Annual 2005 - Hauptman Woodward Institute - University at Buffalo

President’s Message
The Human Frontier Science Program (HFSP) Organization, headquartered in Strasbourg,
France, supports basic research in the life sciences with emphasis placed on novel, innovative,
and interdisciplinary approaches that involve international (preferably intercontinental)
collaborations. I am pleased to report that the grant application “New Methods of Biomolecular
Crystal Structure Determination Specific to Neutron Diffraction Data,” for which I am
the Principal Applicant, recently was awarded $350,000 per year for a period of three
years by the HFSP Board of Trustees. The HFSP grants are extremely competitive. This year,
approximately 800 grants were submitted, but only 20 were funded. We are honored by the
fact that, of these 20, ours was ranked first. Furthermore, this is the first time that an HWI
scientist has received funding from an international organization.
Biomedically important structural information about protein molecules can be obtained by
studying the diffraction patterns that are produced when protein crystals scatter incident
radiation of an appropriate wavelength. So far, the vast majority of protein structures have
been obtained through diffraction experiments involving X-ray radiation. Typically, the
computational process that is required to determine the shape and atomic arrangement of
the molecules responsible for the observed X-ray scattering (a process known as “solving”
the structure) requires the measurement of diffraction patterns from derivative (modified)
crystals as well as native protein crystals. Suitable derivatives can be prepared by soaking
native crystals in solutions containing atoms of heavy elements like mercury or by using
genetic engineering to introduce selenium atoms into the protein molecule.
However, not all protein structures can be solved using existing X-ray diffraction techniques.
The goal of the HFSP project is to develop new methods that use neutron radiation. An
important difference between X-ray and neutron diffraction involves the scattering from
hydrogen atoms. Hydrogen is normally found in nature as the isotope protium, but a small
percentage of hydrogen atoms are present as the alternative isotope, deuterium. These two
isotopes scatter X-rays the same way, but neutrons are scattered differently. This difference
can be used as the basis for making ideal derivatives that provide the information needed to
solve protein structures.
Our project, which involves collaborators in Europe and Asia, fits the international requirements
of the HFSP Organization very nicely. Together, my collaborators and I will devise
practical methods for exploiting the differential neutron scattering of the hydrogen isotopes,
and we will test these methods by applying them to three protein structures of varying
complexity. First, a team led by Dr. Alberto Podjarny at the Institut de Génétique et de
Biologie Moléculaire et Cellulaire (Illkirch, France) will develop technology for deuterating
selected parts of protein molecules. Next, Professor Nobuo Niimura at the Japan Atomic
Energy Research Institute (Tokai, Japan) will optimize and perform neutron diffraction
experiments. Finally, in Buffalo, Dave Langs and I will develop and apply new mathematical
techniques for analyzing neutron diffraction data in order to find molecular structures. We
are very excited by the new and totally unanticipated advances, only dimly foreseen at this
time, which this approach may yield.
Dr. Herbert A. Hauptman
President and Nobel Laureate
Respectfully submitted,
Herbert A. Hauptman, Ph.D.
President
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Chief Executive Officer’s Message A Historical Perspective — 50 Years of Basic Biological Research ...
Dr. George T. DeTitta
Executive Director and
Chief Executive Officer
Last year was a period of tremendous growth at Hauptman-Woodward.
Our physical facility’s growth is easily apparent and readily appreciable in terms of its
visual beauty and its relatively luxurious functionality. What may be less overt to a casual
observer is the impact the physical structure will have on everything that occurs at HWI.
The catalyst for the building’s conception and design was the need to take a calculated
risk to ensure the organization’s future success. We were in a potentially precarious
moment in our history. If we stayed the same size and grant dollars dissipated, the very
health of the organization would be jeopardized. If we could grow our staff and our
accompanying grant dollars, it would bolster HWI’s long-term fiscal stability.
But to grow the people, we needed to grow the building. We have completed the
building and have actively begun step two – growing the staff. The first phase of recruitment
has been completed and we have welcomed three new scientists. We are in the
second phase of recruitment. The recruitment strategy is a multi-year plan in which we
will welcome two to three new scientists and their staff each year. These new hires represent
core growth and also will offset the organization’s naturally-occurring attrition.
This world-class facility broadens our grant opportunities and potentially increases the
feasibility of grant success. Funding streams that once would have been beyond our
reach may now be more viable options for HWI. Already we are seeing tangible results.
As part of the Protein Structure Initiative, we led a consortium of seven institutions to apply
for an NIH (National Institutes of Health) grant and it was funded. The five-year grant will
bring in $17.1 million – more than $14 million of which will be allocated to the three New
York institutions (HWI, the University of Rochester and Cornell University). This single grant
has proven the “build it and they will fund” philosophy. In fact, this one grant – an application
which would not have been feasible without HWI’s new facility – will bring to New
York new federal dollars which are equivalent to the contribution made by the Empire State
Development Corporation to the building of our new facility. In other words, the dollars
invested by New York State already have been “paid in full” – and we anticipate the return
on investment will continue to reap benefits to the local and state economy.
And that does not even address the work that grant is supporting. Those dollars are
funding the Center for High-Throughput Structural Biology, making us one of ten
nationally funded centers that are part of the National Institute of General Medical
Sciences (NIGMS) Protein Structure Initiative (PSI). The PSI is a national effort to
assemble a large collection of protein structures in a high-throughout operation. The
long-range goal of the PSI is to make the atomic-level structures of most proteins easily
obtainable from their corresponding DNA sequences. This knowledge could help
researchers better understand the function of proteins, learn how altered structures
can contribute to disease and identify new targets for drug development.
The center also will serve as a structural biology research resource for the greater
biological community, forming a partnership with biologists and structural biologists
that will make the high-throughput crystallization and cryopreservation pipeline
available as a community resource. The facility already has served the needs of more
than 600 structural biologists conducting more than 11 million experiments on more
than 7000 protein samples. This project will extend that pipeline to include optimization
and growth of frozen, mounted, diffraction-quality crystals ready for structural analysis.
It will offer the rapid benefits of this technology to a larger biological community.
The 2005 year was a monumental time in many ways for HWI and it closed our first
50 years. I have complete confidence that the next 50 years will be a time of even
greater growth and discovery for both HWI and the greater scientific community.
Sincerely,
For the past fifty years, the scientists and staff members at the Hauptman-Woodward Medical Research Institute
have invested their talent and careers in their penultimate passion – using the sciences to find solutions to the
puzzles of the human body.
The science which is done at Hauptman-Woodward unlocks the most basic biomedical mysteries by discovering the
three-dimensional structures of proteins. This information is the master key to the next steps in scientific research.
The work that is and has been done here is used everyday by basic research scientists, clinical researchers and
physicians all over the world.
But where did it all begin ?
This Institute was founded in 1956 as the Medical Foundation of Buffalo (renamed
the Hauptman-Woodward Medical Research Institute in 1994). The organization
resulted from the combined efforts of Dr. George F. Koepf who provided the vision
and Helen Woodward Rivas who provided generous financial support.
Dr. George F. Koepf was a physician and endocrinologist whose interest in
research began during his second year at medical school and continued at Johns
Hopkins University. After leaving Johns Hopkins, he returned to practice medicine
in Buffalo and became a founding member of the Buffalo Medical Group. One of
his patients, Helen Woodward Rivas, expressed a great interest in funding a
medical research effort in Buffalo. Through her $3 million gift, the Medical Foundation
of Buffalo (MFB) came into being.
The first site of MFB was a carriage house located at the corner of Delaware
Avenue and W. Utica Street. In 1960, plans were in progress to expand these
facilities when a fire ravaged the entire building. The building itself was devastated,
but most of the research records were salvaged.
Dr. George F. Koepf
Helen Woodward Rivas
George T. DeTitta, Ph.D.
Executive Director and Chief Executive Officer
Medical Foundation of Buffalo 1956 - 1960 Carriage House, 1014 Delaware Avenue
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HWI Focuses on Crystallography
A new four-story building was constructed at 73 High Street with the help of matching
funds from the National Institutes of Health (NIH). This new facility opened in 1963.
The Medical Foundation of Buffalo which was housed at 73 High Street from 1962 - 2005 was renamed the
Hauptman-Woodward Medical Research Institute in 1994 in recognition of the symbiotic relationship between science and philanthropy
Our Nobel Laureate
Dr. Herbert A. Hauptman, HWI President,
accepts the Nobel Prize in Chemistry in 1985 from
His Majesty King Carl XVI Gustaf of Sweden
Foremost among the crystallographers who were leading this groundbreaking
era of scientific discovery was Dr. Herbert A. Hauptman. In
1985, the Institute was in the international scientific limelight when
Hauptman became the first mathematician to receive the Nobel Prize
in Chemistry. He shared this honor with Dr. Jerome Karle of the Naval
Research Laboratory in Washington, D.C. The prize was awarded for
outstanding achievement in the development of new mathematical
methods for analyzing crystallographic diffraction data. The
techniques pioneered by Hauptman and Karle have since been used
by crystallographers throughout the world to study thousands of
molecules whose structures were previously inaccessible.
Hauptman continues to build upon the foundation provided by his
prize-winning work. He and his colleagues devised a method called
"Shake-and-Bake" that extends the range of direct methods to
include much larger structures such as proteins. To honor the distinction
Hauptman brought to our organization and to recognize our
benefactor, Helen Woodward Rivas, the organization changed its
name to the Hauptman-Woodward Medical Research Institute, Inc. in
1994. While the change was made to honor these two individuals, it
also was a conscious effort to overtly recognize the importance of the
partnership that exists between science and philanthropy.
Dr. William L. Duax (photo circa 1970s)
H. A. Hauptman Distinguished Research Scientist
In the late 1960's, led initially by Research Director Dorita
Norton and later by Dr. William Duax, the Institute’s research
began to focus on the science of crystallography. The development
of improved drugs requires knowledge of the threedimensional
shapes of the biochemical substances involved in
disease processes, and crystallography employs an experimental
technique known as diffraction to discover vital information
about molecular structure. Therefore, the leadership team
headed by President Koepf set the goal of establishing a
world-class crystallographic laboratory in Buffalo. During the
next decade, more than a dozen crystallographers were hired
– a hiring boom which achieved a critical mass with expertise
in all aspects of the science. Research projects involving
structural studies of steroid and thyroid hormones, prostaglandins,
peptide antibiotics, and naturally-occurring opiates were
initiated during this period. The Institute’s scientists were among
the first in the world to recognize the importance of threedimensional
structure to explain biological function at the
molecular level and to communicate these ideas to the biological
and endocrine communities.
Hauptman and Mrs. Edith Hauptman
celebrate the news of the Nobel Prize
HWI Founder Dr. George Koepf congratulates Hauptman
Hauptman at the press conference where
news of his impending receipt of the
Nobel Prize was shared with the world
HWI staff members salute our Nobel winner,
(left to right) Dr. Charles M. Weeks, senior research scientist, Hauptman,
Steven Potter, computer systems manager, Dr. William L. Duax, H. A.
Hauptman Distinguished Research Scientist
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The Changing Nature of Our Work
During the 1970s, HWI scientists primarily directed their studies
toward relatively small molecules such as drugs and hormones
containing 100 atoms or less. However, during the 1980s and
1990s, a variety of technological advances permitted crystallographers
to focus their attention increasingly on the macromolecular
entities (protein, DNA, RNA) which carry out most life
processes, and to observe the actual interactions of drugs with
these larger molecules. The hormone insulin and several steroid
synthesizing and metabolizing enzymes are examples of proteins
studied by HWI crystallographers during those decades.
In 1999, a grant from the John R. Oishei Foundation, coupled
with an award from the Margaret L. Wendt Foundation and
support from many other foundations, corporations and
individuals provided the Institute with the means to establish a
Structural Biology Center. All aspects of structural research -
from isolating the gene that codes for a protein of interest to
applying the latest crystallographic methods - are now possible
here. Through the Structural Biology Center, HWI scientists were
able to create new and improved crystal growth methods
including the development of a novel and patented highthroughput
robotic crystallization technique for expediting and
optimizing the crystallization process. The technique which was
developed by HWI’s current Chief Executive Officer and Executive
Director George T. DeTitta and Research Scientist Joseph R.
Luft has vastly improved the efficiency of conducting crystal
growth experiments. HWI now serves as a resource for
scientists from all over the world and the facility now performs
more than 3.5 million experiments per year.
Dr. Robert H. Blessing, (left) senior research scientist and
Dr. George T. DeTitta, executive director and CEO have
collaborated for more than three decades (photo circa1970s)
top, Joseph R. Luft, research scientist,
center, Jennifer Smith, research associate
bottom, Christina Veatch, research associate
This photo depicts the construction of a molecular model based upon the application of
Dr. Herbert A. Hauptman’s original Direct Method Techniques as done in the 1970s
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Future Growth Requires Physical Growth
As HWI reached the 21st Century, future planning continued to be a focus. HWI
became a founding partner of the Buffalo Niagara Medical Campus (BNMC),
created the Department of Structural Biology which is part of the University at
Buffalo School of Medicine and Biological Sciences, and added new leadership; Dr.
Herbert A. Hauptman was named President, Dr. George T. DeTitta was appointed
Executive Director and CEO and Dr. Walter A. Pangborn was appointed Executive
Vice President. With a solid leadership team in place, the need to recruit new
scientists became increasingly evident. As HWI added four new scientists and
planned to recruit even more, expansion options at our High Street facility were
investigated. After reviewing many alternatives, it became clear that a new research
building would be needed.
As the University at Buffalo and the Roswell Park Cancer Institute began plans to
construct new facilities, HWI joined its collaborative partners to form the Buffalo Life
Sciences Complex. Located in the heart of the BNMC, HWI was able to select a site
adjacent to the UB and RPCI sites for our new building. Cannon Design and
architect Mehrdad Yazdani were selected to design a research and office complex
that supported HWI’s collaborative and open culture. Construction of the new
Structural Biology Research Center began in August 2003, through the support of
the state and federal government, local foundations, community leaders, and
individuals, as well as financing through KeyBank.
The new facility held its grand opening in May 2005 and has begun the next stage
in HWI’s future growth plan. So where does HWI go from here …
The Future – Forging Into The Next 50 Years
It is clear that HWI has been a scientific leader throughout its 50 year history.
The next fifty years will be a continuation of a tradition of excellence in the sciences. Our new and existing scientists
will continue to build the foundation needed to solve the mysteries that stand in the way of better medications, a
better quality of life and better overall health.
A New Building, A New Era
HWI’s new state-of-the-art Structural Biology Research Center is home to our world-class staff and showcases the
research that goes on within its laboratories. This signature building provides HWI with the physical accommodations
necessary to recruit new scientists, expand research initiatives and build upon the group’s collaborative nature.
The design is comprised of a rectilinear translucent laboratory block, a curved office wing and a three-story atrium
which join those two components. The combination of the purity of the square and the fluidity of the curve reflects
the dynamic coexistence of the intuition and reason which is at the very core of all scientific research: the curved
office section symbolically houses the scientific mind while the square lab holds the tools.
While laboratory buildings have traditionally placed researchers’ offices adjacent to their individual labs, here the offices
have been consolidated in an architecturally distinct section of the building, joined to the laboratory block by the atrium.
Scientists and other staff members must continually move between the laboratory and office blocks which encourages
interaction and serves as the building’s social hub where staff members can engage in spontaneous, informal conversations
and debate. The curve of the office block provides a more private, contemplative setting for the staff.
The building serves as the gateway to the new Buffalo-Niagara Medical campus, a major urban initiative that
houses Buffalo General Hospital, Roswell Park Cancer Institute and the New York State Center for Excellence in
Bioinformatics and Life Sciences, and is a key component to its overall goal of establishing a world-renowned
research center in Buffalo. The 73,000 square foot new building doubles the size of the Institute’s former home and
is considered instrumental in attracting new scientists and funding to the Institute.
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New Scientists To Spur Ongoing Growth
Today’s Scientists Training the Scientists of Tomorrow
A key priority for HWI’s future success is the recruitment of new scientists and the identification
of a funding mechanism to underwrite the recruitment process. The recruitment
effort is being conducted in stages with the goal of doubling our staff over the next seven
years at a rate of two to three scientists per year.
Our current strategy calls for the future recruitment of two senior scientists, four mid-level
scientists and five junior scientists.
The first recruitment stage has been completed and three new scientists are in place and
building their lab staffs. The second recruitment stage has begun and candidates
currently are being considered.
Each recruit has individual needs. In general, those needs include start-up support for a four
to six year period, technical support for a like period, custom laboratory equipment, graduate
student support, and biological supplies. The commitment on our part is to the time period
necessary (the start-up support period) to perform the preliminary experiments needed to
request funding at the federal level by a major sciencefunding
agency such as the National Institutes of Health
and the National Science Foundation.
An investment in Hauptman-Woodward is not only
good for the Institute, but also for Western New York.
The investment of $13.3M in Hauptman-Woodward -
the overall financial investment for the new recruits -
has the potential for an effective return of $72M over
the 2006-2015 period in economic output for the
Buffalo Niagara region.
Although HWI is primarily a research institute, we strive to make a unique contribution to improving science literacy and
encouraging young people to pursue science careers. Our research investigators offer hands-on and state-of-the-art experiences
in the area of health problems and research - experience which helps to support the student’s decision to pursue a
health-related profession. This is particularly important due to the waning numbers of young people who are interested in
science careers, specifically among minority populations. The goal also is to encourage the students to remain in or return to
Western New York upon completion of their college degree.
The educational outreach has three primary components – graduate students, summer students and high school outreach.
Graduate Students: Our scientific staff members serve as research and academic faculty members at the State University of
New York at Buffalo and the Roswell Park Cancer Institute. The Department of Structural Biology for the School of Medicine at
UB is now housed at Hauptman-Woodward, with our scientists serving as faculty and mentors for graduate students.
Summer Students: Apprentices are selected for our summer student program from college undergraduate applicants who are
permanent residents of Western New York. We are particularly interested in attracting talented students majoring in the
sciences at the undergraduate, graduate or professional level, to complement their educational training with an experience in
an HWI laboratory. We also have been able to attract financially-needy students. The students obtain hands-on training in a
working lab under the supervision and guidance of a principal research scientist. Dr. Herbert A. Hauptman also is available
to meet with and share his knowledge of the sciences and career opportunities in biomedical research. Each apprentice is
involved in a scientific project using state-of-the-art equipment in the fields of molecular biology, methods development, crystal
growth, and x-ray diffraction to find ways to prevent and treat diseases such as cancer, breast cancer, diabetes, AIDS, thyroid
disorders, SARS and Alzheimer’s disease.
Dr. Daniel T. Gewirth, senior research scientist
Albert Reger, structural biology student, prepares to collect x-ray diffraction data.
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Pioneers of Science High School Educational Outreach: Western New York has a long, proud and
rich history of producing leaders in many branches of science. To salute some of these individuals and
to focus local and national attention on the high caliber of scientific work that is thriving here,
Hauptman-Woodward hosts our Pioneers of Science Conference. The event is made up of two separate
programs: one, a morning lecture and series of workshops for area high school science students and
the second, a dinner and awards presentation gala. The objective of the conference is to inspire high
school students through the achievements and accomplishments of leading Western New York
scientists. They have the opportunity to meet in small workshop sessions led by each of the honored
scientists. The students also will learn of current and future research and career opportunities. The
target audience for this conference is science-inclined high school students currently attending Erie
County public and private schools. Approximately 200 students participate in this program.
A New Public Science-Math High School: HWI is participating in the development of a new high
school that will be part of the Buffalo Public School system where the concentration for the students will be
focused primarily on the sciences and mathematics. The goal is for the school to open in the fall of 2006.
Overall, HWI is strategically moving into the next 50 years. We are in a new home, growing our HWI
family with new scientists, technicians and support staff and we are continually expanding the boundaries
into new arenas of science.
The Science at the Hauptman-Woodward Medical Research Institute
Structural biology is the study of the shapes of molecules, such as proteins,
that exist in the body. Each protein has a distinct shape and function and
is very often involved in one or more diseases. Knowing the threedimensional
structure of these proteins enables scientists to understand
how they work and can allow them to develop new medications to treat
disease. At Hauptman-Woodward, our scientists specialize in structural
biology and the use of x-ray crystallography.
What is the Process?
The structural biology process is often complicated and time consuming,
but each step is critical to understanding disease and to developing
new ways to treat, prevent, and possibly cure them. There are six
distinct steps that take place in the structural biology process at HWI.
1.
Protein production and purification: In this stage, genes
implicated in diseases are identified, isolated, cloned, and
introduced into protein expression systems. Finally, these proteins
are over-expressed in various systems to determine the highest
level of protein production, and purified using high-pressure
liquid chromatography to achieve greater than 99 percent purity.
Breast cancer drug target
2.
Crystal growth: Once a purified protein is obtained, crystals are
produced, using high-throughput crystallization, a technique
created by our scientists that performs 1536 experiments at a
time using a robotic system, and which records the results in a
database for future access.
3.
4.
X-ray diffraction: After crystals of adequate diffraction quality are
obtained, they are mounted on a diffractometer or brought to a
synchrotron facility where a unique diffraction pattern is produced.
Analysis of diffraction data: A computer analysis of the diffraction
data is performed that allows the location of atoms in the
molecules to be determined. An electron density map is then
created and viewed, using state-of-the-art computer graphics.
Methods developed by Dr. Hauptman are used at this stage.
AIDS-related research
5.
Analysis of molecular structure and function: Scientists are then
able to view the three-dimensional structure that has been
determined to understand how structure guides biological function.
Pioneer of Science honoree Edith Flanigen explains her research to local high school students at the
Pioneers of Science student symposium.
Structure-Based Drug Design: By studying the structure and
function of molecules we can design new medications that are
more selective and effective in the fight against diseases. The
AIDS drugs that have proved to be so successful in extending
patients' lives were designed using the three-dimensional
Protein essential for cell proliferation
and growth
structure of the AIDS protease.
12 13
6.

What Are The Tools Used to Get the Job Done?
There are a wealth of laboratory resources available to HWI scientists and graduate students. Among them are:
The Molecular Biology Lab - Important disease-related genes are identified and isolated. The gene that codes for the protein
of interest is cloned and introduced into an appropriate cell line. The molecular biology laboratory is the starting point for
most structural biology projects. These projects involve the study of protein molecules, most of which are present in the body
only in minute quantities. The instructions for making proteins are found within the genes, the hereditary material that is
composed of deoxyribonucleic acid (DNA), contained in the chromosomes present in each cell, and passed from one generation
to the next. The main objective of the molecular biology lab is to manipulate the DNA that codes for a protein of interest
in ways that make it possible to produce the large quantities of protein required for structural studies.
The Protein Production & Purification Lab – Cells are cultured under conditions that support large-scale production
(expression) of the desired protein. The cells are then disrupted, and techniques such as electrophoresis and column chromatography
are used to separate the target protein from other (contaminating) proteins on the basis of properties such as charge
and size. Many molecular biologists and biochemists can use nanogram quantities of protein for their experiments. In order
to carry out successful structure determinations, however, crystallographers require a million times more protein. HWI’s protein
production and purification laboratory has been designed for the purpose of producing proteins on a 1-100 mg scale and to
achieve 99 percent purity for crystallization and subsequent structural studies.
The Diffraction Lab – Single crystals are exposed to X-rays to create a diffraction pattern that is recorded electronically by an
area detector. In most cases, diffraction data are also measured using the high intensity X-ray beams available from synchrotron
radiation sources at Cornell University and the Brookhaven and Argonne National Laboratories.
The Crystal Growth Lab – Here the purified protein sample is combined with crystallization solutions to grow high quality
single crystals. Automated, high-throughput screening techniques are applied to micro samples in order to identify suitable
growth conditions. The high-throughput crystallization laboratory at HWI houses state-of-the-art facilities for screening conditions
suitable to grow the high-quality single crystals required for molecular structure determination by X-ray diffraction. As
currently configured, the lab has the capacity to evaluate as many as 200 new samples each month. A large number of experiments
(1536) can be set up within minutes after a protein sample is received, thereby reducing the chance for degradation.
The approach used for screening growth conditions is to incubate, under paraffin oil, small aqueous aliquots of the sample
protein with chemical mixtures (called ‘cocktails’) that are designed to induce a state of supersaturation. The protein molecules
will then be driven out of solution and, under proper conditions, crystals will form. The high-throughput experiments are set
up using Robbins Scientific Tango pipetting robots. Two custom-made photomicrographic reader tables, that can accommodate
as many as 28 crystallization plates each, are used to document the results with a digital camera. Digital images are
recorded and the plates are stored in temperature-controlled incubators.
The crystallization lab also contains all the equipment needed to optimize crystal size and quality once the initial conditions
have been found. The instruments available for preparing and characterizing macromolecular solutions and crystallization
cocktails include pH meters, electronic balances, centrifuges, refractometers, a spectrophotometer, a viscometer, and an
osmometer. Two DynaPro temperature-controlled dynamic light scattering instruments are available to make measurements
of solution homogeneity. These measurements are useful for predicting the likelihood that crystals will form from a solution.
The Computational Lab – The diffraction data are analyzed to find the atomic positions using software including the program
SnB written at HWI. With the help of three-dimensional computer graphics, electronic models of the protein structure are then
constructed. Finally, color graphics are used to depict the overall molecular architecture, as well as the locations of chemically
and biologically important regions.
The crystal growth lab uses high-throughput screening techniques to identify suitable growth conditions.
Eric Drake, research associate, works on one of the many steps involved in protein purification.
Angela Lauricella, research associate, is pictured (bottom right) working in the crystal growth lab.
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What is Being Done in Research and Development?
Every day HWI scientists contribute to the body of knowledge and understanding of structural biology research. Over the
past five years, HWI scientists have made many significant achievements, including:
Initiating research into emerging infectious diseases
In 2003, when Severe Acute Respiratory Syndrome (SARS) emerged as a highly infectious and deadly disease, HWI
scientists partnered with ZeptoMetrix, a local biotechnology company, and Virionyx, a biopharmaceutical company in New
Zealand to research the virus and help develop a passive vaccine.
HWI’s work on this project is two-fold; we are supplying our collaborators with purified proteins for use in creating a passive
vaccine and we are determining the structures of individual proteins that form the SARS-CoV replicase complex in order to
use the structures as a foundation to understand their biological functions. The hope is to also apply this research strategy
to other emerging diseases, such as West Nile Virus, Dengue Fever, and the common cold.
Working to create safer arthritis medications
Recently, new arthritis medications have been taken off the market due to adverse side effects. HWI scientists are working to
provide insight into how non-steroidal anti-inflammatory drugs (NSAIDs) affect the inflammatory process. This also may
lead to development of new medications to treat rheumatoid arthritis, osteoarthritis, and other inflammatory diseases, with
fewer side effects.
Developing new antibiotics for infectious diseases
In recent years, infectious diseases have become more difficult to treat with antibiotics, as antibiotic resistance becomes an
increasing public health problem. HWI’s research will study the structure and function of certain enzymes that synthesize
antibiotics, perhaps allowing the engineering of these proteins for the production of a new generation of drugs.
Expanding our methods research
Methods research has always been an important part of the research that takes place at HWI. For more than 30 years, our
scientists have been working to build upon Dr. Herbert A. Hauptman's Nobel Prize winning methods.
A project to develop a systematic methodology for resolving the phase problem in crystallographic computing is underway.
This work promises to lay the foundation for a new generation of crystallographic computing systems that will reveal the
structure of millions of substances that are important in the understanding of life, materials, science, and drug design.
In addition, Hauptman and his team are working on the development of Neutron Shake-and-Bake (NSnB), an algorithm for
the solution of the phase problem when only neutron diffraction data are available. Hauptman is collaborating on this project
with colleagues from the Japan Atomic Energy Research Institute (JAERI), Ibaraki University in Japan, and the Institut de Genetique
et de Biologie Moleculaire et Cellulaire (IGBMC) in France. This project has been awarded a prestigious multi-continental
three-year grant which will open the pathway to new understanding of the use of neutron diffraction methods.
James Pace, research associate, observes the protein gel of an
AIDS - related enzyme being studied in Dr. Vivian Cody’s lab.
Dr. David A. Langs, senior research scientist, reviews one of the many
abstracts available in the Grigg-Lewis Science Library on the third floor of HWI.
Dr. Michael G. Malkowski, research scientist, is shown here
preparing crystals for synchrotron data collection.
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The People of the Hauptman-Woodward Medical Research Institute
Our greatest asset of all is our people. Hauptman-Woodward is fortunate to be home to some of the
most creative minds in science today and has the distinction of offering an investigator-initiated
approach that allows our scientists to translate their passion for their work into their everyday experiences.
The scientific team is supported daily by talented individuals who serve on our boards and a
staff which includes individuals with a wide range of talents and experiences. Each employee at
Hauptman-Woodward plays a role in ensuring the organization’s current and future successes.
Board of Directors and Officers
Donald A. Hess
(Chairman)
Paul J. Koessler
(Vice Chairman)
Herbert A. Hauptman, Ph.D.
(President)
George T. DeTitta, Ph.D.
(Executive Director and CEO)
Walter A. Pangborn, Ph.D.
(Executive Vice President)
James R. Biltekoff
(Treasurer and Chairman,
Finance Committee)
Elizabeth S. Mitchell
(Secretary)
Lisa Foti Milk, CPA
(CFO and Assistant Treasurer)
Adelbert Fleischmann
(Co-Counsel)
Michele O. Heffernan
(Co-Counsel)
Cynthia Ambres, M.D.
Richard Aubrecht
Norbert A. Bennett
Alan L. Dressler
Robert Glenning
Christopher T. Greene
David C. Hohn, M.D.
Robert J. A. Irwin
William L. Joyce
Elizabeth C. Marks
Lewis D. McCauley
Peter T. Ostrow, M.D., Ph.D.
Patrick Reilly
Richard W. Shaughnessy
Walter F. Stafford, III, Ph.D.
Charles M. Weeks, Ph.D.
(Chairman,Scientific Governance Council)
Emeritus Directors
Alan P. Bagley
Thomas R. Beecher Jr.
James R. Kanski, M.D.
Charles A. Martin, Jr.
Donald F. Newman
Albert J. Wright, III
Foundation Board
Christopher T. Greene
(Chairman)
Elizabeth S. Mitchell
(Vice Chairman)
George T. DeTitta, Ph.D.
(Executive Director and
Chief Executive Officer)
Elizabeth C. Marks
(Secretary)
James R. Biltekoff
(Treasurer)
Nancy L. Dowdell
Alan L. Dressler
Christopher G. Gibas
Eva M. Hassett
Herbert A. Hauptman, Ph.D.
Donald A. Hess
Paul J. Koessler
Richard W. Shaughnessy
Institute Leadership
Herbert A. Hauptman, Ph.D.
President
George T. DeTitta, Ph.D.
Executive Director and
Chief Executive Officer
Walter A. Pangborn, Ph.D.
Executive Vice President
Donald A. Hess
Chairman, Board of Directors
Distinguished Research Scientists
William L. Duax, Ph.D.
Herbert A. Hauptman, Ph.D.
Principal Research Scientists
Vivian Cody, Ph.D.
George T. DeTitta, Ph.D.
Jane F. Griffin, Ph.D.
Walter A. Pangborn, Ph.D.
Senior Research Scientists
Robert H. Blessing, Ph.D.
Daniel T. Gewirth, Ph.D.
Debashis Ghosh, Ph.D.
David A. Langs, Ph.D.
Vladimir Pletnev, Ph.D.
Charles M. Weeks, Ph.D.
Research Scientists
Barnali Chaudhuri, Ph.D.
Andrew M. Gulick, Ph.D.
Joseph R. Luft
Michael G. Malkowski, Ph.D.
L. Wayne Schultz, Ph.D.
Edward H. Snell, Ph.D.
Timothy C. Umland, Ph.D.
Hongliang Xu, Ph.D.
Emeritus Scientists
Dongyao Guo, Ph.D.
Yoshio Osawa, Ph.D.
G. David Smith, Ph.D.
Research Associates
Jessica Kocsis
Angela Lauricella
Tracy Lloyd
James Pace
Carleen Pope
Jennifer Riggie
Meriem Said
Jennifer Smith
Jesse Sundlov
Christina Veatch
Jennifer Wolfley
Postdoctoral Fellows
Chien-Chung Chang, Ph.D.
Stacey Gulde, Ph.D.
Qilong Mao, Ph.D.
Mary Rosenblum, Ph.D.
Manish Shah, Ph.D.
Research Aides
Sanjay Connare
Adam Krol
Lynn Nyazika
Structural Biology Students
William Bauer
Danielle Campanaro
Robert Huether
Zachary Miknis
David Parish
Albert Reger
Administration
Jean E. Gallmeyer
Mary Lou Grauer
Dorothy Grimes
Deanna Hefner
Computer Systems
Daniel P. Degnan
Geoffrey Franks
Raymond M. Nagel
Stephen A. Potter
Naimesh Shah
Max Thayer
Development
Laurie Elliott Krajna
Facilities Aide
Darlene Miller
Financial Management
Lisa Foti Milk, CPA
Michael Guerra
Anne Kent
Kristina Rogers
Deena Vanderbosch, CPA
Graphic Design
Gloria J. Del Bel
Melda Tugac
Public Relations and
Government Affairs
Tara A. Ellis
Research Associates
Margaret Cegielski
Robin Culp Kempkes
Han-Chun Cheng DeFedericis
Eric Drake
Mary Erman
Wendy Franke
Leah Gambino
Select members of HWI’s Board of Directors and Officers, as well as members of
Jennifer Griswold
HWI’s Foundation Board, are pictured above.
Jillian Kaczmarek
Dorothy Grimes and Kristina Rogers,
administration and financial management team members
18 19

HAUPTMAN-WOODWARD MEDICAL RESEARCH INSTITUTE
STATEMENTS OF FINANCIAL POSITION*
OCTOBER 31, 2005
ASSETS
Current assets:
Cash $ 106,535
Contributions receivable, net 1,531,411
Grants receivable-capital campaign 553,594
Grants receivable-science 328,953
Prepaid Expense 16,952
Total current assets 2,537,445
Donor Highlights
We are proud to gratefully acknowledge the generous donors who support the Hauptman-Woodward Medical Research Institute,
Inc. Your philanthropy enables our dedicated scientists to unlock the mysteries of life-threatening and debilitating diseases which
touch the lives of people all over the world. Every new discovery at HWI will lead to new and improved treatments for patients
and will have an impact on the health of members of our international community for many generations to come.
LIFE MEMBERS
Mrs. Marjorie Buyers
Mr. and Mrs. Clinton F. Ivins, Jr.
Mrs. W. Jackson Catt
Cannon Design
Ms. Lucetta C. Knox
Mr. and Mrs. Joseph Stewart
Mrs. Erika Daley
Mr. and Mrs. G. David Koepf
Mr. and Mrs. Gilbert J. Yager
Dr. and Mrs. William L. Duax
Mr. and Mrs. Erick J. Laine
INSTITUTE BENEFACTORS ($10,000+) Mr. and Mrs. Christopher T. Greene
Mr. Norman E. Mack, II
Mr. and Mrs. Clement R. Arrison
Dr. and Mrs. Herbert A. Hauptman
Mr. and Mrs. Jeffrey B. Marsh
Arthritis Foundation
HealthNow New York Inc.
Mr. and Mrs. Durham S. McCauley
Baird Foundation
Invitrogen Corporation
Mr. and Mrs. Donald M. McClellan
Cameron Baird Foundation
Jaeckle, Fleischmann & Mugel, LLP
ONY Inc.
Investments:
Mr. Charles E. Balbach
Josephine Goodyear Foundation
Mr. and Mrs. William J. Regan, Jr.
Science 4,540,187
Edward H. Butler Foundation
Joy Family Foundation
Rigaku-MSC
Held by trustee 116,915
Community Foundation for Greater Buffalo Lifetime Health Medical Group
Mr. Richard W. Shaughnessy
4,657,102
Mr. William J. Constantine
Mr. William J. McDermott
Mr. and Mrs. Reid W. Stafford
Fixed assets:
James H. Cummings Foundation, Inc.
Mr. and Mrs. Robert L. Montgomery Jr. Mrs. Richard W. Stewart
Adele & George T. DeTitta
Rigidized Metals
The Buffalo News
Land, building and equipment, net 23,839,217
Mr. and Mrs. Charles E. Dowdell
UB Department of Government Affairs Mr. Richard R. Upton
Construction in progress 521,092
Max & Victoria Dreyfus Foundation, Inc. Walsh Duffield Companies, Inc.
Mr. and Mrs. Samuel F. Ward
24,360,309
Mr. and Mrs. Peter B. Flickinger
CRYSTAL CIRCLE MEMBERS ($1,500+) CIRCLE SPONSORS ($500+)
Other assets:
Mr. and Mrs. Thomas R. Flickinger
Applied Sciences Group Inc.
Mr. and Mrs. Charles Lee Abell
Contributions receivable, net 2,504,678
Mr. William S. Flickinger
Berger & Berger
Mr. and Mrs. Douglas T. Baker
Richard W. and Mae Stone Goode Trust Mr. and Mrs. James R. Biltekoff
Dr. and Mrs. Theodore S. Bistany
Deferred financing fees, net 744,609
Mr. and Mrs. Wilson Greatbatch
Buffalo Medical Group
Mr. and Mrs. Michael A. Brady
Assets held in charitable trust 283,974
Grigg-Lewis Foundation, Inc.
Buffalo Niagara Medical Campus
Mrs. Annette M. Cravens
Swap contract 25,473
Mr. John Cordes & Ms. Michele Heffernan Mr. and Mrs. Bruce Buyers
Mr. and Mrs. Alan L. Dressler
3,558,734
Mr. and Mrs. Donald A. Hess
Citigroup Private Bank
Mr. and Mrs. Fred Friedman
Kaleida Health
Damon & Morey LLP
Dr. Eugene L. Gaier
Total assets $35,113,590
Key Bank
Delaware North Companies, Inc.
Dr. Robert J. Genco
Seymour H. Knox Foundation
Mrs. Cynthia Doolittle
Grover Cleveland Press, Inc.
LIABILITIES AND NET ASSETS
Mr. and Mrs. Paul J. Koessler
Mr. and Mrs. Richard E. Garman
Dr. and Mrs. L. Nelson Hopkins, III
William G. McGowan Charitable Fund, Inc. Hodgson Russ LLP
Mr. and Mrs. Dean H. Jewett
Current liabilities:
Moog, Inc.
Dr. and Mrs. David C. Hohn
Dr. James R. Kanski & Dr. Genevieve Kanski
M&T Charitable Foundation
Irenaeus Foundation
Honorable and Mrs. Theodore S. Kasler
Accounts payable - operating $ 177,925
Mrs. David Oliver Smith
Mrs. Mary M. Koessler
Mr. and Mrs. Kevin T. Keane
Accounts payable - construction-in-progress 199,537
John R. Oishei Foundation
L. McCauley Trust
Dr. Kathleen Kreis
Accrued payroll and related taxes 153,633
Constance W. Stafford Charitable Trust Mr. Frank J. McGuire
Dr. Paul Kurtz
Refundable advances -
Dr. and Mrs. Walter F. Stafford, III
Dr. and Mrs. Yoshio Osawa
Mr. and Mrs. Robert Lang Miller
Capital lease obligations 6,165
Mr. and Mrs. Peter A. Vogt
Dr. and Mrs. Peter T. Ostrow
Louis P. Ciminelli Construction Co., Inc.
Margaret L. Wendt Foundation
Frances & Walter A. Pangborn
Dr. and Mrs. J. Arthur Mattern
Bonds payable - current portion 175,000
Western New York Foundation
Peter & Elizabeth C. Tower Foundation
Mr. and Mrs. E. Dennis McCarthy
Total current liabilities 712,260
PARTNER'S IN RESEARCH ($5,000+)
Roswell Park Cancer Institute
Mr. and Mrs. Peter L. McCauley
Mr. and Mrs. Henry H. Baxter, P.E.
Mr. Willard B. Saperston
Mr. and Mrs. Richard Minekime
Bonds payable 6,785,000
Mr. and Mrs. Walter Constantine, Jr.
Sevenson Environmental Services
Dr. and Mrs. Herman S. Mogavero, Jr.
Dr. and Mrs. Angelo M. Fatta
Miss Gail S. Weymouth
Nixon Peabody LLP
Accrued swap liability -
Mrs. Whitworth Ferguson Jr.
CIRCLE PATRONS ($1,000+)
Mr. and Mrs. Charles E. Paul
Total liabilities 7,497,260
Ms. Edith M. Flanigen
Mr. and Mrs. Thomas R. Beecher, Jr.
Mr. and Mrs. George F. Phillips, Jr.
Jane & Richard Griffin
Dr. Harold Brody
Miss Frances M. Rew
Mr. and Mrs. Donald J. Holzman
Mr. and Mrs. Maxwell Caulkins
Mr. and Mrs. Edward B. Righter
Net assets:
Mr. and Mrs. Vincent Lawless
Mr. and Mrs. Theodore Chertoff
Ms. Fannette Sawyer
Unrestricted 19,681,720
Legg Mason Investment Counsel
Dr. Vivian Cody
Shuman Family Foundation
Little-Kittinger Foundation
Mr. and Mrs. Richard W. Cutting
Mr. and Mrs. Donald Smith
Temporarily restricted 7,290,141
Louis S. & Molly B. Wolk Foundation
Mr. and Mrs. James Degen
Mr. and Mrs. John G. Stanley, Jr.
Permanently restricted 644,469
Mr. and Mrs. John A. Mitchell
Mr. and Mrs. Thomas C. Detwiler
Dr. Richard A. Stockton, Jr.
Total net assets 27,616,330
Scott, Danahy & Naylon Co., Inc.
Donald Davis Foundation
United Way of Buffalo & Erie County
Mr. and Mrs. Douglas G. Swift
Mrs. Jeanne C. Eaton
Mr. James M. Wadsworth
Total liabilities and net assets $35,113,590
Verizon
Mrs. Marion Flemming
Mr. and Mrs. John N. Walsh, Jr.
VIYU Foundation
Mr. Charles J. Hahn
Mr. and Mrs. Albert J. Wright, III
ZeptoMetrix Corp
Mrs. L. Nelson Hopkins, Jr.
CIRCLE MEMBERS ($250+)
* This information has been taken from the audited financial statements of
Hauptman-Woodward Medical Research Institute for the fiscal year ending October 31, 2005
DIAMOND CIRCLE MEMBERS ($2,500+)
Mr. and Mrs. Norbert A. Bennett
Mr. and Mrs. Robert J. A. Irwin
Mr. and Mrs. Richard B. Adams
Mr. and Mrs. Stuart H. Angert
20 21

CIRCLE MEMBERS ($250+)
Mrs. Herbert K. Astmann
Mr. and Mrs. Bruce Baird
Mr. and Mrs. William Baird Irwin
Mr. and Mrs. C. Teo Balbach
Mrs. Wavel H. Barber
Dr. and Mrs. Allen Barnett
Dr. and Mrs. Howard W. Benatovich
Mrs. Janice C. Boneberg
Dr. and Mrs. G. Richard Braen
Mr. and Mrs. A. Watson Bray
Buffalo Hearing & Speech Center, Inc.
BuffLink, Inc.
Mr. and Mrs. Gary M. Burger
Mr. and Mrs. Hazard K. Campbell
Mrs. Peter A. Casagrande
Mrs. William J. Conners, III
Mr. and Mrs. John R. Connolly
Mr. and Mrs. Robert W. Constantine
Drs. Philip & Marguerite Coppens
Mr. and Mrs. James L. Crane, Jr.
Mr. and Mrs. Arthur W. Cryer
Mr. and Mrs. Thomas H. Danforth
Mr. and Mrs. Michael Day
Dr. and Mrs. Peter C. Dow
Dr. Edward G. Eberl
Mr. and Mrs. Adelbert Fleischmann
GentCorp Ltd.
Ms. Coleta A. Glass
Dr. Kenneth W. Gross
Miss Deanna Hefner
Mrs. Ann Holland Cohn
Mr. and Mrs. Hartley Frank Hutchins
Mr. and Mrs. John L. Kirschner
Mr. and Mrs. Peter G. Klein
Mr. and Mrs. Charles F. Kreiner, Jr.
Dr. Chari Briggs & Mr. Joel Krenis
Dr. and Mrs. Jack Lippes
Mr. and Mrs. Thomas D. Lunt
Mr. and Mrs. Chester L. Mais
Mr. and Mrs. Theodore E. Marks, III
Mrs. Walter E. Matt
Mr. and Mrs. Richard E. McGill
Ms. Carol McRae
Mr. and Mrs. Clarke H. Narins
Mr. and Mrs. Reginald B. Newman, II
Dr. and Mrs. James P. Nolan, Jr.
Drs. Dean & Donna Orman
Ms. Greta Pangborn
Dr. and Mrs. John H. Peterson
Mrs. Marjorie M. Plumb
Premier Group
Dr. and Mrs. Theodore C. Prentice
Mr. and Mrs. Patrick Reilly
Mr. and Mrs. Wayne R. Reilly
Dr. Avery A. Sandberg
Mr. and Mrs. Lowell Shaw
Dr. and Mrs. Alfred M. Stein
Ms. Mimi Swados
Mr. and Mrs. Brian K. Townson
Mr. and Mrs. Jacob E. Trautman
Ms. Melda Tugac
U-C Coatings Corporation
Dr. and Mrs. James F. Upson
Mr. and Mrs. Hugh M. Van Alstyne
Mr. and Mrs. Richard H. Wadsworth
Mrs. Nancy S. Warner
Ms. Barbara Culliton Waterfall
Mr. and Mrs. Frank P. Wilton
Mr. and Mrs. Wayne D. Wisbaum
WomenStories
Mrs. Preston Wright
Mr. Arthur J. Ziffer
Mr. and Mrs. C. Richard Zobel
SUPPORTERS ($100+)
Dr. and Mrs. Mark W. Ackley
Mr. and Mrs. Robert J. Adams
Mr. and Mrs. J. Keith Alford
Ms. Anne Allan
Mr. and Mrs. Gordon P. Assad
Mr. and Mrs. Virgil J. Austin
Mr. and Mrs. Vincent B. Barrett
Mr. and Mrs. John Barry
Mr. and Mrs. Rudolf L. Bauer
Dr. and Mrs. Robert A. Baumler
Ms. Ellen W. Baxter
Mr. Richard G. Berger
Mr. and Mrs. Edward Bialek
Mrs. Betty Brady
Mr. Brian P. Brady
Mr. and Mrs. Thomas H. Brown
Mr. Richard C. Bryan
Mr. and Mrs. David Burnell
Mr. and Mrs. Robert M. Butcher
Mrs. Mary Ann Byers
Mr. Leroy G. Callahan
Cannon Design
Dr. and Mrs. Norman Chassin
Dr. and Mrs. David E. Chesebrough
Ms. Ann W. Christiansen
Mr. and Mrs. Frank L. Ciminelli
Dr. and Mrs. Abraham Clearfield
Miss Mary E. Clemesha
Mr. Donald H. Cloudsley
Mr. and Mrs. Daniel Cole
Mrs. Margaret R. Cole
Columbus McKinnon Corp.
Dr. and Mrs. Donald P. Copley
Mr. and Mrs. John Courtin
Mr. and Mrs. Robert B. Cozzens
Mrs. Robert C. Cummings
Mr. John T. Curtin
Dr. and Mrs. Donald P. Copley
Mr. and Mrs. John Courtin
Mr. and Mrs. Robert B. Cozzens
Mrs. Robert C. Cummings
Mr. John T. Curtin
Ms. Patricia De'Aeth
Mr. and Mrs. Anthony D. Decillis
Mr. & Mrs. Michael & Michele Degen
Mr. John Dicky
Mr. and Mrs. Joseph Downie
Mr. and Mrs. Kenneth Drake
Mr. John Dyster and Dr. Lyn Dyster
Mr. Wyndham Eaton
Mr. and Mrs. Frank Eberl
The Honorable & Mrs. John T. Elfvin
Mr. and Mrs. Richard L. Freeman
Mr. Richard L. Friend
Mr. John F. Fuchs
Mrs. Carolyn F. Gallivan
Mrs. Jean Gallmeyer
Mrs. Mona Gelbart
Ms. Lillian Gerstman
Dr. Debashis Ghosh
Mr. and Mrs. David W. Gow
Dr. Lee Ann Grace
Dr. Andrew Gulick, Ph.D.
Mr. and Mrs. Halem J. Habib
Mr. and Mrs. Calvin J. Haller
Mr. David G. Hanghauer
Mrs. Dorothy C. Hausle
Mr. and Mrs. Sherlock A. Herrick, Jr.
Hewlitt Packard Employee Charitable Giving
Program
Mr. and Mrs. Myron M. Hunt
HWI Staff
Dr. and Mrs. Theodore C. Jewett, Jr.
Johnson & Johnson
Mr. Edwin M. Johnston, Jr.
Dr. and Mrs. Kenneth R. Kahn
Mrs. Mary B. Kasbohm
Mr. Steven Kiss
Mrs. Betty D. Kittinger
Mr. John J. Klosterman
Dr. and Mrs. Todd B. Koch
Dr. Joseph Krasner
Mr. and Mrs. Charles F. Kreiner, Sr.
Mr. and Mrs. Robert J. Kresse
Mr. and Mrs. Ronald Krol
Mr. and Mrs. Timothy P. Lafferty
Mr. and Mrs. Sheldon T. Lenahan
Drs. David & Madeline Lillie
Mr. and Mrs. James A. Locke
Mr. John C. Lundrigan
Mr. and Mrs. Gary Maas
Mr. and Mrs. Robert J. Martin
Ms. Eileen R. McCallister
Mr. and Mrs. Kevin McDermott
Mrs. Barbara T. Meem
Militello's Luggage
Dr. and Mrs. Donald E. Miller
Mr. and Mrs. Glen Miller
Mr. and Mrs. John Mineo
Dr. Mahendra J. Mirani
Mrs. Peggy M. Moynihan
Mr. Edwin Munschauer, Jr.
Dr. and Mrs. Richard B. Narins
National Fuel Gas Company Foundation
Mr. and Mrs. Sanford M. Nobel
Dr. James Notaro
Parsons Brinckerhoff, Inc.
Mr. Kenneth R. Paslaqua
Mr. and Mrs. Richard S. Piccoli
Ms. Wendy Pierce
Mr. and Mrs. Michael A. Piette
Planned Futures Financial Group
Mr. and Mrs. H. William Pollack, II
Mr. and Mrs. John Edward Prise
Quaker Bonnet Eatery
Mrs. Lois F. Renz
Mr. and Mrs. George E. Riedel, Jr.
Robert D. Flickinger Foundation
Dr. Charlotte D. Roederer
Ms. Gladys Rosen
Mr. and Mrs. E. Peter Ruddy, Jr.
Mr. and Mrs. John R. Sanderson
Ms. Joan Schechtman
Mr. and Mrs. C. Jacob Schneider
Miss Irma Schultz
Mrs. Betty F. Seagrave
Mr. Norman C. Severo
Drs. Cathy Carter & James Shiffner
Mr. and Mrs. Francis Skop, Sr.
Mr. and Mrs. James R. Spengler, Jr.
Ms. Mary Ann Stiefel
Ms. Ruth V. Stockton
Ms. Doris E. Stone
Mr. and Mrs. Malcolm Strachan
Ms. Virginia D. Sullivan
Ms. Laurie A. Sutton
Dr. and Mrs. Thomas A. Szyperski
Dr. Hiroshi Takita
Mr. and Mrs. Franklin P. Taylor, Jr.
Mr. and Mrs. John H. Taylor
Ms. Janet Diana Vine
Mr. Louis Wagner
Dr. Charles M. Weeks
Mr. and Mrs. Edward C. Weeks
Ms. Gretchen White
Mrs. Paul A. Willax
Mr. and Mrs. William J. Williamson, Jr.
Mrs. Sarah B. Wilson
Ms. Sharon Winer
WNED-AM/FM
Mr. and Mrs. Frederick A. Wolf
FRIENDS (UP TO $99)
Mr. and Mrs. Gregory Abbott
Ms. Deborah Abgott
Mr. and Mrs. Morton Abramson
Aero Club of Buffalo
Mr. and Mrs. Thomas A. Alberalla
Mrs. Rosalyn Algase
Mrs. Jeanne Archer
Mr. Herbert A. Aurbach
Mr. and Mrs. Thomas P. Bagen
Mr. and Mrs. Gary R. Bainbridge
Dr. and Mrs. Thomas Bardos
Mr. and Mrs. Bruce Barit
Mr. and Mrs. Irving A. Barrett, Jr.
Mrs. Robert Barton
Mr. and Mrs. Martin Bates
Mr. and Mrs. Christopher R. Bechtel
Mr. and Mrs. Stephen R. Beck
Mr. Harry Beckerman
Mr. Ronald S. Benson
Dr. Victoria Besseghini
Mr. Jason P. Bray
Mrs. Pauline C. Breneman Noriega
Mr. and Mrs. Harvey J. Breverman
Mr. and Mrs. Dale Burrell
Mr. and Mrs. Richard Byron
Miss Mary Lou Cappellini
Mr. and Mrs. Lawrence S. Carr
Ms. Dorothea R. Carvalho
Mrs. John Cegielski
Mrs. Kathryn Cohen
Mr. and Mrs. Robert P. Coleman
Mr. Joseph A. Cozzarin
Mrs. Joseph D. Davis
Mrs. Grace R. de la Plante
Mr. and Mrs. Thomas Dearing
Gloria J. Del Bel
Mr. and Mrs. Edward L. Dentinger
Ms. Virginia Deuel
Miss Tinamarie DeVine-Zelasko
Mrs. Lynn F. Diviak
Ms. Helen Dobmeier
Mr. and Mrs. John B. Drenning
Mr. and Mrs. William A. Elliott
Mr. and Mrs. Maxwell Ellis
Mr. and Mrs. Richard C. Elsaesser
Mr. and Mrs. Aydin Erman
Ms. Marsha A. Fadale
Mr. and Mrs. Marwin L. Feldman
Mr. and Mrs. Jerrold S. Flaschner
Mr. and Mrs. David K. Floyd
Mr. and Mrs. Charles M. Fogel
Mrs. Helen T. Foley
Mr. and Mrs. Marvin Frankel
Ms. Candace A. Frerk
Mrs. Loise H. Frey
Dr. and Mrs. Eugene A. Friedberg
Mr. and Mrs. Arthur Fuchs
Mr. Ronald Galluzzi
Mr. and Mrs. Kenneth D. Garnjost
Genesee Valley Antique Car Society
Mr. and Mrs. Irwin E. Ginsberg
Dr. James E. Glogowski
Mr. Gerard J. Glowniak
Miss Audrey N. Golnick
Miss Ruth C. Graesser
Mr. and Mrs. Michael N. Grasso
Miss Rochelle A. Gray
Mrs. Clare Grellick
Mrs. Herman Haber
Mr. and Mrs. John A. Hahn
Mr. Paul A. Hahnel
Reverend and Mrs. James D. Hakes
Mary Elizabeth & David Handley
Mrs. Edythe T. Harris
Mr. and Mrs. Charles & Gloria E. Harter
Mr. and Mrs. Robert Hartney
Mr. and Mrs. Alden D. Harwood
Mr. and Mrs. Joseph J. Helfer
Mr. and Mrs. Charles T. Henrich
Dr. Mary Henrich Botsford
Mrs. William Reid Hensen
Mrs. Sheila M. Hess
Mr. and Mrs. Nelson Himmelfarb
Mr. and Mrs. Robert J. Hoag
Ms. Lorraine A. Hollister-Colby
Mr. and Mrs. James S. Howell
Dr. and Mrs. Edward Hyman
Mr. and Mrs. William G. Irr
Mr. and Mrs. Patrick R. Janiga
Mr. Daniel R. Jesser
Dr. and Mrs. Edward H. Jocoy
Joe Pollak & Sons, Inc.
Mr. and Mrs. Walter H. Johansson
Ms. Joan L. Josephson
Mrs. Ann E. Killian
Mr. and Mrs. Jacky Knopp Jr.
Mrs. Esther H. Koenig
Mrs. Margaret Kowalski
Ms. Laurie E. Krajna
Mr. and Mrs. Herbert P. Ladds, Jr.
Mr. Courtland R. LaVallee
Mrs. Kathleen A. LaVictor
Mrs. Aljean G. Leer
Dr. and Mrs. Harold J. Levy
Ms. Lenore Levy
Mr. Joseph R. Luft
Mr. and Mrs. David Macaluso
Mr. and Mrs. Stephen Manes
Dr. and Mrs. Paul V. Marrone
Mrs. Joanne Marzullo
Mrs. Santa M. Marzullo
Mr. Robert D. Maslanka
Dr. Irving J. Massey
Mrs. Sarah G. Metzger
Dr. and Mrs. Enrico Mihich
Mr. Zachary J. Miknis
Mrs. Darlene Miller
Mrs. Naomi K. Mintzer
Michael Timothy Moffit
Dr. and Mrs. Phillip Moudy
Mr. and Mrs. David R. Newcomb
Dr. John Notaro
Mr. and Mrs. James P. O'Brien
Ms. Emily D. Paolini
Mr. and Mrs. David M. Parish
Dr. Edwin V. Patricola
Mr. and Mrs. David & Cindy M. Pecynski
Mr. and Mrs. Richard M. Pirson
Mr. and Mrs. Harvey Podolsky
Mr. and Mrs. Edwin Presant
Senator Mary Lou Rath
Mrs. Edward L. Redmond
Mr. and Mrs. Richard G. Riebling
Mr. and Mrs. Robert S. Rochlin
Mrs. Dean M. Rockwell
Mr. and Mrs. Al Saia
Ms. Ida Schaer
Mr. and Mrs. Richard J. Schanley
Dr. and Mrs. Ray G. Schiferle
Mr. Walter R. Schoenfeld
Mr. and Mrs. Joseph G. Scully
Mr. and Mrs. Saunders M. Sennet
Mr. and Mrs. Gust D. Servis
Mr. Eugene M. Setel
Dr. Russell N. Shefrin
Mr. and Mrs. Walter S. Sikora
Ms. Mary B. Sippel
Mr. Abraham Slepian
Dr. and Mrs. Edward H. Snell
Mrs. Sylvia Sommerfield
Ms. Geraldine Sonnesso
Mr. and Mrs. Clarence E. Spitzer, Jr.
Mr. and Mrs. Gerald R. Stafford
Mr. and Mrs. Dean C. Stathacos
Mr. and Mrs. Edmund D. Stevens, III
Mr. and Mrs. Gerald R. Strauss
Ms. Karen A. Szalkowski
Mrs. Cecile Tegler
Mr. and Mrs. William Dennis Toole
Mrs. Constance S. Umland
Mrs. Patricia S. Utz
Valley View Nurseries, Inc.
Mr. and Mrs. John A. Vogel
Mrs. Laura M. Walfrand
Dr. and Mrs. James Warde
Mrs. Marie A. Weber
Mr. Raymond Weil
Ms. Marie L. Weisbecker
Dr. and Mrs. Robert M. Werner
Mr. and Mrs. Douglas L. Winokur
Mr. and Mrs. James Wise
Dr. and Dr. Thomas S. Wiswall
Mr. and Mrs. Marvin D. Wolfish
Mr. and Mrs. Frederick S. Wood
Mr. and Mrs. Martin S. Wright
Mr. and Mrs. Jerome Zelasko, Jr.
Mr. and Mrs. Harold A. Zimmer
Mr. and Mrs. Randal C. Zimmer
22 23

Recent Publications
• Cody, V., Luft, J.R. & Pangborn, W. (2005). Understanding the role of Leu22 variants in methotrexate resistance:
Comparison of wild-type and Leu22Arg variant mouse and human dihydrofolate reductase ternary crystal complexes
with methotrexate and NADPH, Acta Cryst. D61, 147-155.
• Cody, V., Chisum, K., Pope, C. & Queener, S.F. (2005). Purification and characterization of human-derived Pneumocystis
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24

The Hauptman-Woodward Medical Research Institute
Celebrating fifty years of basic research in the biosciences ...
dedicated to furthering science for the next fifty years and beyond ...
Select Awards and Honors In Recognition of Our New Building
The Hauptman-Woodward Medical Research Institute is celebrating 50 years
of groundbreaking research and beginning the next fifty years in our one-year
old home. Our new state-of-the-art facility gives us room to grow – growth that
will occur by recruiting new talent to Western New York. Our scientists are
striving to achieve greater scientific understanding and to educate the young
scientists of the future.
2005 Brick by Brick Award
Best Medical Complex
Architectural Engineering Award 2005
National Association of Industrial and Office Properties
The American Institute of Architects Buffalo
WNY Chapter, First Award
New Construction > 25,000 s.f.
President’s Message ……………………..………………………………......1
Chief Executive Officer’s Message …………………................................ 2
A Historical Perspective – 50 Years of Finding Cures ............................. 3
The Future – Forging Into The Next 50 Years …….………..……………... 9
The Science at the Hauptman-Woodward Medical Research Institute .... 13
The People of the Hauptman-Woodward Medical Research Institute ….. 18
Financial Summary ……………………………………………………….. 20
Donor Highlights ………………………………………………………..... 21