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HPTLC for the analysis of botanicals - Sistem e-Warga - Universiti ...

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Speakers’ Pr<strong>of</strong>ile<br />

Dr. Eike Reich is Head <strong>of</strong> CAMAG Laboratory. He studied<br />

chemistry and earned his PhD from Humboldt University<br />

Berlin <strong>for</strong> <strong>the</strong> isolation and identification <strong>of</strong> Crinum<br />

alkaloids. Dr. Reich was Associate Pr<strong>of</strong>essor <strong>of</strong><br />

Chemistry at Longwood College, Virginia, where his<br />

research fields included chromatography <strong>of</strong> pheromones<br />

and plant constituents. Currently he is specialized in <strong>the</strong><br />

application <strong>of</strong> <strong>HPTLC</strong> <strong>for</strong> <strong>the</strong> <strong>analysis</strong> <strong>of</strong> medicinal plants.<br />

Dr. Reich is member <strong>of</strong> <strong>the</strong> European Pharmacopoeia<br />

and <strong>the</strong> USP Expert committee on Dietary Supplements<br />

(General Chapters). He works closely with <strong>the</strong> American<br />

Herbal Pharmacopoeia.<br />

Below are some <strong>of</strong> his publications:<br />

• Validation <strong>of</strong> High-Per<strong>for</strong>mance Thin-Layer Chromatographic<br />

Methods <strong>for</strong> <strong>the</strong> Identification <strong>of</strong><br />

Botanicals in a cGMP Environment. Reich, E., Schibli,<br />

A., DeBatt, A. (2008) J. AOAC Int. 91, 13-20. Appendix<br />

(complete documentation and method write-up).<br />

• Modern TLC: A Key Technique <strong>for</strong> Identification and<br />

Quality Control <strong>of</strong> Botanicals and Dietary Supplements.<br />

Schibli, A., Reich, E. (2005) J. Planar Chromatogr.<br />

18, 34-38<br />

• A Standardized Approach to Modern High Per<strong>for</strong>mance<br />

Thin-Layer Chromatography (<strong>HPTLC</strong>). Reich,<br />

E., Blatter, A., (2002), in Proceedings <strong>of</strong> <strong>the</strong> International<br />

Symposium "Planar Chromatography Today"<br />

(Vovk, I., and Medja, A., Eds), 7-21, Novo Mesto.<br />

Location Map<br />

Location<br />

DK4, Level 2, Block J,<br />

Faculty <strong>of</strong> Pharmacy,<br />

<strong>Universiti</strong> Kebangsaan Malaysia Kampus KL,<br />

Jalan Raja Muda Abdul Aziz,<br />

50300 Kuala Lumpur.<br />

For more in<strong>for</strong>mation, please contact:<br />

1. Ms Rowyna Kueh<br />

Phone: 012 213 2004<br />

Email: rowyna.kueh@dksh.com<br />

or<br />

2. Ms Naini Zain<br />

Phone: 03 7954 8888 ext 316<br />

Email: naini.zain@dksh.com<br />

<strong>HPTLC</strong> <strong>for</strong> <strong>the</strong> <strong>analysis</strong> <strong>of</strong><br />

<strong>botanicals</strong><br />

Venue: DK4, Level 2, Block J,<br />

Faculty <strong>of</strong> Pharmacy,<br />

UKM KL Campus<br />

Date: June 3-4, 2009<br />

Time: 8:30am - 5:00pm<br />

For complete listing <strong>of</strong> his publications, please visit<br />

http://www.camag.com/laboratory/publications/ownpublications.html<br />

Co-organized by<br />

Drug Delivery and Discovery Research Group, UKM-TKP-22-104/2008<br />

Faculty <strong>of</strong> Pharmacy, <strong>Universiti</strong> Kebangsaan Malaysia, KL Campus<br />

Think Asia. Think DKSH.


Introduction<br />

Workshop agenda<br />

Instrumental Thin-Layer Chromatography (or Planar<br />

Chromatography) is a modern separation technique,<br />

established worldwide and distinguished by flexibility,<br />

reliability and cost efficiency. Toge<strong>the</strong>r with HPLC and GC<br />

it belongs to <strong>the</strong> microanalytical methods, which play an<br />

important role in research and routine laboratories. In<br />

many cases instrumental Thin-Layer Chromatography<br />

<strong>of</strong>fers a more suitable solution and <strong>of</strong>ten it is used as<br />

confirmatory or alternative technique.<br />

Per<strong>for</strong>ming thin-layer chromatographic separation on<br />

<strong>HPTLC</strong> layers has several advantages over those on<br />

conventional layers:<br />

•<br />

•<br />

•<br />

•<br />

Higher resolution <strong>of</strong> zones due to higher number <strong>of</strong><br />

<strong>the</strong>oretical plates<br />

Shorter developing times<br />

Less solvent consumption<br />

Less background noise due to narrow size distribution<br />

<strong>of</strong> particles<br />

However, suitable instruments are required to get <strong>the</strong> best<br />

results.<br />

Objective<br />

This workshop aims to provide basic concepts on <strong>HPTLC</strong><br />

as well as fur<strong>the</strong>r understanding on <strong>the</strong> method<br />

development and troubleshooting.<br />

Registration Fee<br />

Student<br />

Non-student<br />

RM50 per person<br />

RM100 per person<br />

Day 1 - June 3, 2009 (Wednesday)<br />

Basic concepts<br />

8:30am - 9:15am<br />

9:15am - 9:30am<br />

9:30am - 10:45am<br />

10:45am - 11:00am<br />

11:00am - 1:00pm<br />

1:00pm - 2:00pm<br />

2:00pm - 3:30pm<br />

3:30pm - 3:45pm<br />

3:45pm - 5:00pm<br />

Registration<br />

Welcome speech<br />

Lecture 1: Modern TLC and <strong>HPTLC</strong><br />

- Parameters <strong>of</strong> <strong>the</strong> invidual TLC<br />

steps<br />

- The state <strong>of</strong> <strong>the</strong> art in <strong>HPTLC</strong><br />

- Standardized methodology and<br />

SOP <strong>for</strong> <strong>HPTLC</strong><br />

- <strong>HPTLC</strong> in a cGMP environment<br />

Tea break<br />

Lecture 2: Utilization <strong>of</strong> TLC-<br />

Fingerprints <strong>for</strong> ID <strong>of</strong> <strong>botanicals</strong><br />

- TLC in <strong>the</strong> pharmacopoeias<br />

- Botanical ID vs. chemical pr<strong>of</strong>ile<br />

- Requirements <strong>for</strong> “<strong>HPTLC</strong> fingerprints”<br />

- Working with compendial methods<br />

Lunch<br />

Demonstration 1: Practical<br />

aspects <strong>of</strong> modern TLC/<strong>HPTLC</strong><br />

- The tools<br />

- <strong>HPTLC</strong> methodology<br />

- The s<strong>of</strong>tware<br />

- Creating methods<br />

Tea break<br />

Demonstration 2: Identification <strong>of</strong><br />

<strong>botanicals</strong> by <strong>HPTLC</strong><br />

- Running an <strong>analysis</strong><br />

- Per<strong>for</strong>ming image comparison<br />

Day 2 - June 4, 2009 (Thursday)<br />

Method development and troubleshooting<br />

8:30am - 9:00am<br />

9:00am - 10:45am<br />

10:45am - 11:00am<br />

11:00am - 1:00pm<br />

1:00pm - 2:00pm<br />

2:00pm - 3:30pm<br />

3:30pm - 3:45pm<br />

3:45pm - 5:00pm<br />

Registration<br />

Lecture 3 : Optimizing <strong>the</strong> separation<br />

- Separation in <strong>HPTLC</strong><br />

- Stationary and mobile phase<br />

- General optimizing strategies<br />

Tea break<br />

Demonstration 3: Quantitative<br />

determinations<br />

- Scanning densitometry<br />

- Video densitometry<br />

Lunch<br />

Demonstration 4: Method development<br />

and validation<br />

- SOP <strong>for</strong> method evaluation /<br />

development?<br />

- Validation protocol - qualitative<br />

methods<br />

Tea break<br />

Lecture 4: Development and<br />

validation <strong>of</strong> a method<br />

- Setting <strong>the</strong> goal<br />

- Sample preparation<br />

- The CAMAG-Optimizaiton Scheme<br />

- Validation concepts

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