Bacteria from Fish and Other Aquatic Animals - Survival-training.info

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vi Contents 5.2 Microscopy 224 5.3 Storage of Isolates 224 6 Techniques for the Molecular Identification of Bacteria 225 6.1 Molecular Identification by PCR Using Specific Primers 225 6.2 PCR Protocols 234 6.3 Molecular Identification by 16S rDNA Sequencing 236 6.4 Fluorescence in Situ Hybridization (FISH) 242 7 Preparation of Media for Culture and Identification 244 7.1 General Isolation and Selective Media 244 7.2 Biochemical Test Media 261 Further Reading and Other Information Sources 278 Appendix: Common Name and Scientific Name of Aquatic Animals 281 Glossary of Terms 287 References 292 Index 329
List of Tables and Figures Tables Table 1.1. Table 1.2. Table 2.1. Table 2.2. Table 2.3. Table 2.4. Table 3.1. Table 3.2. Table 3.3. Table 3.4. Table 3.5. Table 3.6. Table 3.7. Table 4.1. Table 4.2. Table 4.3. Table 4.4. Table 4.5. Table 4.6. Table 4.7. Table 4.8. Table 4.9. Table 4.10. Table 4.11. Table 4.12. Table 4.13. Table 4.14. Table 4.15. Table 4.16. Table 4.17. Table 4.18. Host species and organism relationship. Bacterial pathogens and saprophytes of fish and other aquatic animals. Outline of steps for culture and identification. General culture. Specific culture requirements of organisms. Microscopic and cultural characteristics. Interpretation of tests for ‘biochem set’. Carnobacterium, Lactobacillus, Vagococcus and Renibacterium differentiation. Differential tests for some non-fastidious fish pathogenic Gram-positive cocci and rods. Differential characteristics of the genera within the family Flavobacteriaceae. Further differential characteristics for the genera in the family Flavobacteriaceae. Additional tests for differentiation of biotypes of P. damselae. Differentiation of V. alginolyticus and V. harveyi. Aeromonas salmonicida (non-motile Aeromonas species). Aeromonas spp. Phenotypic tests according to DNA hydridization groups. Aeromonas spp. – motile. Anaerobes. Brucella spp. Cryptococcus. Cytophaga–Flavobacteriaceae–Bacteroides group – pathogenic. Cytophaga–Flavobacteriaceae–Bacteroides group – environmental. Gram-negative coccobacilli and cocci. Gram-negative, oxidase-negative rods. Gram-negative, oxidase-positive rods. Helicobacter spp. Gram-positive cocci. Gram-positive rods. Mycobacterium and Nocardia spp. Mycobacterium spp. – additional tests. Mycoplasma spp. Listonella spp. vii
Page 1 and 2: Bacteria from Fish and Other Aquati
Page 3: Contents List of Tables and Figures
Page 7 and 8: Foreword While aquatic animal disea
Page 9 and 10: Acknowledgements xi Principal spons
Page 11 and 12: Introduction xiii Medical laborator
Page 13 and 14: Photographs of Culture and Microsco
Page 15 and 16: Fig. 11. Aeromonas veronii ssp. sob
Page 17 and 18: Fig. 22. Edwardsiella tarda on BA,
Page 19 and 20: Fig. 43. Listonella anguillarum, Gr
Page 21 and 22: Fig. 55. Plesiomonas shigelloides,
Page 23 and 24: Fig. 69. Vibrio alginolyticus, Gram
Page 25 and 26: Fig. 82. Vibrio harveyi on MSA-B, 4
Page 27 and 28: Fig. 98. Vibrio proteolyticus on BA
Page 29 and 30: Fig. 118. Methyl Red reaction. Posi
Page 31 and 32: 2 Chapter 1 Table 1.1. Host species
Page 33 and 34: 4 Chapter 1 Table 1.1. Continued. H
Page 39 and 40: 10 Chapter 1 Table 1.1. Continued.
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26 Chapter 1 Table 1.1. Continued.
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36 Chapter 1 Bacterial gill disease
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38 Chapter 1 Table 1.2. Bacterial p
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76 Chapter 1 of the test, or the us
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78 Chapter 1 in a similar manner to
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80 Chapter 1 phenotypic results rec
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82 Chapter 1 formerly Y. enterocoli
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84 Chapter 2 2.1 Specimen Collectio
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86 Chapter 2 Table 2.3. Specific cu
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114 Chapter 2 reaction, oxidase and
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116 Chapter 2 API 20E API 20NE API
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118 Chapter 3 Table 3.1. Interpreta
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120 Chapter 3 to produce alkaline p
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122 Chapter 3 V. splendidus biovar
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124 Chapter 3 are likely to produce
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126 Chapter 3 Table 3.2. Carnobacte
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128 Chapter 3 reported a negative r
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130 Chapter 3 Table 3.5. Further di
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132 Chapter 3 to be due to the diff
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134 Chapter 3 control and a negativ
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136 Chapter 3 Yersinia ruckeri Stra
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138 Chapter 4 Fig. 4.1. Schematic f
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140 Table 4.1. Aeromonas salmonicid
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142 Table 4.3. Aeromonas spp. - mot
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144 Table 4.5. Brucella spp. Test G
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146 Table 4.8. Cytophaga-Flavobacte
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148 Table 4.8. Continued. Test Gm O
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150 Table 4.10. Continued. Test Gm
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160 Table 4.13. Gram-positive cocci
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164 Table 4.14. Gram-positive rods.
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166 Table 4.15. Mycobacterium and N
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168 Table 4.17. Mycoplasma spp. Tes
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178 Table 4.23. API 20E database bi
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180 Table 4.23. Continued. Tests on
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182 Table 4.23. Continued. Tests on
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184 Table 4.24. API 20E database nu
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186 Table 4.24. Continued. Organism
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190 Table 4.25. API 20E database nu
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192 Table 4.25. Continued. API 20 E
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198 Table 4.26. Continued. Listonel
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200 Table 4.27. API 50CH database b
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202 Table 4.27. Continued. 1 2 3 4
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208 Table 4.28. API Coryne database
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210 Table 4.30. API Rapid ID32 Stre
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212 Table 4.31. API ZYM database re
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214 Table 4.31. Continued. Well 1 2
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216 Table 4.31. Continued. Well 1 2
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218 Table 4.32. Strains quoted in r
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5 Technical Methods 5.1 Total Bacte
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224 Chapter 5 Incubation Place plat
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226 Chapter 6 Table 6.1. List of sp
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232 Chapter 6 Edwardsiella spp. PCR
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234 Chapter 6 Extraction of DNA fro
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236 Chapter 6 The nucleotides can a
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238 Chapter 6 Table 6.3. Universal
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240 Chapter 6 Visualize DNA on agar
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242 Chapter 6 document with the seq
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7 Preparation of Media for Culture
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246 Chapter 7 Blood agar - BA Reage
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248 Chapter 7 Edwardsiella ictaluri
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250 Chapter 7 Flexibacter maintenan
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252 Chapter 7 Marine 2216 agar (Dif
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254 Chapter 7 Mycoplasma medium - m
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256 Chapter 7 R2A agar (Oxoid CM 90
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258 Chapter 7 Siem selective medium
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260 Chapter 7 VVM Selective medium
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262 Chapter 7 Brucella: growth on s
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264 Chapter 7 Carbohydrate fermenta
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266 Chapter 7 Congo Red. Tests for
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268 Chapter 7 Furunculosis agar (Fo
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270 Chapter 7 Indoxyl acetate hydro
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272 Chapter 7 Glucose (10% w/v) 10
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274 Chapter 7 ONPG - o-nitrophenyl-
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276 Chapter 7 Salt - 20% stock solu
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Further Reading and Other Informati
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280 Further Reading List of Bacteri
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282 Appendix Common name Scientific
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288 Glossary C Ca Cat CBBA CCA CCRC
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290 Glossary O Oxidative (aerobic m
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References 1 Abbott, S.L., Cheung,
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294 References otherwise infected w
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296 References 87 Bergman, S., Seli
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298 References Journal of Systemati
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300 References ribonucleic acid seq
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302 References ‘winter disease’
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304 References 273 Fukuda, Y., Mats
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306 References resistance of virule
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308 References and an emended descr
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310 References the Enterobacteriace
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312 References an epizootic of cult
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314 References 512 Macián, M.C., L
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316 References Plecoglossus altivel
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318 References sponge. Internationa
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320 References fish pathogens. Dise
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322 References Listonella and reass
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324 References 750 Toranzo, A.E., B
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326 References 795 Vreeland, R.H.,
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328 References 842 Wood, R. (1965)
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330 Index Aeromonas continued A. ca
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332 Index API identification system
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334 Index Brucella agar 246 Brucell
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336 Index Colony forming units (CFU
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338 Index Dolphin continued Striped
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340 Index Flavobacterium species co
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342 Index Granulicatella continued
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344 Index Laminaria 59 Laminaria ja
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346 Index Mycobacterium continued M
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348 Index Peled see Whitefish Pelec
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350 Index Pseudoalteromonas continu
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352 Index Salmonella continued S. j
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354 Index Shewanella marine agar (S
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356 Index Strontium chloride B enri
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358 Index Vibrio continued V. brasi
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360 Index Vibrio continued V. scoph
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