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Dr. Joseph Irudayaraj - Center for Food Safety Engineering - Purdue ...

Dr. Joseph Irudayaraj - Center for Food Safety Engineering - Purdue ...

Dr. Joseph Irudayaraj - Center for Food Safety Engineering - Purdue

11/24/2009 ARS-USDA and Purdue Center for Food Safety Engineering July 2007 – March 2009 Biosensor Integrated Spectroscopy for Pathogen Capture &D Detection ti PIs: Irudayaraj, J.; Mauer, L.; and * Debroy, C. Purdue University; * Penn State University Objectives • Develop fingerprint library using Raman and FTIR for key foodborne strains Investigate microbial components Dead vs live; Treatment conditions • Advance portable infrared biosensor Optimize biosensor platform Appropriate sampling methods and Testing Nanoscale biosensors Pathogens tested • E.coli E. coli O26, E. coli O103, E. coli O111, E. coli O157:H16, E. coli 0157:H5, E. coli O157:H19 . E. coli O157:H7 (Acc No: 5.2262, 99.0874, 0.1292, 99.0894, 0.0027, 0.1288, 0.1304, 7.3853, 7.3860) • Salmonella S. typhimurium, S. enteritidis • Listeria L. innocua, L. monocytogenes • Shigella flexneri, Staphylococcus aureus FTIR vs Raman Absorban nce (arbitrary unit) 1.2 1 0.8 0.6 0.4 0.2 0 Escherichia Coli O157:H7 Raman 400 600 800 1000 1200 1400 1600 1800 Wavenumber (cm -1 ) Raman spectrum (red) is more highly resolved than the FTIR spectrum (purple). FTIR Raman and FTIR discrimination Raman FTIR Strain level discrimination by Raman and FTIR Raman FTIR Differentiation of five different species of pathogenic bacteria based on the canonical variates Discrimination of five different E.coli O157:H7 strains obtained from different sources. 1

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