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iXon Ultra Opto Bundle (PDF)

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Andor’s Supercharged <strong>iXon</strong> <strong>Ultra</strong> EMCCD<br />

Accelerated!<br />

New<br />

NOW AVAILABLE WITH<br />

OPTICALLY CENTRED CROP MODE<br />

ENABLING<br />

LIVE CELL SUPER-RESOLUTION<br />

MICROSCOPY<br />

<strong>iXon</strong> <strong>Ultra</strong> EMCCD<br />

and <strong>Opto</strong>Mask<br />

Achieve 569 fps from a 128 x 128 centralized ROI<br />

Limited time offer ...<br />

Purchase:<br />

• <strong>iXon</strong> <strong>Ultra</strong> 512x512 back-illuminated EMCCD camera<br />

• Receive a FREE <strong>Opto</strong>Mask<br />

<strong>Bundle</strong><br />

Offer<br />

Andor’s new ‘supercharged’ <strong>iXon</strong> <strong>Ultra</strong> 897 back-illuminated<br />

EMCCD is now overclocked to 17 MHz, performing more than<br />

60% faster.<br />

Combine with the <strong>Opto</strong>Mask accessory to operate in Crop<br />

Mode, boosting Region of Interest acquisition to unparalleled<br />

speeds, for example achieving 569 fps from a 128 x 128 ROI<br />

(optically centred on the sensor).<br />

Applications:<br />

• Single Molecule<br />

• Super-resolution (inc. Live cell)<br />

• Calcium / Ion signalling<br />

• Vesicle trafficking<br />

• Voltage sensitive dyes<br />

• Cell Motility


Crop Mode Explained<br />

In Crop Mode, the user defines a ‘Region of Interest’ that encompasses the part of the image where change<br />

is rapidly occurring, e.g. a ‘calcium spark’ within a cell. The sensor subsequently “imagines” that it is of this<br />

smaller defined array size, achieved through special readout patterns, and reads out at a proportionally faster<br />

frame rate. The smaller the defined array size, the faster the frame rate achievable.<br />

Since in Crop Mode pixels outside the ROI are effectively ignored during readout, one has to ensure that no<br />

light is falling on the light sensitive area outside of the defined region. Any light collected outside the cropped<br />

area could corrupt the images which were acquired in this mode. This is now facilitated using the <strong>Opto</strong>Mask<br />

accessory, which can be flexibly adjusted to match whatever ROI size is required to frame the observed<br />

section. In contrast, a ‘conventional’ ROI readout carries the significant overhead of having to shift and<br />

discard unwanted pixels. As such, Crop Mode has the end result of achieving a much faster frame rate than<br />

that obtainable in a conventional ROI, but can still be centrally located within the sensor.<br />

Head Office<br />

7 Millennium Way<br />

Springvale Business Park<br />

Belfast BT12 7AL<br />

Northern Ireland<br />

Tel: +44 (28) 9023 7126<br />

Fax: +44 (28) 9031 0792<br />

North America<br />

425 Sullivan Avenue<br />

Suite 3<br />

South Windsor, CT 06074<br />

USA<br />

Tel: +1 (860) 290 9211<br />

Fax: +1 (860) 290 9566<br />

<strong>iXon</strong> <strong>Ultra</strong> EMCCD<br />

• Single Photon Sensitive<br />

• Back-illuminated 512 x 512 sensor<br />

• > 90% QE<br />

• 56 fps full frame<br />

• 595 fps from 128 x 128 (cropped sensor)<br />

• -100°C cooling<br />

• USB 2.0<br />

<strong>Opto</strong>Mask<br />

• Flexible optical masking of sensor<br />

• 4x adjustable blades<br />

• Fits easily onto microscope C-mount<br />

• Designed for cropped sensor mode<br />

Frame rates available from <strong>iXon</strong> <strong>Ultra</strong> 897 in cropped sensor mode<br />

‘Optically Centred’ ROI speeds shown in brackets.<br />

Array Size<br />

Binning 256 x 256 128 x 128 64 x 64 32 x 32 512 x 100 512 x 32<br />

1 x 1<br />

2 x 2<br />

4 x 4<br />

111<br />

(174)<br />

215<br />

(329)<br />

402<br />

(594)<br />

595<br />

(569)<br />

1,085<br />

(1,014)<br />

1,802<br />

(1,662)<br />

1,433<br />

(1,492)<br />

2,433<br />

(2,329)<br />

3,577<br />

(3,237)<br />

3,532<br />

(3,024)<br />

5,325<br />

(4,054)<br />

6,579<br />

(5,252)<br />

296 857<br />

570 1,589<br />

1,050 2,682<br />

Japan<br />

4F NE Sarugakucho Building<br />

2-7-6 Sarugaku-Cho<br />

Chiyoda-Ku<br />

Tokyo 101-0064<br />

Japan<br />

Tel: +81 (3) 3518 6488<br />

Fax: +81 (3) 3518 6489<br />

China<br />

Room1213, Building B<br />

Luo Ke Time Square<br />

No. 103 Huizhongli<br />

Chaoyang District<br />

Beijing 100101<br />

China<br />

Tel: +86 (10) 5129 4977<br />

Fax: +86 (10) 6445 5401<br />

Request a quote now: andor.com/ultraoptobundle<br />

Offer ends 30 th September 2013<br />

Crop Mode is recommended with the following software options:<br />

• Andor Solis*<br />

• Andor SDK*<br />

• Metamorph<br />

• NIS Elements*<br />

• Image Pro<br />

• µManager*<br />

• LabVIEW*<br />

• MatLab*<br />

* Compatible now with new ‘Optically Centred Crop Mode’.

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