TROLL ® 9500 Water
Cable Connect 0085070
Direct Connect 0084310
RDO ® Optical Dissolved Oxygen Sensor
The In-Situ RDO ® optical dissolved oxygen sensor measures dissolved
oxygen (DO) using the principle of “dynamic luminescence quenching.”
Certain molecules, called “lumiphores,” fluoresce when excited by light of
a specific wavelength. Oxygen molecules act to quench this fluorescence.
The lumiphores in the sensor are embedded in a gas-permeable sensing
foil in a replaceable cap.
The sensor optics include a lens, blue LED and filter, red LED and filter,
and a photodetector. When the blue LED emits light, the sensing foil emits
red photons; the presence of oxygen in the foil causes a reduction in red
light detected by the photodiode. The phase difference between the blue
excitation light and the returned red light is measured, and the result is
used to compute DO.
This method measures the "phase" (or delay) of the returned signal, and is
thus based on the “lifetime” rather than the “intensity” of the luminescence.
Unpack the RDO Cable Connect Sensor
The RDO cable connect sensor arrives installed in a 3-part adapter/restrictor/
nose cone assembly with the sensor cap in a black canister. Remove the
black restrictor and nose cone from the sensor and adapter assembly.
The soft Cal Cup insert enables
calibration of the basic sensors
when the RDO sensor is installed.
It also contains an extra screw for the
Unpack the RDO Direct Connect Sensor
The RDO direct connect sensor ships in a box as shown below:
To use the RDO sensor, you will need the following items:
• TROLL 9500 instrument
• PC or RuggedReader handheld PC
• Most recent Win-Situ 4 or Pocket-Situ 4 software 1
• RDO sensor package
• Alkaline battery package (included with the TROLL 9500 instrument)
• Communication cable
• Most recent version of TROLL 9500 firmware
• TROLL Com cable
Installing the RDO Sensor Cap
1. Remove the red protective dust cap from the sensor and save it for later use.
Protective dust cap
2. Use the supplied lens wipe to clean the lens on the sensor, if necessary.
3. Remove the RDO sensor cap from its shipping sleeve.
4. Align the arrow on the cap with the index mark on the sensor and
firmly press the cap onto the sensor, without twisting, until it seals over
the probe body.
Keep the cap in its sealed packaging until you are ready to install it.
Make sure that the o-ring is not pinched or rolled inside the cap.
The cap's lifetime is 1 year after the first reading has been taken.
Install by the date printed on the packaging.
1 Make sure to use the most recent version of Win-Situ software. Free updates are available at
www.in-situ.com. You must have the most recent software version to allow firmware updates.
Installing the RDO Sensor
1. Install two D alkaline batteries in the TROLL 9500 instrument.
2. Remove the standard restrictor or Cal Cup from the TROLL 9500
instrument (if attached). This allows access to the sensor block.
3. Determine the installation port(s) for the RDO sensor. For the cable
connect, choose port 1 or 3. For the direct connect, you must use both
ports 2 and 3. Ports 1 and 4 will be available for other sensors.
The cap's lifetime is 1 year after the first reading has been taken
with the TROLL 9500 instrument. Install by the date printed on
Perform a 2-point calibration on the sensor, as described below.
Attach the flow restrictor and nose cone.
Use the Sensor Removal tool to remove the plug or existing sensor
from the appropriate port(s).
TIP: If installing the cable connector in port 3, you may wish to
remove the port 2 sensor temporarily (if installed).
Attach the RDO sensor to the TROLL 9500:
a. For the direct connect sensor, remove
the soft cap from the connector end of
the RDO sensor. Plug the sensor pins
into port 3. The sensor will overlap the
white port plug and the dummy will sit
in port 2. Proceed to step 6.
b. For the cable connect sensor, insert the front end of
the TROLL 9500 through the large hole in the adapter,
alongside the RDO sensor.
9. If using a cable connect sensor with a turbidity sensor or turbidity
wiper, pull the slack in the adapter cable up against the probe so that it
does not interfere with wiper movement or turbidity readings.
Initial 2-Point CALIBRATION
For best results, perform a 2-point calibration at 100% and 0% saturation
before going into the field.
1. Connect the TROLL 9500 instrument to a PC or RuggedReader
2. Launch Win-Situ 4 or Pocket-Situ 4; and "Find" the device. Select the
TROLL in the Navigation tree. The software will automatically detect
and display the installed sensors. (This can take a moment.)
3. In the Navigation tree, under Parameters, click on Rugged Dissolved
Oxygen. On the right side of the screen, click Calibrate.
c. Remove the soft cap from the connector end of the RDO
cable. Using the alignment notch, align the 3-pin connector
on the cable harness with the connector in the selected port
(1 or 3) of the TROLL 9500 and press it firmly into the port
until it sits flush with the instrument.
d. Press down on the adapter until the
instrument's sensor block is flush with the
adapter plate surface. Align the RDO
sensor between sensor ports 1 and 2.
Align the open hole for the RDO sensor
cable beside port 1.
e. Tighten the adapter screw with a Phillips screwdriver. Do
Remove the RDO sensor cap from its shipping/storage sleeve. Align
the arrow with the index mark and firmly press the cap onto the sensor,
without twisting, until it seals over the probe body. Make sure that the
o-rings are not pinched or rolled between the cap and the sensor.
Avoid allowing moisture, including atmospheric humidity, inside
the cap. Keep the cap in its sealed packaging until you are ready
to install it. Install promptly. Make sure that o-ring grooves are dry
and the o-ring is not rolled or pinched inside the cap.
Three options appear. To perform a calibration, select the first or
• Calibrate: Starting from values stored in the sensor (user-set or factory
defaults) for the 0% and 100% calibration, this allows the user to redo
one or both calibration points
• Restore defaults, then calibrate: Restores factory defaults for both 0%
and 100%, then allows the user to redo one or both calibration points.
• Restore defaults, don't calibrate: Restores factory defaults for both
0% and 100% saturation and closes the Calibration Wizard.
Indicators During Calibration
NOT TESTED is displayed until you begin the calibration by selecting Run.
UNSTABLE indicates the sensor response does not yet meet the criteria
for a valid calibration point.
NOMINAL indicates the sensor deviation meets early stabilization criteria.
The Accept button becomes available when nominal stability is achieved.
You may accept the early value or wait for complete stability. If you accept
the early value, the calibration point will be designated “USER SET” in the
STABLE is displayed when the readings have stabilized sufficiently to take
a valid calibration point. The calibration proceeds automatically to the next
• Reading: The current sensor response in mg/L and degrees Celsius.
• Deviation: Change in sensor response between the last two readings. This
enables you to follow the progress of the stabilization, but deviation from
the previous reading is not necessarily the best indicator of stability as the
software is looking at longer-term trends.
• Barometric Pressure is shown in your selected unit.
Preparation for 100% Oxygen Calibration
100% DO calibration is performed in water saturated with air. We
recommend a container of clean water aerated with the In-Situ RDO
bubbler cal kit (see Replacement Parts list) or an aquarium pump.
Fill the bubbler cup with clean water. Turn on the pump and regulate
with the pinch valve. Insert the TROLL 9500 instrument into the bubbler,
resting the RDO adapter plate on the rim of the cup. Ensure that the
temperature sensor is submerged at least ½ in, and the RDO sensing
foil is not in the direct path of the bubbles.
5. After allowing 5 – 10 minutes for temperature stabilization, click the
Run button. Follow the on-screen instructions to complete the 100%
6. Continue with the 0% Oxygen Calibration for a 2-point calibration or
select Next, then Finish to finalize the calibration.
Preparation for 0% Oxygen Calibration
0% D.O. calibration is performed in an oxygen-depleted solution. Sodium
sulfite (Na 2
, see Replacement Parts list) is available from In-Situ Inc.
Bubbled nitrogen may be used; in this case allow plenty of time for the
oxygen to be completely purged from the water.
Units and Calculated Measurements
Four units of measurement are available for DO:
TIP: To change unit preferences: In Win-Situ 4, select
Preferences on the Options menu. In Pocket-Situ, select the
Home site, then tap Setup in the command bar.
• Oxygen concentration in milligrams of oxygen per liter of water (mg/L).
Since a liter of pure water weighs 1000 grams, and a milligram is
1/1000 of a gram, this is equivalent to ppm (parts per million).
• Oxygen concentration in micrograms of oxygen per liter of water (µg/L). This
is equivalent to parts per billion (ppb).
• Oxygen concentration in microMolar (µmol/L) = mg/L x 31.25
• Oxygen saturation in % —100% DO being the maximum (equilibrium)
amount of oxygen that can be dissolved in water at a given atmospheric
pressure, water temperature, and salinity. The percent saturation
output is automatically corrected using the TROLL 9500 instrument's
temperature, conductivity, and barometric pressure values (from a
barometric sensor on vented cable or from a user-entered input). If no
conductivity sensor is present, salinity is assumed to be zero.
USAGE RECOMMENDATIONS AND CAUTIONS
The amount of oxygen that can be dissolved in water decreases at higher
temperatures and with increasing altitude (i.e., as barometric pressure
drops) and salinity. In other words, as water becomes warmer and saltier, it
can hold less DO.
During tests that include the RDO channel, the TROLL 9500 instrument
automatically measures barometric pressure and temperature for
compensation of DO readings.
The barometric pressure value at the time of calibration is stored in the
sensor and will be used to correct DO readings for weather-induced
pressure fluctuations taken at the same relative barometric pressure
as the calibration. However, the large changes in barometric pressure
due to changes in elevation are best accommodated by performing a
recalibration. If you calibrate at sea level, for example, and deploy the
instrument in the mountains, you should perform a recalibration at the
Exposure to direct sunlight can bleach the sensing foil over time but this
effect is minimized with the protection provided by the opaque optical
isolation layer. However, the frequent application of strong blue light (blue
LED during readings) will eventually have a bleaching effect on the foil.
In-Situ recommends sampling intervals of greater than 1 minute for longterm
Warning: The RDO sensor is not compatible with organic
Since the absolute solubility of oxygen is lower in saline waters, it is
advantageous to compensate DO concentration readings (µg/L, mg/L,
µmol/L) to ensure that the sensor accurately reports the concentration of
DO in the presence of significant salinity. This can be accomplished by
storing a salinity value in the sensor before taking measurements. The
compensation algorithm is applied internally before the concentration is
reported. The degree of compensation is minimal at very low salinities, and
but may affect readings by several percent at higher levels.
To store a salinity value in the RDO sensor, connect in Win-Situ 4 or
Pocket-Situ 4, select RDO in the Navigation tree, click Edit and in the next
screen select "Edit RDO Salinity Value."
Maintenance AND STORAGE
Cleaning the Sensor Cap
Leave the cap on the sensor!
Rinse the sensor with clean water from a squirt bottle or spray bottle.
Gently wipe with a soft cloth or brush if biofouling is present.
If extensive fouling or mineral build-up is present, soak the cap end in
vinegar for 15 minutes, then soak in deionized water for 15 minutes.
Do not use organic solvents—they will damage the foil. Do not
remove the cap from the sensor prior to brushing.
Usage Life of Cap
Shelf Life of Cap
Storage Conditions, Cap
After cleaning the sensor, perform a 2-point user calibration.
Cleaning the Optical Window (Perform only when changing the cap.)
Remove the cap and gently wipe the window with the supplied lens wipe.
Caution: Do not wet the lens area with water or any solution.
Cleaning the Sensor Body
With the sensor cap installed on sensor, gently scrub sensor body with a
soft brush. Soak in vinegar and DI water to remove mineral deposits or
extensive fouling as in step 4, above.
Storage Conditions, Sensor
ASTM D888-05 Test Method C
0 to 20 mg/L concentration,
0 to 450% saturation
±0.1 mg/L, 0 to 8 mg/L
±0.2 mg/L, 8 to 20 mg/L
T90: 30 sec T95: 37 sec
1 year from the first instrument reading
24 months from date of manufacture
0° to 50°C (32° to 122°F)
300 PSI (210 m, 692 ft)
1° to 60°C (33° to 140°F), in factorysupplied
-5° to 60°C (23° to 140°F)
Up to 42 ppt
Prior to installation: Store in factory supplied container
Installed: Store as per requirements of other installed sensors, for
example, pH, if installed. Keep or store in a moist environment, such as
the recyclable calibration and storage sleeve or the optional calibration and
Insert the 9500 instrument with
attached RDO sensor into its Cal cup
For the sub-4 in model, attach
the red plug to seal the top of the
Alternatively, place a sleeve inside
restrictor. Add a small amount of
water or pH buffer to keep moist.
Replacing the Sensor Cap
The sensor cap has a 1-year life after the TROLL 9500 instrument takes
its first RDO sensor reading. Replacement caps are available from In-Situ
Inc. or your authorized In-Situ distributor.
1. Pull the used sensor cap off of the sensor, without twisting.
2. Remove the existing o-rings from the sensor.
3. Use a lint-free cloth to remove any moisture from the sensor body.
Note: Ensure that there is no moisture in the o-ring grooves.
Avoid touching or cleaning the lens with anything other than the
supplied lens wipe.
4. Use your finger to apply a layer of lubricant around the o-ring grooves.
Place the o-rings on the sensor. Apply another thin layer of lubricant to
the o-rings and grooves
Note: Do not transfer grease to the lens or sensor pins.
5. Clean the lens on the sensor with the wipe provided in the kit and allow
to dry thoroughly. Inspect for scratches or dirt.
6. Remove the new cap from its sealed packaging and attach it to the
sensor, being careful to press firmly, without twisting, until it seals over
the probe body. Make sure that the o-rings are not pinched or rolled
between the cap and sensor.
7. Perform a 2-point calibration as described in the RDO Instruction
Sheet or TROLL 9500 Operator's Manual.
RDO Sensor Cap Replacement Kit............................................................................................................................................................................... 0084230
0% Calibration Solution (Sodium sulfite, Na 2
), 500 mL........................................................................................................................................... 0017670
0% Calibration Kit (pint Sodium sulfite, quart DI water).................................................................................................................................................0032110
RDO Calibration Kit for Cable Connect RDO sensor (Bubbler), includes 500 mL sodium sulfite................................................................................. 0048580
RDO Calibration Kit for Direct Connect RDO sensor (Bubbler), includes 500 mL sodium sulfite................................................................................. 0080830
Calibration and Storage Sleeves, recyclable, (100/pkg).............................................................................................................................................. 0060870
1 800 446 7488
(toll-free, US and Canada) or 970 498 1500
Information is subject to change without notice. In-Situ and the In-Situ logo, Win-Situ, TROLL, BaroTROLL, RuggedReader, RuggedCable, and RDO are
trademarks or registered trademarks of In-Situ Inc. Copyright © 2008-2009 by In-Situ Inc. All rights reserved.
rev 005 0085072 01/09