Preface - kmutt

344
program, Cytoscape. This model is being used to
decipher the control of GLA synthesis in M.
rouxii and to assist the experimental ationof the
important TF binding sites.
NC-347 FUNCTIONAL EXPRESSION Spirulina
∆ 12 - AND ∆ 6 -DESTURASES IN
HETEROLOGOUS YEAST, Saccharomycese
cerevisiae
พีรดา พรหมมีเนตร, อภิรดี หงสทอง, ภาวิณี เกิดฤทธิ์,
สุภาภรณ ชีวะธนรักษ
BioThailand (The 16 th Annual Meeting of the
Thai Society of Biotechnology: The Era of
Bionanotechnology), 4-5 พฤศจิกายน 2548,
ศูนยการประชุมแหงชาติสิริกิติ์, กรุงเทพฯ, หนา 246
The γ-linolenic acid (GLA) biosynthesis
in Spirulina sp. is involved with three difference
enzymes, ∆ 9 -, ∆ 12 - and ∆ 6 - desaturase which
encoded by gene desC, desA and desD,
respectively. These enzymes catalyze the fatty
acid desaturation process to yield GLA as the
final desaturation product in its pathway. In this
study, the Spirulina desA and desD desaturase
genes have been cloned and functionally
characterized by heterologous expression in
yeast, Saccharomycese cerevisiae cells. The
products of desaturation reaction were
determined by gas chromatographic and mass
spectrometric analysis of the yeast cultures. The
results indicated the growth of yeast cells with
co-expression activity of Spirulina ∆ 12 -
desaturase and ∆ 6 -desaturase and yield the final
product in this pathway, GLA. The result
revealed a possible way for Spirulina desaturases
pathway reconstitution in heterologous
yeast cells and also indicated a possibility for
using a heterologous yeast cells as a model for
functional study of Spirulina GLA pathway
reconstruction.
NC-348 FLUX ANALYSIS OF CENTRAL
METABOLIC PATHWAYS OF Saccharomyces
cerevesiae GROWN UNDER LOW
TEMPERATURE
มนตรี ฉายสวาง, กฤติญา เกรียงศักดิ์ศรี,
กอบกุล เหลาเทง, อภิรดี หงสทอง, วศิมน เรืองเล็ก,
อัศวิน มีชัย, สุภาภรณ ชีวะธนรักษ, ศักรินทร ภูมิรัตน
BioThailand (The 16 th Annual Meeting of the
Thai Society of Biotechnology: The Era of
Bionanotechnology), 4-5 พฤศจิกายน 2548,
KMUTT Annual Research Abstracts 2005
ศูนยการประชุมแหงชาติสิริกิติ์, กรุงเทพฯ, หนา 55
Cold temperature has been reported to
increase the synthesis of polyunsaturated fatty
acid (PUFA) in fungi including Saccaromyces
cerevisiae, but fairly little information regarding
cellular fluxes under such condition is available.
In this study, we investigated how low
temperature affected the intracellular fluxes
particularly on the central metabolic pathways of
S. cerevisiae, hoping to disclose the critical
enzymes controlling step that could dominate the
PUFA synthesis. The estimated cellular fluxes of
the major central pathways including the
Embden-Meyerhoff-Parnas pathway, the pentose
phosphate pathway, and the tricarboxylic acid
cycle of S. cerevisiae were compared using
metabolic flux analysis (MFA) of chemostat
culture at 15 and 30°C. The stoichiometric
model of 74 reactions and 65 metabolites
describing yeast aerobic metabolism was
constructed based on data from yeast genome
databases and literatures. Preminary results
showed that, with the equally-controlled growth
rate, the low temperature led to reduction in
rates of malate secretion but enhancing that of
ethanol and acetate. Analysis studies concerning
the calculation and comparison of the intracellular
fluxes in the central pathways of the two
temperatures aim to determine potential rate
controlling reactions. Future redirection of rate
controlling fluxes could potentially lead to an
improved precursors yield for PUFA production
in S. cerevisiae cultivation at 30 °C.
NC-349 EFFECT OF GLUCOSE
CONCENTRATION ON THE FATTY ACID
AND LIPID BIOSYNTHESIS OF Mucor rouxii
รพีพรรณ พงษเชื้อชิดไทย, กอบกุล เหลาเทง,
มรกต ตันติเจริญ, สุภาภรณ ชีวะธนรักษ
BioThailand (The 16 th Annual Meeting of the
Thai Society of Biotechnology: The Era of
Bionanotechnology), 4-5 พฤศจิกายน 2548,
ศูนยการประชุมแหงชาติสิริกิติ์, กรุงเทพฯ, หนา 252
fatty acids play the important roles as
structure and function of biological organisms.
Several factors affecting on the fatty acid
constitution have been reported. Nutrient
component mediated in fatty acid and lipid
metabolisms of many organisms. Mucor rouxii
is one of the promising sources for production of
an essential fatty acid, gamma-linolenic acid
National Conference