HPTLC for the analysis of botanicals - Sistem e-Warga - Universiti ...

Speakers’ Profile
Dr. Eike Reich is Head of CAMAG Laboratory. He studied
chemistry and earned his PhD from Humboldt University
Berlin for the isolation and identification of Crinum
alkaloids. Dr. Reich was Associate Professor of
Chemistry at Longwood College, Virginia, where his
research fields included chromatography of pheromones
and plant constituents. Currently he is specialized in the
application of HPTLC for the analysis of medicinal plants.
Dr. Reich is member of the European Pharmacopoeia
and the USP Expert committee on Dietary Supplements
(General Chapters). He works closely with the American
Herbal Pharmacopoeia.
Below are some of his publications:
• Validation of High-Performance Thin-Layer Chromatographic
Methods for the Identification of
Botanicals in a cGMP Environment. Reich, E., Schibli,
A., DeBatt, A. (2008) J. AOAC Int. 91, 13-20. Appendix
(complete documentation and method write-up).
• Modern TLC: A Key Technique for Identification and
Quality Control of Botanicals and Dietary Supplements.
Schibli, A., Reich, E. (2005) J. Planar Chromatogr.
18, 34-38
• A Standardized Approach to Modern High Performance
Thin-Layer Chromatography (HPTLC). Reich,
E., Blatter, A., (2002), in Proceedings of the International
Symposium "Planar Chromatography Today"
(Vovk, I., and Medja, A., Eds), 7-21, Novo Mesto.
Location Map
Location
DK4, Level 2, Block J,
Faculty of Pharmacy,
Universiti Kebangsaan Malaysia Kampus KL,
Jalan Raja Muda Abdul Aziz,
50300 Kuala Lumpur.
For more information, please contact:
1. Ms Rowyna Kueh
Phone: 012 213 2004
Email: rowyna.kueh@dksh.com
or
2. Ms Naini Zain
Phone: 03 7954 8888 ext 316
Email: naini.zain@dksh.com
HPTLC for the analysis of
botanicals
Venue: DK4, Level 2, Block J,
Faculty of Pharmacy,
UKM KL Campus
Date: June 3-4, 2009
Time: 8:30am - 5:00pm
For complete listing of his publications, please visit
http://www.camag.com/laboratory/publications/ownpublications.html
Co-organized by
Drug Delivery and Discovery Research Group, UKM-TKP-22-104/2008
Faculty of Pharmacy, Universiti Kebangsaan Malaysia, KL Campus
Think Asia. Think DKSH.

Introduction
Workshop agenda
Instrumental Thin-Layer Chromatography (or Planar
Chromatography) is a modern separation technique,
established worldwide and distinguished by flexibility,
reliability and cost efficiency. Together with HPLC and GC
it belongs to the microanalytical methods, which play an
important role in research and routine laboratories. In
many cases instrumental Thin-Layer Chromatography
offers a more suitable solution and often it is used as
confirmatory or alternative technique.
Performing thin-layer chromatographic separation on
HPTLC layers has several advantages over those on
conventional layers:
•
•
•
•
Higher resolution of zones due to higher number of
theoretical plates
Shorter developing times
Less solvent consumption
Less background noise due to narrow size distribution
of particles
However, suitable instruments are required to get the best
results.
Objective
This workshop aims to provide basic concepts on HPTLC
as well as further understanding on the method
development and troubleshooting.
Registration Fee
Student
Non-student
RM50 per person
RM100 per person
Day 1 - June 3, 2009 (Wednesday)
Basic concepts
8:30am - 9:15am
9:15am - 9:30am
9:30am - 10:45am
10:45am - 11:00am
11:00am - 1:00pm
1:00pm - 2:00pm
2:00pm - 3:30pm
3:30pm - 3:45pm
3:45pm - 5:00pm
Registration
Welcome speech
Lecture 1: Modern TLC and HPTLC
- Parameters of the invidual TLC
steps
- The state of the art in HPTLC
- Standardized methodology and
SOP for HPTLC
- HPTLC in a cGMP environment
Tea break
Lecture 2: Utilization of TLC-
Fingerprints for ID of botanicals
- TLC in the pharmacopoeias
- Botanical ID vs. chemical profile
- Requirements for “HPTLC fingerprints”
- Working with compendial methods
Lunch
Demonstration 1: Practical
aspects of modern TLC/HPTLC
- The tools
- HPTLC methodology
- The software
- Creating methods
Tea break
Demonstration 2: Identification of
botanicals by HPTLC
- Running an analysis
- Performing image comparison
Day 2 - June 4, 2009 (Thursday)
Method development and troubleshooting
8:30am - 9:00am
9:00am - 10:45am
10:45am - 11:00am
11:00am - 1:00pm
1:00pm - 2:00pm
2:00pm - 3:30pm
3:30pm - 3:45pm
3:45pm - 5:00pm
Registration
Lecture 3 : Optimizing the separation
- Separation in HPTLC
- Stationary and mobile phase
- General optimizing strategies
Tea break
Demonstration 3: Quantitative
determinations
- Scanning densitometry
- Video densitometry
Lunch
Demonstration 4: Method development
and validation
- SOP for method evaluation /
development?
- Validation protocol - qualitative
methods
Tea break
Lecture 4: Development and
validation of a method
- Setting the goal
- Sample preparation
- The CAMAG-Optimizaiton Scheme
- Validation concepts