3 years ago

by Henrik E Poulsen - CPNC

by Henrik E Poulsen - CPNC

Methodologies: Urine

Methodologies: Urine cells HPLC-ECD HPLC-MS X X ? X HPLC-MS/MS X X ELISA (X) (X) COMET ASSAY Alkaline Elution X X

HPLC system: Perkin Elmer 200 series Mass spectrometer: API 3000 triple quadrupole, PE Biosystems HPLC Ionisation inlet to high Vac Q1 Q2 Q3 Mass Det. Collision gass Turbo Ion-spray + 5 kV 0 kV 35000 30000 25000 20000 15000 10000 LC-MS/MS analysis 11 modified and 8 unmodified products FaPy Ade FaPy Gua "8oxoGua" DHdU 5OHdC DHdT 5OHMedU 2oxodA 8oxoGuo 8oxodG 8oxodA FaPy Ade 5OHMedU "8oxoGua" 5000 8oxo RiboThymidin Guo DHdU 8oxodG FaPy Gua Gua 0 -5 0 5 10 15 20 25 30 35 dC U RiboThymidin Ado dC dG Gua U 5OHdC DHdT dT Guo 2oxodA dT Guo dG dA 8oxodA Ado

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