HPTLC for the analysis of botanicals - Sistem e-Warga - Universiti ...
HPTLC for the analysis of botanicals - Sistem e-Warga - Universiti ...
HPTLC for the analysis of botanicals - Sistem e-Warga - Universiti ...
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Speakers’ Pr<strong>of</strong>ile<br />
Dr. Eike Reich is Head <strong>of</strong> CAMAG Laboratory. He studied<br />
chemistry and earned his PhD from Humboldt University<br />
Berlin <strong>for</strong> <strong>the</strong> isolation and identification <strong>of</strong> Crinum<br />
alkaloids. Dr. Reich was Associate Pr<strong>of</strong>essor <strong>of</strong><br />
Chemistry at Longwood College, Virginia, where his<br />
research fields included chromatography <strong>of</strong> pheromones<br />
and plant constituents. Currently he is specialized in <strong>the</strong><br />
application <strong>of</strong> <strong>HPTLC</strong> <strong>for</strong> <strong>the</strong> <strong>analysis</strong> <strong>of</strong> medicinal plants.<br />
Dr. Reich is member <strong>of</strong> <strong>the</strong> European Pharmacopoeia<br />
and <strong>the</strong> USP Expert committee on Dietary Supplements<br />
(General Chapters). He works closely with <strong>the</strong> American<br />
Herbal Pharmacopoeia.<br />
Below are some <strong>of</strong> his publications:<br />
• Validation <strong>of</strong> High-Per<strong>for</strong>mance Thin-Layer Chromatographic<br />
Methods <strong>for</strong> <strong>the</strong> Identification <strong>of</strong><br />
Botanicals in a cGMP Environment. Reich, E., Schibli,<br />
A., DeBatt, A. (2008) J. AOAC Int. 91, 13-20. Appendix<br />
(complete documentation and method write-up).<br />
• Modern TLC: A Key Technique <strong>for</strong> Identification and<br />
Quality Control <strong>of</strong> Botanicals and Dietary Supplements.<br />
Schibli, A., Reich, E. (2005) J. Planar Chromatogr.<br />
18, 34-38<br />
• A Standardized Approach to Modern High Per<strong>for</strong>mance<br />
Thin-Layer Chromatography (<strong>HPTLC</strong>). Reich,<br />
E., Blatter, A., (2002), in Proceedings <strong>of</strong> <strong>the</strong> International<br />
Symposium "Planar Chromatography Today"<br />
(Vovk, I., and Medja, A., Eds), 7-21, Novo Mesto.<br />
Location Map<br />
Location<br />
DK4, Level 2, Block J,<br />
Faculty <strong>of</strong> Pharmacy,<br />
<strong>Universiti</strong> Kebangsaan Malaysia Kampus KL,<br />
Jalan Raja Muda Abdul Aziz,<br />
50300 Kuala Lumpur.<br />
For more in<strong>for</strong>mation, please contact:<br />
1. Ms Rowyna Kueh<br />
Phone: 012 213 2004<br />
Email: rowyna.kueh@dksh.com<br />
or<br />
2. Ms Naini Zain<br />
Phone: 03 7954 8888 ext 316<br />
Email: naini.zain@dksh.com<br />
<strong>HPTLC</strong> <strong>for</strong> <strong>the</strong> <strong>analysis</strong> <strong>of</strong><br />
<strong>botanicals</strong><br />
Venue: DK4, Level 2, Block J,<br />
Faculty <strong>of</strong> Pharmacy,<br />
UKM KL Campus<br />
Date: June 3-4, 2009<br />
Time: 8:30am - 5:00pm<br />
For complete listing <strong>of</strong> his publications, please visit<br />
http://www.camag.com/laboratory/publications/ownpublications.html<br />
Co-organized by<br />
Drug Delivery and Discovery Research Group, UKM-TKP-22-104/2008<br />
Faculty <strong>of</strong> Pharmacy, <strong>Universiti</strong> Kebangsaan Malaysia, KL Campus<br />
Think Asia. Think DKSH.
Introduction<br />
Workshop agenda<br />
Instrumental Thin-Layer Chromatography (or Planar<br />
Chromatography) is a modern separation technique,<br />
established worldwide and distinguished by flexibility,<br />
reliability and cost efficiency. Toge<strong>the</strong>r with HPLC and GC<br />
it belongs to <strong>the</strong> microanalytical methods, which play an<br />
important role in research and routine laboratories. In<br />
many cases instrumental Thin-Layer Chromatography<br />
<strong>of</strong>fers a more suitable solution and <strong>of</strong>ten it is used as<br />
confirmatory or alternative technique.<br />
Per<strong>for</strong>ming thin-layer chromatographic separation on<br />
<strong>HPTLC</strong> layers has several advantages over those on<br />
conventional layers:<br />
•<br />
•<br />
•<br />
•<br />
Higher resolution <strong>of</strong> zones due to higher number <strong>of</strong><br />
<strong>the</strong>oretical plates<br />
Shorter developing times<br />
Less solvent consumption<br />
Less background noise due to narrow size distribution<br />
<strong>of</strong> particles<br />
However, suitable instruments are required to get <strong>the</strong> best<br />
results.<br />
Objective<br />
This workshop aims to provide basic concepts on <strong>HPTLC</strong><br />
as well as fur<strong>the</strong>r understanding on <strong>the</strong> method<br />
development and troubleshooting.<br />
Registration Fee<br />
Student<br />
Non-student<br />
RM50 per person<br />
RM100 per person<br />
Day 1 - June 3, 2009 (Wednesday)<br />
Basic concepts<br />
8:30am - 9:15am<br />
9:15am - 9:30am<br />
9:30am - 10:45am<br />
10:45am - 11:00am<br />
11:00am - 1:00pm<br />
1:00pm - 2:00pm<br />
2:00pm - 3:30pm<br />
3:30pm - 3:45pm<br />
3:45pm - 5:00pm<br />
Registration<br />
Welcome speech<br />
Lecture 1: Modern TLC and <strong>HPTLC</strong><br />
- Parameters <strong>of</strong> <strong>the</strong> invidual TLC<br />
steps<br />
- The state <strong>of</strong> <strong>the</strong> art in <strong>HPTLC</strong><br />
- Standardized methodology and<br />
SOP <strong>for</strong> <strong>HPTLC</strong><br />
- <strong>HPTLC</strong> in a cGMP environment<br />
Tea break<br />
Lecture 2: Utilization <strong>of</strong> TLC-<br />
Fingerprints <strong>for</strong> ID <strong>of</strong> <strong>botanicals</strong><br />
- TLC in <strong>the</strong> pharmacopoeias<br />
- Botanical ID vs. chemical pr<strong>of</strong>ile<br />
- Requirements <strong>for</strong> “<strong>HPTLC</strong> fingerprints”<br />
- Working with compendial methods<br />
Lunch<br />
Demonstration 1: Practical<br />
aspects <strong>of</strong> modern TLC/<strong>HPTLC</strong><br />
- The tools<br />
- <strong>HPTLC</strong> methodology<br />
- The s<strong>of</strong>tware<br />
- Creating methods<br />
Tea break<br />
Demonstration 2: Identification <strong>of</strong><br />
<strong>botanicals</strong> by <strong>HPTLC</strong><br />
- Running an <strong>analysis</strong><br />
- Per<strong>for</strong>ming image comparison<br />
Day 2 - June 4, 2009 (Thursday)<br />
Method development and troubleshooting<br />
8:30am - 9:00am<br />
9:00am - 10:45am<br />
10:45am - 11:00am<br />
11:00am - 1:00pm<br />
1:00pm - 2:00pm<br />
2:00pm - 3:30pm<br />
3:30pm - 3:45pm<br />
3:45pm - 5:00pm<br />
Registration<br />
Lecture 3 : Optimizing <strong>the</strong> separation<br />
- Separation in <strong>HPTLC</strong><br />
- Stationary and mobile phase<br />
- General optimizing strategies<br />
Tea break<br />
Demonstration 3: Quantitative<br />
determinations<br />
- Scanning densitometry<br />
- Video densitometry<br />
Lunch<br />
Demonstration 4: Method development<br />
and validation<br />
- SOP <strong>for</strong> method evaluation /<br />
development?<br />
- Validation protocol - qualitative<br />
methods<br />
Tea break<br />
Lecture 4: Development and<br />
validation <strong>of</strong> a method<br />
- Setting <strong>the</strong> goal<br />
- Sample preparation<br />
- The CAMAG-Optimizaiton Scheme<br />
- Validation concepts