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Preface - kmutt

Preface - kmutt

KMUTT Annual Research

KMUTT Annual Research Abstracts 2005 dology was applied to optimize the growth of P. rhodozyma. Central composite design was used to define parameters setting in this experiment. Two parameters, sucrose as carbon source and fish-soluble as nitrogen source, were studied. Results from response surface showed that optimum concentration of sucrose and fishsoluble for growth were 36.6 and 11.98 g/l. At optimum condition biomass concentration of 11.34 g/l was obtained. NC-340 FOCUSING ON GENE INVOLVING IN DEPUDECIN BIOSYNTHETIC MECHANISM FROM Xylaria SP. BCC 1067 BLOCKED MUTANTS ณมล แซเบ, จิรนันท กลอมนรา, จันทิรา ปญญา, สมชาย พงษพัฒนกิตโชติ, มรกต ตันติเจริญ, สุภาภรณ ชีวะธนรักษ BioThailand (The 16 th Annual Meeting of the Thai Society of Biotechnology: The Era of Bionanotechnology), 4-5 พฤศจิกายน 2548, ศูนยการประชุมแหงชาติสิริกิติ์, กรุงเทพฯ, หนา 260 Depudecin, a polyketide compound, has been found to be a good candidate for anticancer agent as it has a histone deacetylase (HDAC) inhibitory activity against the cancer cells, supposing it as a new strategy in human cancer therapy. However, clinical trials are ongoing with depudecin and not much information is known on the structure-activity-relationship of their derivatives and analogs. Synthesis of this compound is required but evidence on the molecular basis of the biosynthetic pathway has been limited. In order to understand biosynthetic pathway of this compound the blocked mutants that accumulate various intermediates in the biosynthetic pathways were generated by tagged DNA mediated mutagenesis. Study from the derived mutants and the retrieval of the interrupted genes by partial library and plaque screening techniques provided a few genes in the biosynthesis of polyketide. One disrupted gene fragment has 2133 base pairs coding for 711 amino acids showing the highest similarity with NADPH-cytochrome P450 oxidoreductase (CPR) of common tobacco (Nicotiana tabacum) with 84% identity. The function of this gene is electron donors for other cytochrome P450 (CYP), involving in the oxidation of functional group of polyketide compounds. All of the interrupted genes from blocked mutants will provide us a conclusion of the biosynthetic pathway of depudecin in Xylaria sp. BCC 1067. 341 Further modification of the biosynthetic pathway of this compound by gene modification will be a valuable source of new compounds with improved bioactivity pharmaceutical interest. NC-341 BLACKLEG, IMMUNOPOTENCY, MORPHOLOGY AND GROWTH ติรโภชน รัตนอมร, เพ็ญจันทร เมฆวิจิตรแสง, กนกวรรณ พุมพุทรา, แสงชัย เอกประทุมชัย BioThailand (The 16 th Annual Meeting of the Thai Society of Biotechnology: The Era of Bionanotechnology), 4-5 พฤศจิกายน 2548, ศูนยการประชุมแหงชาติสิริกิติ์, กรุงเทพฯ, หนา 184 Clostridium chauvoei is a pathogen of Blackleg. The disease affects cattle, sheep and other ruminants of ages between 6 months to 2 years associated with spore-contaminated soil. Upon infection, unless timely vaccine is provided, almost 100% mortality could be expected. Fonnalin-treated whole culture of bacteria is currently used for vaccination, therefore, high biomass concentration is desirable. Traditionally, rather low concentration of biomass, which could be similarly represented by pack cell volume (PCV), is obtained due mainly to early sporulation approximately 0.3- 0.5%. In this study, optimum growth conditions have been established to yield biomass concentration of 0.8%. Butanoi is commonly found as by-product of Clostridria spp. However, the higher the concentration of butanoi, the slower the growth and thus the faster the sporulation of C. chauvoei. It was found in this study that in batch fermentation the final concentration of butanoi could reached 3.0 mmolL -1 which is in excellent agreement with those reported in literature at which toxicity was dominant. It was also found that sporulation of C. chauvoei could be microscopically observed when the concentration of butanoi in growth exceeded 1.08 mmolL -1 . Further, immuno protective capacity of bacterial cells harvested at difference growth stages, mid-log, late log and stationary phase was also investigated. Results showed that 100% protections could be accomplished. NC-342 REMOVAL OF LIGNIN IN WASTE- WATER FROM PULP BLEACH PLANT BY USING AN IMPERFECTI FUNGUS ธเนศ องวัชรกุล, จุฑามาศ โสมสุวรรณ, อรรณพ นพรัตน, National Conference

342 ทวีรัตน วิจิตรสุนทรกุล BioThailand (The 16 th Annual Meeting of the Thai Society of Biotechnology: The Era of Bionanotechnology), 4-5 พฤศจิกายน 2548, ศูนยการประชุมแหงชาติสิริกิติ์, กรุงเทพฯ, หนา 159 An Imperfecti fungus contaminating in mushroom cultures in Biodiversity laboratory at King Mongkut's University of Technology Thon buri was found to be able to decolorize the color of effluent from a pulp bleach plant. It grew well in GYE (glucose yeast extract) medium and removed the color of the effluent (CTMP, Chemical Thermal Mechanical Process) from a pulp bleach industry up tol,380 color units (CU) in 3 days at 30°C when the initial color was 2,200 CU. This fungus can both adsorb and hydrolyze lignin in the effluent by ligninolytic enzymes. The optimal pHs for growth and decolorization were determined. The fungus grew well and decolorized the color of the effluent at pH 4.5 and 6.0, respectively. NC-343 EFFECT OF CARBON SOURCES ON LININOLYTIC ENZYME PRODUCTION AND DECOLORIZATION OF EFFLUENT FROM PULP INDUSTRIES BY Phanerochaete chrysosporium นลิน รัตนนราทร, ติยฉัตร ทองแปน, อรรณพ นพรัตน, ทวีรัตน วิจิตรสุนทรกุล BioThailand (The 16 th Annual Meeting of the Thai Society of Biotechnology: The Era of Bionanotechnology), 4-5 พฤศจิกายน 2548, ศูนยการประชุมแหงชาติสิริกิติ์, กรุงเทพฯ, หนา 158 Phanerochaete chrysosporium is widely investigated as a model white rot fungi that produces ligninolytic enzymes (Lignin Peroxi dase (Lip), Manganese Peroxidase (MnP), and Laccase). The physiological studies of enzyme production of this fungus was extensively determined in shallow cultures with synthetic medium. However, submerged cultivation is the most economic way to produce extracellular enzymes. In this study, the effect of carbon sources on Iigninolytic production of P. chrysosporium in a liquid medium, Glucose Yeast Extract, GYE [0.2 % glucose (analytical regent grade, AR) and 0.2 % yeast extract] was studied. Glucose (AR) was replaced by sucrose and glucose (commercial grade, COM) in GYE medium. All types of sugars stimulated P. KMUTT Annual Research Abstracts 2005 chrysosporium to produce Iigninolytic enzymes during its growth phase. Interestingly, glucose (COM) promoted maximum growth and Iigninolytic enzyme production. The highest activities of LiP, MnP, and Laccase in the medium with glucose (COM) were 0.65, 6.55 and 3.48 u/ml respectively. When optimal concentration of glucose (COM) was determined, the concentrations ranging between 0.5% to 1.5% were tested. It was found that 2% of glucose (COM) gave best yield of growth and enzyme production. In addition, P. chryso sporium grown in GYE could remove the color of the CTMP (Chemical Thermal Mechanical Process) effluent from a pulp industry by 1,300 color units (CU) when initial color was 2,000 CU. NC-344 γ-LINOLENIC ACID PRODUCTION BY SOLID STATE FERMENTATION OF Mucor rouxii USING SOY BEAN PULP พงศพงา จางบัว, อนันต ทองทา, มณฑิรา นพรัตน, กอบกุล เหลาเทง, สุภาภรณ ชีวะธนรักษ BioThailand (The 16 th Annual Meeting of the Thai Society of Biotechnology: The Era of Bionanotechnology), 4-5 พฤศจิกายน 2548, ศูนยการประชุมแหงชาติสิริกิติ์, กรุงเทพฯ, หนา 205 γ-Linolenic acid (GLA), a conditionally essential fatty acid, has nutritional and pharmaceutical benefits on human health. Mucor rouxii is an oleaginous filamentous fungus, which has potential in production of this economically important fatty acid. The GLA production by M. rouxii was studied using solid state fermentation (SSF). Soy bean pulp was used as a sole substrate for the fungal fermentation process. Growth and fatty acid composition were determined during cultivation of M. rouxii by indirect measurement of glucosamine and gas-chromatography, respectively. Comparing with the fatty acid profile of sole bean pulp, the novel fatty acid products appeared in the fermented mass were GLA and stearidonic acid (SDA, C18:4 ∆ 6,9,12,15 ) as a result of the fungal fermentation. This result indicated that M. rouxii could use either endogenous or exogenous linoleic acid (LA) as a substrate for GLA synthesis. In addition, the SDA accumulated in the fermented mass was the result from the transformation of exogenous α-linolenic acid (ALA) to SDA. The correlations between growth and GLA production were observed. The National Conference

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    CONTENTS Page Preface International

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    National Journals

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    54 นอยกวาแบบต

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    56 (Least Square Matching Method)

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    58 อุณหภูมิ 30 แ

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    60 สเปกตรัม จา

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    62 ขอมูลดานทร

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    64 22.8 มาตรฐานเป

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    66 2545 NJ-033 EFFECTS OF ELEVATED

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    68 เปลือกที่ม

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    70 ของการดูดซ

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    72 การสกัดดวย

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    74 collection of authentic texts th

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    76 รอยละ 32 มีสา

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    National Conferences

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    210 จอมเทียนปา

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    212 การสกัดสาร

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    214 ไดแก อุณหภู

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    216 โดยกลไกของ

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    218 เคลือบเมื่

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    220 สําเริง จัก

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    222 ลดเวลาตลอด

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    224 อุณหภูมิสู

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    226 NC-043 การวิเคร

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    228 เกิดพฤติกร

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    230 เดียว เพื่อ

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    232 มากนอยเพีย

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    234 หลักที่พบใ

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    236 NC-073 การออกแบ

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    238 บทความนี้น

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    240 นําเอาเวคเ

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    242 งานวิจัยนี

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    244 การอบแหงลด

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    246 แยกเฟสต่ํา

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    248 กลบ โดยเปรี

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    250 นํามาใช คือ

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    252 วิเคราะหหา

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    254 NC-123 การวิเคร

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    256 NC-129 เครื่องผ

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    258 สามารถขยาย

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    260 ครั้งที่ 43, 1-

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    262 calculated with the use of the

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    264 Version 1.3.1 และ Softwar

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    266 ขนไกได จากก

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    268 โครงสรางขอ

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    270 การปลูกพืช

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    272 ระดับพึงพอ

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    274 ของแกสโซฮอ

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    276 1) สภาพทั่วไ

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    278 อนุปริญญา แ

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    280 อาชีวศึกษา

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    282 กระทบตอคุณ

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    284 ตั้งกลไกแข

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    286 นุชจรินทร เ

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    288 โดยรวมผูบร

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  • Page 305 and 306: 298 NC-238 จลนพลศาส
  • Page 307 and 308: 300 ทนงเกียรติ
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  • Page 317 and 318: 310 บรรยากาศขอ
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  • Page 323 and 324: 316 การประชุมท
  • Page 325 and 326: 318 BioThailand (The 16 th Annual M
  • Page 327 and 328: 320 สูงที่สุดพ
  • Page 329 and 330: 322 นัยสําคัญท
  • Page 331 and 332: 324 ความเขมขนร
  • Page 333 and 334: 326 ปลูกแบบพรา
  • Page 335 and 336: 328 นี้ทําใหทร
  • Page 337 and 338: 330 ดุลชาติ มาน
  • Page 339 and 340: 332 ไมโครเมตร ส
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  • Page 343 and 344: 336 กนกรัตน นาค
  • Page 345 and 346: 338 การพัฒนาสื
  • Page 347: 340 one of the causes of difficulty
  • Page 351 and 352: 344 program, Cytoscape. This model
  • Page 353 and 354: 346 production demand. Nevertheless
  • Page 355 and 356: 348 อุลตราไวโอ
  • Page 357 and 358: 350 นี้อยูในชว
  • Page 359 and 360: 352 เพื่อนําสว
  • Page 361 and 362: 354 trichloroacetic acid (TCA) ม
  • Page 363 and 364: 356 ทรัพยากรที
  • Page 365 and 366: 358 ในประเทศสห
  • Page 367 and 368: 360 8-9 ธันวาคม 2548,
  • Page 369 and 370: 362 ซึ่งเกิดขอ
  • Page 371 and 372: 364 NC-391 การวิเคร
  • Page 373 and 374: 366 การประชุมว
  • Page 375 and 376: 368 2547) พบวามหาว
  • Page 377 and 378: 370 กนกพร ลีลาเ
  • Page 380: Authors Index
  • Page 383 and 384: 376 จงจิตร หิรั
  • Page 385 and 386: 378 ธ ธนธร ทองส
  • Page 387 and 388: 380 พยุงศักดิ์
  • Page 389 and 390: 382 วรรณพ วิเศษ
  • Page 391 and 392: 384 สุดารัตน จิ
  • Page 393 and 394: 386 อุลาวัณย กุ
  • Page 395 and 396: 388 138, 141, 142 Jarunya Narangaja
  • Page 397 and 398: 390 Panida Kongsawadworakul 191 Pan
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    392 Sorakrich Maneewan 124, 136 Sor

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    394 KMUTT Annual Research Abstracts

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