RNA Guide: Purifying RNA and mRNA - Promega
RNA Guide: Purifying RNA and mRNA - Promega
RNA Guide: Purifying RNA and mRNA - Promega
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<strong>Purifying</strong> <strong>RNA</strong> <strong>and</strong> m<strong>RNA</strong><br />
Automated<br />
Blood<br />
Manual<br />
<strong>RNA</strong> yield<br />
from blood<br />
varies with<br />
white cell count<br />
MagneSil ® Total <strong>RNA</strong><br />
mini-Isolation System<br />
PureYield <strong>RNA</strong><br />
Midiprep System<br />
SV Total <strong>RNA</strong><br />
Isolation System<br />
Unique product!<br />
Novel clearing agent<br />
removes all detectable<br />
genomic DNA.<br />
No DNase<br />
treatment needed!<br />
<strong>RNA</strong>gents ® Total<br />
<strong>RNA</strong> Isolation System<br />
Scalable solution-based<br />
reagent that can<br />
process up to 1gm of<br />
tissue in a single prep<br />
5587MA<br />
DNase included to<br />
greatly reduce<br />
gDNA carry-over.<br />
Can process 20µl<br />
whole blood in<br />
96-well format;<br />
5µl whole blood in<br />
384-well format<br />
Protocols require<br />
removal of red blood<br />
cells with SV Red<br />
Cell Lysis Solution<br />
prior to purification<br />
In addition to <strong>RNA</strong> isolation from mammalian<br />
blood, the SV Total <strong>RNA</strong> Isolation System has<br />
been used on related non-mammalian sources,<br />
including marine invertebrate hemolymph <strong>and</strong><br />
fish red blood cells.<br />
10<br />
<strong>Promega</strong> <strong>RNA</strong> Analysis Notebook
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