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Preface - kmutt

Preface - kmutt

KMUTT Annual Research

KMUTT Annual Research Abstracts 2005 Suthitar Singkaravanich, Taweerat Vichitsoonthonkul The 1 st International Conference on Fermentation Technology for Value Added Agricultural Products, March 22-25, 2005, Kosa Hotel, Khon Kaen, Thailand, p. 83 The effect of mechanical shear forces on growth and enzyme production of Aspergillus oryzae in solid state fermentation (SSF) using rotating drum bioreactor (RDB) was studied. The fermentation had been carried out in the stationary conditions on opened and closed plates and in a 22.7 litre horizontal rotating drum bioreactor with continuous mixing at three levels: 5, 9 and 20 rpm. The mixed substrates of cassava pulps and defatted soy bean residues were used throughout the study. The results revealed that the highest biomass and amylase activity were obtained in the opened plate culture. Whereas the highest protease activity was found in closed plates. Agitation in RDB dramatically reduced growth and enzyme production. The fermentation at 9 rpm could encourage growth and enzyme production when compared with those from other agitation speeds. The intracellular protein patterns were determined to study the cellular response to the stress of mixing in RDB. The SDS-PAGE analysis identified a possible stress response protein with the approximate size of 20 Kda. This protein was found intensively in cell grown at high speed (i.e. 9 and 20 rpm) fermentation. IC-239 SOFTWARE SENSOR FOR MONITORING OF BIOREACTOR Anan Tongta The 1 st International Conference on Fermentation Technology for Value Added Agricultural Products, March 22-25, 2005, Kosa Hotel, Khon Kaen, Thailand, p. 39 Consistency and reproducibility are important requirements for biological production processes. To achieve these requirements, reliable monitoring and control system has to be developed. Understanding of the insight of the processes is a goal for a successful monitoring and control system. The consistency of the process is an important part of the current good manufacturing practice (cGMP). The introduction of computerized measurement and control has made it possible to apply on-line modeling and optimization of the bioprocess. 159 The yield and the consistency of the process could be improved with the advent of the process automation. The classical monitoring and control methods do not prove to be efficient enough for the biological processes due to the complexity of the bioprocess dynamics. The efficiency of the monitoring and control systems will be improved if they are able to incorporate the important information on the process and deal with the missing information (due to the lack of on-line measurements, uncertainty with regard to the dynamics, etc.). In this study, model based software sensors for on-line estimations of biological variables have been developed. Artificial neural network (ANN) was employed as a software sensor for on-line estimations of state variables (biomass and substrate concentrations). A mathematical model of batch and fed batch fermentation of baker's yeast was used for training the developed ANN software sensor. IC-240 PRODUCTION OF RECOMBINANT PROTEIN BY HIGH CELL DENSITY CULTIVATION OF Pichia pastoris Anan Tongta, Kanit Nimmarairat, Yardrung Suwannarat, Penchan Mekvichitsaeng, Aluck Thipayarat, Lily Eurwilaichitr The 1 st International Conference on Fermentation Technology for Value Added Agricultural Products, March 22-25, 2005, Kosa Hotel, Khon Kaen, Thailand, p. 38 The methylotrophic yeast Pichia pastoris has been widely used for the high yield expression of various proteins by secretion into the culture supernatant or by intracellular localization. The most commonly used approach for heterologous protein expression has been to express the gene of interest under the control of the AOX 1 promoter. More than 100 heterologous proteins have been expressed using P. pastoris, at protein concentrations of up to several grams per liter. The advantages for the production of recombinant protein in P. pastoris can be summarized as high cell density fermentation, efficient secretion, a high degree of purity in the culture medium, fewer purification step and lack of endotoxins. Problems in recombinant protein production could be stated such as process consistency, reproducibility, and product optimization. To obtain high product concentration, high cell density cultivation is a chosen technique. In this International Conference

160 work, the production of recombinant human growth hormone (hGH) by high cell density cultivation of P. pastoris KM 71 was investigated. The production procedure was divided into two phases. In the first phase (high cell density cultivation), the yeast utilizes glycerol and biomass was accumulated. In the second phase (induction phase), the glycerol supply was reduced and methanol was added. Up to 200 g/1 dry weight was achieved using fed batch fermentation technique. Exponential feeding strategy was employed in fed batch experiments. Follow the fed batch period, induction phase with methanol was introduced. The amount of recombinant hGH obtained from the fermentation broth measured by SDS-PAGE and Western blot was 4.44 mg/1. IC-241 PRODUCTION OF FEED SUPPLEMENT FROM SOY BEAN PULP BY Aspergillus oryzae Anan Tongta, Montira Nopharatana, Sirirat Saartrat, Monthon Sukumprasertsri The 1 st International Conference on Fermentation Technology for Value Added Agricultural Products, March 22-25, 2005, Kosa Hotel, Khon Kaen, Thailand, p. 86 Aspergiiius oryzae is known as a microorganism that can produce digestive enzymes and fermentation stimulating factors. Whole fermentation mass (cells, enzyme, and other parts) from A. oryzae has been utilized as an active seed culture to seed the waste and animal digestive system. In this work, soy bean pulp was used as the sole substrate for the production of feed supplement using A. oryzae. Solid state fermentation (SSF) experiments had been carried out to investigate the production of digestive enzymes (amylase and protease) produced by A. oryzae. Highest amylase activity (3,6212.33 unit/g dry substrate) and highest protease activity (4,000.11 unit/g dry substrate) were found at 36 hours and 40 hours, respectively, after fermentation. The results of this study suggested that soybean pulp alone could be used as a substrate for the production of the animal feed supplement. IC-242 FRUGIVORY AND SEED DISPERSAL OF EVERGREEN FOREST VEGETATION, EASTERN THAILAND Wangworn Sankamethawee, George A. Gale KMUTT Annual Research Abstracts 2005 The 4 th International Symposium/workshop on Frugivores and Seed Dispersal “Theory and its application in a changing world”, July 9-16, 2005, Griffith University, Brisbane, Australia, p. 82 Although frugivory and seed dispersal by larger birds (hornbills) and gibbons as well as dispersal data from common fruiting trees such as Ficus spp. (Moraceae) has been documented in Thailand, there is a lack of information on general frugivory by smaller birds and other fruiting plants in this forest community. We studied frugivory by smaller birds from March 2003 to February 2005 in the 30 ha Mo Sinto Long-term Biodiversity research plot which supports over 300 species of trees and woody climbers, of which more than 50% produce fleshy fruits. Data was obtained primarily from fecal samples by mist-netting birds and from 800 hours of direct observations of 11 tree species in conjunction with phenology surveys. Additional data was collected from casual observations. A total of 46 birds species fed on 114 plant species. As expected, the defecated/regurgitated seed size was small (0.5- 16 mm). Puff-throated Bulbul (Alophoixus palidus) consumed the largest number of species (77). Frugivory activity was highest in October (14.33 visit/hr) and lowest in April (1.76 visits/hr) at the watched trees. The visitation rate varied from 0-53.2 visits/hour. Fruit removal rate was quickest in Litsea monopetala which had all their fruits removed within 10 days by 14 bird species based on diurnal observations. Fruit removal rate of the 11 observed tree species was negatively correlated with the density of conspecific trees on the plot. Our preliminary results indicate that the plant-animal interactions here are generalized, although Saurauia roxburghii seems to be dispersed almost entirely by flowerpeckers (Dicaeum ignipectus). IC-243 ASSESSING THE ROLE OF GIBBONS AS MAJOR FOOD COMPETITORS WITH HORNBILLS, AT KHAO YAI NATIONAL PARK, THAILAND Tommaso Savini, Chuti-on Kanwatanakid-savini The 4 th International Hornbill Conference, November 6-10, 2005, Limpopo, South Africa, p. 39 Hornbills and gibbons share a large role in forest regeneration as seed dispersers, due to the high frugivore component in their diet. International Conference

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    CONTENTS Page Preface International

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    National Journals

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    54 นอยกวาแบบต

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    56 (Least Square Matching Method)

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    58 อุณหภูมิ 30 แ

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    60 สเปกตรัม จา

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    62 ขอมูลดานทร

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    64 22.8 มาตรฐานเป

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    66 2545 NJ-033 EFFECTS OF ELEVATED

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    68 เปลือกที่ม

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    70 ของการดูดซ

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    72 การสกัดดวย

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    74 collection of authentic texts th

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    76 รอยละ 32 มีสา

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    210 จอมเทียนปา

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    212 การสกัดสาร

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    214 ไดแก อุณหภู

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    216 โดยกลไกของ

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    218 เคลือบเมื่

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    220 สําเริง จัก

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    222 ลดเวลาตลอด

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    224 อุณหภูมิสู

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    226 NC-043 การวิเคร

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    228 เกิดพฤติกร

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    230 เดียว เพื่อ

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    232 มากนอยเพีย

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    234 หลักที่พบใ

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    236 NC-073 การออกแบ

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    238 บทความนี้น

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    240 นําเอาเวคเ

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    242 งานวิจัยนี

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    244 การอบแหงลด

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    246 แยกเฟสต่ํา

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    248 กลบ โดยเปรี

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    250 นํามาใช คือ

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    252 วิเคราะหหา

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    254 NC-123 การวิเคร

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    256 NC-129 เครื่องผ

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    258 สามารถขยาย

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    260 ครั้งที่ 43, 1-

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    262 calculated with the use of the

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    264 Version 1.3.1 และ Softwar

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    266 ขนไกได จากก

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    268 โครงสรางขอ

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    270 การปลูกพืช

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    272 ระดับพึงพอ

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    274 ของแกสโซฮอ

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    276 1) สภาพทั่วไ

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    278 อนุปริญญา แ

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    280 อาชีวศึกษา

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    282 กระทบตอคุณ

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    284 ตั้งกลไกแข

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    286 นุชจรินทร เ

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    288 โดยรวมผูบร

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    290 แนนกระแสไฟ

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    292 NC-222 ผลงานตีพ

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    294 NC-227 เตาเผาไห

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    296 จําเพาะรวม

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    298 NC-238 จลนพลศาส

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    300 ทนงเกียรติ

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    302 ดีเซลนั้นม

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    304 NC-254 การใชเทค

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    306 อาคารศูนยก

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    308 กระบวนการร

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    310 บรรยากาศขอ

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    312 metal ions on 2-AP biosyntheis

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    314 total clones while the ethanol

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    316 การประชุมท

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    318 BioThailand (The 16 th Annual M

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    320 สูงที่สุดพ

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    322 นัยสําคัญท

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    324 ความเขมขนร

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    326 ปลูกแบบพรา

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    328 นี้ทําใหทร

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    330 ดุลชาติ มาน

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    332 ไมโครเมตร ส

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    334 In this study, we collected seq

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    336 กนกรัตน นาค

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    338 การพัฒนาสื

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    340 one of the causes of difficulty

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    342 ทวีรัตน วิจ

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    344 program, Cytoscape. This model

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    346 production demand. Nevertheless

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    348 อุลตราไวโอ

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    350 นี้อยูในชว

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    352 เพื่อนําสว

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    354 trichloroacetic acid (TCA) ม

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    356 ทรัพยากรที

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    358 ในประเทศสห

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    360 8-9 ธันวาคม 2548,

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    362 ซึ่งเกิดขอ

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    364 NC-391 การวิเคร

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    366 การประชุมว

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    368 2547) พบวามหาว

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    370 กนกพร ลีลาเ

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    Authors Index

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    376 จงจิตร หิรั

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    378 ธ ธนธร ทองส

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    380 พยุงศักดิ์

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    382 วรรณพ วิเศษ

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    384 สุดารัตน จิ

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    386 อุลาวัณย กุ

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    388 138, 141, 142 Jarunya Narangaja

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    390 Panida Kongsawadworakul 191 Pan

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    392 Sorakrich Maneewan 124, 136 Sor

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    394 KMUTT Annual Research Abstracts

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