 247 and 248:
Cell Signaling Routine PCR GoTaq ®
Page 249 and 250:
Cell Signaling Pfu DNA Polymerase d
Page 251 and 252:
Cell Signaling GoTaq ® Real-Time q
Page 253 and 254:
Cell Signaling StemElite Human Panc
Page 255 and 256:
Cell Signaling Reverse Transcriptio
Page 257 and 258:
Cell Signaling AMV Reverse Transcri
Page 259 and 260:
Cell Signaling PCR Cloning pGEM ®
Page 261 and 262:
Cell Signaling 15 Protein Expressio
Page 263 and 264:
Cell Signaling TnT ® Quick Coupled
Page 265 and 266:
Cell Signaling TnT ® T7 Quick for
Page 267 and 268:
Cell Signaling Canine Pancreatic Mi
Page 269 and 270:
Cell Signaling E. coli S30 Extract
Page 271 and 272:
Cell Signaling Mass Spectrometry An
Page 273 and 274:
Cell Signaling Endoproteinase Lys-C
Page 275 and 276:
Cell Signaling Protein Labeling and
Page 277 and 278:
Cell Signaling HaloTag ® Fusion (C
Page 279 and 280:
Cell Signaling Inducible T7-Driven
Page 281 and 282:
Cell Signaling ECL Western Blotting
Page 283 and 284:
Cell Signaling 16 Protein Purificat
Page 285 and 286:
Cell Signaling HaloTag ® Protein P
Page 287 and 288:
HT Cell Signaling HaloTag ® Mammal
Page 289 and 290:
Cell Signaling HaloLink Protein Arr
Page 291 and 292:
Cell Signaling FastBreak Cell Lysis
Page 293 and 294:
Cell Signaling MagneHis Protein Pur
Page 295 and 296:
Cell Signaling Streptavidin Product
Page 297 and 298:
Cell Signaling Protein Purification
Page 299 and 300:
Cell Signaling 17 Reporter Assays a
Page 301 and 302:
Cell Signaling NanoLuc Luciferase
Page 303 and 304:
Cell Signaling Chroma-Glo Luciferas
Page 305 and 306:
Cell Signaling ONE-Glo + Tox Lucife
Page 307 and 308:
Cell Signaling Luciferase Assay Sys
Page 309 and 310:
Cell Signaling ViviRen Live Cell Su
Page 311 and 312:
Cell Signaling Reporter Vectors and
Page 313 and 314:
Cell Signaling Promoterless Renilla
Page 315 and 316:
Cell Signaling pmirGLO Dual-Lucifer
Page 317 and 318:
Cell Signaling pGL2 Luciferase Repo
Page 319 and 320:
Cell Signaling Reporter Vector Sequ
Page 321 and 322:
Cell Signaling pCAT ® 3 Vectors Pr
Page 323 and 324:
Cell Signaling ViviRen In Vivo Reni
Page 325 and 326:
Cell Signaling 18 RNA Analysis 18 I
Page 327 and 328:
Cell Signaling Riboprobe ® System
Page 329 and 330:
Cell Signaling HeLaScribe ® Nuclea
Page 331 and 332:
Cell Signaling RNA Interference Gen
Page 333 and 334:
Cell Signaling 19 Stem Cell Researc
Page 335 and 336:
Cell Signaling Cell ID System Fluor
Page 337 and 338:
Cell Signaling StemElite Human Panc
Page 339 and 340:
Cell Signaling 20 STR Analysis 20 C
Page 341 and 342:
Cell Signaling PowerPlex ® 16 HS S
Page 343 and 344:
Cell Signaling StemElite ID System
Page 345 and 346:
Cell Signaling 21 Vectors 21 Bacter
Page 347 and 348:
Cell Signaling Mammalian Expression
Page 349 and 350:
Cell Signaling pCI-neo Mammalian Ex
Page 351 and 352:
Page 353 and 354:
Cell Signaling pSV-β-Galactosidase
Page 355 and 356:
Page 357 and 358:
22 Index 22 Index: A-Z 354 Index by
Page 359 and 360:
Index: A-Z Click on name to go to p
Page 361 and 362:
Page 363 and 364:
Page 365 and 366:
Index by Catalog Number Click on pa
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Page 387 and 388:
1S11 TH01 D3S1358 FGA TPOX D8S1179
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2013 Promega catalogue

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