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30 fusion of Brassica carinata BC1 x Brassica rapa subsp. oleifera f. praecox 31/96. Preliminary flow cytometry analyses showed a high rate of somatic hybrids (85%). Further morphological and cytological analyses will be performed after the subcultivation of regenerants to greenhouse conditions. (Vyvadilová M., Klíma M.) Sources of resistance and innovation of selection methods for breeding for multiplex resistance to the main wheat and oat leaf diseases (MZe âR 1G58083) The topic was investigated in cooperation with the research centre SELTON. Resistance of wheat to the following pathogens: Puccinia triticina, P. striiformis, P. graminis, Blumeria graminis, Pyrenophora tritici-repentis, Mycosphaerella graminicola and resistance of oats to Puccinia coronata f.sp. avenae were studied. Ring tests with single pathogens were carried out at several locations. These tests enabled to determine multiplex resistance. Resistance was also studied in greenhouse tests on seedlings and on detached leaves. In these tests resistance to rusts, Pyrenophora tritici-repentis and Mycosphaerella graminicola was studied. Pathotypes of wheat leaf rust and oat crown rust, as well as of Mycosphaerella graminicola were studied and total virulence of powdery mildew was determined on a set of differentials. The comparison of the greenhouse and field tests enabled to distinguish resistance effective during the whole ontogenesis from the adult plant resistance. According to reactions of cultivars to a set of selected pathotypes the translocation 1BL.1RS possessing resistance genes Lr26, Sr31,Yr9 and Pm8 as well as the translocation from Aegilops ventricosa carrying resistance genes Lr 37, Sr38, Yr17 were postulated in several registered winter wheat cultivars. (Hanzalová A., Barto‰ P., Dumalasová V.,Vûchet L., Klenová H.) Genetic, physiologic and molecular analysis of a novel flowering time gene in wheat (GA âR 521/05/0257) The development of plants of spring cultivar Zlatka and of its substitution line Zlatka (âP3B) grown in the short day (10 hours photoperiod, RICP shaded field plots) was checked repeatedly to complete information on the earliest stages where different dynamics of main spike differentiation due to the chromosome 3B substitution had been observed. SSR molecular fingerprinting of the Zlatka (Zlatka 3B/ CP 3B) single chromosome recombinant F3 lines was carried out to develop a further comprehensive map of the chromosome 3B. The acquired data were processed using computer software available at JIC to develop a new genetic map of chromosome 3B and to compare it with the previous map developed using the Sandra (Sandra 3B/CP 3B) single chromosome recombinant population map, and other 3B maps available at the JIC based on the recombinant doubled haploid populations of UK winter wheats Spark x Rialto and Charger x Badger.The genetic maps were all co-linear and indicated that the genetic maps available of the CP 3B chromosomes should provide sufficient coverage to map the genes for flowering time and other traits.Two markers were common between the Zlatka /CP 3B and Sandra /CP 3B, which should also enable the aligning of QTL data – see Figure. (Pánková K., Prá‰il I., DoleÏel J., Snape J. W.) Advanced techniques in obtaining and processing crops research results (Leonardo, the project of mobility, CZ/06/A/PL/134148) The substitution and recombinant substitution lines which were the focus of the genetical analysis experiments carried out by the trainee were derived from a range of chromosome substitution lines developed in RICP, Ruzynû, Prague. The first task was to monitor the validity of historic collections maintained in RICP through the use of molecular markers.The second task was to carry out a large experiment to study variation in the flowering time of F3 recombinant lines derived from Sandra (CP 3B).An experiment under controlled conditions was established in a controlled environment room, and phenotypic analysis of flowering time and other characters was carried out. Additionally, samples were collected which were used for DNA extraction to develop and check the genetic map of the 3B chromosome. The genetic map and phenotypic data were combined to carry out QTL analysis to locate quantitative trait loci for flowering time and other traits. (Pánková K., Milec Z., Snape J. W.) Comparative maps of wheat chromosome 3B
31 STAFF OF THE DIVISION Ing. Ladislav Dotlaãil, CSc. (Head of the Division) Jitka Hourová (Personal Secretary) DEPARTMENT OF GENE BANK Ing. Zdenûk Stehno, CSc. (Head of the Dept.) Milena Aulická Ing. Jan Bém Jaroslava Dotlaãilová Ing.Václav Dvofiáãek, Ph.D. Mgr. Iva Faberová Ing. Jifií Hermuth Ing.Vojtûch Holubec, CSc. Ing. Dagmar Janovská Milan Ko‰afi Michaela Martínková Marie Marxová Jarmila Pa‰ková Ing. Eva Pelichová Alena Po‰tová, Bc. Martin Procházka Jana Ráli‰ová Petra Zábranská Emanuela Zabuìková Workplace Olomouc Ing. K. Du‰ek, CSc. (Head of the workplace) Anna Calábková Ing. Elena Du‰ková Libu‰e Golová Ing.Vûra Chytilová Ing. Katefiina Karlová, Ph.D. Drahomíra Kocmánková Vlasta Koãí Ing. J. Losík Jan Militk˘ Vlasta Schubertová Vûra Sklenáfiová Ing. Helena Stavûlíková, Ph.D. Jarmila Vyhnánková DEPARTMENT OF PLANT BREEDING METHODS Ing.Václav ·íp, CSc. (Head of the Dept.) ·árka Bártová Marie Berová Ing. Ladislav Bláha, CSc. Ing. Jana Bradová Mgr. Lucie Davidová J. Dvofiák Ing. Jana Chrpová, CSc. RNDr. Eva Matûjová Jan Matou‰ek Ing. Ludmila Papou‰ková RNDr. Ilja Prá‰il, CSc. Ing. Pavla Prá‰ilová Mgr.TaÈána Sumíková Mgr. Svûtlana S˘korová, CSc. Mgr. Pavel Vítámvás Marie Vlãková DEPARTMENT OF MOLECULAR BIOLOGY RNDr. Jaroslava Ovesná,CSc. RNDr. Alois Bilavãík, Ph.D. Ing. Milo‰ Faltus, Ph.D. Markéta Hanu‰ová Mgr. Jan Hodek Mgr. David Cháb Bc. Petra Koucká Ing. L. Kuãera, CSc. Ing. Dagmar Kuãerová Mgr. Leona Lei‰ová Bc. Michal Mal˘ Mgr. Lucie Pavlátová Katefiina Pe‰ková Ing.Vladimíra Pouchová Mgr. Jana Rulcová Ing. Blanka Svejkovská Ing. Kamila ·tastná Ing. Lenka Tomková Mgr. Iva Tom‰íková Hana Udavská Mgr. Petra Wildová Ing. Jifií Zámeãník, CSc. (Head of the Dept.) DEPARTMENT OF APPLIED GENETICS Ing.Vratislav Kuãera, CSc. (Head of the Dept.) Ing. Pavel Barto‰, DrSc. Bc. Petra Barto‰ová RNDr.Veronika Dumalasová, Ph.D. Martina Fajferová SoÀa Fajmanová Mgr. Alena Hanzalová Ing. Miroslav Klíma Jitka Hanzlíková Ing. Zdenûk Milec Radoslava Ondra‰íková Mgr. Katefiina Pánková Jaroslav ·rámek Václava Stfiesková Ing. Lubomír Vûchet, CSc. Ing. Miroslava Vyvadilová, CSc.
Page 1 and 2: 13 DIVISION OF GENETICS AND PLANT B
Page 3 and 4: 15 of all available generatively pr
Page 5 and 6: 17 Plant biodiversity conservation
Page 7 and 8: 19 Fig. 2. Genetic distances within
Page 9 and 10: 21 the 6 th Framework Programme. 11
Page 11 and 12: 23 concentrations of DON were detec
Page 13 and 14: 25 cial seed of wheat, barley and p
Page 15 and 16: 27 a preculture-desiccation method
Page 17: 29 cytological checks of the aneupl

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